Name: _______________________________________ III: ______ Date: _____________ Mr.

Dan Omura

Skills Worksheet

RNA & GENE
EXPRESSION
1. Identify What are the two main steps in gene expression?
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2. Apply Concepts A DNA strand has the base sequence GCCATATTG. What is the
complementary RNA sequence?
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3. Identify Which type of RNA is produced during
transcription?____________________________________________

4. Summarize What happens during transcription?
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5. Define What is a codon?
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6. Identify Which amino acid does the codon ACU code for?
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7. Identify Give two examples of a stop codon.
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8. Describe What is the end result of
translation?________________________________________________________

9. Explain The first amino acid in most polypeptides is methionine. What is the
reason for this?
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10. Describe What causes translation to end?
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Name: _______________________________________ III: ______ Date: _____________ Mr. Dan Omura

11. Define Write a definition of gene expression in your own words.
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12. Describe What role does tRNA play in gene expression?
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13. Compare What are two differences between transcription and DNA replication?
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14. Identify What are two things that can affect the result of gene expression?
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15. Complete each statement by underlining the correct term or phrase in the brackets.
a) Transcription begins when [RNA / RNA polymerase] binds to the gene’s promoter.
b) RNA polymerase adds complementary [DNA / RNA] nucleotides as it “reads” the
gene.
c) In eukaryotes, transcription takes place in the [nucleus / cytoplasm].
16.In the space provided, write the letter of the description that best matches the term
or phrase.
____ ribonucleic acid (RNA)

____ uracil
____ ribonucleic acid
(RNA)
____ transcription
____ translation
____ gene expression

a.the entire process by which genes are used to build
proteins/traits.
b.a molecule made of linked nucleotides
c.the process of reading instructions on an RNA molecule to
put together the amino acids that make up a protein
d.the process of transferring a gene’s instructions for
making a protein to an RNA molecule
e.a nitrogenous base used in RNA instead of the base

Name: _______________________________________ III: ______ Date: _____________ Mr. Dan Omura

17. Apply Concepts Fill in the chart below to show how a DNA base sequence is
converted into a sequence of amino acids.

18.In the space provided, explain how the terms in each pair are related to each other.
a) codons, genetic code
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b) RNA, messenger RNA
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19.Determine the order in which the steps take place. Write the number of each step
in the space provided.
____ The codon following the start codon then receives the tRNA molecule with the
complementary anticodon. The
tRNA carries the amino acid specified by the codon.
____ Steps 2–5 are repeated until a stop codon is reached. The newly made protein is
released into the cell.
____The first tRNA detaches, leaves behind its amino acid, and moves away from the
ribosome.
____ Enzymes help form a peptide bond between the amino acids of adjacent tRNA
molecules.
____The tRNA (with its growing protein chain) and mRNA move one codon down, and
the next codon is ready to receive
the next tRNA and its amino acid.
____ An mRNA, the ribosome, and a tRNA carrying the amino acid methionine bind

Name: _______________________________________ III: ______ Date: _____________ Mr. Dan Omura

together. The tRNA bonds to the
“start” codon AUG.