Objectives on the Nikon - Properties Magnification 4 10 20 40 60 100

Resolution: Z depth: Brightness:

N.A. 0.2 0.45 0.75 0.95 1.4 1.4

Resolution (mm) 1.5 0.7 0.4 0.3 0.2 0.2

Z depth (mm) 25 4.9 1.8 1.1 0.8 0.8

Brightness 1 4.1 7.9 5.1 10.7 3.8

Closest distance between two objects that can be separated How thick each z-slice is (changes slightly for different wavelengths) (calculated for 500nm light) For fluorescence, 60x is best, followed by 20x

0 11.2 depth of field (mm) 100.9 Brightness 0.4 0.6 0.Properties Magnification N.3 Resolution: Depth of field: Brightness: Resolution (mm) 3.3 2.1 4.4 0.8 1.0 0. 4x 0. 40x is best.A.1 1.9 Closest distance between two objects that can be separated What thickness of the specimen is in focus at a given point For fluorescence.Zeiss Objectives .5 40 x 0.3 20 x 0.1 10 x 0.85 100 x 1.1 0. followed by 100x .0 1.6 3.