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WHO_LEPTO

WHO_LEPTO

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Published by: egalivan on Jun 19, 2009
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05/11/2014

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The methods commonly used to differentiate between saprophytic and pathogenic leptospires are:
growth in the presence of 8-azaguanine (225 mg / l) (Johnson & Rogers, 1964);
growth at 13 °C (Johnson & Harris, 1967);
conversion to spherical forms in 1M NaCl (Johnson & Faine, 1984).

Growth at 13 °C in the presence of 8-azaguanine and conversion to spherical forms in 1M NaCl suggests that
leptospires are saprophytes. The ELISA test, in which only antigens of pathogenic leptospires react to monoclonal
antibody F9-4 (Cinco, 1990), can also be used.

Some PCR-based techniques have been developed which will distinguish between pathogenic and saprophytic
Leptospira occurring in water (Murgia et al., 1997). The ability to distinguish between pathogenic and saprophytic
leptospires in the environment can be of value for epidemiological and public health purposes.

REFERENCES

M. Cinco (1990). Evaluation of monoclonal antibody F9-4 as immunological probe for Leptospira interrogans.
Journal of Clinical Microbiology, 28:2154-2155.

R.C. Johnson, S. Faine (1984). Order I. Spirochaetalis: Family II. "Leptospiraceae" Hovind-Hougen 1979.
In: Krieg NR, Holt JG, eds. Bergey's manual of systematic bacteriology, 1st ed. Baltimore, MD, Williams & Wilkins,
Vol.1, 245: 62.

R.C. Johnson, V.G. Harris (1967). Antileptospiral activity of serum. II. Leptospiral virulence factor. Journal of
Bacteriology, 93:513-519.

R.C. Johnson, P. Rogers (1964). Differentiation of pathogenic and saprophytic leptospires with 8-azaguanine.
Journal of Bacteriology, 88:1618-1623.

R. Murgia, N. Riquelme, G. Baranton, M. Cinco (1997). Oligonucleotides specific for pathogenic and saprophytic
leptospira occurring in water. FEMS Microbiology Letters, 148:27-34.

ANNEX 8
SEROTYPING AND PREPARATION OF RABBIT ANTISERUM

55

ANNEX 8

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