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LAPORAN PRAKTIKUM BIOKIMIA SEMESTER GENAP 2012/2013

Kelompok Nama

: 4, 14, dan 15 : 1). Asyari Fauzan 2). Farha Kamila 3). Guruh Mehra 4). Sesilia Genesy 5). Sri Ayu Lestari 6). Windy Rizkaprilisa (110 )

(1106 ) (1106 ) (110 )

(1106020705) (1106008896)

LABORATORIUM DASAR PROSES KIMIA DEPARTEMEN TEKNIK KIMIA FAKULTAS TEKNIK UNIVERSITAS INDONESIA DEPOK, 2013

I. Concept This attempt gets quantitative character, where praktikan must measure protein absorbance of asample to account its concentration. This absorbance measurement did by spektrofotometris method. II. Technique Technic that is utilized to measure protein absorbance is spektrofotometri. Spektrofotometri is one of method in chemical analisis who is utilized to determine composition a good sample according to quantitative and kualitatif which is gone upon on interaction among material with light. Spectrophotometer consisting of : lominous source monokromator sample cell detector read out (reader). Process Light Absorbtion on Spektrofotometri While light with length various wavelength (polikromatis's light) about a substance, therefore light with wavelength one particular that will be absorbed. In a molecule that holds to play a part essential is valence electron of each aught atom until be formed a material. Proprietary electron by a molecule get to devolve (excitation), pivot (rotasi) and chatters (vibrasi) if is hit an energy. If substance absorbs observable light and UV therefore will happen electron transfer of ground state makes towards situation most excitation. This electron transfer

disebut transisi elektronik. If permeated light is infrared light therefore aught electron in atom or cords electron at one particular molecule can will only chatter (vibrasi). Meanwhile electron pirouetting happens on energy yet lower level e.g. on radio wave. On a basic this is spektrofotometri is designed to measure concentration a deep aught a sample. Where is substance whatever available in sample cell is irradiated with light that have particular wavelength. While are light hit sample plays favorites will be absorbed, partly will be disseminated and partly again will be kept on.