PEMAKAIAN KLINIS AMNION

Menkher Manjas MD, FICS
DR. M DJAMIL HOSPITAL TISSUE BANK Perintis Kemerdekaan Street PADANG WEST SUMATERA INDONESIA Ph/Fax + 62 751 30706

SELAPUT AMNION
  Selaput luar ketuban diatas chorion Tipis & transparan


Fungsi  Proteksi, nutrisi janin
Mengandung bahan- bahan nutrisi dan antiseptik untuk bayi

Dimanfaatkan  Diambil lapisan amnionnya  Biomaterial .KETUBAN 1. Tidak dimanfaatkan  Dibuang  Dikubur  Dihanyutkan 2.

Bentuk : tipis . Mengandung zat kimia 1. elastis . 3. Transferin   Mencegah rx imun Punya faktor angineogenik 2. transparan 2.AMNION 1. Allantoin & Lisozome  bersifat bakteriostatik Progesteron . Sifat : Permeabilitas terhadap O2 & CO2 3.

SEDIAAN AMNION 1. Amnion radiasi (Radiation sterilized lyophilized amniotic membrane) . Amnion Segar 2.

Amnion Segar  Amnion yang langsung diambil dari placenta diolah dan dipergunakan untuk klinis  Bertahan hanya untuk lebih kurang 1 minggu  Tidak steril .

Amnion Radiasi   Amnion yang sudah diproses pada bank jaringan Sterilisasinya secara radiasi (Radiation sterilized lyophilized amniotic membrane)   Dapat disimpan dalam waktu lama Dapat dipergunakan kapan saja .

Lyopilisasi  pengeringan pada kondisi beku tanpa melewati fase cair shg tak merusak fisik dan biokimia jaringan 5. Packing KGy 6. Pengambilan ketuban 2. Radiasi  sterilisasi dengan sinar gamma 25 . Pelepasan amnion 3.Tahap Proses Amnion Radiasi 1. Pencucian 4.

Kegunaan Klinis Amnion 1.Penutup Luka (Redresing Luka) (Biological Wound redressing) 2.Allograft .

Ulkus Diabetikum 3. Decubitus 4. Luka terbuka 5.Amnion Sebagai Redresing 1. Penutup luka paska operasi / Sirkumsisi . Luka bakar 2.

Urethroplasty . Penutup membrana tympani 4. Pasca operasi mata 3.Amnion Sebagai Allograft 1. Vaginoplasty 5. Penutup luka skin graft 2.

Mengurangi rasa nyeri 5. Tidak ada Resistensi dari tubuh 2. Kemampuan angiogenik  p darah baru 4. Menekan pertumbuhan kuman (Invivo /invitro) .Amnion Sebagai redresing Luka 1. Mempercepat penyembuhan luka 3. Pemakaian lama  side efek (-) 6.

Keunggulan Amnion sebagai redresing luka  Lengket dengan luka  mengurangi evaporasi cairan . protein dan panas  Elastis & lunak  Tanpa perlu redresing skunder  Dapat dihancurkan oleh kuman  Tersedia dan tersimpan lama  Amnion radiasi .

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. mengolah jaringan donor dan selanjutnya memproses sehingga dapat dipergunakan untuk kepentingan pengobatan.TISSUE BANK RSUP DR M JAMIL PADANG Satu instalasi di RS yang bertanggung jawab dalam mencari.

 1993  Penelitian amnion radiasi (BATAN)  1996-1997  Bantuan sarana dari IAEA & BRTB  1994  Penataran tenaga (Dalan & Luar negeri).  1 Juli 1997  Produksi pertama  28 Oktober 1998  diresmikan oleh Menkes .SEJARAH BERDIRINYA TB M DJAMIL  1972  Amnion segar  LB derajat II.

2. Processing . Sterilisasi alat-alat kesehatan 3. Research   Medical  Non medical . storage and distribution of human tissues for medical uses.TISSUE BANK ACTIVITIES 1.

TISSUE BANKING SYSTEM. 2000 NATIONAL NETWORK IN INDONESIA CIPTO HOSPITAL JAKARTA SOETOMO HOSPITAL TISSUE BANK SURABAYA  50 HOSPITAL FATMAWATI HOSPITAL JAKARTA BATAN RESEARCH TISSUE BANK (BRTB) SUBROTO ARMY HOSPITAL JAKARTA SIAGA RAYA ORTHOPAEDI HOSPITAL JAKARTA M DJAMIL HOSPITAL TISSUE BANK PADANG 6 HOSPITAL National Coordinator EYE HOSPITAL IN JAKARTA AND AROUND INDONESIA Other : PALEMBANG. MAKASAR. information. MALANG JOGJA LEPROSY HOSPITAL TANGERANG Consultations and raw material Finished products.s and training Consultation. irradiation and information .

Nursal Hasbi Pemakaian klinis Amnion radiasai pada luka bakar 1995 Jum kasis : 30 pasien luka bakar Kesimpulan Amnion merangsang pertumbuhan jaringan epitel kulit. Presented at Asian Surgical Association 10th Bienial Congress 12-16 March Bali Indonesia .Pemakaian Amnion pada Luka bakar Kamardi Thalut. jaringan granulasi dan pertumbuhan kapiler baru.

o.  No allergic reaction Presented at 8th International Conference On Tissue banking in cooperation with International Atomic energy October 22-24. Menkher Using amniotic membrane as wound covering after caesarean section operation 2000 No of case : 196 pregnant women Age : 26 to 38 y. 2000 Bali Indonesia .Pemakaian Amnion Pada Penutupan Luka pasca Sectio Caesaria Helfial Helmi. Conclusion Amnion (as wound covering post cesarean) Overall results are good  No severe complication  No wound dehiscence.

Conclusion Amnion (as wound covering post circumcision):  No complication (Local   Simple method Available infection & Allergic Reaction) Presented at 8th International Conference On Tissue banking in cooperation with International Atomic energy October 22-24. Doddy Efmansyah Experience of using amniotic after circumcision No of case : 165 boys Age : 6-10 yo. 2000 Bali Indonesia . Ismal.Pemakaian Amnion Sesudah Sirkumsisi Menkher.

Pemakaian Amnion Sesudah Sirkumsisi Yulfirsta . Erkadius Pemakaian amnion sebagai penutup luka pasca sirkumsisi (2001) Jum kasus Umur : : 16 boys 6-10 yo. Doddy Efmansyah. Kesimpulan Amnion sebagai penutup luka pasca sirkumsisi  Mengurangi nyeri  Tidak perlu redresing ulang  Langsung mobilisasi Final paper as surgery training Medical Faculty Andalas university 2001 .

(1999) Jumlah kasus 52 pasien Kesimpulan Amnion mempercepat penyembuhan terbentuknya jaringan keloid . luka dan mengurangi .Pemakaian Amnion Pada Skin Graft Arsil Hamzah . Menkher Manjas Studi perbandingan pemakaian amnion dengan sofratulepada luka donor skin graft.

Amnion For Skin Graft Menkher Manjas . Arsil Hamzah Comparison studies of using radiation amnion and sofratule in host of skin graft. . (1999) No of cases 52 patients Conclusion Amnion promoted wound healing and scar formation.

Ibrahim S Department of Ophthalmology Faculty of Medicine Andalas University/M Djamil Hospital .Multilayered Amniotic Membrane Transplantation for the Treatment of Corneal Epithelial Defects and Ulcers Getry Sukmawati.

.H. FICS. Sp. MENKHER MANJAS. Sp.OT.B.PEMAKAIAN AMNION LYOPHILISASI STERIL RADIASI SEBAGAI PERBAN BIOLOGIS PADA TUKAK DIABETES JEFRI HENKY Dr.

2000 Seoul Korea . Nazly Hilmy Rejection study of cancelous allograft in emergency orthopedic operation (2000) No of cases : 40 patients Conclussion Allograft significantly increased callus formation. Presented at 8th International Conference On Tissue banking in cooperation with International Atomic energy October 22-24 . Nazly Hilmy Comparison studies on rejection and bone healing of radiation sterilized cancelous allograft and xenograft in orthopedic operation (2002) No of cases 64 patients Conclusion  Xenograft and allograft. Presented at 8th International Conference On Tissue banking in cooperation with International Atomic energy October 22-24 .2000 Bali Indonesia Menkher Manjas. have same effect of increased callus formation.Clinical Application Of Bone graft Menkher Manjas.

Of Pathologic Anatomy Faculty University Padang INDONESIA’’ Of Medicine Andalas 11th International Conference Asia Pacific Association Of Surgical Tissue bank 24th-26 th Nov 2006 Mumbai India . Dept.IS AMNION CREAM ALSO EFFECTIVE IN COLLAGEN FORMATION FOR WOUND HEALING (WISTAR RATS WOUND EXPERIMENTAL STUDIES) ‘Menkher Manjas Fathar Usman’ ‘’Salmiah Agus’’ ‘ Dr. Of Surgery Faculty Of Medicine Andalas University Padang INDONESIA’ ‘’ Dept. M Djamil Hospital Tissue Bank.

Jumniah (Post graduate Agriculture Faculty Sriwijaya University1999) Radiation effect of soybean in increasing production 3. Henni Agusmira (Agriculture Faculty Andalas University1999) Effect of Cobalt 60 type radiation for Aspergilus mutan to increase citric acyd production from residual tapioca 2. Yopi Sartika (Faculty Of Chemistry Andalas University 2000) Over production of citric acyd after radiation glukosamine mutan aspergilus niger 4. Susi Djamhari ( Internal Medicine Department Medical Faculty Andalas University 2000) Comparison studies of freeze chondroitin sulfat and piroksikam in osteoarthritis of the knee .Non Medical Research 1.

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