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Published by Ahmer Israr
Blast Lab
Blast Lab

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Published by: Ahmer Israr on Oct 07, 2013
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We were instructed to use the NCBI BLAST website to select and compare a gene in different randomly selected taxa and study the gene’s functions pertaining to each taxa and whether this function varies. The importance of studying this particular gene is to determine it’s functional variation and diversity of use. This study investigates the evolutionary relevance of the gene “adenosine aptamer gene”, in relation between randomly selected taxa. The results should allow us, in researching the gene, to make a comparison of the gene’s functions between the taxa. A comparison of the gene’s DNA structures between the taxa on the NCBI Blast website indicates that the adenosine aptamer gene will most likely function relatively similarly between the taxa, which would suggest that the gene was beneficial to many organisms throughout the course of evolution, as the gene has not undergone more than a few base pair changes. Introduction

These genes are particularly useful to manipulate and control protein function in the context of living cells or organisms. They function as checks on protein expression and manifest themselves in slightly different ways in different organism, accounting for the general usability of the genetic coding for specific functions with little modification. For this particular study, we were given the objective to study the aptamer gene and its varying functions between humans and 7 other randomly selected taxa. The main objective is to understand this active gene’s relevance to evolution within the selected taxa.To further understand the evolutionary relevance of the aptamer gene, we compared the DNA structure of the gene in humans in relation to the randomly selected taxa.The research questions driving this study are as follows: 1 ● How does this gene differ in function (if it differs at all) between the taxa? 2 ● What is the evolutionary relevance of this gene in each different taxa? To investigate these questions, we utilized the NCBI BLAST website to compare and contrast the DNA structures of the gene in one taxa to another. The rest of the article is structured as follows: First, a description of the research methods and procedures used in the study will be listed. Results of the study follow. Finally, implications, limitations, and directions for future research are offered. Methodology We used the NCBI website (http://www.ncbi.nlm.nih.gov/) to select a gene. We chose the adenosine aptamer gene. We then entered the gene into the NCBI database to find the nucleotide sequence to enter into the BLAST website (http://blast.ncbi.nlm.nih.gov/Blast.cgi). The NCBI and BLAST websites were our main sources of information for this lab, as no other direct research methods were available.


humans.BLAST LAB 3 General cladogram Discussion After extensively studying this gene. including frogs. In the future. They may not necessarily be because of close relation though. and bacterium. we found that this gene is located across many taxa. it may be interesting to examine how exactly these genes stay intact and remain similar without having relation. One possible explanation may be convergent evolution. which every organism does. this gene produces aptamers that bind to molecules. .

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