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Examples of Bacterial Identification

Examples of Bacterial Identification

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Published by Chai Yawat

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Published by: Chai Yawat on Oct 16, 2013
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Identification of bacteria

เอนก ภู่ทอง ภาควิชาเทคนิคการแพทย์ คณะสหเวชศาสตร์ มหาวิทยาลัยธรรมศาสตร์

Biochemical test for bacteria

เอกสารนี้  แสดงการอ่านและแปรผลการทดสอบเชื้อแบคทีเรีย  จัดทาขึ้นเพื่อเป็นบันทึกช่วยในการเรียนรู้ของนักศึกษาเทคนิค การแพทย์

Staining .

Gram’s stain Gram negative Gram positive appearance Purple Pink to red interpret Gram positive Gram negative .

Appearance on bacteriological agar .

Sheep blood plate Alpha-hemolysis Beta-hemolysis Gamma-hemolysis .

MacConkey agar colony 1 2 3 4 appearance pink colony pink colony no growth colorless colony interpret Lactose fermenter Lactose fermenter Non lactose fermenter .

Shigella-Salmonella Agar (SS agar) Pink colony Colorless colony with black center colony 1 2 appearance pink colony Colorless colony Colorless colony interpret Lactose fermenter Non Lactose fermenter 3 Colorless colony with black center Non Lactose fermenter H2S producing colony .

cholera on TCBS Green colony colony 1 2 appearance Green colony Yellow colony interpret Non sucrose fermenter Sucrose fermenter .TCBS Yellow colony V.parahaemolyticus and V.

biochemical test .

4 O2 K/N Non lactose fermenter Dextrose Mixed acid O2 A/A Initial reaction Dextrose Mixed acid O2 K/A Delayed reaction lactose fermenter Dextrose + Lactose Mixed acid O2 A/A .Triple Sugar Iron Agar (TSI) Non fermenter No fermentation pH= 7.

H2S 1 Yellow Yellow + A/AG 2 red yellow + K/AG 3 red yellow + K/A+ 4 red neutral K/N .Triple Sugar Iron Agar (TSI) 1 2 3 4 -Fermentation Yellow ~Acid (A) Red~Alkaline (K) No change ~Neutral (N) -Gas positive  G -H2S positive  + tube appearance Slant Butt Gas H2S interpret Slant/butt. gas.

Lysine Iron Agar (LIA) Slant  Lysine Deaminase (LDA) purple = negativenon LDA producer red=positive LDA producer Butt Lysine Decorboxylase (LDC) yellow =negative non LDC producer purple = positive LDA producer tube appearance Slant Butt H2S interpret Slant/butt control purple purple 1 purple yellow -/- 2 red yellow +/- 3 purple purple -/+ 4 purple purple + -/+ .

motile 1 -/-/- 2 + + -/+/+ 3 + + + +/+/+ .SIM 1 2 3 tube sulfur indole motile Sulfur. indole.

To tests the ability of organisms to utilize citrate as a carbon source .Sodium citrate is the sole source of carbon 1 2 tube appearance bacteria 1 No growth 2 growth agar interpret Not change - Blue + .Simmon’s Citrate agar .

Tolerance testing .

Bacitracin susceptibility test Clear zone  susceptibility positive No clear zone  resistance negative .

5% NaCl control appearance interpret clear - 1 clear Negative (-) 2 turbid Positive (+) .1 2 6.

Immunological testing .

Anti-serum agglutination test 1 2 1 appearance interpret Non agglutinate Negative (-) 2 agglutinated Positive (+) .

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