Fig 2 HPLC/RI chromatography of Ginkgo terpene trilactones extracted from a Ginkgo leaf sample. Ginkgolides ! "! C! #!

and $ilo$alide are a$$re%iated as G ! G"! GC! G#! and ""! respecti%ely. I&'( represents the internal standard! $en)yl alcohol

&eparation of the fla%onols -as achie%ed at 203C on a mini$ore Phenomenex L*na 0mm C45 627 col*mn -ith dimensions 201 x 2. /01nm. 8o$ile phase 6acetonitrile7 and " 61./9 formic acid7 ratios -here changed after 40 min*tes from 40+50 to 20+:0 and total r*n time -as // min*tes.rate of 211ml/min. one at the start of the analysis. a y = peak area.11mm *sing a one step linear gradient and flo. each concentration injected 3 times. Table 1: Linear ranges and correlation coefficients of calibration curves (Day 1). x = concentration n = number of points in the calibration curve.Fig*re 2+ HPLC chromatogram of reference standards! r*tin! .*ercitrin! .*ercetin! kaempferol and isorhamnetin -ith corresponding retention times at l . b . one in the middle and one injection after the tablet assay was completed.

Figure 3: Overlaid HPL c!ro"atogra"s of t!e reference standards including t!e #rofiles of t!e analysed Ginkgo biloba dosage for"s ($ % 3&'n").IR spectra of c*ticles.HPLC analysis of 0 Ginkgo biloba preparations ga%e remarka$ly similar fingerprint profiles 6Fig*re /7 indicating higher amo*nts of r*tin compared to the other fla%onols. 6a7 Ginkgo $ilo$a 6Recent7< 6$7 Ginkgo adiantoides 68aastrichtian! late Cretaceo*s7< 6c7 Ginkgo co%iacea 6Cretaceo*s7< 6d7 $ietites linkii 6=ealden! early Cretaceo*s7< 6e7 Frenelopsis 6"arremian! early Cretaceo*s7. 'ransmission F'. .

>spectro del "ilo$alido ?@A@I& .

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