Master project at the Department of Forest Mycology and Plant Pathology, as soon as possible.

The Paenibacillus polymyxa and Bacillus thuringiensis.genomes have been recently sequenced at Uppsala Genome Centre using Ion Torrent PGMTM systems. The P. polymyxa sequence reveals that it harbours nine giant gene clusters directing synthesis of bioactive peptides (antibiotics) and polyketides by modularly organized megaenzymes with the names non-ribosomal peptide synthetases, (NRPS), and polyketide synthetases, (PKS). Comparative genomics with the significantly inferior biocontrol agent reveals 94,6 % homology, the main difference being a unique 58KB region including novel potential NRPS/PKS combinations. Your task would be working on the region and identifying the potentially novel compounds.

The research is performed in parallel with bioassays and antagonism assays. Compounds will be separated by HPLC and TLC bioautography. The active fractions will be sequentially used for HPLC-ESI-MS and MALDI-TOF_MS identification and characterization. We are looking for a motivated student. If you are interested do not hesitate to contact:
Salme Timmusk Associate professor/Docent Salme.timmusk@slu.se Postal address: Uppsala BioCenter Dept. of Forest Mycology and Plant Pathology: P.O Box 7026 SE-75007 Uppsala Sweden Visiting address: Uppsala BioCenter Dept. of Forest Mycology and Plant Pathology Almas allé 5 Phone: +46(0)737015402 SLU, Uppsala Sweden

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