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Lampiran 1.

Bagan Kerja Penelitian

Buah sawo muda (1,3 kg) Dibersihkan dan dicuci Ditiriskan dan ditimbang (1,1 kg) Dipotong kecil-kecil Dikeringkan dalam lemari pengering Simplisia Dihaluskan Ditimbang Ditimbang Serbuk simplisia 280 g

karakterisasi simplisia

Skrining fitokimia

Pembuatan ekstrak buah sawo Diperkolasi dengan etanol 70%

 Makroskopik  Mikroskopik  PK air  PK sari larut air  PK sari larut etanol  PK abu total  PK abu tidak larut asam

 Alkaloid  Flavonoid  Glikosida  Tanin  Saponin  Steroid/triterp enoid

Perkolat Dipekatkan dengan alat rotary evaporator Dikeringkan dengan freeze dryer Ekstrak kental (151,132 g)

Uji efek

Universitas Sumatera Utara

Lampiran 2. Tumbuhan Sawo a. Tanaman Sawo (Achras zapota L.)

b. buah sawo

1 cm

Universitas Sumatera Utara

Lanjutan Lampiran 2

c. simplisia buah sawo

1 cm

Universitas Sumatera Utara

Hasil Identifikasi Tumbuhan Universitas Sumatera Utara .Lampiran 3.

Mikroskopik Serbuk Simplisia Buah Sawo 1 2 3 4 Keterangan : 1.Lampiran 4. sel-sel batu 4. pigmen berwarna coklat Universitas Sumatera Utara . serabut sklerenkim 2. endosperm dengan butir pati 3.

8 x 100 % = 16.7 ml = 0. Berat sampel I Berat sari air Kadar sari yang larut dalam air = = 4.33% Perhitungan Penetapan Kadar Sari yang Larut Dalam Air Persen kadar sari yang larut dalam air = Berat g sari air 100 x x 100 % Berat simplisia 20 a.00 % 3 = 15.000 g = 0.003 g = 0.7 x 100 % = 14. Berat sampel I Volume air Kadar air b.99 % + 14.51 % 4. Perhitungan Hasil Pemeriksaan Karakterisasi Simplisia Perhitungan Penetapan Kadar Air Kadar air simplisia = a.999 20 b.999 g = 0.000 = 5.371 g Universitas Sumatera Utara .00 % 5.000 Kadar air rata-rata = 15.00 % + 16. Berat sampel II Volume air Kadar air c.000 g = 0. Berat sampel III Volume air Kadar air Volume air x 100 % Berat sampel = 5.99 % 5.385 g 0.8 ml = 0. Berat sampel II Berat sari air = 5.385 100 x x 100 % = 38.001 = 5.Lampiran 5.000 g = 0.8 x 100 % = 15.8 ml = 0.00% 5.

366 g 0.Lanjutan Lampiran 5 Kadar sari yang larut dalam air = 0. Berat sampel II Berat sari etanol = 5.58 % + 36.00 % = 38.003 20 Kadar sari yang larut dalam etanol = c.1 0% + 38.002 g = 0.38 x x 100 % = 38.366 100 x x 100 % = 36.88 % + 36.000 20 c.01 % 3 Perhitungan Penetapan Kadar Sari yang Larut Dalam Etanol Persen kadar sari yang larut dalam etanol = Berat g sari etanol 100 x x 100 % Berat simplisia 20 a.003 20 Kadar sari yang larut dalam etanol = Kadar sari rata-rata = 38.88 % 5.369 g 0.369 100 x x 100 % = 36.58 % 5.002 20 Kadar sari yang larut dalam etanol = b.10 % 5.000 20 Kadar sari yang larut dalam air = Kadar sari rata-rata = 38. Berat sampel III Berat sari air = 5.88 % 3 = 37.51 % + 37. Berat sampel I Berat sari etanol = 5.38 g 100 0.371 100 x x 100 % = 37.88 % 5.389 100 x x 100 % = 38. Berat sampel III Berat sari etanol = 5.003 g = 0.389 g 0.00 % 5.45 % Universitas Sumatera Utara .000 g = 0.003 g = 0.

0002 = 2.0005 g = 0.0196 g Persen kadar abu tidak larut asam = 0. Berat sampel I Berat abu Persen kadar abu total b.0005 g = 0.96% + 1.0004 Persen kadar abu total Kadar abu total rata-rata = 1.0392 g = 0.0388 g = 0.96% 2.0388 x 100 % = 1.0002 g = 0. Berat sampel I Berat abu = 2.94% + 1.77 % 2.0392 x 100 % = 1.0196 x 100 % = 0.77% = 1.0174 g Universitas Sumatera Utara .98 % 2. Berat sampel II Berat abu = 2.0354 x 100 % = 1.0004 g = 0.0002 g = 0.89 % 3 Perhitungan Penetapan Kadar Abu yang Tidak Larut Dalam Asam Persen kadar abu tidak larut asam = Berat g sisa hasil x 100 % Berat sampel a.0005 b. Berat sampel III Berat abu Berat abu x 100 % berat sampel = 2.94 % 2.Lanjutan Lampiran 5 Perhitungan Penetapan Kadar Abu Persen kadar abu total = a.0354 g = 0. Berat sampel II Berat abu Persen kadar abu total c.0005 = 2.

0004 g = 0.Lanjutan Lampiran 5 Persen kadar abu tidak larut asam = 0.88 % 2.88% =0.0004 Kadar abu tidak larut asam rata-rata = 0.0002 c.0176 x 100 % = 0.87% + 0.87 % 2.0174 x 100 % = 0.91% 3 Universitas Sumatera Utara .0176 g Persen kadar abu tidak larut asam = 0.98% + 0. Berat sampel III Berat abu = 2.

Hewan Percobaan a.Lampiran 6. Mencit yang Mengalami Diare f Keterangan : f = feses mencit dengan konsistensi berlendir/berair tidak membentuk massa feses Universitas Sumatera Utara . Mencit dalam Keadaan Sehat b.

Wadah Pengamatan b.Lampiran 7. Mencit dalam Wadah Pengamatan dan Kandang Mencit a. Mencit dalam Kandang Universitas Sumatera Utara .

Feses Mencit dengan Berbagai Konsistensi a b c d Keterangan: a = konsistensi feses normal b = konsistensi feses lembek c = konsistensi feses berair/berlendir membentuk massa feses d = konsistensi feses berair/berlendir tidak membentuk massa feses Universitas Sumatera Utara .Lampiran 8.

5 4 4 4 0 0 4 4 0 0 3 4 0 0 0 0 0 Ulangan III IV 0 0 1 0 0 0 0 1 1 0 0 3 0 3.Lampiran 9.67 2 0 3.67 4 4 4 4 4 0 4 4 0 0 0 0 4 4 0 3 I 0 0 0 0 1 0 0 1 0 0 1 0 I 0 0 0 2.5 4 4 4 0 0 0 0 0 II 1 0 0 0 0 0 1 0 0 0 0 1 II 1 0 0 0 3 3.5 4 4 0 0 0 0 0 0 0 I II 0 0 1 1 0 0 3.5 4 0 4 4 0 4 4 0 VI 1 0 0 0 0 1 0 0 0 0 1 0 VI 0 1 0 0 3 4 0 0 0 4 4 0 Kelompok II Loperamid HCl Kelompok III EEBS 1 g/kg BB Universitas Sumatera Utara . Hasil Pengujian Efek Antidiare a.67 0 0 0 4 1 0 0 0 0 0 Ulangan III IV 0 1 0 1 0 3 1 4 1 4 2 4 3 0 3.5 4 4 4 4 4 0 0 0 0 VI 0 0 0 3.5 4 4 0 4 0 0 V 0 1 0 4 4 0 0 4 4 0 4 0 V 0 0 0 0 1 1 0 0 0 0 0 0 V 0 0 3. Konsistensi Feses Mencit setiap 30 Menit Kelompok I Kontrol negatif Waktu (menit ke) 30 60 90 120 150 180 210 240 270 300 330 360 Waktu (menit ke) 30 60 90 120 150 180 210 240 270 300 330 360 Waktu (menit ke) 30 60 90 120 150 180 210 240 270 300 330 360 Ulangan III IV 0 0 0 0 3.5 3.

5 4 4 0 0 0 0 0 II 0 1 0 1 0 0 0 0 0 0 0 0 Ulangan III IV 0 0 0 0 3 0 4 0 4 1 0 4 4 4 0 0 4 0 0 0 0 0 0 0 Ulangan III IV 0 1 0 0 1 0 0 0 0 1 0 0 3.5 g/kg BB Universitas Sumatera Utara .5 4 4 0 0 3 0 0 I 1 1 0 1 0 0 1 0 0 0 0 0 II 0 0 0 0 3.5 0 4 1 0 0 0 0 4 0 0 0 V 0 0 0 0 0 0 1 0 1 0 0 0 V 0 0 0 1 0 0 0 0 0 0 0 1 VI 0 1 0 3.5 4 0 4 0 4 0 0 0 VI 0 1 0 0 0 0 0 0 0 1 0 0 Kelompok V EEBS 2.Lanjutan Lampiran Kelompok V EEBS 2 g/kg BB Waktu (menit ke) 30 60 90 120 150 180 210 240 270 300 330 360 Waktu (menit ke) 30 60 90 120 150 180 210 240 270 300 330 360 I 1 0 0 0 3.

17 0.33 2.17 0.83 0.50 0.17 0.16 1.67 0.Lanjutan Lampiran 9 b.50 2.67 0.00 0.67 0.00 I 0 1 0 2 3 3 2 2 0 0 2 0 I 0 0 0 0 2 0 0 1 0 0 1 0 I 0 0 0 2 3 4 3 0 0 0 0 0 II 0 1 0 3 3 4 0 4 0 0 2 1 II 1 0 0 0 0 0 1 0 0 0 0 1 II 1 0 0 0 3 2 4 4 0 3 0 0 Kelompok II Loperamid Kelompok III EEBS 1 g/kg BB Universitas Sumatera Utara . Frekuensi Defekasi Mencit Kelompok I Kontrol negatif Waktu (menit ke) 30 60 90 120 150 180 210 240 270 300 330 360 Waktu (menit ke) 30 60 90 120 150 180 210 240 270 300 330 360 Waktu (menit ke) 30 60 90 120 150 180 210 240 270 300 330 360 Ulangan III IV 0 0 0 0 3 2 0 3 2 3 4 0 0 4 3 0 0 3 1 0 0 0 0 0 Ulangan III IV 0 0 1 0 0 0 0 1 1 0 0 3 0 3 0 0 0 4 1 0 0 0 0 0 Ulangan III IV 0 1 0 1 0 2 1 3 1 3 3 2 3 0 3 1 2 0 0 0 0 0 0 0 Rata-rata V 0 2 0 4 4 0 0 2 2 0 1 0 V 0 0 0 0 1 1 0 0 0 0 0 0 V 0 0 2 3 2 2 3 1 0 0 0 0 VI 0 0 0 2 4 0 3 3 0 2 2 0 VI 1 0 0 0 0 1 0 0 0 0 1 0 VI 0 1 0 0 2 4 0 0 0 4 3 0 0.33 0.33 0.17 0.00 0.83 2.67 0.17 1.50 1.17 0.50 0.17 Rata-rata 0.17 0.33 0.33 0.67 1.17 Rata-rata 0.50 2.83 1.83 2.33 0.83 0.33 1.33 3.

17 2.343 0.33 1.67 0.640 Ulangan II III IV 0.5 g/kg BB (menit ke) 30 60 90 120 150 180 210 240 270 300 330 360 I 1 0 0 0 2 3 3 0 0 3 0 0 I 1 1 0 1 0 0 1 0 0 0 0 0 II 0 0 0 0 3 4 4 0 0 0 0 0 II 0 1 0 1 0 0 0 0 0 0 0 0 Ulangan III IV 0 0 0 0 2 0 4 0 4 1 0 4 2 3 0 0 1 0 0 0 0 0 0 0 Ulangan III IV 0 1 0 0 1 0 0 0 0 1 0 0 2 0 4 1 0 0 0 0 3 0 0 0 V 0 0 0 0 0 0 1 0 1 0 0 0 V 0 0 0 1 0 0 0 0 0 0 0 1 VI 0 1 0 3 4 0 3 0 2 0 0 0 VI 0 1 0 0 0 0 0 0 0 1 0 0 Rata-rata 0.00 0.210 V 0. Bobot Total Feses Mencit Ulangan II III IV 0.261 0.954 Universitas Sumatera Utara .67 0.50 0.50 0.876 3.780 0.230 Ulangan II III IV 0.33 0.00 Rata-rata 0.500 VI 0.17 0.802 2.220 VI 0.00 0.17 0.50 0.17 0.291 Bobot (g) V 0.33 1.701 V 0.882 Perlakuan Kelompok I Kontrol negatif Kelompok II Loperamid Kelompok III EEBS 1g/kg BB Kelompok IV EEBS 2 g/kg BB Kelompok V EEBS 2.860 V 0.500 Ulangan II III IV 0.900 0.590 0.230 VI 1.570 0.83 2.00 0.760 1.182 0.00 0.212 5.17 c.991 1.670 Ulangan II III IV 0.120 V 0.190 1.Lanjutan Lampiran 9 Kelompok IV Waktu EEBS 2 g/kg BB (menit ke) 30 60 90 120 150 180 210 240 270 300 330 360 Kelompok V Waktu EEBS 2.620 VI 0.021 I 0.17 0.17 0.50 0.681 0.5 g/kg BB I 1.111 VI 0.83 0.50 0.510 I 0.291 I 0.530 I 0.811 0.

0 5-10 5-10 10. Mencit (20-30 g) Tikus (200 g) Hamster (50 g) Marmot (300 g) Kelinci (2.0 5-10 5-10 10-20 i.m.o.25 0.5-1. 1.0 2. 1974) Volume maksimum (ml) sesuai jalur pemberian Jenis hewan uji i.0 50.1 0. 0.c.0 100. 1.5 10.0 p.p.v.5 1.5 kg) Kucing (3 kg) Anjing (5 kg) 0. Volume Maksimum Larutan Sediaan Uji yang Dapat diberikan Pada Hewan Uji (Ritachel.0 5.0 5.5 1.0 Universitas Sumatera Utara .0 2-5 1-2 2-5 10-20 10-20 20-50 s.0 2-5 2.1 0.05 0.0 i. 0.0 20.Lampiran 10.5 5.

25 1.016 0.1 1.1 3.57 0.2 6.06 0.0 0.5 1.44 0.52 0.9 mg = 19.08 0.5 g/kg BB dosis optimal ekstrak etanol buah sawo pada mencit 50 mg/20 g BB faktor konversi dosis mencit ke manusia = 387.07 Kera 4kg 64.0 0. Perhitungan Konversi Dosis Konversi dosis antara jenis hewan dengan manusia (Laurance dan bacharach.2 5.0 0.2kg Kera 4kg Anjing 12kg Manusia 70kg 1.0 0.2 2.32 Manusia 70kg 387.25 1.07 mg/kg BB manusia rendemen = berat simplisia berat basah = 280 g 1100 g X 100% X 100% = 24.0 1.008 0.0 31.1 9. 1964).395 g/70 kg BB manusia = 277. Perhitungan konversi dosis ekstrak etanol buah sawo dari mencit ke manusia Dosis yang digunakan dalam penelitian adalah : 20 mg/ 20 g BB atau sama dengan 1 g/kg BB 40 mg/20 g BB atau sama dengan 2 g/kg BB 50 mg/20 g BB atau sama dengan 2.9 56.22 0.04 0.031 Kelinci 1. Mencit 20g Mencit 20g Tikus 200g Marmot 400g Kelinci 1.10 0.9 1.0026 Tikus 200g 7.0 0.74 1.16 Anjing 12kg 124.45 % Universitas Sumatera Utara .9 dosis manusia dosis manusia = dosis mencit x faktor konversi = 50 mg x 387.8 10.4 1.5kg 27.42 0.0 0.19 0.5 14.8 3.Lampiran 11.0 a.2 4.9 2.25 0.11 0.018 Marmt 400g 12.14 0.2 17.

89 g buah sawo muda pada manusia.lanjutan Lampiran 11 rendemen = berat ekstrak berat simplisia X 100% = 151. Perhitungan konversi loperamid dari manusia ke mencit Dosis manusia = 2 – 8 mg/ hari tergantung beratnya diare dan respon penderita.26 mg – 1.56% maka pemakaian 2.0026) = 0.0208 mg/ hari dan tidak lebih dari 0. b.04 mg/kg BB dan tidak lebih dari 2.132 g 200 g X 100% = 75. Dosis tidak boleh lebih dari 16 mg/hari (Suputra.66 g simplisia atau 100.08 mg/kg BB. Universitas Sumatera Utara .0416 mg untuk 20 g mencit atau sama dengan 0.0052 mg – 0.5 g/kg BB mencit setara dengan 25. dosis mencit = dosis manusia X faktor konversi (0. 2002).

2 g/kg BB dan 2.5 g/ kg BB dan Loperamid 2 mg/kg BB (tablet Imodium®) a. 2.5 g/1000 x 30 = 0.03 g = 30 mg konsentrasi ekstrak volume pemberian = 300 mg/5ml = 30 mg/ 300 x 5 ml = 0. Perhitungan Volume Pemberian EEBS dosis 1 g. EEBS dosis 1 g/kg BB berat badan mencit = 30 g dosis pemberian = 1 /1000 x 30 = 0. 2 g. EEBS dosis 2. Dosis Estrak etanol buah sawo yang digunakan dalam penelitian adalah : 1 g/kg BB .5 ml 2.06 g = 60 mg konsentrasi ekstrak volume pemberian = 600 mg/5 ml = 60 mg/ 600 x 5 ml = 0.5 ml 3.075 g = 75 mg Universitas Sumatera Utara .Lampiran 12.5 g/Kg BB berat badan mencit dosis pemberian = 30 g = 2. EEBS dosis 2 g/kg BB berat badan mencit dosis pemberian = 30 g = 2 g/1000 x 30 = 0.5 g/kg BB Volume pemberiannya adalah: 1.

lanjutan Lampiran 12 konsentrasi ekstrak = 750 mg/5 ml volume pemberian = 75 mg/ 750 x 5 ml = 0.3 ml X 30 g = 0.06 mg 2. Dosis Loperamid yang digunakan adalah 2 mg/kg BB .5 ml b.06 mg Universitas Sumatera Utara . maka volume pemberiannya adalah dosis untuk 30 g mencit = konsentrasi Loperamid = volume pemberian = 2 mg 1000 2 mg/10 ml 0.00 mg X 10 ml = 0.

000 Homogeneous Subsets jumlah Subset for alpha = 0.833 156. Universitas Sumatera Utara .3333 1.967 df 4 25 29 Mean Square 31. .133 29.783 1.5 g/kg BB EEBS 2 g/kg BB EEBS 1 g/kg BB kontrol negatif Sig. Uses Harmonic Mean Sample Size = 6. Oneway ANOVA Jumlah Sum of Squares Between Groups Within Groups Total 127.0000 4.Lampiran13 .5000 .000 . Hasil Uji Statistik Jumlah Mencit Diare ONEWAY jumlah BY perlakuan /MISSING ANALYSIS /POSTHOC=DUNCAN LSD ALPHA(0.5000 5.05 Perlakuan Duncan a N 6 6 6 6 6 1 .05). a.5000 2 Loperamid EEBS 2. 4.055 Means for groups in homogeneous subsets are displayed.634 Sig.193 F 26.000.

467 51.833 5. Oneway ANOVA Sum of Squares menit 30 Between Groups Within Groups Total menit 60 Between Groups Within Groups Total menit 90 Between Groups Within Groups Total menit 120 Between Groups Within Groups Total menit 150 Between Groups Within Groups Total menit 180 Between Groups Within Groups Total menit 210 Between Groups Within Groups Total menit 240 Between Groups Within Groups Total menit 270 Between Groups Within Groups Total menit 300 Between Groups Within Groups Total menit 330 Between Groups Within Groups Total menit 360 Between Groups Within Groups Total .611 81.667 5.266 2.043 11.000 92.694 .609 49.sav'.220 68.965 .709 104.533 4.416 1.042 102.667 6.973 3.012 1.200 58. .063 71.882 .833 60.386 .902 1.338 1.250 .026 2.690 Sig.982 .073 1.193 F .950 2.520 .05).133 .984 3.633 47.800 8.553 13.603 Mean Square .868 2.888 7.772 53.467 1.167 .003 31.000 7.351 34.333 75.682 .000 70.300 26.760 .533 1.652 40.101 .117 2.200 24.167 10.050 2.208 100.800 63.000 6.322 4.167 Df 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 .Lanjutan Lampiran 13 Konsistensi Feses GET FILE='C:\Users\User\Documents\konsistensi data.005 17.367 .444 .200 44. ONEWAY menit30 menit60 menit90 menit120 menit150 menit180 menit210 menit240 menit270 menit300 menit330 menit360 BY perlakuan /MISSING ANALYSIS /POSTHOC=DUNCAN LSD ALPHA(0.842 7.050 .133 49.774 .606 Universitas Sumatera Utara .311 .021 .001 8.240 .408 1.242 33.960 17.367 .611 6.000 9.760 .

menit 90 Subset for alpha = 0.1667 . Means for groups in homogeneous subsets are displayed.05 Perlakuan Duncan a N 6 6 6 6 6 1 . a.3333 .000.3333 .3333 .5000 .302 loperamid EEBS 2 g/kg bb EEBS 1 g/kg bb Kontrol negatif EEBS 2.9450 1. Uses Harmonic Mean Sample Size = 6.05 Perlakuan Duncan a N 6 6 6 6 6 1 .1667 .0000 .1667 .Lanjutan Lampiran 13 Homogeneous Subsets menit 30 Subset for alpha = 0.251 Kontrol negatif EEBS 2 g/kg bb loperamid EEBS 1 g/kg bb EEBS 2.3333 . a.000. Means for groups in homogeneous subsets are displayed. Means for groups in homogeneous subsets are displayed.0000 . Universitas Sumatera Utara .5000 .000. Uses Harmonic Mean Sample Size = 6.5 g/kg bb Sig.165 loperamid EEBS 2. a.5 g/kg bb Sig.5 g/kg bb EEBS 2 g/kg bb Kontrol negatif EEBS 1 g/kg bb Sig.05 Perlakuan Duncan a N 6 6 6 6 6 1 . menit 60 Subset for alpha = 0. Uses Harmonic Mean Sample Size = 6.1667 .0833 .5000 .

Uses Harmonic Mean Sample Size = 6.6667 3. Uses Harmonic Mean Sample Size = 6.1667 4.5 g/kg bb loperamid EEBS 2 g/kg bb EEBS 1 g/kg bb Kontrol negatif Sig.5 g/kg bb loperamid Kontrol negatif EEBS 2 g/kg bb EEBS 1 g/kg bb Sig. Universitas Sumatera Utara .184 Means for groups in homogeneous subsets are displayed.05 Perlakuan Duncan a N 6 6 6 6 6 1 .0283 . menit 150 Subset for alpha = 0.5000 2 3 EEBS 2.222 . 1.5833 .385 .0000 3.8333 2 3 EEBS 2.353 .1667 .0000 .0000 .05 Perlakuan Duncan a N 6 6 6 6 6 1 .153 Means for groups in homogeneous subsets are displayed.9167 2 loperamid EEBS 2.0000 2.5000 1.0000 2.100 Means for groups in homogeneous subsets are displayed.000.9167 3. a. menit 180 Subset for alpha = 0. a.2500 1. a.5 g/kg bb EEBS 2 g/kg bb EEBS 1 g/kg bb Kontrol negatif Sig. Uses Harmonic Mean Sample Size = 6.000.1667 3.561 . 2.Lanjutan Lampiran 13 menit 120 Subset for alpha = 0.058 . .05 Perlakuan Duncan a N 6 6 6 6 6 1 .1667 .8333 2.000.0000 2.

05 Perlakuan Duncan a N 6 6 6 6 6 1 .159 Means for groups in homogeneous subsets are displayed. menit 270 Subset for alpha = 0.354 Means for groups in homogeneous subsets are displayed.6667 1.5000 . a. menit 240 Subset for alpha = 0.5000 2 EEBS 2. Uses Harmonic Mean Sample Size = 6.5000 .0000 .0000 .5000 3.05 Perlakuan Duncan a N 6 6 6 6 6 1 .6667 .000.000. Uses Harmonic Mean Sample Size = 6.5 g/kg bb loperamid EEBS 1 g/kg bb Kontrol negatif EEBS 2 g/kg bb Sig.155 EEBS 2.3333 . Uses Harmonic Mean Sample Size = 6.Lanjutan Lampiran 13 menit 210 Subset for alpha = 0. 2.1667 1.05 Perlakuan Duncan a N 6 6 6 6 6 1 .000.7783 2.113 .5 g/kg bb loperamid Kontrol negatif EEBS 1 g/kg bb EEBS 2 g/kg bb Sig. Means for groups in homogeneous subsets are displayed. Universitas Sumatera Utara .1667 .0000 2. 2.5833 3.7500 .5 g/kg bb EEBS 1 g/kg bb Kontrol negatif Sig. a.0000 2.331 . a.8333 2 EEBS 2 g/kg bb loperamid EEBS 2.

1667 . menit 330 Subset for alpha = 0.0000 . a.461 1.0000 .221 loperamid EEBS 2.6667 .1667 .05 Perlakuan Duncan a N 6 6 6 6 6 1 .6667 .Lanjutan Lampiran 13 menit 300 Subset for alpha = 0.5000 1.5 g/kg bb Kontrol negatif Sig.3333 . Uses Harmonic Mean Sample Size = 6. Means for groups in homogeneous subsets are displayed.3333 . Uses Harmonic Mean Sample Size = 6.000 Means for groups in homogeneous subsets are displayed. menit 360 Subset for alpha = 0. Means for groups in homogeneous subsets are displayed.05 Perlakuan Duncan a N 6 6 6 6 6 1 .5 g/kg bb Kontrol negatif Sig.05 Perlakuan Duncan a N 6 6 6 6 6 1 . Universitas Sumatera Utara .000. Uses Harmonic Mean Sample Size = 6. a.6667 2 EEBS 2 g/kg bb loperamid EEBS 1 g/kg bb EEBS 2. a.0000 .212 EEBS 1 g/kg bb EEBS 2 g/kg bb Loperamid EEBS 2.1667 .1667 .5000 .5 g/kg bb EEBS 2 g/kg bb Kontrol negatif EEBS 1 g/kg bb Sig.3333 1. 2.000.000.

242 39.833 60.500 85.333 75.839 .603 Mean Square .050 2.533 4.133 49.860 18.467 51.311 .253 .543 .033 68.014 11.021 .005 16.491 1.667 8.667 6.200 58.533 1.750 10.300 26.602 3.944 31.333 56.05).042 102.833 5.704 4.973 3.367 .395 .193 F .117 .611 72.118 29.219 .167 10.542 108.322 4.026 2.240 .993 40.951 65.042 104.682 .250 . .167 df 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 .167 .467 1.481 .998 1.988 2.000 7.967 44.611 6.018 9.606 Universitas Sumatera Utara . Oneway ANOVA Sum of Squares menit 30 Between Groups Within Groups Total menit 60 Between Groups Within Groups Total menit 90 Between Groups Within Groups Total menit 120 Between Groups Within Groups Total menit 150 Between Groups Within Groups Total menit 180 Between Groups Within Groups Total menit 210 Between Groups Within Groups Total menit 240 Between Groups Within Groups Total menit 270 Between Groups Within Groups Total menit 300 Between Groups Within Groups Total menit 330 Between Groups Within Groups Total menit 360 Between Groups Within Groups Total .740 .667 6.008 2.694 .008 1.575 4.sav'.333 60.650 .367 .760 .690 Sig.117 2.547 .174 24.Lanjutan Lampiran 13 Frekuensi Defekasi GET FILE='C:\Users\User\Documents\frekuensi defekasi.073 1.000 6.083 2. ONEWAY menit30 menit60 menit90 menit120 menit150 menit180 menit210 menit240 menit270 menit300 menit330 menit360 BY perlakuan /MISSING ANALYSIS /POSTHOC=DUNCAN LSD ALPHA(0.742 3.133 .467 21.576 64.416 1.965 42.000 9.

Means for groups in homogeneous subsets are displayed. Means for groups in homogeneous subsets are displayed.05 Perlakuan Duncan a N 6 6 6 6 6 1 . Uses Harmonic Mean Sample Size = 6.3333 .Lanjutan Lampiran 13 Homogeneous Subsets menit 30 Subset for alpha = 0.9450 1.0000 .5 g/kg bb Sig.5 g/kg bb Sig. menit 60 Subset for alpha = 0.0000 .302 loperamid EEBS 2 g/kg bb EEBS 1 g/kg bb kontrol negatif EEBS 2.1667 .132 loperamid EEBS 2 g/kg bb EEBS 2.5 g/kg bb kontrol negatif EEBS 1 g/kg bb Sig.000. a.05 Perlakuan Duncan a N 6 6 6 6 6 1 . menit 90 Subset for alpha = 0.000.5000 . Means for groups in homogeneous subsets are displayed.3333 . Uses Harmonic Mean Sample Size = 6.000.5000 .3333 .3333 . Universitas Sumatera Utara .0000 .1667 .1667 . a.251 kontrol negatif EEBS 2 g/kg bb loperamid EEBS 1 g/kg bb EEBS 2.0000 . Uses Harmonic Mean Sample Size = 6.05 Perlakuan Duncan a N 6 6 6 6 6 1 . a.1667 .

05 Perlakuan Duncan a N 6 6 6 6 6 1 .000.0000 2.0000 .8333 2 3 EEBS 2. menit 150 Subset for alpha = 0.0283 . a.5000 1.5 g/kg bb loperamid kontrol negatif EEBS 2 g/kg bb EEBS 1 g/kg bb Sig. 1. Uses Harmonic Mean Sample Size = 6.5833 .5000 2 3 EEBS 2.0000 .0000 2.5 g/kg bb EEBS 2 g/kg bb EEBS 1 g/kg bb kontrol negatif Sig. a.5 g/kg bb loperamid EEBS 2 g/kg bb EEBS 1 g/kg bb kontrol negatif Sig.222 .000.5833 3.000.05 Perlakuan Duncan a N 6 6 6 6 6 1 .0000 3.100 Means for groups in homogeneous subsets are displayed. menit 180 Subset for alpha = 0. .096 . a.353 .6667 2.1667 .000 Means for groups in homogeneous subsets are displayed.539 .05 Perlakuan Duncan a N 6 6 6 6 6 1 .067 Means for groups in homogeneous subsets are displayed.0000 2.8333 4.8333 2.2500 1. Uses Harmonic Mean Sample Size = 6.Lanjutan Lampiran 13 menit 120 Subset for alpha = 0. Universitas Sumatera Utara . Uses Harmonic Mean Sample Size = 6.758 1.1667 . 2.5833 2 loperamid EEBS 2.

5 g/kg bb loperamid EEBS 1 g/kg bb EEBS 2 g/kg bb kontrol negatif Sig. .140 Means for groups in homogeneous subsets are displayed. Uses Harmonic Mean Sample Size = 6.0000 3. a.5833 1.3333 2.3333 2. a.7783 2.05 Perlakuan Duncan a N 6 6 6 6 6 1 . Uses Harmonic Mean Sample Size = 6.05 Perlakuan Duncan a N 6 6 6 6 6 1 . Uses Harmonic Mean Sample Size = 6.5000 . Means for groups in homogeneous subsets are displayed.095 .3333 . Universitas Sumatera Utara .0000 .058 Means for groups in homogeneous subsets are displayed.05 Perlakuan Duncan a N 6 6 6 6 6 1 .7500 .8333 1.3333 2 3 loperamid EEBS 2.6667 .5 g/kg bb loperamid kontrol negatif EEBS 1 g/kg bb EEBS 2 g/kg bb Sig.1667 3.1667 .0000 2 EEBS 2. menit 240 Subset for alpha = 0.6667 . a.000.5 g/kg bb EEBS 2 g/kg bb EEBS 1 g/kg bb kontrol negatif Sig.1667 .8333 1.240 EEBS 2. 2. menit 270 Subset for alpha = 0.216 .260 .Lanjutan Lampiran 13 menit 210 Subset for alpha = 0.000.5833 3.000.8333 1.

6667 .5 g/kg bb kontrol negatif Sig. a.221 loperamid EEBS 2. menit 360 Subset for alpha = 0.0000 .05 Perlakuan Duncan a N 6 6 6 6 6 1 . Uses Harmonic Mean Sample Size = 6.05 Perlakuan Duncan a N 6 6 6 6 6 1 .1667 .000.6667 2 EEBS 2 g/kg bb loperamid EEBS 1 g/kg bb EEBS 2.6667 . Universitas Sumatera Utara . a.05 Perlakuan Duncan a N 6 6 6 6 6 1 .0000 .5000 . Means for groups in homogeneous subsets are displayed.461 1.212 EEBS 1 g/kg bb EEBS 2 g/kg bb loperamid EEBS 2.000.1667 .3333 .5 g/kg bb kontrol negatif Sig.5 g/kg bb EEBS 2 g/kg bb kontrol negatif EEBS 1 g/kg bb Sig. a.Lanjutan Lampiran 13 menit 300 Subset for alpha = 0.1667 . 2. Means for groups in homogeneous subsets are displayed.3333 1.0000 .000. Uses Harmonic Mean Sample Size = 6. menit 330 Subset for alpha = 0. Uses Harmonic Mean Sample Size = 6.5000 1.3333 .000 Means for groups in homogeneous subsets are displayed.1667 .

.3257 .625 N 6 6 6 6 6 1 .816 2.4995 2 3 Loperamid EEBS 2.5 g/kg bb EEBS 2 g/kg bb EEBS 1 g/kg bb kontrol negatif Sig.000.4995 .033 F 13.808 . Oneway ANOVA Bobot Sum of Squares Between Groups Within Groups Total Homogeneous Subsets Bobot Subset for alpha = 0.000 Means for groups in homogeneous subsets are displayed.845 Sig.210 1.05).Lanjutan Lampiran 13 Bobot Total Feses ONEWAY bobot BY perlakuan /MISSING ANALYSIS /POSTHOC=DUNCAN LSD ALPHA(0. a. Universitas Sumatera Utara .3127 . Uses Harmonic Mean Sample Size = 6.6337 . .000 1.102 .9803 .452 .05 Perlakuan Duncan a df 4 25 29 Mean Square .

000 .750 .000 .133 .004 .167 1.450 .190 .333 2. Mean Square .800 4.833 .385 . ANOVA Sum of Squares menit 30 Between Groups Within Groups Total menit 60 Between Groups Within Groups Total menit 90 Between Groups Within Groups Total menit 120 Between Groups Within Groups Total menit 150 Between Groups Within Groups Total menit 180 Between Groups Within Groups Total menit 210 Between Groups Within Groups Total menit 240 Between Groups Within Groups Total menit 270 Between Groups Within Groups Total menit 300 Between Groups Within Groups Total menit 330 Between Groups Within Groups Total menit 360 Between Groups Within Groups Total .140 1.190 .135 .875 .140 1.000 .200 .187 3.000 .000 .467 2.800 3.107 1.867 4.147 6.016 .05).750 .426 .016 .167 .467 2.Lanjutan Lampiran 13 Mencit yang Mengalami Diare ONEWAY menit30 menit60 menit90 menit120 menit150 menit180 menit210 menit240 menit270 menit300 menit330 menit360 BY perlakuan /MISSING ANALYSIS /POSTHOC=DUNCAN LSD ALPHA(0.750 .033 .000 4.800 2.200 .000 .000 F .087 15.120 3.147 5.967 df 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 4 25 29 .000 .000 .667 3.000 .146 . Universitas Sumatera Utara .339 .000 .667 7.477 .114 .950 . .700 .667 6.000 3.001 1. Sig.800 .800 3.333 7.333 .467 3.339 .000 .010 .167 .033 1.500 4.667 5.667 3.500 4. .500 3.173 4.167 7.717 .167 .667 3.667 7.

a.5 g/kg bb EEBS 2 g/kg bb EEBS 1 g/kg bb kontrol negatif Sig.6667 .144 .0000 .8333 1. a.074 Means for groups in homogeneous subsets are displayed.5000 .05 Perlakuan Duncan a N 6 6 6 6 6 1 .3333 .000 .3333 .3333 2 3 loperamid EEBS 2.125 loperamid EEBS 2 g/kg bb EEBS 2. Uses Harmonic Mean Sample Size = 6.8333 .0000 1. Means for groups in homogeneous subsets are displayed. .5 g/kg bb EEBS 2 g/kg bb EEBS 1 g/kg bb kontrol negatif Sig. Uses Harmonic Mean Sample Size = 6. menit 150 Subset for alpha = 0.000.05 Perlakuan Duncan a N 6 6 6 6 6 1 . a. Uses Harmonic Mean Sample Size = 6. Universitas Sumatera Utara .0000 .Lanjutan Lampiran 13 Homogeneous Subsets menit 90 Subset for alpha = 0.458 Means for groups in homogeneous subsets are displayed.0000 .05 Perlakuan Duncan a N 6 6 6 6 6 1 .5 g/kg bb kontrol negatif EEBS 1 g/kg bb Sig.0000 .000. .0000 .5000 .0000 . menit 120 Subset for alpha = 0.000.0000 2 loperamid EEBS 2.3333 .166 .

5 g/kg bb EEBS 1 g/kg bb kontrol negatif EEBS 2 g/kg bb Sig.202 .1667 .05 Perlakuan Duncan a N 6 6 6 6 6 1 .05 Perlakuan Duncan a N 6 6 6 6 6 1 .3333 .1667 .000.1667 .000.059 Sig. Uses Harmonic Mean Sample Size = 6. a. Means for groups in homogeneous subsets are displayed.8333 .1667 2 .0000 .0000 . Universitas Sumatera Utara . menit 210 Subset for alpha = 0.1667 .535 Means for groups in homogeneous subsets are displayed.Lanjutan Lampiran 13 menit 180 Subset for alpha = 0.144 . a.6667 .6667 2 loperamid EEBS 2.5 g/kg bb EEBS 2 g/kg bb EEBS 1 g/kg bb kontrol negatif .5000 .000.3333 .059 3 loperamid EEBS 2.144 .1667 .458 .5 g/kg bb loperamid kontrol negatif EEBS 2 g/kg bb EEBS 1 g/kg bb Sig. a. Uses Harmonic Mean Sample Size = 6.3333 2 .458 Means for groups in homogeneous subsets are displayed.8333 .05 Perlakuan Duncan a N 6 6 6 6 6 1 .0000 .6667 1. . .8333 .6667 . menit 240 Subset for alpha = 0.6667 . Uses Harmonic Mean Sample Size = 6.068 .6667 .5000 3 4 EEBS 2.

000. Means for groups in homogeneous subsets are displayed. a. N 6 6 6 6 6 .3333 .3333 . Uses Harmonic Mean Sample Size = 6.05 Perlakuan Duncana loperamid EEBS 2 g/kg bb EEBS 1 g/kg bb EEBS 2.179 loperamid EEBS 2. menit 300 Subset for alpha = 0.0000 .0000 .3333 .0000 .0000 .05 Perlakuan Duncana loperamid EEBS 2.455 1 . Uses Harmonic Mean Sample Size = 6.6667 1.000.0000 . a.179 menit 330 Subset for alpha = 0. N 6 6 6 6 6 1 .0000 . Uses Harmonic Mean Sample Size = 6.Lanjutan Lampiran 13 menit 270 Subset for alpha = 0.5 g/kg bb EEBS 1 g/kg bb kontrol negatif EEBS 2 g/kg bb Sig.1667 . Means for groups in homogeneous subsets are displayed.1667 .1667 .5 g/kg bb EEBS 2 g/kg bb kontrol negatif EEBS 1 g/kg bb Sig.5 g/kg bb kontrol negatif Sig.000.1667 .000 2 Universitas Sumatera Utara .05 Perlakuan Duncan a N 6 6 6 6 6 1 . Means for groups in homogeneous subsets are displayed.3333 . a.

0000 . Uses Harmonic Mean Sample Size = 6.05 Perlakuan Duncana loperamid EEBS 1 g/kg bb EEBS 2 g/kg bb EEBS 2.Lanjutan Lampiran 13 menit 360 Subset for alpha = 0.169 ONEWAY menit30 menit60 menit90 menit120 menit150 menit180 menit210 menit240 menit270 menit300 menit330 menit360 BY perlakuan /MISSING ANALYSIS /POSTHOC=DUNCAN LSD ALPHA(0. Means for groups in homogeneous subsets are displayed. Universitas Sumatera Utara .0000 . N 6 6 6 6 6 1 .5 g/kg bb kontrol negatif Sig.000.0000 . a.1667 .0000 .05).