# Name: Diffusion, Osmosis & Water Potential Worksheet #2 Water potential = Osmotic (solute) potential + Pressure potential Ψw = Ψs + Ψp ψs = -iCRT i = Ionization

constant (e.g. 1.0 for sucrose [no ionization]); assume that i = 1 unless otherwise indicated C = Molar concentration R = Pressure constant (R = 0.0831 liter bars/mole oK) T = Temperature oK (273 + oC of solution) Calculation Question: 1. Determine the water potential of a cell if Ψp = 0.3 MPa and Ψs = -0.5 MPa. 2. Calculate the pressure in a cell if Ψw = -0.1 and Ψs = -0.2. 3. Calculate the osmotic potential of a cell (Ψs) is Ψw = -0.1 and Ψp = 0.8 Osmometer Question: The diagram represents a simple osmometer. The membrane is freely permeable to water but not solute (sucrose). Inside the membrane is a solution of 0.1 M sucrose. In the beaker is distlled water. For the questions below, please circle the best answer. 1. There will be a net movement of water ________ the membrane sac. a. into b. out of 2. The water level in the tube will: a. fall b. rise c. not change 3. The pressure in the sac will: a. decrease b. increase change c. not

4. On the back of this worksheet, sketch the shape of the line/curve plotting osmotic potential in the sac vs. time 5. On the back of this worksheet, sketch the shape of the line/curve plotting pressure in the sac vs. time.