BRLONF 2000558 W228 Fim SEIS. 1004-7220(2006)04.0075.08 Journal of Medical Biomechanics, Vol 22. No.1, March 2007 15 FP 3S SES A RB BBA HT (eR HE BE be Be PRB. BO, TAR, HE CHUA SLER ER. Le 200002) RB: FLA 7 A— Hes AGREE Fama imestnal ubmacos,SIS)SEBLIRFIL. AE HORA ‘SeS1S oR AOS PAE MACE, IAT ESTA TR ik, AAT EAPEDIRSIE Ou. RIE 110.3MPa NE FOOSE) ARATE. R= RAR (6.01 + 1.43).(6.96 + 093)40(12.94 + 2.03)MPa, (R—=BEILIISG SIS AAR LAT RAA, URIS I TIRED ESCM SIS ITAA HINTS. Ia OWL a-aP ASIST TH, ET 1 ERESISHO DD EHO GL RE ARES 2 8 HEISE GER HI AHPC SS REPL SMR: ALLA, NAAURIE: ROR. ALLE PHAGE: RIRO8, O19 ERE Se ees TLR Uniaxial tensile mechanical properties of porcine SIS following different cross-linking treatments NIU Wen-xin, LENG Ye, DING Zu-quan, GAO Yan-gin Tongli University, Shanghal 200092, China) Abstract: Objective As a new graft biomaterial for tissue engineering, smalntestinal submucos (Schoo! of Life Science & Technology Engineering, (SIS) has an excelent biomechanical property. This study aims o investigate an idea method for crossinking porcine smabintestinal submucosa, Methods Stabilizing porcine SIS by Glutaraldehyde(GA) and photoiing samples from the same animal with methylene blu, then testing them together with orginal samples by matoral test machine. Data were obtained and analyzed, including utimate tense tess (Gn) breaking strain (2), and modulus of elastcty(E) under 0.3MPa stress. Results The utimate tensile stress of three groups are respectively 6,011.43. 6.9620.93 and 12.0442.03MPa. ‘Samples following GA treatment improve correspondingly, but stifness ofthe issue is increased. Intensity of samples following photofixing with methyfne blue improve slightly, and lxity improve. Conclusion This study stabilized porcine SIS by GA and methylene bie forthe fst ime, and through comparing their biomechanical properties of uniaxial {ensity, it was tested that the method of photooidaton shall be developed as a valid treatment to crosslink SS. Key words: Tissve engineering; Smal-ntestinal submucosa: Gluaraldahyde; Photooxidation “ls S8I0L F Fa (small-intestinal submucosa, SIS) RISA ALTAR. APIA FERAL IRE, SURE UiTE, AIRE POR Rt Bi SIS CEsRER TPES SE TACT ENO A. A FED Jy EET A Bh EL, MEM, 2006-06-19, WEAN, 2006-08-07 YSIS MPRA OT LAE EIT LSM, Sacks(1999)P11A 9, SIS 38 —FABEA TW A "I PE REO A SE ‘SUS JB J BE A EE BI i SAA 80 AR SERB. FRALIONO-). Be ME. ERAT I. Met hy AGEN MA RIA. PPE, Tele (02165082586; Ema lengye@ mall tngieducnBReane 20783A m2 RIM 6 Joumal of Medical Blomechancs, Vol HEAR, RUEWE SIS AP ARIOT AT BA HAT LE EAA. CeLSPs, XY SIS HSER T FRI ANAE DELO XAL. ULB LEAT SIS i FAD EM, AI SIS HO TTB FE ASR AS PLB ATA 1/7 BA ROM EAD EME, TEOLK SIS MERU SHE SA, RE A A MAE HH, HEM TART IR, 2B MOET REA, SA SIS MEDD EHH. TERA RE OS OPAL ARE, AE eR TARAS, FEAR DEM, CRAMER AEH, SCARS EAL PE He MUI SIM SIS MAT Tb, SEES Ts BAER SIS HE A A Uy HR IEE. 1 MASA 11 ate 1.1.1 FURR HE) ACA RRR 2G, YET ARCATA TE. BL BCP —-B. ARAL IEL, 2ORMRM, FE #3 LY 100 mm ABR. FFA TIS RULE. RE 2B ME, HE EE tok eA. 11.2 RO MECARAPENIC A) 481.1) Bei RTEREEE Soe NaCl Mbithi ah kt Sea ARETE HE 0.625% MRRP ALE — A, I (aL A 1.1.3 Je LHEARIRADEERRAE Ail A) ¥9(1.1.1) EBERT A Ko HR ce RAORLP, AA 0.1% SEAR HAH PBS ‘898(pHL:7.6,0sm:320mosm), “Fi 4 h fF 200W FURATSE 25°C FEET 60h. SHI] AL a — Wes ARIE RA LP ERENT AE 1.2 SLT HS ALURPOOTR 1 AL, AIM CS8-44010 HR ‘BUC ARAM ALFIE) AT LL ae MO EET AH Wit, RAGA. Watire SIS MARE RTE. INL 20 mm AYALA. EL 8 mm/min IEE ATA, PRET BEA 8k, BMRA META RAE 30% (RAEN 4.5. N, ARREARS AN FR ARLE He ERY 5.5 N). TE SIR A 22 No.1, March 2007 8 mm/min, ASA, HILAL AN A B. THAT BRATS RRR UAB UL, SRL EPMA ST ER LH ARR JLT BYU AE RR APPR. EZ AUR LITO ALE AMAA LE SME AAR, FR A EH te 1. Tl, Sait a 10, 13 10 FF Vai net Me chro waka a vareriAtes 0 kms RIP 6 ESLER ET RUSH) Fig. 1 uniaxial tensile test on porcine SIS. (a) for original ‘sample () for GAeed sample; (cfr sample folowing phottred th methylene bive,) 13 SHR CEN EY EE REF PLEO ORF Green KEE e=(2-)/2 Ml Bulerian Ao = 72. MABURRR BEAL IRS ER A A DUAR RYE on (HA BL) AT ARTE Green WAH En HERR IME. BH, VUE 2 O.3MPa SPREE 10 PRELIM HATA 0.296, mam) Br 95 1 A RE SR EE Ee SSRN J NTT MD AHL FE RR TE MERE, FF AT RUT. AUB es HAR, A Dunnert-r OH, EIT fF SPSS12.0. 2 &R SUPRA MOL PS AEA BIRR 1 AT ICUS RRL, MRR BALAI 7 PE ATER. Sm Jy se eR MR 2 EA. AL GBA TMM HR MIN HER RE HL 3. AERA Mie Tn, BAR BOS ACEI.) Be AIDA EF NAR 8 FS ETE EK, TAMER ABM. SMM Ss DRENESE Em AE EAE EM WR LE IR AErs NU Westin, oa: Uniaxial ‘ tn t t ; o o B2 MSS mMRMEDE EARS hanial properties of porcine SIS following ditrent crosstinking treatments _ Or 02 03 08,0306 0 00s OT MOQ RNR Ke MRR MEIN 'L va gE os © ATLA) b REAR ARAREA CL A; IP AERC MERIAL) Fig. 2. Stross-strain curve of uniaxial tensile test on porcine SIS _((a) or eriginal group; (b) for GA-tted group) for group {allowing photfixed with mathylane ble.) RRR SIS BHD RATELEas) mechanical property of pot ents. 1 after aitterent a Sve GHnkEK week Tike Pa) is Totes. 12944203" 0.34930.117" 8.2284.74 6.964093 _0.25940.070_12.3245.39 So MRA TILA 7P<005, “POOL BERL AKARIE: TALC, 1 AL TEESE (P=0.000), TL HIG BYE IE(P= 0.209). Hi ie y CALE ALA AB HEHE IE(P=0.032), UT EBB YEIEH 20.545). HE ERUR. 45 1 (PLS FE. Ate ECA HL P=0.343, U1 HL P=0.093). 3 TARO RAD SETH SIS KB ABE RSE MOREAU UII. SIS PTE AE LA AT HR RAN ATL, BTL HE SIS AB Hy 7 FTA RARE, MEE. BY HASAN ERE. RHE Sek RLM ANH. CART MBt mee. Wi. ORT. BERR. RATER. AIR. M4 STITH AANA, LEH) PSST SLA AL AHS OTR. (ELAR, eR ESHER, UNAS CDRH EHLERS RAT viii SAL. MR PE UE IOOT AEE SMO, EAT ALE RTE, ANIME Shs MAAC AUMEHE. BARA TATA AY RAC ERIE "DR AU NH RES BIAS EEA REM, OTS BEC BSF PRAM AE INE Lo MERAH BA ARERR, AB ARE FAT AER SULIT RIS, HE RCE DR BRA. BEART RET mT HE eH RE, RUIN FE, RL FSR A BC PERE AR MLO, SA RN IR AR — Bm. De EE EAR EH, APACE, 01 | A EL RU A aot, AeSEWRH AE HREM Schitt WALA FLA Bee AE BO, 4 RAE AUREL SPR wT LAE EET BE. OUI RG, RONEMA ERAAE. SE ICEL LAL EIB dye-mediated photooxidation, DMP) ‘IRR. 4 DMP TIERRA, CEM REA LB EATIMS RIA WBE, BOR eM e L BSR ILEANA JL ERIE On AR BA sn, DM CBR ACS ok 1 Se HVS) — SUSUR. BUA TIRE BOR. BRERA LAP 3 EB a EAS iA SRA J A I, SS SUITOR TL RRMA CAE. BRAD ZR LH O.3MPa Hi EAR 0 A EE RS RE, ARORA La FA FE