ATP Is the Universal Currency of Free Energy in Biological Systems
ATP is an energy-rich molecule because its triphosphate unit contains two
phosphoanhydride bonds A large amount of free energy is liberated when ATP is hydrolyzed to adenosine diphosphate (ADP) and orthophosphate (Pi) or when ATP is hydrolyzed to adenosine monophosphate (AMP) and pyrophosphate (PPi). The role of ATP in energy metabolism is paramount Penting ! Living organisms require a continual input of free energy for three major purposes: (1) the performance of mechanical work in muscle contraction and other cellular movements, (2) the active transport of molecules and ions, and (3) the synthesis of macromolecules and other biomolecules from simple precursors How does coupling to ATP hydrolysis make possible an otherwise unfavorable reaction? Consider a chemical reaction that is thermodynamically unfavorable without an input of free energy, a situation common to many biosynthetic reactions => In other words, coupling the hydrolysis of ATP with the conversion of A into B has changed the equilibrium ratio of B to A by a factor of about 108. We see here the thermodynamic essence of ATP's action as an energy-coupling agent. Cells maintain a high level of ATP by using oxidizable substrates or light as sources of free energy. The hydrolysis of an ATP molecule in a coupled reaction then changes the equilibrium ratio of products to reactants by a very large factor, of the order of 108. More generally, the hydrolysis of n ATP molecules changes the equilibrium ratio of a coupled reaction (or sequence of reactions) by a factor of 108n
3. The electron donor in most reductive biosyntheses is NADPH, the reduced form of nicotinamide adenine dinucleotide phosphate (NADP+; see Figure 14.13). NADPH differs from NADH in that the 2 - hydroxyl group of its adenosine moiety is esterified with phosphate. NADPH carries electrons in the same way as NADH. However, NADPH is used almost exclusively for reductive biosyntheses, whereas NADH is used primarily for the generation of ATP. The extra phosphoryl group on NADPH is a tag that enables enzymes to distinguish between high-potential electrons to be used in anabolism and those to be used in catabolism.