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INSTITUT FUR SEXUALGKONOMISCHE LEBENSFORSCHUNG KLINISCHE UND EXPERIMENTELLE BERICHTE HERAUSGEGESEN VON DR. MED. WILHELM REICH ah NETO TORE + SAE TREE OTR TS Nr. 7 WILHELM REICH 1. BION EXPERIMENTS ON THE CANCER PROBLEM 38 MICRO-PHOTOS: Il. DRE! VERSUCHE AM STATISCHEN ELEKTROSKOP CLES TP TAAL TES OTIS LE ER TIN Seapoi-Veriag, Rotterdam - sic - Kopenhagen 1939 Copyright 1939 Sexpel-Verlag, Rotterdam -Oslo-Kopenhagen ringed in Norway Sexpe. verlag, Rotcardam « Oslo = Kepenhagan BION EXPERIMENTS ON THE CANCER PROBLEM Abstracts of a lecture given to the Norweginn Society of Medical Students in Oslo, June 1938 by Wilhelm Reich, M. D. 1. THE VESICULAR DISINTEGRATION OF SOAKED AND SWOLLEN MATERIAL. J, Inorganic material, such as crystals, coke, sool, and charred blood if soaked, boiled, and autoclaved in equal parts of bouillon and KC! 0,t n, disintegrates into electrically charged vesicles. These are Bions, White hot- heating furthe mand culti ability. Tt is found that material that has hot temperature, material such as lava a vesicular structure. 1 2 Photo 1: Cryetats from common earth pussed through the autoctive at i vest mtagratinn, Bods, eultinnble an medium IV. Ga, $00 2. Photo 2: Kulinmepanide, drg, vesicular siructare, darkgronnd, en. 500 x. 3 4 Photo 3: Castiron fiting, dr Phote §, Gast iron filing after about 13 minutes in a solution of bouillon + Gin RCL aa 30. %. The iron bions showing electromagneticrtike figures tn thelr order have freed themselves, After ca, 8 days they are euitiv ble on egg medium 1 Changes in the structure of iron-filings and sand-erystals in the course of swelling in Bouillon + KCL 0,1 Sharp-edged form, on dark ground vesicular structure Development of stripes in typical forms, in striped order to be seer rhombold or quadratic. The margins be the stripes shows bluish glimmer ‘soit and curved. This heey of vesicles becomes. mobile 4 Aiting-blons of the packet-amochotd type, bluish cultivable upon ege-medium IV oF e. 2. Organie material such as tissues, musele, moss, ete. after being autoclaved in bouillon and KCI 0,1 n, disintegrates into vesicles and moving masses of vesicles. Moss growing on lava or stone shows vesicles arranged in rows (dark ground, 400—3000 x.) Infusions of dried autumn moss or grass show if observed continually with a magnification of from 400 x to 4000 x — the foliow- ing sequence of events: vesicular disintegration, after soaking and swelling separation of the vesicles in the form of cocci, concentration and organisation (via) formation of a membranous murgin) into monocellular protists of vesicular structure, For description as well as microphotos and the results of quick-motion-pietures in the micro- film vide the publication DIE BIONE, Oslo, 1938. Photo 5: Mo4s showing vesicular disintegration in water after a few days snelt- Photo 6: Protozae organising themselves of disintegrated mass. 3000 x. 5 An amoeba, drawn from nature, showing vesicular structure and direction of the stream of the plasma 4. Exactly as protists develop out of swelled disintegrated moss, the cancer-cells organize themselves in bubble-shaped disintegrated animal and human tissues, ‘The most important steps of this devel- ‘opment are according to observations until mow: Swelling of the tissue, bubble-like structuring, formation of spindel-shaped organisins, photo 8: Cancer of thenterus. The muscle tissue shows vesicular structare strongly light-bresking and biologically colourable bubble-heaps, and and different stodiama of formation of cells. 1000 finally further development into mobile, amoeboid, creeping «matures cancer-cells.. The maturing process in the mouse in experimental cancer was observed between 8 and 14 months. 304 Heatthy musele sisipat structure, no vesicles Photo 9: Cancer ofthe skin: On the left the murgin of epithetium celts in cancerous change. Ia the middte and to the right « stripe oj vesicair disintegration of epitheliaia ells. 1000 7 Photo 10: Cancer celle organising themselves ia tissue of fibuia, not get separated. Cu, 1000 st. Photo 11: Three Gancer Cells showing the typical tailed shape of -the Pipe state, bul not yet amuedoid, Ca, 8000 ‘The experimental production of Bions from sterilised or white- hot snaterial took place with two problems in view: (a) Which combination gives the best chance for the organisation and cullivation of superheated carbon? (b) What influence do Bions exert on proteins, blood, and animal tissues? mm was based on the known faet that soot and tar s (distilled, i.e. at # temperature above 600° C) stimulate cancer formation (tar-cancer in mice, chimney-sweep-eancer etc.) 8 I. BION CULTURE EXPERIMENT 6 (KOH-experiment) Mediam e: 10 ce Ringer's solution, 10 ce KCI 0,1 1 solution, and 10 drops red gelatine are dissolved in KCL 0,1 n ‘A liltle charred blood-dust on the spatula is boiled in KCI. A tyr Lezithin is dissolved in KCl-solution. The mixture of these two sub- stances is, boiled for half an hour. ‘To it is added a whole egy (sterile); the whole is then stirred and put into sterilised testtubes, adjusted to a Ph of 7.4, and left in the hot air steriliser for two hours at 90° C, The next day it is again sterilised for two hours and then left in the thermostat at 37° C for 24 hours. On this medium are poured KOH-crystals liquified in a flame. ‘The medium then becomes liquid and shows in the liquid portions cocei and bacilli, This is diluted with 10 ce equal parts of bouillon and KCI @,1 n, and put in the autoclave for half an hour. Micro organisms are visible at $000 x immediately after the preparation has passed through the autoclave, After 4 (o 8 days inoculation on egg medium 1V (q.v.) can be made. This is luid in the thermostat. Cultures are positive in about 50 % of the cases. This egy medium ¢ lends itself well to the making of cultures from solutions of Bions of soot, earth crystals, and iron filings. eS Forms, found in the EOH-preparation. —_———S Egg Medium IV. 38 Gr. potatoes thickly peeled and finely hashed. 75-¢c bouillon: 8 Gr. potato flour; 5 Gr. Lanse (White). TI boiled for half an hour, being stirred for the first five minutes until forming a pulp. {tis then cooled of to about 50° C. Then to it is added: 10 ce KEl 0.1 n, \% Gr. Lezithin, 10 ec Ringer's solution, 6 ce glycerin, 2—3 eggs (sterile, washed in alcohol). ‘The mixture is filtered through gauze and filled into sterile tubes. Ht is left in the hot air steriliser for two hours until it gets hard. ‘The next day it is left in the hot air steritiser for 2 hours at 90° C, then for 24 hours in the ther- ‘mostat for control. The Ph of these media is 7.4 NI. BION CULTURES OBTAINED FROM EXPERIMENTS 10, 12 AND 18 1. Type PA, Packet-Amoeboid, Bion Cultures from autoclaved human ‘Two to three drops of blood from a human arm vein, if shaken in 10 ce equal puris of houillon and KCI 0,1 n, and autoclaved for hi are contractile in an amoeboid fashion. Cultivation takes place on e hour at 140° C immediately produce Blood-Bions which medium IV or ¢. Positive result in about 60-70%. So tar experi Photo 12 Photo 15 10 ents have been carried out. PA-Bian-Cullure, TYPE (PA, BOPA, HAPA) amoeboid, gram +, wud. Blood Rions (10 c), blaish glimmering, obtained from antoctived jummun blood immediately ufter. preparation, amoeboid, 2000 .. HOPA und HAPA were obtained from the blood of a woian of 40 and of a man of 36, and for half treated in the auto clive, As a rale the cultures are blue aft ¢ treatment, Th microbes are of unequal size, about 2—6 ys long, and Jess than that wide, The PA-Bions grow at 37° C, they degenerate, if kept inade: into cocei like pus-staphylococe! of only 1.6—2 \. of bla id of bluish colour, They can be regenerated by rhythmical cleetriea! charges and quick inoculation on egg medium IV, They ave gram positive, obout 3 fo 4 times as big as ordinary staphylococci, ally positive, contractile, vibrating, with diffuse borderlines the individual vesicles (us seen at 4000 x); they paralyse y B, Subtilis and short gram-negative rods of the B. fluor escence group. No pathology has been observed in mice so far, Bions of the PA-type penetrate into cancer tissue, destroy cancer cells in a microscopically observable way, break up conglomerations of Br Kochii, and can be recullivated from the blood of mice, The reaction produced by them in the human organism has not yet been tested. the present time BOPA, PA and SAPA are under continuous tivation, glowed Photo 13; Another SHIT thorough inoestiquti ay 6 ” Phota 16° One single spindel-shaped cancer-cell, found in vaginal secretion tagether with one Supa Bion Photo 17; Two sphere-shaped cancer-cells huping been killed by Sapa-Bions on distance. Before the picture was taken, (he cancer-cells crept arcunt ia an amoeboid way and had been filmed. They originated in the glands of a mouse. Photo 18; Heaps of haman cuncer-celts penetrated ani partially destroyed by Ropw-Bions under the microscope. The cancer-elle show eolont of Methglen-blue 2. Type BLURO (Blut and Kohie): small cells with nuclei and proto: plasmatic structure, derived from Preparation 13, Charred blood preparation (10¢) is added to the autoclaved blood- bion preparation. About 2 gr. each of soot or charred blood, which, inely powdered, has been in the hot sic steriliser for 4 hours at 180° Cy are heated white hot and immediately put into 24 hours old blood- 12 bion preparation and shaken. Or the charred bloud is put into 10 ec of equal parts bouillon and KCI 0,1 n and added liter. Purther activ= ation of the BLUKO preparation takes place through the addition of 1 co blood serum or sterile egg white. The prepation is put in the thermostat for 24 hours, and then kept at room emperature for 6 to 8 days. Daily microscopic observation (at 3000 x) reveals a mutual pene= tration of carbon and blood Bions, the fornution of vesicular micro- bes, round cells with nuclei (at 34000 x), and bluish glimmer (Apo- chromat-Objective) (Vide Photos), Inoculation on egg medium 1V is strong from the 8th day on. The media are then laid in the ther- mostat at 37°C. Thus far there have been 85 experiments with 80 % positive results. As a rule blood agar, afler passage over the egg medium showed a blue-grey growth; microscopically this proved to consist of contractile heaps of large vesicles, which in some cases flowed together and showed cell formation. Strains I, V, VIL X. XIII, and XIV of the BLUKO-Bion-Cultures showed — after weeks in unopened test tubes BLUKO-cells. These grow quickly at room temperature by way of division, and they show a creamy appearance with gray-white, salmon-red or yellow-brown colour. They react to Gram and Eosin-Haematoxylin stain. Piva 19: SEROT Bion Culture, leiny, oblained from prepurat 12/4, ea ‘000. Photo 20: Seko-Blon-Celle gram coloured, ca. 600 2, Vide the different shape amd sfructure when deal? 13 ma m of simi preparation, of infection as in the yrowth ur microstructures at the «1 so far in some cases in infiltrating eelluls in kidne:’s, glands, liver ete LUKO Bion celts, culture, obtained from prep. 13, grameotoured, BLUKO 8 from prep. 13, 9 ies 00, One single Minko-cell, Hoing, ea. 4300 x Photo 24 Phato 2 Photo 26: Bions from eharred cout (10 €) immediatly after preparation. Byy-medinm is being penetrated by char-eoul bions, (Caneer-matel experiment 1H) Photo 23, Photo 2 coneluded: so far, sub- months in. cell-mnas ¢ deposits in peritoneum, liver, nents are not yet The animal cutaneous injection resulted after 4~ subeutancous tissue with metastasis-lik experi re being injectio 14 Photo 97 fy some experiments with mice subeutaneots of Bions from whitesheated coal alone resulted in the f ontinued, Boncells in organisation on egg medium IV. Model experiment regard ing the woot cancer Bion celts have caused a tumor on d mause Cancer-model-experiment in the glands of the throat. ton the teft aide of the throu originating Heups of Bion cette in the omentum of a mouse injected with Sekort: elie, (Vide pletuce 19). IV. THE T-BACILEL These organisms resull from degeneration of certain types of Bion- Cultures, and from experimental degeneration of e proteins ‘hey are culled T, ie. «Tod>-/Dedth-) Bacilli because of their origin and thelr deadly effect on mice, They are short flitting rods of about 0.25 0.6. Seen under u anugnifieation of about 4000 x they show slightly oval shape, or else two of thent form an elongated double club. On light agar they grow turflike quickly at 37° C, show a blue «lim mer aginst light, and after some weeks they colour houilion bluish ‘They have a shurp acid and ammoniu-like smell. In big doses they kill mice within 24 hours, In light doses they produce in the course of from 8 to 15 months infiltrating and destructive growths in the form of tumours, or only cellular growths without circumscribed primary tumour in kidneys, lungs, liver, glands ete. ‘They can he recultivated in pure culture from the cardiae blood of mice which died or were killed, und if again inoculated, they produce the same pathologi changes as before, Photo 28: T= Hach (10eT) Bloost-Coal immediatiey after having been glores aid put {n solution of outtlon KCL hows, when gramestoured, very xmall aloagated boeiltt in ritdiah colour. Refore the pointer three T-bncilit, Apparant size st about 5000 a. Real size 0, we The photo ie taken mith 150 x Aprochrom. Object, & x Ocul, 30 % eniarging binacular tube: 1800 x. Three timen enlarged on the sop: F400 a. The blywce particles are small eval binns of Blutsh glinamer, find coal paiticies not developed nto biens, Vholo > T