CALCULATION OF GENE AND GENOTYPE FREQUENCY OF MULTIPLE

ALLELE

In a population of I M.Sc., Zoology the following blood group were observed among the
students and was used for the calculation of gene frequencies and genotype frequencies by
applying Hardy-Weinberg law.

Total number of Strength

= 20

Number of A Group

= 01

Number of B Group

= 08

Number of AB Group

= 02

Number of O Group

= 09

Percentage Calculation:

A group

-5%

- 0.05

B group

- 40%

- 0.40

AB group

- 10%

- 0.10

0 Group

-45%

- 0.45

Calculation of Gene frequency :

Let us assume, A = p;

B= q; O= r

Percentage of O group (ii) r2 = 0.45

Frequency of recessive allele,
O = r2 = 0.45
r=

0.45

= 0.670

A group persons (I AIA + IAIi)

P2 + 2pr = 0.05
= p2 +2pr + r2 + 0.05 +r2
(p+r)2 = 0.05 +0.45
p+r=
=

0.05+0.45

0.50

= 0.707
P = 0.707 0.67
= 0.037

To find out the frequency of q, we subtract the sum of p and r from 1 since p + q + r = 1
q = 1 (p + r)
= 1 0.670 +0.037
q = 1 - 0.707
= 0.293

RESULTS:
Gene Frequency of
A Group 0.037
B Group 0.293
0 Group 0.670
1.000

Genotype Frequency of
LALA = p2 =
LALO = 2pr =
LB LB = q 2

LBLO = 2qr =
LALB = 2pq =
LOLO = r2 =
Genotype Frequency of
A Group =
B Group

AB Group =
O Group =