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African

Hemorrhagic
Fever virus

By: Justine Ann Jacbang

Marburg

Ebola

Group

Group V (-)
ssRNA

Group V (-)
ssRNA

Order

Mononegavirales

Mononegavirales

Family

Filoviridae

Filoviridae

Genus

Marburgvirus

Ebolavirus

Classification

800 14000 nm in length with a uniform


diameter of 80 nm
Enveloped
Non-segmented
Negative-stranded RNA viruses
Helical
Highest mortality rates (25 90%)

Morphology

Ebola virus

Marburg virus

Cytoplasm
The virus attaches to
the receptors of a host
cell using a glycoprotein
called peplomer. The
virus is then engulfed
by endocytosis.

Replication

The virion envelope and


cellular membrane of host
fuses together and the virus
nucleocapsid is released.

Virus uncoats and the


nucleocapsid is transcribed
onto positive stranded
mRNA which are translated
into viral proteins.

Glycosylation occurs in
which viral proteins attach to
glycans and the glycoprotein
precursors are cleaved into
glycoprotein 1 and 2.

Glycoproteins 1 and 2
assembles together to form
heterodimers. In the
meantime, a negative stranded
RNA strand is synthesized
to be used as a template to
createa positive single
stranded RNA.

Heterodimers multiply to
form trimers that will form
the surface of peplomers of the
emerging viral cells.

New viral copies are made


from the negative stranded
RNA strand and the trimers
make their way to the cell
membrane.

Formation of nucleocapsids
and viral envelope occur
when trimers (viral proteins)
and new genomes gather
around the edges of host cell
in preparation for budding.

Newly formed viruses bud


from the host cell, gaining the
cells membrane. Mature
progeny are free to infect
other cells.

A.

Disease produced

1. Marburg hemorrhagic fever (MHF)


2. Ebola hemorrhagic fever (EHF)
Signs and symptoms
. fever, chills, headache, andmyalgia(muscle aches)
. Nausea, vomitting, and chest or abdominal pain,
maculopapularrash(with both of flat and raised
lesions)
. External and internal bleeding, often leading to shock
and death
.

Incubation period for MHF: 3 9 days


.Incubation period for EHF: 2 21 days
.

Pathogenesis

B. Modes of Transmission

Direct contact with an infected animal


Direct contact with the blood and/or secretions
of an infected person
Nosocomial transmission

Enzyme-linked immunosorbent assay (ELISA);


Immunofluorescent Technique
Polymerase chain reaction (PCR) assay
Virus isolation

Laboratory Diagnosis