Neuronal network topology governs

the emergence of stereotyped circuit dynamics

75 . 0 2

LL31

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2

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P. B . K r u s k a l ,
S. J. Weinberg,
A . J . S a d o v s k y,
J.N. MacLean
1 C o m p u t a t i o n a l N e u r o s c i e n c e , 2 P h y s i c s , 3 D e p a r t m e n t o f N e u r o b i o l o g y, U n i v e r s i t y o f C h i c a g o

Introduction

Spiking Model

80 Excitatory and 20 inhibitory Izhikevich model neurons

These dynamics likely reflect the underlying synaptic connectivity, or
topology, within functional circuits.

Excitatory topology in the postsynaptic pool is varied

Feed forward random activity from a presynaptic pool multiple times

It remains unclear which specific topological classes are capable of
supporting the observed spatiotemporal activity.
2+
HOPS Multiphoton Calcium Imaging
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-2

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ms

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8 10

0.02
0.5
0.3
0.4
0.6
Average distance metric

σ1

temporal
variances

σ2

1,

σ 2,...σ n

}

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50

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0.2
0.5
clustering coefficient

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Frame 3
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SF

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0.1
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0.7

0.1

0.2
0.5
clustering coefficient

0.7

0.1

ER

SW

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0.2
0.5
clustering coefficient

0.7

SF
70

60

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40
Time in ms

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18

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a

Conclusion

Iterative application of the Hartigan Dip Test allows for high
throughput screening for spatiotemporal stereotypy
in circuit reactivations.

Stereotypy is inversly related to in degree at the level
of individual neurons

1500
100 0

16

m
Sig

Scale free topologies exhibit more precise sequences
than small world and Erdős–Rényi topologies.

20

80

12
8
sigma in ms

14

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60

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60
40
Time in ms

4

20

12

6

2

All circuit topologies support stereotyped sequences.

40

20

0

30

8

4

Calcium imaging in slices of mouse sensory cortex reveals
stereotyped and recurring sequences of neuronal activation
within local neocortical circuits.

SF

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0

12
8
sigma in ms

60

SF

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60

4

100

150

Spiking Dynamics

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Frame 4

0 2 4
Seconds

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0.1

1200

-4 -2

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Count

Count
Frame 2

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In degree

ER

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600

Frame 3

-4 -2

20

inhibitory

Trial number

Experimental spike trains
Prob. of spike

Shuffled spike trains

Clustering with Hardigens
Dip Test

10

0.001

0

ee

8 10

80

gr

6

40
60
In degree

de

4

0.003

Probability

300

Time in ms

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20

SW

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-4 -2

40
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In degree

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In

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1

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Distributions of clustering coefficients per neuron for five ‘constructions’

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Temporal Analysis

Pairwise Victors metric
bootstrap reshuffling

0

Count

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Neuron #

2
4
Seconds

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10

inhibitory

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Count

-2

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Neuron #

200
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inhibitory

-2

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Neuron #

MUA

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Frame 1

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16

ER

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12
8
sigma in ms

Probability

200

SF

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4

140

Count

Observed sequence

ER

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Count

300

0.04

SW

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Count

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Frame 4

Distributions of in degrees per neuron for five ‘constructions’

Count

Neuron #

500

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1

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600

0.005

SW

Time in ms

Count

pi

mV

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Temporal variance from
Hadigens Dip Test

Imaging

circuit activity

10

Frame 2

HOPS Ca

Scale Free (SF)

Erdős–Rényi (ER)

Small World (SW)

Time Dependent
Functional Topology (ER)

Structural Topology (ER)

Frame 1

Repeating sequences of neuronal activity are observed across multiple
brain states, preparations, and systems.

Simulation Analysis

100 0

20

60
40
Time in ms

80

100

This work was supported by the DANA Foundation (JNM)
and National Science Foundation CAREEER Award
0952686(PBK, JNM), National Institute of General
Medical Sciences Grant GM007839 (AJ S).