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Biotechnology

Kevin Elkin
Josh Chapman

The Task
Create a device that would help law
enforcement in forensics such as scientific
tests that help to investigate potential
victims of a crime.
The device should encompass 21st century
skills and technology to ensure the device is
both effective and modern. In addition, the
device should enhance both the mobility
and efficiency of current lab equipment.

Approach
Plan

Communicat
e

Evaluate

Design

Create

Final Solution and


Explanation

Utilize an Arduino board to effectively hook up


the pumps, motor, and LED indicators.
3D printer to print out the canister to hold the
various chemicals and biological materials.

Specifications of Final
Design
Dimensions: 6 inches tall, 5 inches deep, 13
inches long
Motor: 9300 RPM
Pumps: 1.1 mamps

How it Works
Extraction Phase

Nanoball Phase

Purpose: To extract the DNA from the


chosen cell specimens

Purpose: To analyze the DNA by their


nucleic acids.

Add lysis solution to begin dissolving the


phospholipid bilayer of the cell membrane.
Once the cell has been destroyed, collect
all other remaining cell parts with a salt
solution.
Spin remaining contents in centrifuge to
separate the heavier biological material
from the lighter material.
Add a replicator solution containing tagged
nucleotide bases, DNA polymerase and
helicase to create analyzable DNA strands.
(Note: The heating during this phase
speeds up the process)

Pour the finished solution into the Nanoball


micro array tray, the microarray will
analyze the DNA and save it for further use.
fluorescent probes (which are fluorescent
chemical compounds able to re-emit light
upon light extraction) bind to the base
pairs of the DNA.

Advantages to the Solution


Our solution/device would encompass advantages
such as the following:

Compact and portability ensures storage in virtually


any law enforcement vehicle

Precise and accurate measurements allow for an


effective mobile laboratory

Cost effective and time consuming benefit law


enforcement personnel both economically and in
time sensitive cases

Real Life Applications


Potential real life applications to our design
encompass the following:

Beneficial when finding diseases

Able to discover genetic mutations

Helpful in tracing DNA of suspects in


criminal cases

Summary
The DNA toolbox incorporates everything a
typical lab set-up would require in order to
extract and analyze DNA
Our device will be ultimately a cost-effective and
rapid solution that allows law enforcement
personnel to analyze DNA
Our device is designed to encompass a user
friendly interface to permit the user to analyze
DNA with ease
The overall build of the device is both accurate
and precise, as if it was a high quality laboratory

Works Cited (MLA)

"Advanced Genetic Technology - DNA Analysis, Inc." Advanced Genetic Technology - DNA
Analysis, Inc. N.p., n.d. Web. 19 Feb. 2015.

"BIO | Healing, Fueling and Feeding the World." BIO | Healing, Fueling and Feeding the World.
N.p., n.d. Web. 19 Feb. 2015.

"DNA Evidence: Basics of Analyzing." National Institute of Justice. N.p., n.d. Web. 11 Feb. 2015.

"DNA Extraction." DNA Extraction. N.p., n.d. Web. 13 Feb. 2015.

Harris, William. HowStuffWorks. HowStuffWorks.com, n.d. Web. 19 Feb. 2015.

"MinION: A Complete DNA Sequencer on a USB Stick." Quazoo. N.p., n.d. Web. 12 Dec. 2015.

"MinION: A Complete DNA Sequencer on a USB Stick." Quazoo. N.p., n.d. Web. 19 Feb. 2015.

PBS. PBS, n.d. Web. 19 Feb. 2015.

"What Is DNA?" Genetics Home Reference. N.p., n.d. Web. 11 Feb. 2015.