B NNG NGHIP

V PHT TRIN NNG THN

CC QUN L CHT LNG
NNG LM SN V THU SN
S:

534

/Q-QLCL

CNG HO X HI CH NGHA VIT NAM

c lp- T do- Hnh phc

H Ni, ngy 31

thng 12

nm 2013

QUYT NH
Ch nh i vi Phng th nghim cht lng nng lm thu sn thc phm
CC TRNG
CC QUN L CHT LNG NNG LM SN V THU SN
Cn c Quyt nh s 29/2008/Q-BNN ngy 28/1/2008 ca B trng B
Nng nghip v Pht trin nng thn quy nh chc nng, nhim v, quyn hn v c
cu t chc ca Cc Qun l Cht lng Nng lm sn v Thu sn;
Cn c Thng t s 16/2011/TT-BNNPTNT ngy 01/4/2011 ca B trng B
Nng nghip v Pht trin Nng thn quy nh v nh gi, ch nh phng th
nghim ngnh nng nghip;
Xt Bin bn nh gi li v m rng phng kim nghim cht lng nng lm
thy sn vng 4 ngy 17/9/2013 ca phng th nghim v Bo co thc hin khc
phc ngy 25/12/2013ca Trung tm Cht lng nng lm thy sn vng 4;
Xt ngh ca Trng phng Qun l Kim nghim,
QUYT NH:
iu 1. Ch nh phng th nghim ca Trung tm Cht lng nng lm thy
sn vng 4 (a ch: 30 Hm Nghi, Qun 1, Tp. H Ch Minh in thoi: 08 3821
0815, Fax: 08 3914 2161) l phng th nghim cht lng nng lm thy sn thc
phm.
iu 2. Phng th nghim nu ti iu 1 c cp m s l: LAS-NN 87. Quy
cch v vic s dng con du v m s ca Phng th nghim c quy nh ti Ph
lc 5 Ban hnh km theo Thng t s 16/2011/TT-BNMPTNT ngy 01/4/2011 ca B
Nng nghip v Pht trin Nng thn.
iu 3. Phng th nghim nu ti iu 1 c tham gia phn tch cc ch tiu
cht lng nng lm thy sn trong phm vi nu ti Ph lc 1 theo yu cu ca cc t
chc chng nhn cht lng sn phm nng lm thy sn thc phm, cc c quan
qun l nh nc v cht lng nng lm thy sn v ca cc t chc, c nhn sn
xut, kinh doanh sn phm nng lm thy sn thc phm.
iu 4. Quyt nh ny c hiu lc 03 (ba) nm k t ngy k.

iu 5. Cc ng Chnh Vn phng, Trng phng Qun l Kim nghim, Th

trng cc c quan, n v c lin quan v Gim c Trung tm Cht lng nng lm
thy sn vng 4 chu trch nhim thi hnh Quyt nh ny.
Ni nhn:
- Nh iu 5;
- Cc trng (bo co);
- V KHCN (bo co);
- TT T.tin ( ng ti website);
- Lu VT, KN.

KT. CC TRNG
PH CC TRNG
( c)
L B Anh

Ph lc 1: Danh mc cc ch tiu/ nhm ch tiu ch nh ca

Phng th nghim thuc Trung tm Cht lng nng lam thy sn vng 4
(ban hnh km theo Quyt nh s
/Q-QLCL ngy thng nm 2013
ca Cc Qun l Cht lng Nng lm sn v Thy sn)
I. Lnh vc cc php th: Ha hc
Tn php th

i tng
php th

Phng php th

Gii hn pht
hin (nu c)/
Phm vi o

1.

Xc nh hm lng m v Tro

Thc phm

NMKL 23, 3rd, 1991

v NMKL 173, 2005

2.

Xc nh hm lng Nit Bay

Hi (TVB-N)

Thy sn,
sn
phm thy
sn

Chng III quyt

nh EC 2074/2005
ngy 05/12/2005

Thc phm

NMKL 89, 1986

Thc phm

NMKL 132, 1989

10mg/kg

Thc phm

NMKL 57, 1994

TT

3.
4.
5.

Xc nh hm lng mui
Clorua
Xc nh hm lng Sulfit
Xc nh hm lng Phospho
tng/P2O5

6.

nh tnh Poly-phosphat tng

Thc phm

05.2 CL4/ST3.10
(Ref. ISO5553:1990)

500 mg/kg

7.

Xc nh hm lng Protein
tng

Thc phm

NMKL 6, 2003

Thy sn,
sn phm
thy sn

TCVN 3706-1990

Thc phm

NMKL No.131.1989
(NAF 047/11)

Thc phm

AOAC 959.09

0.02%

Thc phm

NAF 019/10

0.2%

NAF 018/10

0.1%

8.

Xc nh hm lng Amoniac

9.

Xc nh hm lng m

10.
11.

nh tnh Acid boric, mui

borat
nh tnh tp cht Agar

12.

Pht hin nhanh tp cht Agar

13.

Pht hin nhanh tp cht tinh

bt v PVA

14.

Pht hin nhanh tp cht CMC

15.

Xc nh hm lng nit axit

amin

16.

Xc nh hm lng axit

17.

o pH
Xc nh hm lng Acid
Benzoic, Sorbic bng HPLCPDA/UV-VIS

18.

Tm nguyn
liu
Tm nguyn
liu
Tm nguyn
liu
Thy sn v
cc sn
phm thy
sn
Thy sn v
cc sn
phm thy
sn
Thc phm
Thc phm

NAF 020/10

0.03%

NAF 040/10

0.2%

TCVN 3708:1990
(NAF 026/10)

TCVN 3702:2009

AOAC 981.12
05.2 CL4/ST3.54
(Ref. NMKL
No.124, 2ed, 1997)

5 mg/kg

TT

Tn php th

i tng
php th

Phng php th

Gii hn pht
hin (nu c)/
Phm vi o

Thy sn,
sn phm
thy sn

05.2 - CL4/ST3.97
(Ref. TCVN
8025:2009 &
ScienDirect Journal
of Chromatography
A 1161 (2007) 207213)

10 mg/kg

NAF 050/12
(Ref. AOAC 977.13)

1 mg/kg

19.

Xc nh hm lng Urea
bng HPLC-FLD

20.

Xc nh hm lng Histamine
bng HPLC-FLD

21.

Pht hin Histamin bng Elisa

22.

Xc nh hm lng ASP bng

HPLC-PDA/UV-VIS

23.

Xc nh hm lng
Tetracycline group (TC, OTC,
CTC, DC) bng HPLC-FLD &
HPLC-PDA

Thc phm
05.2 - CL4/ST3.55
c ngun
(Ref. AOAC 995.09)
gc ng vt

10.0 g/kg
(OTC, TC)
20.0 g/kg
(CTC, DC)

Xc nh hm lng nhm
Tetracyclins (TC, OTC, CTC,
DC) bng LC-MS/MS

05.2 - CL4/ST3.90
(Ref. Method from
service de protection
de la consommationThc phm
Geneve, chapitre 9c ngun
Unit: pesticides et
gc ng vt
mycotoxines.
J.Chromatogr
(623:1992) paper
153-158)

20 g/kg cho
mi cht

Thc phm
c ngun
gc ng vt
& thc vt

NAF053/13
(Ref. AOAC
2007.01)

2.0 g/kg cho

tng cht

05.2 - CL4/ST3.62
(Ref. ScienceDirect Journal of
Chromatography A
898 (2000) 95-102)

20 g/kg cho
tng cht

24.

25.

26.

Xc nh hm lng thuc bo
v thc vt gc Chlor (HCB,
Aldrin, alpha-BHC, beta-BHC,
delta-BHC, gamma-BHC,
alpha-Chlordane, gammaChlordane, 4.4-DDE, 4,4DDT, Dieldrin, Endosulfan I,
Endosulfan II, Endosulfan
sulfate, Endrin, Endrin
aldehyde, Endrin ketone,
Heptachlor, Heptachlor
epoxide) bng GC-ECD
Xc nh hm lng
Sulfonamides
(Sulfachloropyridazine (SCP),
Sulfamethoxazole (SMTX),
Sulfathiazole (STZ),
Sulfamerazine (SMR),

Thy sn,
sn phm
thy sn
Thy sn,
sn phm
thy sn
Thy sn,
sn phm
thy sn

Thc phm
c ngun
gc ng vt
Mt ong

05.2 - CL4/ST3.88
(Ref. Method of
LDN elisa test kit)
NAF 033/10
(Ref. IOC Manuals
and Guide No.33,
1995)

50 mg/kg

0.2 mg/kg

TT

27.

Tn php th
Sufadiazine (SDZ),
Sulfamethazine/Sulfadimidine
(SMT), Sulfadimethoxine
(SDT)) & Trimethoprime bng
HPLC-PDA
Xc nh hm lng
Sulfonamides
(Sulfachloropyridazine (SCP),
Sulfamethoxazole (SMTX),
Sulfathiazole (STZ),
Sulfamerazine (SMR),
Sufadiazine (SDZ),
Sulfamethazine/Sulfadimidine
(SMT), Sulfadimethoxine
(SDT)) & Trimethoprime bng
LC-MS/MS

28.

Xc nh hm lng
Chloramphenicol bng LCMS/MS

29.

Pht hin Chloramphenicol

bng Elisa

30.

i tng
php th

Phng php th

Gii hn pht
hin (nu c)/
Phm vi o

Thc phm
c ngun
gc ng vt
Mt ong

05.2 - CL4/ST3.89
(Ref. ScienceDirect Journal of
Chromatography A
898 (2000) 95-102)

20 g/kg cho
tng cht

Thc phm
c ngun
gc ng vt
Mt ong

Thc phm
c ngun
gc ng vt
Thc phm
Xc nh hm lng Flofenicol
c ngun
bng LC-MS/MS
gc ng vt

31.

Xc nh hm lng
Nitrofurans (AOZ, AMOZ,
SEM, AHD) bng LC-MS/MS

Thc phm
c ngun
gc ng vt

32.

Pht hin cc cht chuyn ha

Nitrofurans (AOZ, AMOZ)
bng Elisa

Thc phm
c ngun
gc ng vt

33.

34.
35.

Xc nh hm lng Green
malachite, Leuco malachite
green, Crystal violet &
leucocrystal violet bng LCMS/MS
Pht hin malachite green
(Total): Malachite green &
Leuco malachite green bng
Elisa
nh tnh Lipophilic (okadaic
acid,
dinophysistoxins,
pectentoxins, azaspiracids v
yessotoxin) bng sinh ha trn

Thy sn v
sn phm
thy sn
Thy sn,
sn phm
thy sn
Thy sn,
sn phm
thy sn

05.2 - CL4/ST3.67
(Ref.
DFS/ORA/FDA LIB
No.4306; Analytica
Chimica Acta 483
(2003),
125 135)
05.2 - CL4/ST3.93
(Ref. Method of
TABP elisa test kit)
05.2 - CL4/ST3.84
(Ref. USDA, CLGFLOR1.02)
05.2 - CL4/ST3.68
(Ref. US
FDA/CFSAN 19062006)
05.2 - CL4/ST3.94
(Ref. Method of Bioo
Scientific elisa
testkit)
05.2 - CL4/ST3.71
(Ref. J.AOAC
Vol.88, No.5, 2005)
05.2 - CL4/ST3.87
(Ref. Method of Bioo
Scientific elisa
testkit)
NAF 035/11
(Ref. IOC Manuals
and Guide No.33,
1995)

0.1 g/kg

0.2 g/kg

0.1 g/kg

0.5 g/kg

0.5 g/kg

0.5 g/kg

1g/kg
-

i tng
php th

Phng php th

Gii hn pht
hin (nu c)/
Phm vi o

Xc nh hm lng PSP bng

sinh ha trn chut

Thy sn,
sn phm
thy sn

NAF 034/10
(Ref. AOAC 959.08)

Xc
nh
hm
lng
Trichlorfon & Dichlorvos bng
LC-MS/MS

Thy sn,
sn phm
thy sn

05.2 - CL4/ST3.78
(Ref. Method of
Bayer AG)

Trichlorfon: 5.0
g/kg
Dichlorvos:
15.0 g/kg

TT

Tn php th
chut

36.

37.

38.

Xc
nh
hm
lng
Thc phm
Diethylstibestrol (Des) &
c ngun
Methyltestoterone
(Methyl)
gc ng vt
bng LC-MS/MS

39.

Xc nh hm lng Macrolide Thc phm

group (Spiramycin, Tylosin)
c ngun
bng LC-MS/MS
gc ng vt

40.

41.

42.

43.

44.

45.

05.2 - CL4/ST3.79
(Ref. Method of RBiopharm
&Analytica Chimica
Acta 483 (2003),
269 280)
05.2 - CL4/ST3.82
(Ref. Method of
Wakopak Wakosil &
Analytical Chimica
Acta 473 (2002)
167-175)
05.2 - CL4/ST3.63
(Ref. US FDA
DFS/ORA No.4039)

Xc nh hm lng Oxolinic
Thc phm
acid v Flumequine bng
c ngun
HPLC-FLD
gc ng vt
Pht hin Fluoroquinolones
tng: Enrofloxacin;
Thc phm
05.2 - CL4/ST3.86
Ciprofloxacin; Sarafloxacin;
c ngun
(Ref. Method of
Dannofloxacin; Difloxacin;
gc ng vt Abraxis elisa testkit)
Norfloxacin bng Elisa
Xc
nh
hm
lng
Quinolones
&
05.2 - CL4/ST3.83
Fluoroquinolones
Thc phm
(Ref. Jounal of Food
(Enrofloxacin, Ciprofloxacin,
c ngun
and Drug Analysis
Sarafloxacin,
Norfloxacin, gc ng vt
Vol.18, No.2, 2010,
Flumequine, Oxolinic Acid,
Mt ong
Pages 87-97)
Dannofloxacin,
Difloxacin,
Pefloxacin) bng LC-MS/MS
Thc phm
Xc nh hm lng Aflatoxin
05.2 - CL4/ST3.85
c ngun
(B1, B2, G1, G2) bng HPLC(Ref.: AOAC 993.17;
gc ng vt
FLD
AOAC 972.26)
v thc vt
Thc phm
Xc nh hm lng Aflatoxin
05.2 - CL4/ST3.91
c ngun
(B1, B2, G1, G2) bng LC(Ref.: AOAC 993.17;
gc ng vt
MS/MS
AOAC 972.26)
v thc vt
05.2 - CL4/ST3.98
Thc phm
Xc nh hm lng Melamine
(Ref. FDA LIB
c ngun
bng LC-MS/MS
No4421-4422
gc ng vt
10/2008)

0.5 g/kg
(DES)
0.2 g/kg
(Methyl)

40 g/kg

5g/kg

0.5 g/kg

0.5 g/kg
(Thc phm c
ngun gc
ng vt)
2 g/kg (Mt
ong)
1g/kg cho
tng cht
1.0 g/kg cho
tng cht

50 g/kg

TT

Tn php th

i tng
php th

46.

Xc nh hm lng Trifluralin
bng GC-MS

Thc phm
c ngun
gc ng vt

47.

Xc
nh
hm
lng
Praziquantel bng LC-MS/MS

Thc phm
c ngun
gc ng vt

48.

Xc nh hot phng x tia Thc phm

gamma (Cs-134; Cs-137; Ic ngun
131, K-40, U-238, Th-232.) gc ng vt
bng ph k gama HPGe
& thc vt

49.

50.

51.

Xc
nh
hm
lng
Thc phm
Clenbuterol, salbutamol &
c ngun
Cractopamine
bng
LCgc ng vt
MS/MS
Xc nh hm lng kim loi:
Thc phm
Pb, Cd, Hg, As, Cr, Mn, Fe,
c ngun
Co, Ni, Cu, Zn, Se, Sr, Ba, Sn,
gc ng vt
K, Na, Al, Mg, Tl, Te, Li, Bi,
v thc vt
Be, B, Ga bng ICP-MS
Thc phm
Xc
nh
hm
lng
c ngun
Ethoxyquin bng LC-MS/MS
gc ng vt
Xc nh hm lng thuc bo
v thc vt bng LC-MS/MS
Abamectin

Phng php th
05.2 CL4/ST3.101
(Ref. AOAC
2007.01)
05.2
(CL4/ST3.102
Ref. Journal of
Bioanalysis &
Biomedicine, Vol.2,
Issue 5, 096-099
(2010))
05.2 - CL4/ST3.105
(Ref. AOAC 996.05
& Genie 2000)

05.2 - CL4/ST3.103
(Ref. Method of
Agilent manufactore
5990-8788EN)

05.2 CL4/ST3.106
(Ref. AOAC 999.10)
05.2 CL4/ST3.112
(Ref. Analytical
Chemistry 68, 1996)

Carbendazim/Benomyl

52.

62.

Carboxin
Cymoxanyl
Cyromazine
Dinotefuran
Imidaclopid
Indoxacard
Metalaxyl
Thiamethoxam
Xc nh hm lng thuc bo
v thc vt (Chlorothanil;

Thc phm
c ngun
gc thc vt,
Tht, thy
sn, Mt ong

05.2 - CL4/ST3.99
(Ref. AOAC
2007.01)

Thc phm
c ngun

05.2
(CL4/ST3.100

Gii hn pht
hin (nu c)/
Phm vi o
0.8g/kg

5.0 g/kg

3 Bq/kg (U-138
& Th-232)
1Bq/kg cho
tng ng v
0.2g/kg (thc
phm c ngun
gc ng vt)
cho tng cht.
0.05g/kg
(sa) clen
5.0 g/kg cho
tng ch tiu

5.0 g/kg

10 g/kg
10 g/kg (Thc
phm c ngun
gc ng vt);
2 g/kg (Mt
ong)
6 g/kg
20 g/kg
10 g/kg
4 g/kg
5 g/kg
30 g/kg
1 g/kg
10 g/kg
2.0 g/kg

TT

Tn php th
Cypermethrin; Fipronil; 4,4DDT) bng GC-MS

63.

64.

Xc nh hm lng Multiresidue Pesticides bng GCMS/MS v LC-MS/MS

Xc nh hm lng Nitrat &
Nitrite bng HPLC-PDA

i tng
php th

Phng php th

gc thc vt

Ref. AOAC 2007.01)

Thc phm
c ngun
gc thc vt
Thc phm
c ngun
gc thc vt
& ng vt

NAF 052/13
(Tiu chun Nht
bn)
TCVN 7814:2007
(EN 12014-2:1997)

Gii hn pht
hin (nu c)/
Phm vi o

3.0 g/kg
10 mg/kg
(Nitrate)
15 mg/kg
(Nitrite)

II. Lnh vc cc php th: Sinh hc

TT

Tn php th

1.

Tng s vi sinh vt hiu kh

(TPC)

2.

Tng s Coliforms

4.

6.
7.

nh lng E. coli

9.

nh tnh E. coli

10.

nh lng Staphylococcus
aureus/Staphylococci
coagulase dng tnh

12.

nh tnh Staphylococcus
aureus/ Staphylococci
coagulase dng tnh

13.

nh tnh Salmonella spp.

14.

nh tnh Shigella

15.

nh tnh Vibrio cholerae

16.
17.

nh tnh Vibrio
parahaemolyticus
nh lng Vibrio
parahaemolyticus

Phng php th
NMKL 86:2006
ISO 4833: 2003
NMKL 44:2001
ISO 4832: 2006
ISO 4831:2006

nh tnh Coliforms
nh lng Coliform chu
nhit, F.coliforms,E. coli
nh lng Fecal coliform

5.

i tng
php th

ISO 4831:2006

Thc
phm,
nguyn
liu ch
bin thc
phm

CFU/g
CFU/g
MPN/g
nh tnh/g
(hoc 10g)

NMKL 96: 2003

MPN/g

NMKL 125:2005
ISO 16649-2:2001
ISO 7251:2005
ISO 16649-3:2005

CFU/g
CFU/g
MPN/g hoc
MPN/100g
nh tnh/g
(hoc 10g)

ISO 7251:2005
NMKL 66: 2003
ISO 6888-1:1999/
Amd1:2003
ISO 6888-3:2003
ISO 6888-3:2003

Thc
phm,
nguyn
liu ch
bin thc
phm

Gii hn pht
hin (nu c)/
Phm vi o

NMKL 77:1999
ISO 6579:2002/
Amd 1:2007
NMKL 151:1995
ISO 21567:2004
FDA - 2004
ISO 21872-1: 2007
FDA - 2004
ISO 21872-1: 2007
04.2CL/ST 3.24
04.2CL/ST 3.24

CFU/g
MPN/g
nh tnh/g
(hoc 10g)
nh tnh/
25g
nh tnh/ 25g
nh tnh/ 25g
nh tnh/
25g
CFU/g v
MPN/g

18.
19.
20.
21.

Listeria monocytogenes
nh
lng
Listeria
monocytogenes
nh lng nm men, nm
mc
nh
lng
Enterobacteriaceae

ISO 11290-1: 1996/

Amd 1:2004
ISO 11290-2: 1998/
Amd 1:2004
NMKL 98:1995
ISO 21527-2:2008
NMKL144:1992/
Amd 2005
ISO 21528-2: 2004

nh tnh/
25g

ISO 7937:2004

CFU/g

ISO 7932:2004

CFU/g
nh tnh/
25g

23.

nh
lng
Clostridium
perfringens
nh lng Bacillus cereus

24.

nh tnh Campylobacter

ISO10272-1:2006

25.

nh lng vi khun kh
sulfite

NMKL 56: 1994

ISO 15213:2003

26.

nh tnh vi khun kh sulfite

04.2CL/ST 3.39

27.

nh tnh E.coli O157

Tng s vi khun hiu kh
220C v 370C
Tng s Coliforms,
F.coliforms, E.coli
Tng s lin cu khun phn
(Enterococci)

ISO 16654:2001

22.

28.
29.
30.
31.

nh lng Clostridium
perfringens

32.

nh lng Pseudomonas
aeruginosa
Pht hin Vibrio cholerae O1

33.
34.

Pht hin E.coli O157 bng k

thut PCR

ISO 6222: 1999

CFU/g
CFU/g
CFU/g

CFU/g
nh tnh/g
(hoc 10g)
nh tnh/
25g
CFU/ml

ISO 9308-1:2000

CFU/100ml

ISO 7899-2:2000

CFU/100ml

04.2CL/ST3.19

CFU/100ml

ISO 16266: 2006

CFU/100ml

04.2CL/ST 3.49

nh tnh/25g

04.2CL/ST 3.50

nh tnh/25g

Mu nc

Thc
phm,
nguyn
liu ch
bin thc
phm