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ABBOTT

CELL-DYN 1800
Automated Hematology Analyzer
Service & Support Manual

ABBOTT CD1800 SM
Theory of Operation ............................................................................................................................................................................ 4
System Overview.................................................................................................................................................................... 4
Major Subsystem Descriptions........................................................................................................................................... 6
Circuit Descriptions ............................................................................................................................................................. 15
Signal Processor Module (SPM)....................................................................................................................................... 18
Cell Count Module (CCM) ................................................................................................................................................... 21
Troubleshooting................................................................................................................................................................................. 31
Troubleshooting Charts...................................................................................................................................................... 33
Raw Data Description.......................................................................................................................................................... 40
CCM On-Board Diagnostic LEDs...................................................................................................................................... 41
CPU Hardware/Software Configuration .......................................................................................................................... 44
Service Special Commands............................................................................................................................................... 45
Sample Probe Description ................................................................................................................................................. 49
CELL-DYN 1800 Error Messages...................................................................................................................................... 60
Software Commands and Sequence ............................................................................................................................... 72
Engineering Drawings and Schematics ............................................................................................................................................. 76
CELL-DYN 1800 PCB Reference .................................................................................................................................................... 77
Removal & Replacement .................................................................................................................................................................. 96
Service Equipment Required............................................................................................................................................. 96
Covers (CD18-A1) .................................................................................................................................................................... 97
Flow Panel (CD18-B1)............................................................................................................................................................ 105
Fluid Power Supply (CD18-C1).............................................................................................................................................. 115
Syringe Assembly (CD18-E1)................................................................................................................................................. 119
RR-E1.04 Sample Syringe Driver Assembly .................................................................................................................... 125
RR-E1.06 Lyse Syringe Driver Assembly..................................................................................................................... 127
Electronics / Card Cage (CD18-F1) ........................................................................................................................................ 129
RR-F1.01 PAM (Pre-Amplifier Module) ............................................................................................................................. 129
RR-F1.02 MPM (Motor Processor Module) Board .......................................................................................................... 131
RR-F1.03 CDM (Cable Distribution Module) Board ........................................................................................................ 133
RR-F1.04 Hard Disk Drive................................................................................................................................................... 135
RR-F1.05 Floppy Disk Drive ............................................................................................................................................... 138
RR-F1.06 Signal Processor Module (SPM)...................................................................................................................... 140
RR-F1.07 Cell Count Module (CCM)................................................................................................................................. 142
RR-F1.08 Data Link Adapter (DLA) ................................................................................................................................... 143
RR-F1.09 Single Board Computer (SBC) ......................................................................................................................... 145
RR-F1.10 Card Cage Backplane PCB .............................................................................................................................. 147
LCD/Keyboard (CD18-G1)..................................................................................................................................................... 149
Power Supply (CD18-H1).................................................................................................................................................... 151
Verification Procedures ................................................................................................................................................................... 154
VP-01 Preparation for Alignment and Verification ................................................................................................................. 154
VP-01 Preparation for Alignment and Verification..................................................................................................... 154
Test Equipment and Supplies Required....................................................................................................................... 154
Preparation for Alignment/Verification ......................................................................................................................... 155

Order of Alignment/Verification...................................................................................................................................... 156


VP-02 Decontamination .................................................................................................................................................... 157
VP-03 Vacuum and Pressure Adjustments.................................................................................................................. 158
Regulator Alignment.......................................................................................................................................................... 159
Pressure Adjustment (0.5 psi)......................................................................................................................................... 160
Pressure Verification (High) ............................................................................................................................................ 161
Vacuum Adjustment (8 inch) ........................................................................................................................................... 161
VP-04 Metering System Timing Adjustments - RBC and WBC .............................................................................. 162
Metering Timing Fault Report.......................................................................................................................................... 163
RBC Metering System Timing Adjustment .................................................................................................................. 165
WBC Metering System Timing Adjustment ................................................................................................................. 166
VP-05 CMOS Setup Verification/Adjustment............................................................................................................... 167
VP-06 Card Cage Backplane Test Points ..................................................................................................................... 171
VP-07 Cable Distribution Module Test Points............................................................................................................. 172
VP-08 Pre-Amplifier Module (PAM) Adjustment ......................................................................................................... 173
VP-09 Signal Processor Module (SPM) Verification/Adjustment ........................................................................... 176
RBC Gain .............................................................................................................................................................................. 178
RER Adjustment ................................................................................................................................................................. 179
WBC Gain ............................................................................................................................................................................. 182
PLT Gain ............................................................................................................................................................................... 184
VP-10 Diluent and Sample Verification/Adjustment ........................................................................................................ 187
Diluent Volume Verification ............................................................................................................................................. 188
Sample Volume Verification ............................................................................................................................................ 188
VP-11 Stepper Motor Power Test and Verification .................................................................................................... 189
VP-12 Sample Probe Alignment Check ........................................................................................................................ 190
VP-13 Serial Transmit to LIS Verification..................................................................................................................... 191
VP-14 Hard Disk Drive Setup and Verification............................................................................................................ 194
VP-15 Software Installation/Upgrades .......................................................................................................................... 197

Theory of Operation
System Overview
The CELL-DYN 1800 Automated Hematology Analyzer is a complex system. Analyzer performance depends on several
components that together make up the complete hematology system. The system is comprised of the following components
and subsystems:

Flow Panel System [3]

Fluid Power Supply [2]

Reagent Inlet Panel [6]

Syringe Drive Assemblies [7]

Electronics Card Cage [8]

LCD Display System [4]

Touchpad (Membrane) Keyboard [5]

Power Supply Assembly [1]

Flow Panel

The Flow Panel consists of tubing, solenoid valves, and other hardware components used for sample aspiration, dilution,
measurement and waste removal.
Fluid Power Supply
The Fluid Power Supply contains the vacuum and pressure pumps, accumulators, waste bottles, and associated solenoids
and hardware.
Reagent Inlet Panel
The Reagent Inlet Panel provides connections for incoming reagents and outgoing waste. The Lyse solenoid is also mounted
on this panel.
Syringe Drive Assembies
The Syringe Drive Assemblies include the Sample Syringe for aspirating samples, the Diluent Syringe for supplying Diluent
throughout the Flow Panel, and the Lyse Syringe for dispensing Lyse to the WBC transducer for the HGB measurement
process.
Electronics Card Cage
The electronics card cage, with associated PCBs, provides command and control signals for the various electronic
components of the instrument. This assembly contains the Backplane PCB, Cell Count Module, Signal Processor Module,
Data Link Adaptor and Single Board Computer.
LCD Display Screen and Keyboard
The LCD Display Screen provides a visual data display and the keyboard provides data input by the operator.
Power Supply Assembly

The Power Supply Assembly provides an AC and DC voltage source to various components on the CELL-DYN 1800 System.
The DC Regulator PCB provides power to the Backplane PCB for use with the digital circuitry on various PCBs. It also
provides power to the Cable Distribution Module and fans.
The AC Regulator PCB provides power to the Backplane PCB (for use with the analog circuitry on various PCBs). It also
provides power to the Pump Relay Module.
The +28VDC Switching Power Supply provides power for the stepper motors via the Motor Processor Module. It also
provides power to initially energize solenoids.
Major Subsystem Descriptions
To aid in understanding the overall system, the electronic modules are divided into the following major functional
subsystems:

Data Interface and Control Subsystem

Measurement Subsystem

Solenoid Motor and Pump Subsystem

Single Board Computer Subsystem

AC and DC Power Distribution Subsystem

Data Interface and Control Subsystem


The purpose of this subsystem is to interface the user data, control data, measurement data, and system status data in the
system. This data is connected via four independent data busses:

DLA/CCM (Data Link Adapter/Cell Count Module)

CCM/SPM (Cell Count Module/Sample Processor Module)

CCM/CDM (Cell Count Module/Cable Distribution Module)

CCM/MPM (Cell Count Module/Motor Processor Module)

See the figure below for a diagram showing the data connections.
Data Interface and Control Block Diagram

When power to the instrument is turned ON, the system is operating software is loaded from the hard disk into RAM on the
SBC (Single Board Computer). The SBC then uses various handshaking signals and data bytes to communicate with the
CCM (Cell Count Module) via the DLA (Data Link Adapter).
The CCM functions as the master controller with all system functional commands residing in firmware (PROM). The CCM
sends control data and receives status data from the CDM (Cable Distribution Module).
The CCM provides current control to the von Behrens RBC and WBC Transducers and the two metering PCBs and serves as
the system's analog voltmeter for use in converting the HGB signal. Data is written and read via the CCM/CDM and
CCM/MPM data buses.
The CDM (Cable Distribution Module) acts as a controller for the solenoids and also interfaces data from various system
sensors.
The MPM (Motor Processor Module) acts as a controller for all Stepper Motor Drive PCBs.
Measurement Subsystem

The measurement subsystem provides detection, amplification, and processing of the signals from the von Behrens
RBC/PLT Transducer, von Behrens WBC Transducer, and HGB Flow Cell. RBC/PLT and WBC metering is also included in
this subsystem.
See the figure below for a diagram of the measurement process.
Measurement Block Diagram

The PAM (Pre-Amplifier Module) supplies constant current for the von Behrens RBC/PLT and WBC Transducers and HGB
LED voltage.
The RBC/PLT and WBC cell pulses are input to the PAM where they are amplified and routed to the SPM (Signal Processor
Module).
When the SPM receives signals from the RBC/PLT and WBC, the following occurs:

The RBC/PLT signal is amplified (gain) and split into independent RBC and PLT signals.

The WBC signal is amplified (gain).

The RBC signal is routed to the cell editing circuitry.

Cell editing is performed on the RBC signal to eliminate invalid RBC pulses.

The SPM discriminates cell size by converting pulse height to a proportional digital value. The amplitude of each valid pulse
is measured by a fast A/D converter then sent across the data bus to the CCM.
The A/D data for RBC, PLT, and WBC are individually divided by the CCM into 256 discrete size channels. The cell count in
each channel is accumulated in discrete memory locations and is used to generate count data, percentage data, and
histogram data for RBC, PLT, WBC, and other derived parameters.
Signals from the upper and lower detectors on the RBC/PLT and WBC metering PCBs are converted to TTL levels by
comparators on the CDM. The signals are then routed to the CCM where they are used to control RBC/PLT and WBC
sample timing.
The HGB analog signal from the HGB Flow Cell is captured by the PAM where it is amplified and routed to the CCM. The
HGB signal is then measured and converted to a digital format by a voltmeter-A/D converter.
Solenoid, Motor Drive, and Pump Subsystem
Solenoid control commands reside in firmware on the CCM. These commands are sent to the CDM where they are
multiplexed to the appropriate SDM (Solenoid Drive Module). The SDM then provides the current to open and close
individual drive solenoids.
Stepper Motor commands are handled in much the same manner as described above. However, the final multiplexing of the
Stepper Drive PCBs is controlled by the MPM.
There are two pressure pumps and one vacuum pump in the CELL-DYN 1800 System. These pumps are described as
follows:

A pressure pump provides air to bubble-mix samples in the Pre-Mixing Cup and the mixing chambers of the von
Behrens RBC/PLT and WBC Transducers. A pressure regulator regulates the 0.5 psi in the pressure accumulator for
this process.

An unregulated pressure pump provides air to push waste from the waste bottles inside the instrument to the waste
container attached to the instrument and to apply back pressure to clear the apertures in the von Behrens RBC/PLT
and WBC Transducers.

An 8" Hg vacuum accumulator, vacuum sensor, and vacuum pump supply a constant vacuum to the entire system to
transport Diluent, Detergent, and Lyse throughout the flow system and to maintain a constant vacuum to the
RBC/PLT and WBC metering tubes. A vacuum regulator maintains a constant vacuum source to both metering
tubes.

See Solenoid, Motor Drive and Pump Block Diagram for a diagram of the solenoid and motor drive connections.
Solenoid, Motor Drive and Pump Block Diagram

Single Board Computer Subsystem

The Single Board Computer subsystem consists of the following components:

Single Board Computer PCB

Data Link Adapter

Input/Output Ports (serial/parallel)

Keyboards (PC and membrane)

LCD Display Screen

Disk Drives (hard and floppy)

The figure below illustrates the major components of the User Interface Computer.
User Interface Computer

SBC (Single Board Computer) PCB


The Single Board Computer (SBC) PCB is a complete Celeron 850 Megahertz PC computer system with 128 Megabytes of
RAM that is self-contained on one board. It utilizes a PC compatible BIOS with DOS capability that directly interfaces with the
LCD Display Screen, Hard Disk Drive, Floppy Disk Drive, PS/2 Keyboard, one (1) Parallel Port and two (2) Serial Ports. It
connects directly into the Backplane PCB along with the Data Link Adapter (DLA) and utilizes the ISA bus and Backplane
PCB to communicate with one another.
DLA (Data Link Adapter)

The DLA PCB connects directly into the Backplane PCB. It provides a program-controlled data channel from the SBC to the
CCM. The DLA performs the following three functions:

Receives measurement and analyzer status data from the CCM

Transfers commands from the SBC to the CCM

Sends control data from the UIC to the CCM.

Serial I/O Ports


The CELL-DYN 1800 contains two (2) Serial I/O Ports for transferring data to other computer systems:

COM1 RS-232 Data Output


COM2 Spare

Parallel I/O Port


The CELL-DYN 1800 contains one parallel I/O port for transferring data to a printer.
PC/2 Keyboard
The external 101 key PC keyboard is used to enter alphanumeric data, such as demographic information, into the instrument.
Touch Pad (Membrane) Keyboard
The touch pad (membrane) keyboard is located below the LCD Display Screen. The keyboard includes a row of eight (8)
unmarked rectangular-shaped keys corresponding to labels displayed at the bottom of the screen. These keys activate the
indicated function or display the indicated submenu.
LCD Display Screen (Color)
The LCD Display Screen has the following characteristics:

Size: 8.5 x 6.4 inches (10.4 inches measured diagonally)

Number of colors: 16

Pixels: 640 width x 480 height (or 800 x 600)

Backlight on/off control (software controlled screen saver)

An LCD Adapter, connected directly on the SBC PCB, drives the LCD Display Screen. The adapter supports SVGA 640 x
480 and 800 x 600 graphics modes.
Hard Disk Drive
The hard disk drive stores the User Interface Software program and the Patient Data Log.

Floppy Disk Drive


The 1.44MB 3.5" floppy disk drive is used for program installation and provides the QC (Quality Control) data
upload/download capability.
Printer
The User Interface Software supports Epson ESC-P or PCL-3 languages. The printer has its own buffer and is capable of
printing on 8.5" x 11" (letter size) or A4 paper size. The printer supports alphanumeric and graphics reports from stored data
and screen displays.
Speaker
The PC speaker or buzzer is controlled by software and is mounted on the SBC PCB.
AC and DC Power Distribution Subsystem

The Power Supply Assembly is comprised of three components: AC Regulator PCB, DC Regulator PCB and +28VDC
Switching Power Supply. These components are mounted together as an assembly and are located at the right/rear side of
the instrument.
When the system is turned on, the AC line is routed into the AC Regulator PCB and +28VDC Switching Power Supply. The
AC Regulator PCB automatically accommodates line voltages of 90 - 130VAC and 200 - 260VAC by sensing the input
voltage and utilizing an internal comparator bank and power transformer to produce the 120VAC necessary for the

subsystem's function. The Power Supply Assembly then provides an AC and DC voltage source to various components on
the CELL-DYN 1800 System.
+28VDC Switching Power Supply
The +28VDC Switching Power Supply provides the voltage source to the DC Regulator PCB, which the board then uses to
produce other voltages. The power supply cooling fan is thermistor controlled, which means that when the internal
temperature rises above 70C, the fan operates at full speed. The fan is then turned off at 50C and kept off until the
temperature rises above 70C.
AC Regulator PCB
The AC Regulator PCB provides the 120VAC used by the Pump Relay Module (PRM) for vacuum and pressure pump
operation.
The 12VDC (analog) is provided to the Backplane PCB, which is used by the Cell Count Module (CCM) and Signal
Processor Module (SPM). The 12VDC is also provided to the Cable Distribution Module (CDM), Motor Processor Module
(MPM) and Pre-Amplifier Module (PAM) (for its circuitry).
The +100VDC is provided to the PAM for use in its constant current circuitry. In between the AC Regulator PCB and the PAM
is the Pre Amp Filter that is used to filter out noise.
DC Regulator PCB
The DC Regulator PCB provides +5VDC, 12VDC (digital), +14VDC and +28VDC. The +5VDC is provided to the Backplane
PCB, which is used by the SPM, CCM, Data Link Adapter (DLA) and Single Board Computer (SBC). This voltage is also used
by the CDM, MPM, Hard and Floppy Disk Drives.
The 12VDC (digital) is provided to the Backplane PCB for use on the CPU fan. The Hard and Floppy Disk Drives receive
+12VDC for their operation.
The +14VDC is provided through the CDM to the Solenoid Driver Modules (SDM) and is used to hold the solenoids closed or
open for normally closed solenoids.
The +28VDC is provided to the MPM for operating stepper motors and through the CDM to the SDMs to initially close
solenoids or open for normally closed solenoids. This voltage is also used for the system's internal cooling fans. The cooling
fans are thermistor controlled, which means that when the ambient temperature inside the instrument drops below 25C, the
fans operate at half speed. Once the temperature rises above 25C, the speed is increased linearly until it reaches 35C,
when the fans operate at full speed.
The DC Regulator PCB also provides +5VDC and +14VDC to the AC Regulator PCB.
Circuit Descriptions
This section contains a description of the circuitry for the following printed circuit boards:

Pre-Amplifier Module (PAM)

Signal Processor Module (SPM)

Cell Count Module (CCM)

Cable Distribution Module (CDM)

Solenoid Driver Module (SDM)

Motor Processor Module (MPM)

Stepper Drive Printed Circuit Board (SDP)

Pressure/Vacuum Regulator Module (PVRM)

Power Supply Assembly

Pump Relay Module (PRM)

Single Board Computer (SBC)

Data Link Adapter (DLA)

Pre-amplifier Module (PAM)


Note
Refer to PAM PCB Diagram.
PAM PCB Diagram

The PAM performs the following functions:

Provides constant current control to the von Behrens RBC/PLT and WBC Transducers.

Amplifies the initial RBC/PLT, WBC and HGB signals.

The constant current bias (+100VDC) is switched by U6, then routed to Q2 and Q3, which supplies constant current to the
von Behrens RBC/PLT transducers. The RBC/PLT current is adjusted with R72. Once received, the RBC/PLT signals are
initially amplified by U7, then routed to U5, where they are re-inverted and further amplified. The combined RBC/PLT signal is
then routed to the SPM PCB.
Transistors Q4, Q5 and associated circuitry provide constant current for the von Behrens WBC Transducer. The WBC
current is adjusted via R66. Once received, the WBC signal is initially amplified by U12, then routed to U11 where it is
re-inverted and further amplified. The WBC signal is then routed to the SPM PCB.

The output of the HGB Flow Cell is amplified by U1 and U2 prior to being routed to the SPM PCB. The HGB self test and gain
voltages are adjusted with R14 and R4 respectively.
Signal Processor Module (SPM)
Note
Refer to SPM Architecture.
SPM Architecture

EPLD (SPM)
The EPLD is an enhanced programmable logic device. The SPM EPLD is used to control data acquisition.
There are three state machines that run in the EPLD. One each is used for controlling data acquisition elements such as
Multiplexers and Peak/Hold Amplifiers. The inputs to the EPLD state machines are the outputs of the various Threshold and
Slope Detectors as well as Bubble and Area Comparators.

There is a data transfer state machine which controls data flow to the CCM.
Signal Processing
There are two signals from the RBC and WBC transducers that are processed through two basic data acquisition circuits.
The Platelet signal is derived from the RBC signal. There are three gain adjustments associated with each of these signals.
In addition to the three gain adjustments there is a integration adjustment for detecting pulses that are too long.
WBC Signal

The WBC signal is received through a differential amplifier to reduce noise. There is a gain adjustment after the
differential amplifier but before the Test Signal injection point.

The WBC signal is then "Baseline Restored" to remove DC components and Baseline fluctuations due to varying
duty cycle of the blood cells.

A threshold detector signals to the EPLD the presence of valid pulses.

Simultaneously the Bubble detector signals if the pulse is determined to be a bubble. The bubbles are much larger
than pulses from blood cells. The EPLD discards the information from the pulse if it is determined to be a bubble.

The data from the pulse is processed on the falling edge of the Threshold Detector unless the Slope Detector senses
another rising edge before the falling edge of the Threshold Detector. The data is processed immediately if there is
another rising edge before the falling edge of the Threshold Detector.

The WBC Held Peak is converted and sent to the CCM on a 15S cycle. Average pulses from the impedance
transducers are 35S.

RBC and PLT Signals


There are two modes for RBC/PLT data acquisition:

RBC MCV

RBC Count/PLT

RBC MCV
RBC MCV is for RBC MCV data only. In this mode, an integrator is enabled to determine if the cell is moving through the
middle of the aperture. If the cell is not moving through the center of the aperture, the data is falsely high and the pulse is
abnormally long. The integrated signal is compared to a proportion of its height. If the pulse is too long for its height, it is
discarded.
RBC Count/PLT
RBC Count mode is for count information. During this run, Platelets are counted simultaneous with RBCs and no integration
is used. The Platelet signal is derived from the RBC signal by an adjustable gain.

The RBC signal is received through a differential amplifier to reduce noise. There is a gain adjustment after the
differential amplifier but before the Test Signal injection point.

The RBC signal is then "Baseline Restored" to remove DC components and Baseline fluctuations due to varying duty
cycle of the blood cells. At this point an additional gain stage is added to create the Platelet signal.

A threshold detector(s) signals to the EPLD the presence of valid pulses. The Platelet Threshold Detector is used if in
RBC Count mode.

If in Count mode, the Platelet Lower and Upper Threshold detectors are used. If the Upper Threshold Detector is
triggered, the pulse is a RBC and the RBC pulse is accumulated into the RBC Count histogram. If only the Lower
Detector is triggered, the PLT pulse is accumulated into the PLT histogram.

The data from the pulse is processed on the falling edge of the Threshold Detector unless the Slope Detector(s)
senses another rising edge before the falling edge of the Threshold Detector. If this occurs, the data is processed
immediately.

The RBC Held Peak or the PLT Held Peak is converted and sent to the CCM on a 15s cycle.

Cell Count Module (CCM)


Note
Refer to PAM PCB Diagram.
CCM Architecture

System Clock and Microprocessor Description


The CCM uses a 16 MHz crystal as a time base. The fundamental cycle time for the MC68HC11KW1 processor is 4 MHz.
The processor has many built in functions such as:

16 bit address bus

8 bit data bus

7 - 8 bit multi-purpose I/O ports (CCM and SBC ports)

Small amounts of Internal RAM and Internal EEPROM (analyzer serial number)

Internal Timers (system timer)

Internal UART (debug port)

EPLD (CCM)
The EPLD is an enhanced programmable logic device and is used for address decode and histogram building functions. Its
program is used in coordination with the processor software.
LEDs
Note
Refer to CCM On-Board Diagnostic LEDs.
Scratch Pad RAM
This RAM is used for general purpose programming.
Histogram Memory
This RAM is used for histogram building.
Histogram Building (cell counting)
The following sequence is executed to build histograms:
1. Histogram Memory is cleared.

2. Counting is enabled.
This disconnects Histogram Memory from the processor buss.
3. Wait for an End of Conversion (EOC) signal from SPM.
4. At the EOC, data is read from the SPM ADC
5. The data then becomes the address for the histogram memory.

6. The data from the histogram memory is read.


This data is the count information for the respective pulse height.
7. The count is incremented and stored back into histogram memory.

8. Counting is disabled. And the histogram can be read by the processor.


HGB Measurement
A 12 bit ADC with an input multiplexer is used to measure the HGB signal from the PAM. This data is read by the processor.
Along with the HGB measurement, various DC voltages are read by the 12 bit ADC as a diagnostic.

CDM Port
The CDM port is connected directly to microprocessor ports E, G and K.
SBC Port
The SBC port is connected directly to microprocessor ports J and part of port A.
Cable Distribution Module
Note
Refer to Solenoid, Motor Drive and Pump Block Diagram.
Solenoid, Motor Drive and Pump Block Diagram

The CDM performs the following functions:

Status Sensor Interface

Control of Solenoid Driver Module

Pump Relay Module interface and control

Start Board (Touch Plate) Interface

The CDM communicates with the CCM via the CCM/CDM data bus at J2. Analog outputs of the Metering Modules are
converted to TTL levels by comparators (U12) and placed directly on the CCM/CDM data bus. Signals from the Pump Relay
board, Probe Position Switches, and Start Board (Touch Plate) are interfaced by Data Drivers (U5, U10).
Data is interfaced to the Solenoid Driver Modules via J32. This data is then multiplexed by One-of-Eight Decoders (U1, U2)
via J3, J4, J6, J7, and J9.
Vacuum and pressure control data is latched by U14 and routed to the Pump Relay Module via J11. Pump status signals
(Vac On, Pres On) are converted to TTL levels by U3 and placed on the data bus by U5.
LED drive signals are routed to the Start Board (Touch Plate) via J17. The start signal enters at J17 and is placed on the data
bus by U5.
Solenoid Driver Module (SDM)
Note
Refer to Solenoid, Motor Drive and Pump Block Diagram.
The purpose of the SDM is to provide drive current to the solenoids. Each SDM has eight Darlington drivers (Q1-Q8) which
are individually controlled by data bits (D0-D7) and data latch (U3).
There are two power modes available for each solenoid - activate (+28V) and hold (+14V). This is controlled by the Hi CLK
signal in conjunction with data bits (D0-D7) and the current control latch (U1).
Motor Processor Module (MPM)
Note
Refer to Solenoid, Motor Drive and Pump Block Diagram.
The MPM controls drive data to the Stepper Drive printed circuit boards and also provides self-test capability for motor
winding current. The MPM is comprised of the following major circuits:

Microprocessor

Program Control EPROM

I/O Peripheral Interface Adapter (PIA)

Direct Memory Access (DMA) Control

Motor Phase Latches

Motor Current Latches

Motor Winding Self Test

Control functions of the MPM are performed by microprocessor (U5).

The operating program for the microprocessor is stored in Program Control EPROM (U3).
Data communications between the CCM and MPM are controlled by I/O PIA (U6) and serial data is interfaced via ACIA (U2)
and Data Bus Connector (J1).
Phase data, motor direction, and step rate are stored in RAM (U7). This data is sent to the Motor Phase Latches under
control of the DMA Control circuitry, which consists of U11, U12, U15, U16, U18, U21 and associated circuitry. The data is
strobed into the appropriate Motor Phase Latch by ALG0 through ALG2.
The Motor Phase Latches U23, U26, and U29 provide phase data to the Stepper Drive printed circuit boards. Each is an 8-Bit
Addressable Latch which can control up to four Stepper Drive printed circuit boards and subsequently four Stepper Motors.
Four levels of motor current for each motor is controlled by the Motor Current Latches (U22, U25, and U28). Each latch can
control up to four stepper drive printed circuit boards. Data is strobed into the appropriate latch by WR0 through WR2.
The Feedback- and Feedback+ inputs at J3 through J14 are connected, via resistors on the Stepper Drive printed circuit
board, to the stepper motor windings. This allows the circuitry consisting of U30, U31, and U32 to monitor the winding current
during an internal self-test. These values can be read by the CCM to isolate a defective Stepper Drive or Stepper Motor.
Stepper Drive Printed Circuit Board
Note
Refer to MPM section, blocks 20, 23, and 24 of Solenoid, Motor Drive and Pump Block Diagram.
The Stepper Drive printed circuit board consists of two PBL 3717 motor drive chips. Each chip drives a winding of the
Stepper Motor. Bits I0 and I1 are used to control four motor current levels:

P0 - High Current

P1 - Medium Current

P2 - Low Current

P3 - Current Off

Bits PH0 and PH1 control motor phase and, therefore, direction and step-rate (velocity). Feedback+ and Feedback- are used
to generate a motor self-test.
Pressure/Vacuum Regulator Module
Note
Refer to Solenoid, Motor Drive and Pump Block Diagram.
Pressure (or vacuum) is sensed by a transducer that is internally configured as a Wheatstone Bridge. Transistor Q1and
resistors R4 and R5 are used to generate a stable reference voltage for the Wheatstone Bridge. The output of the
Wheatstone Bridge is partially amplified (U1-7), stabilized against long term drift (voltage follower U1-1) and made
offset-adjustable by R18 and associated resistors.

Maximum transducer sensitivity can only be achieved when the output is zero volts at TP-1 and when there is no pressure
differential across the transducer. To accomplish this, R18 is adjusted for zero volts when both transducer inlet ports (P1 and
P2) are open to atmospheric pressure.
In order to maintain the operating point of comparator U2-14 at the fixed 2-volt trip level, it is necessary to maintain the output
of U2-8 within a relatively narrow range. This is accomplished by making the differential amplifier (whose inputs are U1-10
and U2-10) adjustable by selecting 1 of 4 possible jumper positions. A stable reference point for the DC operating level of
U1-8 and U2-8 is established by U1-14 in conjunction with R6 and resistor network RP1.
Measurement of pressure in the range of approximately 0.5 lbs/sq. inch is accomplished by using transducer inlet port P1 in
conjunction with jumper setting A/B.
Vacuum pressure in the range of 8 inch Hg is accomplished by using inlet port P2 in conjunction with jumper setting C/D. The
regulation point for either vacuum or pressure is established by the setting of potentiometer R16.
When the output of comparator U2-14 goes positive, the collector of Darlington transistor Q2 is pulled to ground, thereby
turning on either the pressure or vacuum pump. When the pumps are running, LED DS1 is lit and stays lit until either the
pressure or vacuum increases past the hysteresis point established by R8 of U2-14.
The output of the pressure/vacuum regulator can be inhibited by a logic low at J1-1. This completes the circuit description.
Power Supply Assembly
Note
Refer to major subsystem descriptions of AC and DC Power Distribution Subsystem.
Pump Relay Module (PRM)
Refer to Solenoid, Motor Drive and Pump Block Diagram.
The PRM provides drive to the vacuum and pressure pumps, via three Solid State Relays: K1, K2, and K3.
Single Board Computer (SBC)
The Single Board Computer (SBC) is designed around a Celeron 850MHz microprocessor and connects directly into the
Backplane PCB. The SBC receives power from the Power Supply Assembly via the Backplane PCB and receives status and
measurement data from the CCM through the DLA. The board contains EPROM, CMOS, RAM, input/output circuitry for the
interface ports, disk drives (hard and floppy) circuitry, LCD Display Screen Interface, and PS/2 Keyboard connectivity.
Data Link Adapter (DLA)
The Data Link Adapter provides interfaces from the SBC to both the CCM (Cell Count Module) and the Touch Pad
(membrane) keyboard. These two interfaces function independently under user interface software control. However, some of
the circuits on the DLA board are shared. The DLA uses an 82C55 PPI (Parallel Peripheral Interface) IC. Refer to the figure
below for an illustration of the DLA board.

DLA (Data Link Adapter) Block Diagram

Interface to CCM
A 20-pin ribbon cable connects the DLA to the CCM. This interface is a bi-directional, parallel interface that is
software-controlled at both ends. Data is transferred in 8-bit bytes on 8 data lines (D0 - D7) in one direction at a time. The
SBC (Single Board Computer) initiates an inquiry message handshake on a periodic basis or when it has a command to send.
The CCM responds by sending data or replying to the command sent.
The interface is controlled cooperatively by the UIC and CCM according to the state of the handshake signals. Refer to Cell
Count Module (CCM). The UIC always sends data first. The CCM responds by sending data back (if any is available). Thus,
the 82C55 switches its A-port from input to output and back to input during every message transaction.
The key handshake signals are REQ1 and REQ2. REQ2 going low initiates the communication protocol. The CCM responds
by bringing REQ1 low. After the DLA has sent its data, it brings REQ2 high. Then the CCM sends its data.

Each byte received by the DLA generates an IRQ. The application software responds to the IRQ by putting the byte into a
buffer. When the CCM brings REQ1 high, the communication is complete; the DLA returns to an idle state, and port A is set
to input. Both REQ1 and REQ2 remains high until the next message/data transfer.
The two LEDs on the printed circuit board, DS1 and DS2, indicate the active state of REQ2 and REQ1, respectively. They
should always be flickering when the application software is running because the User Interface Software program is
constantly polling the CCM to check its state. (There are some exceptions to this during power up and certain diagnostics/test
modes.)
The I-O address of the DLA board is assigned by four jumpers. The default I-O address (pins 3 - 6 on S1 hard-wired) is: [off
off on off], 340 hexadecimal. The DLA interrupt level is assigned by a jumper.
Jumpers W1 - W6 assign the DLA interrupt to one of the following:

W#

IRQ #

W1

IRQ 5 *

W2

IRQ 10

W3

IRQ 10

W4

IRQ 11

W5

IRQ 12

W6

IRQ 15

* Since IRQ 5 is used by the DLA, W1 is hard-wired (this is the default).

Interface to Touch Pad (membrane) Keyboard


A 14-pin ribbon cable connects the DLA to the key panel. The key panel is also polled by the User Interface Software
program. This interface is not interrupt-controlled.
The keyboard is interfaced as a parallel switch matrix circuit with 4 lines out and 8 lines in. Four scan rows are driven active
one at a time via an LS175 latch. Then the columns of the key panel matrix are read from port B of the 82C55. The software
interprets a low active signal as a key is pressed.
The DLA board must be plugged into a 16-bit slot. There are no adjustments on the DLA board.

Troubleshooting
Diagnostics Menu Usage
Utilization of the DIAGNOSTICS Menu enables the operator and/or service representative to identify and correct both
operator-correctable and service-correctable faults. When the computer senses a fault, the message <NOT READY: SEE
DIAGNOSTICS> displays in the System Status Box. The following keys are available in the DIAGNOSTICS Menu.
Level One

INITIALIZATION: Used to perform an Initialization cycle: returns movable components to home position and
performs internal self-tests.

RAW DATA: Used to display raw measurement data for the last specimen.

COUNT TEST: Used to run specimens without returning to RUN Menu and display Raw Data.

MORE: Used to display additional functions (levels).

PRINTER OUTPUT: Used to toggle printer output ON and OFF.

HELP/ERROR: Used to display help information regarding the diagnostics menu screens. The fault log can also be
accessed through this key function.

MAIN: Used to return to Main Menu.

Level Two

WBC HISTOGRAM: Used to display WBC count and histogram data accumulated in each of 256 size channels.

RBC HISTOGRAM: Used to display RBC histogram data accumulated in each of 256 size channels.

PLT HISTOGRAM: Used to display PLT count and histogram data accumulated in each of 256 size channels.

SMOOTHING ON/OFF: Used to toggle histogram display status. With Smoothing Off, only raw counts are displayed.
With Smoothing On, channels are numbered, data is normalized and the number of the peak channel displays.
Smoothing On/Off (example) gives an example of Smoothing On/Off.

MORE: See Level One description.

PRINTER OUTPUT: See Level One description.

HELP/ERROR: See Level One description.

MAIN: Used to return to Main Menu.

Level Three

PROBE HOME: Moves Sample Probe up and above RBC Cup. Displays probe assembly sensor status.

PROBE UP: Moves Sample Probe up. Displays probe assembly sensor status.

MORE: See Level One description.

PRINTER OUTPUT: See Level One description.

HELP/ERROR: See Level One description.

MAIN: Used to return to Main Menu.

Level Four

SYSTEM STATUS: Used to display all pending alarms.

FAULT REPORT: Used to display all pending faults or warnings.

SERVICE HEX CODES: Hex system codes. Not used for operator or service troubleshooting.

SERVICE DEC CODE: Used to initiate individual actions in the CELL-DYN 1800 hardware and software.

MORE: See Level One description.

PRINTER OUTPUT: See Level One description.

HELP/ERROR: See Level One description.

MAIN: Used to return to Main Menu.

Smoothing On/Off (example)

Fault Report Description


A detailed list of all faults generated by the CELL-DYN 1800 System software and hardware is contained in CELL-DYN 1800
Error Messages. The fault classifications reported in the Fault Report primarily contains data pertaining to the last CCM fault.

If a fault occurs, pressing the [HELP/ERROR] key immediately displays the [FAULT LOG] in the DIAGNOSTICS Menu. This
log may contain up to 16 faults, with the most current fault at the top of the list. An alternative procedure is to go to the MAIN
MENU and press [DIAGNOSTICS]. In this case, the [FAULT REPORT], not the Fault Log, is immediately displayed.
The Fault Log can be viewed from any of the menus, except SETUP. To view the Fault Log, enter the desired menu, followed
by [HELP/ERROR] and [FAULT LOG]. The system displays up to 16 past faults. From the MAIN MENU, press
[DIAGNOSTICS] followed by [MORE] three (3) times and [FAULT REPORT] to display the FAULT REPORT screen. A
display of <NO FAULTS OR WARNINGS PENDING> indicates that all faults have been cleared.
CELL-DYN 1800 Troubleshooting Guide
A list of symptoms, probable causes, and corrective actions for the most common problems encountered on the
CELL-DYN 1800 System is given in the Troubleshooting Chart. The probable causes and corrective actions for each
symptom are arranged in descending order from most likely to least likely. When troubleshooting a problem, start with the
most likely cause first.
If possible, thoroughly verify that a component is defective before replacement. Some problems can be verified visually, but
other problems may require a measurement tool such as a DVM (Digital Volt Meter).
When troubleshooting DATA PROBLEMS, only the measured parameters RBC, PLT, WBC, HGB, and MCV should be used
for reference. Using the calculated parameters can become confusing when trying to isolate a problem.
When troubleshooting CLOG AND FLOW ERROR PROBLEMS, refer to VP-04 Metering System Timing Adjustments - RBC
and WBC for the MIN and MAX specifications for the RBC and WBC Upper (T1) and Lower (T2) times.
Troubleshooting Charts
Nonfunctional Instrument Problems

Symptom

No Functions. No Fans

Probable Cause

Corrective Action

Power Cord

Check Power Cord

Power Source

Check Power Source

No 5VDC

Check that 5VDC LED on Card Cage Backplane is On


Note
If off replace Power Supply Assembly.

No Functions. Fans Run

Video Display Problems

Defective CCM

Replace CCM

Defective SBC

Replace SBC

Symptom

LCD Display Screen

Probable Cause

Corrective Action

Loose Connection

Check all LCD Display Screen Connections

Flash BIOS or

Connect a computer monitor to the top of the SBC PCB. Power ON

CMOS settings

instrument and open CMOS setup screen. Check that Advanced

reset to Default

Chipset Setup is configured correctly. Refer to VP-05 CMOS Setup

Blank/Solenoids OK

Verification/Adjustment.

Note
Do not measure voltage
on backlight inverter

Defective Backlight Replace Backlight Interconnect PCB


Interconnect PCB

PCB. Measuring voltage

Defective LCD

can damage PCB.

Interconnect PCB
Defective LCD

Replace LCD Interconnect PCB

Replace LCD Display Screen

Display Screen
Characters Out Of Focus

Defective LCD

Replace LCD Display Screen

Display Screen
Defective SBC

Replace SBC PCB

PCB
Characters OK But Garbled

Defective LCD

Replace LCD Display Screen

Display Screen
Defective SBC

Replace SBC PCB

PCB
Missing Characters

Defective LCD

Replace LCD Display Screen

Display Screen

Displayed Error and Fault Problems

Symptom

Probable Cause

Restriction

Corrective Action

Check Lines
Check In-Line Sensor

Detergent Empty

Defective In-Line Sensor

Replace Sensor PCB

Defective CDM

Replace CDM

Solenoid 3-1, 3-3, 3-4 Tubing

Check Solenoid 3-1, 3-3, 3-4

Diluent Empty

Restriction

Check Lines
Check In-Line Sensor

Defective In-Line Sensor


Defective CDM

Replace Sensor PCB


Replace CDM
Check Solenoid 3-3

Solenoid 3-3

Replace Solenoid 3-3


Check Solenoid 3-4

Solenoid 3-4

Replace Solenoid 3-4


Check Solenoid 3-1

Pressure Overlimit
Solenoid 3-1

Replace Solenoid 3-1


Check Pressure Switch

Defective Pressure Switch

Replace Switch

Defective CDM

Replace CDM

Leak 8" Hg

Check for air leaks in Fluid Power Supply and


Flow Panel

Vacuum Low Error

Defective Pump

Replace Pump

Defective Vac Regulator

Replace Vacuum Regulator

Leak 0.5 Psi

Check for air leaks in Fluid Power Supply and


Flow Panel

Pressure Low Error

Defective Pump

Replace Pump

Defective Pres Reg

Replace Pressure Regulator


Check Unreg Pressure

Waste Overflow Into


Accumulators

No Air Pressure

Replace Unreg Pump


Replace CDM
Check Solenoid 5-3

Solenoid 5-3

Replace Solenoid 5-3


Check Solenoid 5-7

Solenoid 5-7

Replace Solenoid 5-7


Check Solenoid 1-6

Solenoid 1-6

Replace Solenoid 1-6

Check Accumulator Sensor Connections


Accumulator Sensor Falsely Detecting Flush Accumulator with DI Water
Liquid
Replace CDM
Check Internal Waste Sensor Connections
Defective Internal Waste Sensor

Replace Sensor
Check Unreg Pressure

Waste Empty Timeout

Unreg Air Pressure Low


Restriction

Replace Pump
Check tubing at Fluid Power Supply, Waste
Bottles, A and B

CCM/DLA Communication Error

Defective CDM

Replace CDM

DLA/CCM Cable connection

Check DLA/CCM Cable connection

Defective DLA/CCM Cable

Replace DLA/CCM Cable

Defective CCM Board

Replace CCM Board

Defective DLA Board

Replace DLA Board

Defective SBC PCB

Replace SBC PCB

Power Source

Check 5VDC and +12VDC (Digital) at Card Cage


Backplane PCB
Check CMOS Setup

Disk Errors (Hard Disk or Floppy


Drives)

Defective SBC PCB


Defective Disk Drive (Hard Disk or

Replace SBC PCB


Replace Disk Drive

Floppy Drives)
Defective Switch

Replace Switch and Perform Alignment

Defective CDM

Replace CDM
Run Motor Power test

Defective Stepper Drive Printed


Circuit Board

Replace Chopper Drive PCB

Position Faults
Exercise Probe (Diagnostic Menu, Probe Home)
Defective Sample Probe Assembly

Replace Sample Probe Assembly


Run Motor Power Test

Defective Motor

Replace Motor

Data Problems

Symptom

Probable Cause

Corrective Action

Check 12VDC (Analog) LEDs on Card Cage Backplane


PCB
All Results Are "0"

No 12VDC (Analog)

Replace Power Supply Module


Replace CCM
Check that PAM 100VDC LED is On

No 100VDC
HGB OK All Others "0"

Replace Power Supply Assembly

Defective SPM

Replace SPM

Defective PAM

Replace PAM

Reference lower than

Check for bubbles in Detergent line and Solenoid 2-6

sample reading
No sample aspiration

Check associated tubing, solenoids and sample syringe


Perform VP-08 Pre-Amplifier Module (PAM) Adjustment

HGB "0" all others OK

Defective PAM

Replace PAM

Defective CCM

Replace CCM

Defective HGB Flow Cell

Replace HGB Flow Cell


Check 0.5 psi (Bubble Mix) pressure

0.5 psi (Bubble Mix)

Check associated tubing and hardware

pressure
Perform Pressure Adjustment (0.5 psi)
Inadequate Probe Cleaning
Imprecision on all Parameters

Perform Sample Volume Verification


Imprecise Sample Aspiration

Imprecision on all Parameters,


HGB OK

Check associated wash block tubing and hardware

Check associated sample aspiration tubing and hardware

Imprecise Diluent Dispense

Perform Diluent Volume Verification

Carryover

Check for correct draining of Pre Mix Cup

Defective SPM

Replace SPM

Defective PAM

Replace PAM

Dirty RBC/PLT Transducer

Clean RBC/PLT Transducer

Imprecision on RBC and PLT,


WBC/HGB OK

Check 0.5 psi (Bubble Mix) Pressure


Incorrect 0.5 psi (Bubble

Check associated tubing and hardware

Mix) Pressure
Perform Pressure Adjustment (0.5 psi)
Defective SPM

Replace SPM

Carryover

Check for correct draining of RBC/PLT Transducer

Dirty WBC Transducer

Clean WBC Transducer


Check 0.5 psi (Bubble Mix) Pressure

Incorrect 0.5 psi (Bubble


Imprecision on WBC/HGB,

Check associated tubing and hardware

Mix) Pressure
Perform Pressure Adjustment (0.5 psi)

RBC and PLT OK


Defective SPM

Replace SPM

Carryover

Check for correct draining of WBC Transducer

Dirty Flow Cell

Clean Flow Cell


Check reference reading (Diagnostics Menu, Raw Data)

Reference reading out of


Imprecision on HGB, Others OK specification

Perform HGB Ref Adjustment on PAM VP-08 Pre-Amplifier


Module (PAM) Adjustment

Defective Flow Cell

Replace Flow Cell

Solenoid operation

Check Solenoid 3-6, 2-6 and 2-7

Dirty Aperture.

Clean RBC/PLT Transducer and Aperture Plate

RBC RER

Perform VP-09 Signal Processor Module (SPM)

Imprecision on MCV

Verification/Adjustment
Defective SPM

Replace SPM

Dirty Transducer

Clean WBC Transducer and Aperture Plate


Replace Lyse Syringe

Incorrect Lyse Volume


WBC "R" Codes, Reagents OK

WBC Gain

Replace Lyse Syringe Drive Assembly


Perform VP-09 Signal Processor Module (SPM)
Verification/Adjustment
Replace SPM

Defective PCBs

Replace CCM
Check power

High Electrical Backgrounds

Environmental

Check overhead lighting


Check for other items on same power line

Install filter (line conditioner)


Isolate line (dedicated line)
Check grounding cable on Front Cover
Poor instrument cover

Replace grounding cable

grounding
Check EMI Shielding on Card Cage Backplane PCB
Check correct routing of WBC and RBC/PLT Transducer
cables to PAM
Defective PAM
Replace PAM
Check Power Supply Module
"Noisy" Power Supply
Module

Replace Power Supply Module


Check Connections on Pre-Amplifier Filter PCB

Defective Pre-Amplifier Filter


PCB
Defective Transducer(s)

Replace Pre-Amplifier Filter PCB


Replace Transducer(s)

Clog and Flow Error Problems

Symptom

Probable Cause

Corrective Action

Perform VP-03 Vacuum and Pressure Adjustments


Incorrect vacuum
Diluent and Detergent lines
"CLOG" both sides constant

Check Solenoid 1-4 and 1-5 (closed during metering)


Check Reagent lines

reversed
Metering Tube position

Top of Metering Tube (tapered edge) should be visible on


top of upper DET sensor
Check Vent Tubing

"CLOG" T1 = Max T2 = 0, No Vent Restriction


Dirty Transducer
"CLOG" T1 = Max T2 = 0, No
Meniscus

Check Vent Solenoid 3-6, 2-1 and 1-3


Clean Transducer and Aperture Plate
Check plumbing

Restriction

Check Count Solenoid 1-2 and 4-3


Check Vent Solenoid 1-3 and 2-1

Check for bubbles on right side of Transducers


Dirty Transducer

Clean Transducer and Aperture Plate


Check associated tubing

"CLOG" T1 = Max T2 = 0, Slow

Restriction

Check Vent Solenoid 3-6

Meniscus
Perform VP-03 Vacuum and Pressure Adjustments
Incorrect Vacuum

"CLOG" T1 = Max Meniscus


speed OK

"CLOG" T1 =OK T2 = Max


Meniscus speed OK

"FLOW ERR" T1 = Min T2 = Max

"FLOW ERR" T1 = OK T2 = Min

Check Solenoid 1-4 and 1-5 (closed during metering)

Defective Upper Detector

Replace Metering Printed Circuit Board

Defective CDM

Replace CDM

Defective Lower Detector

Replace Metering Printed Circuit Board

Defective CDM

Replace CDM

Defective Upper Detector

Replace Metering Printed Circuit Board

Defective CDM

Replace CDM

Defective Lower Detector

Replace Metering Printed Circuit Board

Defective CDM

Replace CDM

Miscellaneous Problems

Symptom

Garbled Histograms

Probable Cause Corrective Action

Defective SPM

Replace SPM

Defective CCM

Replace CCM

Raw Data Description


From the MAIN MENU, press [DIAGNOSTICS] followed by [RAW DATA]. The [RAW DATA] key displays raw data obtained
from the last count cycle.
When a single count is done, all data is contained in the first column. When a PLT recount occurs, data from the first cycle
displays in column #2 and data from the recount displays in column #1.
Raw Data Display Description

RBC, WBC and PLT counts are RAW, uncorrected total counts.

HGB Error is not used.

HGB Reference is the output of the A/D Converter when reading reference (2000 = 5 volts).

HGB Sample is the output of the A/D Converter when reading sample (2000 = 5 volts).

WBC and RBC Up Times are the upper times in milliseconds for the last sample.

WBC and RBC Count Times are the times in milliseconds for the last sample.

Flow Error is coded Clog or Flow Error data.

RBC RER is RBC Cell Editing percentage.

WBC and RBC Upper max and Upper min are the maximum and minimum Upper Times, respectively.

WBC and RBC Avg. Time are the averages of the previous count times. The WBC and RBC Avg. time is reset when
the [Clear Orifice] key is pressed.

WBC and RBC Time-Outs are the floating Upper Clog Alarm Limits calculated by the "Running Average Program".
Note
An example of a raw data report displays in Raw Data (example).

Raw Data (example)

CCM On-Board Diagnostic LEDs


The seven LEDs on the CELL-DYN 1800 CCM can reveal much about the fundamental CCM and overall machine state. In
general, the LEDs indicate whether the CCM is in a normal functioning mode or in a fault state, and in either case, help to
characterize the CCM state. Also, LED2 gives some information about the state of the SBC.
The CCM tests itself on power-up. These fundamental tests include MC68HC11KW1, RAM, and SPM interfaces. If any test
fails, the CCM attempts to execute an endless loop routine which flashes the green LED on the board. Also, it places a 4-bit
fault code into the adjacent yellow LEDs (see Power-on LED Patterns - Fault States on Startup).
LED Function & Counting Control

The LEDs on the CCM (labeled LED1 through LED7) are entirely under program control. Their use is as follows:
Note
Refer to CELL-DYN 1800 CCM for location of LEDs on PCB.

LED7, a green LED, should not be not flashing after the CCM has successfully completed its internal power-on
self-check diagnostics, otherwise if it is flashing slowly (~1Hz.), there is a fundamental CCM fault.

LED5 and LED6, for CER and CEW, indicate the state of the CCM firmware generated signals CER (Count Enable
Red) and CEW (Count Enable White). These signals enable cell counting. When the associated LED is on, the DMA
cell counting circuitry is active.

LED3 and LED4, for WCP and RCP, are on during the metered count time intervals, LED3 for the white count time
interval and LED4 for the red count time interval.

LED2 is driven by the signal NREQ1, and indicates the UIC/CCM communication activity. This LED state directly
relates to the hi/lo state of NREQ1. When LED2 is flickering, which should always be the case during normal system
operation, it indicates that the UIC/CCM communications link is active. (There is a corresponding LED on the DLA
(UIC comm. board) that will also be flickering in sync with LED2; it is controlled by the DLA output signal NREQ2.)

LED1 is used to indicate that a self-test is in progress. This indicates that the tests for the pulse processing A to D
circuitry on the SPM with the pulse counting circuitry on the CCM are active.

Power-on LED Patterns - Normal Setup

LED2

LED3 LED4

LED5

LED6

LED7

Step

State

NREQ1

WCP

RCP

ON

ON

ON

CEW

CER

READY

ON

Power on
RAM testing

Flashing

Flashing

4
5

Flashing

Flashing

RBC/PLT testing
WBC testing

ON

ON

CCM tests done; Homing flowscript running

ON

ON

UIC program comm. started


Initialized (running/idle)

Power-on LED Patterns - Fault States on Startup

LED2

LED3 LED4 LED5 LED6

LED7

CCM

NREQ1 WCP

ON

RCP

CEW

CER

READY

Fault

ON

Flashing MC68HC11KW1 CONFIG reg

ON

Flashing MC68HC11KW1 RAM test

ON

Flashing 8K RAM bit test


Flashing 8K RAM clear test

ON

ON

ON

ON

ON

Flashing MC68HC11KW1 timer test


ON

CELL-DYN 1800 CCM

Flashing Histogram RAM test

Flashing CDM init. test

Cell Count Monitor (CCM) PCB

J6 - NOT USED

S1 RESET

LEDs 2-7

S3 BUFFALLO

10

E2 DGND

J2 MPM

11

J8 - NOT USED

J1 CDM

12

E3 DGND

J3 SPM

13

E1 DGND

J5 DLA

14

LED 1

CPU Hardware/Software Configuration


RS-232 Communications Test Procedure
Detailed information on the CELL-DYN 1800 System Interface Specifications is available on the WWCS Intranet website.

CMOS Setup
The CMOS Setup contains all the information needed by the Basic Input/Output System (BIOS) to establish proper
communications between the single board computer (SBC) and the various computer system devices. Refer to VP-05 CMOS
Setup Verification/Adjustment:
Special Function
Probe Check
There are two probe check functions activated by softkeys in the DIAGNOSTICS menu.

The [PROBE UP] softkey moves the probe up and maintains position without rotational motion. (The [PROBE
DOWN] softkey then displays to restore the probe to the down position.)

The [PROBE HOME] softkey places the probe over the RBC/PLT transducer. (The [PROBE DOWN] softkey then
displays to restore the probe to the down position.)
Note
Neither procedure puts the probe in the STANDBY position (on the left).

Service Special Commands


Discussion
Several commands are available to initiate individual actions in the CELL-DYN 1800 System hardware and software. These
commands are used for troubleshooting and/or alignment when a single action is desired or required to be repeated several
times.
The special command mode resides in the DIAGNOSTICS Menu. From the MAIN Menu, press [DIAGNOSTICS] followed by
[MORE] three (3) times, then [SERVICE DEC CODE]. When this softkey is pressed, the message Test Select --- FOR
SERVICE USE ONLY --- displays.
A command can now be entered. Pressing the Enter key on the keyboard initiates the action. Only one command can be
entered at a time and [SERVICE DEC CODE] must be pressed before a command is entered.
All commands available by direct softkey can be accessed by pressing [MORE].
Note
Use only the commands listed in DEC Service Commands and always verify that the correct number has been
entered before initiating the action. Use only those numbers listed in DEC Service Commands. Other numbers may
refer to engineering commands which are not used in the field and which may cause damage if used improperly. Be
fully aware of the purpose of any of the DEC Service Commands before using them. This is a direct-activation
method which should be used with caution because the physical state of the CELL-DYN 1800 System may not be in
agreement with the function to be performed. After using service commands, always re-initialize the system by
turning the power OFF then ON again or by pressing the [INITIALIZATION] key in the DIAGNOSTICS Menu to
ensure the instrument is in the proper configuration for normal operation.

DIAGNOSTICS Menu Service Code Function List


When the [SERVICE DEC CODE] key is pressed, the (Enter number (currently, 102):____ prompt displays.
The number above corresponds to the decimal code for the last code entered.
DEC Service Commands lists the decimal-coded (DEC) service commands that can be invoked by pressing the [SERVICE
DEC CODE] key in the DIAGNOSTICS Menu and entering the appropriate number.
DEC Service Commands

UIC DEC Codes

Function

07

NOT USED

08

NOT USED

09

NOT USED

11

NOT USED

15

fill lyse into system

16

NOT USED

17

NOT USED

18

NOT USED

19

fill Diluent & detergent

20

mini-wash

22

NOT USED

23

NOT USED

24

NOT USED

25

NOT USED

26

NOT USED

33

NOT USED

34

NOT USED

36

NOT USED

37

pre-dilute sample run setup

38

pre-dilute sample run exit

39

aperture current off (uses whole blood script)

40

open all valves

41

NOT USED

47

platelet recount

48

initialization (homing)

49

open sample run

50

clean orifice (back-flushing)

51

pre-dilute sample run

52

background count run

53

prime system with all reagents

54

daily shutdown

55

empty transducers and cups

56

gain adjust

57

unpinching normally closed valves

59

fill transducers and cups after empty

60

gain adjustment setup

61

dispense 10 ml saline

62

open sample wash

63

clean-for-shipping

64

clean sample syringe setup

65

aspirate 40 l sample for 1/250 dilution

66

dispense 10 ml saline for 1/250 dilution

67

aspirate 100 l sample for 1/50 dilution

68

dispense 5 ml for 1/50 dilution

69

NOT USED

71

lyse syringe down

72

NOT USED

73

NOT USED

74

lyse syringe up and home

75

lyse syringe down restore

76

pre-dilute sample wash

77

NOT USED

78

NOT USED

81

NOT USED

83

diluent syringe down

84

enzyme clean setup

85

probe up and rotate and home

86

back to ready position from probe home

87

probe up for probe adjustment

88

probe down (when finished, operator should initialize the instrument to place the probe in the home
position)

89

sample syringe up and restore

90

sample syringe down and home

91

enzyme clean the system

92

diluent syringe up and home

93

diluent syringe down and restore

117

NOT USED

118

NOT USED

119

NOT USED

120

NOT USED

121

cycle solenoids on waste assy

122

cycle solenoids on flow panel assy

123

sample syringe aspirate

124

sample syringe dispense

125

vacuum test

126

check mixing pressure

127

check backflush pump

128

motor power test (see Service DEC Code 128)

129

motor power level test (see Service DEC Code 129)

130

exercise motors (see Service DEC Code 130)

999

auto-cycle (see Auto-Cycling (Code 999))

Note
Certain commands are not sent to the CCM when the system is in an interlock state, such as STANDBY or
UNINITIALIZED.

Auto-Cycling (Code 999)


The CELL-DYN 1800 can be pre-set to do a specified number of RUN cycles without user intervention. This capability
applies only to normal RUN Count Test, Pre-Dilute RUN, (PRE-DIL TEST), Gain Adjust (GAIN ADJ), and Electrical
Background (ELEC BKGD). This capability helps reduce test time for the instrument. The following entry screen displays
after entering code 999:
-- Auto Cycle Test Set Up -Use Spacebar to accept current number
Use "<--" key to delete a digit
Use "ESC" key to cancel
Enter Number of Times to Repeat Test (currently, 10):
Sample Probe Description
The motors that enable the Sample Probe to move up/down and to rotate are stepper motors which are under direct
computer control. Since there is no direct positional feedback sent to the computer, position switches are employed to verify
critical positions during normal operation. It is important to understand that these switches only verify and do not control the
movement of the Sample Probe.
In the DIAGNOSTICS Menu, Service DEC Codes 128, 129, and 130 allow the service representative to control and exercise
all stepper motors in the CELL-DYN 1800 System. This description focuses on the Probe Up/Down Motor (B/2) and the
Probe Rotate Motor (C/3) which control the movement of the Sample Probe.
Service DEC Codes 128, 129, and 130 Descriptions
These commands reside in the SERVICE DEC CODE screen of the DIAGNOSTICS Menu and are used to test, control, and
exercise CELL-DYN 1800 stepper motors. A description of each of these three commands is given.
Service DEC Code 128

This code runs a computer generated test (Motor Power Test) of all stepper motors, motor driver boards, and associated
circuitry.
The Motor Power Test should be run whenever a problem is suspected with any assembly that is driven by a stepper motor.
The following entry screen displays after entering code 128:
Motor Power Test Started.
To MPM: {I }
To MPM: {pD32}
To MPM: {mC1!2} AC}
To MPM: {C1}
inp: 0415
A report (Motor Power Test (example)) automatically displays and can be printed. Refer to VP-11 Stepper Motor Power Test
and Verification.
Note
Press the [INITIALIZE] key before leaving the DIAGNOSTICS Menu.
Motor Power Test (example)

Service DEC Code 129

This code allows the Run and Idle power levels to be set when exercising a stepper motor. The four levels are:
0) Full Power
1) Medium Power
2) Low Power
3) Off
This code tests mechanical assemblies at various power levels or to remove idle power so the mechanism can be more
easily moved or checked manually. The Motor Power Level Test (example) screen displays after entering code 129 (press
the ENTER key after each entry):
Motor Power Level Test (example)

Note
After the entries are made, a message, such as Motor "A" set to running power of 1 and idle power of 3,
displays.
Service DEC Code 130
This code allows the direction, speed, and number of steps to be set when exercising a stepper motor.
The Motor Check (example) screen displays after entering code 130:
Motor Check (example)

Note
After the entries are made, a message, such as Motor "B": motion in direction "0" at speed "&" for 100 steps,
displays.
Motor Direction Commands
The table below contains information on the motor designation, command and direction of the motor to be tested. Motor
Speed Commands lists the motor speed commands to determine the speed of the motor being tested. Both tables are
needed to properly test the motor.
Motor Direction Commands

Motor Designations

A/1

B/2

C/3

D/4

Function

Sample Syringe

Probe Up/Down

Probe Rotation

Diluent Syringe

Command

Direction

Down/Aspirate

Up/Dispense

Up

Down

CCW/To RBC cup

CW/To Pre-Mixing Cup

Down/Aspirate

Up/Dispense

CCW/Dispense

E/5

Directional Valve

F/6

Spare

G/7

Spare

H/8

Lyse Syringe

Motor Speed Commands

Command Speed in Steps per Second

50

75

283

300

166

200

250

10

151

10

222

11

25

12

182

13

100

14

125

15

91

16

67

17

111

Sample Probe Normal Operation

CW/Aspirate

Down/Aspirate

Up/Dispense

Probe Up/Down INITILIZE and RUN Modes illustrates the Sample Probe's up/down sequence during the INITIALIZE and
RUN cycles. Probe Rotate "INITALIZE" Mode shows the probe's rotation movement during the INITIALIZE cycle.
Probe Up/Down INITILIZE and RUN Modes

Probe Rotate "INITALIZE" Mode

Initialization Mode

The Initialization cycle places mechanical and electrical components in the "home" position, drains any liquid in the tubing,
Pre-Mix Cup, and the Mixing Chamber of the von Behrens RBC Transducer to the Waste System, then places the instrument
in the INITIALIZED state.
Stepper Motor Homing
Homing a stepper motor is the process of setting up the initial position from which all future movement is referenced. In the
CELL-DYN 1800 System, this is accomplished by forcing the motor to move against a physical stop (Hard Stop). When the
mechanical assembly, driven by the motor, reaches the Hard Stop, the stepper motor electrically slips until it is forced to stop.
This mechanical position then becomes the zero reference position for the motor.
Operation:
1. The Sample Probe moves up at a fast speed until the Upper Switch (#2) is activated. It is then changed to a slow
speed, and homed against the Upper Hard Stop, which is the metal plate at the top of the Sample Probe Assembly.
2. The probe moves down six steps and the Upper Switch (#2) is checked.
3. The probe moves CCW at a fast speed until the Right Switch (#4) is activated. It is then changed to a slow speed,
and homed against the Right Hard Stop, which is the mounting bracket for Right Switch (#4).
4. The probe moves CW to the Pre-Mix Cup and Left Switch (#3) is checked. The probe then moves into the Pre-Mixing
Cup.
5. The probe moves up and Upper Switch (#2) is checked.
6. The probe moves CCW to center and down positions; and the Lower Switch (#1) is checked.

7. This completes the Initialization cycle.


Run Mode
The figure below illustrates the probe's movements during the RUN cycle.
Probe Rotate "RUN" Mode

Operation:
1. When the Start Switch is pressed, 30 L of sample is aspirated and Lower Switch (#1) is checked.
2. The Sample Probe then moves up to a position six steps from Upper Hard Stop, and Upper Switch (#2) is checked.
3. The probe moves CW to Pre-Mix Cup and Left Switch (#3) is checked.
4. The probe moves down eight steps and into the Pre-Mix Cup, where dispense, probe shake, and aspiration of RBC
sample takes place.
5. The probe then moves up to a position six steps from Upper Hard Stop, and Upper Switch (#2) is checked.
6. The probe moves CCW to the Mixing Chamber of the von Behrens RBC/PLT Transducer, stops three steps from
Right Hard Stop, and Right Switch (#4) is checked.
7. The probe moves down into the RBC/PLT Mixing Chamber and RBC sample is dispensed.
8. The probe moves up to a position six steps from Upper Hard Stop, and Upper Switch (#2) is checked.
9. After completion of the count cycle, the probe moves CW to center position.
10. The probe moves down and Lower Switch (#1) is checked.

11.This completes the RUN cycle.


Switch Failure Descriptions
Example of fault reports are shown in the following figures:

Lower Switch (#1) Fault Report


Upper Switch (#2) Fault Report
Left Switch (#3) Fault Report
Right Switch (#4) Fault Report

When a switch is checked by the computer and found to be deactivated (open) in normal operation, the message "Not Ready:
SEE DIAGNOSTICS" displays on the RUN Menu.
From the MAIN MENU, press [DIAGNOSTICS]. The screen immediately displays one of the Fault Reports shown in Lower
Switch (#1) Fault Report, Upper Switch (#2) Fault Report, Left Switch (#3) Fault Report, and Right Switch (#4) Fault Report.
The message <SWITCH: 1 CHECK> indicates that Lower Switch (#1) failed when checked. The message <* NOT ON ANY
SWITCH *> indicates that none of the switches were activated when the failure occurred. Refer to Lower Switch (#1) Fault
Report.
Lower Switch (#1) Fault Report
The message <SWITCH: 2 CHECK> indicates that Upper Switch (#2) failed when checked. The message <* NOT ON ANY
SWITCH *> indicates that none of the switches were activated when the failure occurred. Refer to Upper Switch (#2) Fault
Report.

Upper Switch (#2) Fault Report


The message <SWITCH: 3 CHECK> indicates that Left Switch (#3) failed when checked. The message <ON SWITCH(ES):
2 TOP> indicates that Left Switch (#3) was activated when the failure occurred. Refer to Left Switch (#3) Fault Report.

Left Switch (#3) Fault Report


The message <SWITCH: 4 CHECK> indicates that Right Switch (#4) failed when checked. The message <ON SWITCH(ES):
2 TOP> indicates Right Switch (#4) was activated when the failure occurred. Refer to Right Switch (#4) Fault Report.

Right Switch (#4) Fault Report


The above conditions do not necessarily indicate that a switch has actually failed. They only indicate that the switch was not
read as activated when checked by the computer. A failure could also be caused by improper switch alignment, an electronic
hardware failure, or a mechanical hardware failure.

CELL-DYN 1800 Error Messages


The table below lists error messages on the CELL-DYN 1800 System.

Description

Error Message
(Status box)

Time-out at N seconds

A CCM process initiated by the user took longer to complete than allowed (usually indicating
a failure of the CCM). The process ran approximately N seconds before the time-out
occurred.

Process Aborted

A count test was stopped either by the user or because of a fault detected by the CCM.

Fix then press [CLEAR

A user-correctable fault condition was detected.

ALARM]
Process Monitoring Aborted

A process was stopped by the user (using the asterisk (*) key.

Error Message

Description of the error.

(Display Area II)


Printer Time-out

The Printer Output option was ON and the printer did not print the requested report in the

expected time.
Code N is Invalid

The user has entered a command for the CCM whose numeric value exceeds 127. The
value entered was N.

Cannot do this Function

The user has attempted to issue a command to the CCM that cannot be executed because
of a pending fault condition.

WBC Meniscus Detection,

During the most recent count, a meniscus was not detected or was detected at an

RBC Meniscus Detection

unexpected time.

WBC Count Time-out (clog),

During the most recent count, a Clog occurred.

RBC Count Time-out (clog)


CCM Pulse Height Memory

During the most recent count, there was an overflow in one of the pulse-height arrays

Saturation Warning

(histograms).

External Waste Full

The external waste bottle has been filled.

Lyse Empty, Detergent

The indicated reagent has run out.

Empty, Diluent Empty


Invalid Alarm Set

A bit was set in the fault message from the CCM that has no valid interpretation.

*NOT ON ANY SWITCH*

After some mechanical motion, a reading of all the position sensors indicates that none are
activated. (This message does not necessarily mean that a mechanical fault has occurred.)

Waste Overflow Into

A reading of the sensor in the Waste Accumulator suggests that there is liquid in the

Accumulators

accumulator.

Error Message (Status box)

Description

Vacuum

There was a vacuum failure during power-up or the instrument is unable to maintain vacuum
level while in the READY state.

Pressure

There was a pressure failure during power-up.

Position Fault

A mechanical assembly is not in the correct position for the most recent function to be
performed, as indicated by position sensors.

Sensor Fault - Internal Waste

A Time-Out fault occurred in draining one of the waste bottles. This error is also associated

Empty

with positive pressure.

Canceling Auto-Cycling

This message displays when the user cancels Auto-Count Testing.

Invalid UIC Command Sent to The UIC sent a command to the CCM that it cannot interpret.
CCM
Error in Flow System Timing

An error in the timing of a flow script has occurred. This occurs during instrument
initialization when the flow script takes more than three minutes to complete.

Histogram Memory Clear

The CCM was unable to clear the pulse-height memory.

CCM Program, RAM Memory

The CCM detected a failure in its RAM.

CCM/MPM Message Fault


Other error-related messages
MPM to CCM, Message
Transmit Error
Command to be Sent to MPM
is Incorrect

An error in CCM/MPM interprocessor communications occurred. A fault was generated in an

Abnormal Time-out/No MPM

attempt to send or receive motor or other command to or from MPM, or the MPM was unable

Response

to perform the function.

Fault Response from MPM


Incorrect Command to be
Sent to MPM
Attempt to Send MPM a New
Command while Busy
No Such Script in ROM or

Error in loading a flow script.

RAM
CCM/UIC Message Fault

An error in UIC/CCM interprocessor communications occurred.

No Response from CCM

The CCM is not functioning or the signal cable connecting the CCM and UIC is faulty or
disconnected. Turn the instrument OFF, check the CCM/UIC cable, then turn the instrument
ON.

CCM is Initializing

The CCM is in the middle of its Initialization process.

Undefined Event

An undefined event or process occurred.

Count Test
The [COUNT TEST] key in the DIAGNOSTICS Menu is used to run specimens and display Count Check data without
returning to the RUN Menu. Coded data relating to specific cycle functions, raw measurement, and flow count time are
displayed for use in troubleshooting or service.
Event Messages During Diagnostic Menu Count Test lists the event messages that are displayed during the Diagnostic Menu
Count Test.
Event Messages During Diagnostic Menu Count Test

Event Messages

Description

SampSw. pressed

Touch Plate was pressed.

Remove specimen

Specimen should be removed.

Count valve open

The counting valve is open.

RBC histogram avail RBC histogram is available.


WBC upper det

WBC upper meniscus detection.

WBC lower det

WBC lower meniscus detection.

RBC upper det

RBC upper meniscus detection.

RBC lower det

RBC lower meniscus detection.

Plt recount strt

Platelet recount starts.

CCM initing

CCM initializing.

Data invalid

Data entered is invalid.

WBC histo avail

WBC histogram is available.

Proc complete

Process is completed.

Data avail

Data is available.

CCM init done

CCM initialization is completed.

Canceled

Canceled operation.

Operator-Correctable Alarm or Fault Messages


This table lists operator-correctable alarm or fault messages.

Event Messages

Description

External Waste Full Waste full sensor is activated.


Detergent Low

Detergent is low as detected by reagent sensor in reagent inlet tube.

Diluent Low

Diluent is low as detected by reagent sensor in reagent inlet tube.

Lyse Empty

No lyse is detected by reagent sensor in reagent inlet tube.

Invalid alarm set

Incorrect error or message was set.

Detergent Empty

No detergent is detected by reagent sensor in reagent inlet tube.

Diluent Empty

No diluent is detected by reagent sensor in reagent inlet tube.

CCM is initializing

CCM module is currently in the INITIALIZED state.

Summary of Error Messages


Summary of Error Messages gives a complete listing of error messages available on the CELL-DYN 1800 System.
Summary of Error Messages

Error Message (Status box)

DOS Errors
Arithmetic overflow
Bad drive request structure length
Cannot remove current directory
Cannot rename across drives
Collection index out of range
Collection overflow
CRC error in data
Device read fault
Device write fault
Disk full
Disk is write-protected
Disk read
Disk seek
Division by zero
Drive not ready
File access denied
File not assigned
File not found
File not open

File not open for input


File not open for output
Floating point overflow
Floating point underflow
Hardware failure
Heap overflow
Invalid drive number
Invalid file access code
Invalid file handle
Invalid floating point operation
Invalid function number
Invalid numeric format
Invalid pointer operation
Path not found
Printer out of paper
Range check
Sector not found
Stack overflow
Too many open files
Range check
Sector not found
Stack overflow
Too many open files
Unknown command
Unknown media type
Unknown unit

General
Demographic entry disabled
No data to print

Stopping
Unable to Load Flowscript
Unable to Save Cal Factors
Unable to Set Volume

System Fault
Not Ready: See DIAGNOSTICS
Uninitialized

Normal Operator-Correctable
Detergent empty
Diluent empty
Lyse empty
Waste full

DLA Faults
<-? Invalid error code
CCM command echoed does not match
CCM REQ1 high during data send at char
CCM REQ1 is stuck LOW
DLA buffer overflow on receiving char
DLA/CCM error on command
Incorrect checksum on CCM data receive
NAK received from CCM
No response from CCM
Time-out 1 on wait for CCM REQ1
Time-out 2 on message send to CCM
Time-out 3 on message receive from CCM

Date

Battery failure or system date/time not set

Disk I/O
Cannot open CD1800.ini configuration file
Cannot read CD1800.ini configuration file
Cannot write to CD1800.ini configuration file
CD1800.ini configuration file size error
CD1800.ini configuration file version error
Configuration file error
Error when reading CRC values from disk
Memory error when creating CRC tables

Ticket Printer
Data err
Inc. asp.
No Ticket Detected

QC Log
Can not accept specimen
Can not reject specimen
No loading, QC file has to be empty
Purge log failed
Read QC file failed

Data Log
Cannot do if uninitialized
Cannot write Data Log header to disk
Count overrange
Data Log write error
Failed to read from Data Log

Flow err

Initialize
Data Log initialization failed
QC Log initialization failed

Communications
Break interrupt
Comm open error
Comm error
Framing error
Parity error
Re-transmit started
Re-transmit time-out
Receiver overrun
Transmit started
Unable to re-transmit

Printer Driver
Printer Fault
Printer Not Ready
Printer Off-line
Printer Out Of Paper
Printer Time-out
Ticket Printer Not Ready

QC Log I/O
Failure to read from QC Log
Failure to write to QC Log
QC Log size error

QC Log version error, bytes short for creation of QC Log

Help
Unable to open help file

Reagent Log
Unable to create file
Unable to open file

Data Log Errors


Cannot Access Data Log
CRC Reading Failed
Data Log initialize failed, bytes short for creation of Data Log
Data Log Print Error
Data Log size error, Call Technical Support
Data Log version error
Data Transmission Error
Not enough memory error

X-B File
Failed to write to Data Log Header
Failed to write to Data Log
No response from CCM

Data Log I/O


Data Log write failure
Write Header failure
Write version error

General Faults

Abnormal time-out / no MPM response


Attempt to send MPM a new command while busy
CCM pulse height memory saturation warning
CCM is in Fault State
CCM is in Unknown State
CCM Program, RAM Memory Fault
CCM real time clock has failed
CCM/DLA Message Communication Fault
Command to be sent to MPM is incorrect
Count Overrange
Count time-out (clog)
Data Capture Fault
Detected Probe Assembly Switch: # in Incorrect State
Detergent sensor
Diluent sensor
Error in Flow System Timing
Fault response from MPM
Guard electrode voltage warning
Histogram Memory Clear Failure
Incomplete Aspiration
Incorrect command to be sent to MPM
Initial Communication with DLA Failed
Invalid Command Sent to CCM
Invalid Data
Mechanical Position Fault
MPM to CCM, message transmit error
No response from MPM
Press [INITIALIZATION] key to clear fault and re-initialize meniscus detector: "True" sensed at metering start meniscus: not
detected during valid time interval
Pressure Level Time-out

Pressure Over-Limit Detected


Printer is not Ready: Cannot RUN uninitialized
RBC Clog
RBC Flow Error
Run time error: # at:
Time out after approximately __ minutes
Turn off instrument and drain accumulators manually
Unexpected response from MPM
Unknown MPM/CCM fault
Vacuum Level Time-out
Waste Drain to Empty Time-out
Waste Overflow into accumulators
WBC Clog
WBC Flow Error
Wrong software for this instrument

Fault Log
Error in writing to Fault Log file
Error in writing header to Fault Log file

Fault Log I/O


Allocating heap memory error
Bytes short to create the Fault Log
Fault Log file size error
Fault Log Header IO Error
Incorrect version of Fault Log

Lab ID Setup
Drive A is not ready
Fail to read assay file

Fail to write LAB ID file


Incorrect assay format
Incorrect disk. QC values do not apply to this instrument
Incorrect Exp. Date
Incorrect Lot Number
Incorrect parameter in assay file

Software Commands and Sequence


CD1800 File and Directory Structure

Accessing DOS
There are two ways to access the DOS program from the CD1800 program:

With the CD1800 program still loaded,

By exiting the CD1800 to DOS. The Exiting from CD1800 to DOS method is the preferred method.

Switching from CD1800 to DOS


1. With the CELL-DYN instrument ON, go to the DIAGNOSTICS Menu.

2. Press ESC on the PC keyboard. The following message displays on the screen.

CD1800 Program Exit


It is recommended that the Cell-Dyn be put in Stand-by before exiting the program. Otherwise, turn the
instrument off after doing any service work while in DOS.
3. Press the Alt and d keys simultaneously. The DOS prompt C:\CD1800> displays.

4. Execute the desired DOS commands. Most commands can be executed. However, computer memory is limited
since the CD1800 program is still loaded.
Exiting from CD1800 to DOS
1. With the CELL-DYN instrument ON, go to the DIAGNOSTICS Menu.

2. Press ESC on the PC keyboard. The following message displays on the screen.
CD1800 Program Exit
It is recommended that the Cell-Dyn be put in Stand-by before exiting the program. Otherwise, turn the
instrument off after doing any service work while in DOS.
3. Press the Alt and x keys simultaneously. The CD1800 program is removed from computer memory. The DOS
prompt (C:\1800>) displays.

4. Execute the desired DOS commands.


Exiting DOS
To exit the DOS program and return to the CD1800 program in the case where the CD1800 program remains loaded
(Alt and d was used to access DOS), type exit at the C:\> prompt and press the Enter key. The DIAGNOSTICS Menu
displays.
To exit the DOS program in the case where the CD1800 program is not loaded, (Alt and x was used to exit the CD1800
program), turn the instrument OFF then ON again to reinitialize the system.
Note
Turning the instrument OFF then ON again prepares the system for normal operation.

Common DOS Commands

Function

Change drives
Change directory
Copy

Command

c: or a:
cd
copy

Description

switch C and A drives


filename
source to destination

Delete

del

file name(s)

Directory

dir

current or specified drive

Exit

exit

quits the current program

Undelete

undelete

file name

DOS Command Usage


Change Drives
When accessing DOS from the CD1800 program, the C:\> prompt displays, indicating the C drive is selected. To change to
the A drive (floppy disk), type A: and press Enter. The prompt changes to A:\> indicating the A drive is selected.
Change Directories/Files Within a Drive
To move from a directory to the root directory (C:\) type cd\ and press the Enter key.
To move one level closer to the root directory type cd.. (where .. are two periods) and press the Enter key. For example, to
move from CD1800\HELP to CD1800 subdirectory type cd.. and press the Enter key.
To move one level away from the root directory type cd_[directory name] (where _ indicates a space) and press the Enter
key. For example, to move from the root directory (c:\) to the CD1800 directory type cd cd1800 and press the Enter key.
Copy
To copy a file on the CELL-DYN 1800 hard drive (C drive) to a floppy disk (on the A drive):
1. Exit the CD1800 program procedure above. Change to A drive, if the file to be copied is on A drive.
2. If necessary, use the cd command to access the directory containing the file(s) to be copied.

3. At the C:\> prompt, type Copy_C:\[filename]_A:\ (where [filename] is the complete file name and _ indicates a
space between characters).
Example: to copy file CONFIG.SYS from the C drive to the A drive, type copy c:\config.sys a:\ and press the Enter
key.
To copy this file from the A drive to the C drive, type copy a:\config.sys c:\ and press the Enter key.
Delete a File
To delete a file:
1. Access DOS (Exiting from CD1800 to DOS). Change to A drive if the file to be deleted is on A drive.
2. If necessary, use the cd command to access the directory containing the file(s) to be deleted.

3. At the C:\> prompt, type delete_ [filename] (where [filename] is the complete file name and _ indicates a space
between characters) and press the Enter key. For example, to delete a file called Sample from the C drive type
delete sample and press the Enter key.
Undelete a File
To undelete a file which had previously been deleted:

1. At the C:\> prompt, type undelete_ [filename] (where [filename] is the complete file name and _ indicates a space
between characters) and press the Enter key. For example, to undelete a file called Sample from the C drive, type
undelete sample and press the Enter key.
View Files in a Directory
To view all the files in a particular directory:
1. Access DOS (Exiting from CD1800 to DOS). Change to A drive if the files to be viewed are on A drive.
2. If necessary, use the cd command to access the directory containing the file(s) to be viewed.

3. At the C:\> prompt type dir and press the Enter key.
Note
If there are many files, the file names scroll down the screen too rapidly for the user to see. The user can prevent this
by adding "switches" to the DIR command using the format: DIR_[switch] (where the underscore ("_") indicates a
space). For example, to display the file names a screen at a time, type dir_/p and press the Enter key. To display the
file names in columns across the width of the page: type dir_/w and press the Enter key.

Engineering Drawings and Schematics


CD1800

CELL-DYN 1800 PCB Reference


AC Regulator PCB

AC Regulator PCB

14

J1

J2

15

J3

TP6

16

J8

TP7 (+14V)

17

LD3 (+14V)

TP5 (VR)

18

LD4 (+5V)

TP4 (GND)

19

J5

R22

20

LD1 (+12V)

J4

21

LD2 (-12V)

J7

22

TP2 (GND)

10

TP9 (GND)

23

TP3 (+12V)

11

TP8 (+100V)

24

TP1 (-12V)

12

R28

25

J6

13

J9

Backlight PCB

Backlight PCB

CN1

CN2

Backplane PCB (front)

Backplane PCB

LD4 (+12V Digital)

J2

LD5 (-12V Digital)

SBC

LD2 (+12V Analog)

DLA

10

LD3 (-12V Analog)

CCM

SPM

11

LD1 (+5V)

Backplane PCB (rear)

Backplane PCB

TP3 (-12V Analog)

J1

TP2 (+12V Analog)

TP6 (DGND)

J10

TP1 (+5V)

10

J9

TP5 (-12V Digital)

11

J11

TP4 (+12V Digital)

CCM PCB

CCM PCB

J6

S1 (RESET)

LEDs 2-7

S3 (BUFALLO)

10

E2 (DGND)

J2 (MPM)

11

J8

J1 (CDM)

12

E3 (DGND)

J3 (SPM)

13

E1 (DGND)

J5 (DLA)

14

LED1

CDM PCB

CDM PCB

18

J25

TP1 (GND)

19

J26

J11

20

J27

J12

21

J28

J13

22

J29

J14

23

J30

J15

24

J31

J16

25

J10

J17

26

J9

10

J18

27

J8

11

J19

28

J7

12

J32

29

J6

13

J20

30

J5

14

J21

31

J4

15

J22

32

J3

16

J23

33

J2

17

J24

34

J1

DC Regulator PCB

DC Regulator PCB

J1

LD2 (+14V)

10

J11

J5

11

LD3 (+12V)

J7

12

J8

J6

13

J9

J4

14

J10

J2

15

LD1 (+5V)

J3

DLA PCB

DLA PCB

DS2 LED

J1

J2

TP1

TP2

DS1 LED

Indicator PCB

Indicator PCB

LD2 (yellow)

LD1 (green)

LD3 (red)

Internal Waste Empty PCB

Internal Waste Empty PCB

J1

E2

J2

E1

LCD Interconnect PCB

1 LCD Interconnect PCB

J2

2 J8

J3

3 J7

JP1B

4 J5

JP1A

5 J6

10 J4 (USED FOR CELL-DYN 1800)

MPM PCB

MPM PCB

TP4 (+28V)

DS1 thru DS4 LED'S

TP2 (GND)

TP1 (GND)

TP6 (+12V)

TP3 (RESET)

10

J2

J3 thru J14

11

J1

TP7 (+5V)

PAM PCB

PAM PCB

13

TP3 (GND)

J3

14

JMP3

J4

15

R14

JMP1

16

J5

J1

17

R72

TP7

18

LD3

R4

19

TP5 (GND)

TP1

20

R66

JMP2

21

TP6 (GND)

10

TP2

22

LD1 (-12V)

11

J2

23

LD2 (+12V)

12

TP8 (GND)

24

TP4 (+100V)

Pre-Amp Filter PCB

Pre-Amp Filter PCB

-12V

+12V

GND

PRM PCB

PRM PCB

J1

J5

J2

J8

J3

J7

J4

J6

10

F1

RBC Metering PCB

J1

RBC Metering PCB

TP2

J1

TP3

TP1

Reagent Empty Sensor PCB

Reagent Empty Sensor PCB

J1

E1

E6

E2

E5

E3

E4

SBC PCB

SBC PCB

LPT1

Fan

COM1

Power

COM2

Hard Disk Drive

PC Keyboard

Floppy Disk Drive

10

LCD Display Screen

SDM PCB

SDM PCB

DS1 thru DS8 LEDs

TP2 (DGND)

J1 thru J8

J9

J10

TP1 PWR GND

SPM PCB

SPM PCB

E2 (AGND)

R22 (WBC)

J1

R27 (RBC)

J4

R41 (PLT)

E1 (DGND)

R51 (RER)

10

E3 (AGND)

Touchpad PCB

Touchpad PCB

J1

J2

Vacuum/Pressure Regulator PCB (front)

Vacuum /Pressure Regulator PCB

J1

P2

DS1

P1

R18

TP3

TP2

TP1

10

E1

Vacuum/Pressure Regulator PCB (rear)

Vacuum /Pressure Regulator PCB

WBC Metering PCB

R16

WBC Metering PCB

J1

TP1

TP3 (GND)

TP2

Removal & Replacement


Service Equipment Required
Every system subassembly and module can be removed and replaced using the tools listed below. Substitute equipment
having the same characteristics as those listed can also be used. Whenever possible, choose non-magnetic tools to avoid
damaging sensitive printed circuit board mounted components and magnetic media.

Required Tool Recommended Model

Description

Cable Ties

Self-locking

Flashlight

Pen light

Knife

Utility

Nut driver

1/4"

8-inch

Nut driver

1/2"

8-inch

Pliers

5"

Diagonal Cut

Pliers

8"

Long Nose

Screwdriver

Phillips No. 2

18-inch

Screwdriver

Phillips No. 1

Stubby

Screwdriver

Phillips No. 2

Stubby

Screwdriver

Slotted 3/16 X 10

8-inch

Screwdriver

Slotted 3/16 X 8

8-inch

Screwdriver

Hex 7/64"

8-inch

Spare tubing

Silicone

Wrench

Various lengths
Adjustable

Covers (CD18-A1)

Removal
Action

Steps

Reference

1. Grasp under the lower right side [2] and

Remove
Upper Front

open the cover [1] toward the left.

Cover
Note
Cover is hinged on the left side and is
kept closed with a magnetic latch.
2. Disconnect cable connector J1 [3] on
the Status PCB [4].
3. Disconnect the ground wire [5] from the
instrument chassis.

4. Use a slotted screwdriver to remove the


C clip [6] from the lower hinge.
Note
Be careful when removing the C clip.
The hinge contains a plastic washer [7]
and a spring [8] which can be lost.
5. Remove the plastic washer and spring.

6. Lift the cover from the hinge pegs and


remove it from the instrument.

Replacement
Action

Install Upper Front Cover

Steps

1. Install in reverse order of disassembly.

Reference

Verification
Action

Steps

Reference

Verification of Proper Cover

1. Place instrument into READY mode.

Installation

2. From the MAIN Menu, press RUN followed by SPECIMEN TYPE, then
NORMAL BACKGRND.

3. Press the touch plate to run a cycle.


Note
During the cycle, make sure that cover does not interfere with
instrument operation.

4. Once cycle is complete, verify acceptable background results. Refer to


Specifications & Checklist.

Removal
Action

Open Upper

Steps

1. Open the upper front cover.

Front Cover
Check

Caution

Sample

Be sure the sample probe is out of the

Probe

way before attempting to remove the

Position

lower front cover to prevent damage to


the probe.

If the instrument is ON, from the MAIN


MENU press [SPECIAL PROTOCOLS]
then [MORE] followed by [PROBE
HOME] to place the probe over the
RBC/PLT Mixing Chamber.

If the instrument is OFF, make sure the


Sample Probe is raised all the way (if not,
turn the lead screw to raise the probe) so

Reference

that it clears the lower front panel.

Remove

1. Remove the thumbscrew [2] from the

Lower Front

lower front cover (to the bezel).

2. Slide the lower front cover [1] to the left

Cover

approximately 1/2-inch until it clears the


bezel, then lift up and out.
Note
It may be necessary to lift the right side of
the cover to clear the bezel indentation
while sliding the cover to the left.

3. Verify that the upper left hand corner of


the cover clears the clip. [3]

Replacement
Action

Install Lower Front Cover

Steps

Reference

1. Install in reverse order of disassembly.

Verification
Action

Steps

Verification of Proper Cover

1. Place instrument into READY mode.

Installation

2. From the MAIN Menu, press RUN followed by SPECIMEN TYPE, then
NORMAL BACKGRND.

3. Press the touch plate to run a cycle.


Note
During the cycle, make sure that cover does not interfere with
instrument operation.

4. Once cycle is complete, verify acceptable background results. Refer to


Specifications & Checklist.

Reference

Removal
Action

Remove
Top Cover

Steps

Reference

1. Using a Phillips-head screwdriver, remove


the two (2) mounting screws [2] on the rear
of the instrument securing the top cover to
the chassis.

2. Lift and remove the top cover [1] by sliding


it to the rear.

Replacement
Action

Install Top Cover

Steps

Reference

1. Install in reverse order of disassembly.

Verification
Action

Steps

Verification of

1. Verify that cutouts on the front

Proper Cover

portion of the top cover [1] are

Installation

placed under the locking nuts [2].

2. Be sure that rear of the cover is


flush against the rear instrument
panel.

Reference

Removal
Action

Steps

Open Upper

1. Open the upper front cover to the left.

Front Cover
Remove
Lower Front

1. Remove Lower Front Cover (RR-A1.02


Lower Front Cover).

Cover
Remove

1. Using a Phillips-head screwdriver, remove

Right Bezel

the left screw [2] on the right side

Screw

cover [3].
Note
The Phillips screw secures the bezel to
the instrument.

Remove

1. Lift the bezel up and away from the

Reference

Bezel

instrument.
Note
Do not pull bezel too far away from
instrument. Several cables must be
removed before completely removing the
bezel from the instrument.

2. Disconnect the following:


o

Ground wire to chassis [7]

Power [3] and data [2] (ribbon)


cable connectors from the rear of
the floppy disk drive [4]

Ribbon cable connector J2 [5] on


the Touch Pad Interconnect
PCB [6].
Note

Mark all cable connectors and note pin 1


(red stripe on ribbon) on the floppy drive
data cable.
3. Remove the screw [8] (Phillips-head)
securing the LCD Interconnect PCB
access cover [1].
4. Disconnect the ribbon cable connected to
JP1A [9] on the LCD Interconnect
PCB [10].

5. Remove the bezel.

Replacement
Action

Install

Steps

1. Install in reverse order of disassembly.

Bezel
Note
During assembly, verify that all cables/connections are secured and are not pinched
between the cover and the chassis.

Verification

Reference

Action

Steps

Reference

Verification of Membrane

1. Power ON instrument, and allow it to initialize.

Keypad Operation

2. During initialization, verify that the LED on the floppy disk drive turns on
and that the LCD display screen is operational.
3. Once instrument is initialized, press RUN to prime system.
4. When in READY mode, press keys on membrane to check for proper
instrument response.

5. If system does not respond, check membrane keypad connection.

Removal
Action

Remove

Steps

Reference

1. Remove all cable connectors

Right Cover

(keyboard [3], COM1 [4], COM2 [5],


LPT1 [6]) from the right side of the
instrument.
2. Using a Phillips-head screwdriver, remove
the left and right screws [2] securing the
right side cover to the instrument frame.

3. Slide the right side cover [1] to the rear of


the instrument about 1/2-inch to clear the
bezel locking nuts, and lift off.

Replacement
Action

Install Right Cover

Steps

Reference

1. Install in reverse order of disassembly.

Verification

Action

Steps

Verification of Proper Cover

Reference

1. Verify that cutouts on front and rear portion of cover are placed

Installation

under locking nuts.

2. Be sure cover is secure against right side of instrument.

Removal
Action

Steps

Remove

Reference

1. Using a Phillips-head screwdriver, remove

Left Cover

the left and right screws [2] securing the


left side cover [1] to the instrument frame.

2. Slide the left cover to the rear of the


instrument about 1/2-inch to clear the
bezel locking nuts and lift off.

Replacement
Action

Steps

Install Left Cover

Reference

1. Install in reverse order of disassembly.

Verification
Action

Steps

Reference

Verification of Proper Cover

1. Verify that cutouts on front portion of cover are placed under

Installation

locking nuts.

2. Be sure cover is secure against left side of instrument.

Flow Panel (CD18-B1)

Removal
Action

Steps

Prerequisite

1. Verify that the instrument is ON.

Open Upper

1. Open the upper front cover.

Front Cover
Remove Lower
Front Cover

Remove RBC
Aperture Plate

1. Remove Lower Front Cover


(RR-A1.02 Lower Front Cover).

1. From the MAIN MENU, press


[SPECIAL PROTOCOLS] then
[MORE] followed by [DRAIN
BATHS]:
2. Locate the von Behrens transducer
and swivel the red Aperture Plate
Retaining Arm [2] outward.

3. Pull the Aperture Plate [1] straight


out.

Remove WBC

1. Repeat the same procedure to

Reference

Aperture Plate

remove the WBC Aperture Plate.


Note
Note the location of the cutout notch
and WBC etched in the Aperture
Plate.

Replacement
Action

Steps

Reference

1. Install in reverse order of disassembly.

Install
Aperture

Note

Plate

The cutout notch [3] in the Aperture Plate


must be facing down and toward the
instrument when the plate is reinserted into
the transducer. Also note the RP etched in
the RBC Aperture Plate and WBC in the
WBC Aperture Plate.

Verification
Action

Verify Background
Counts

Verify Quality
Controls

Steps

1. Run a normal background count cycle and verify results are within acceptable
limits. Refer to Specifications & Checklist.

1. Run three (3) levels of CELL-DYN controls and verify results are within
acceptable assay limits.

Reference

Both the von Behrens RBC and WBC transducers are removed according to the following procedure:

Removal
Action

Prerequisite

Steps

1. If any fluid remains in the


transducers, make sure the
instrument is turned ON.
2. From the MAIN MENU, press
[SPECIAL PROTOCOLS] then
[MORE] followed by [DRAIN
BATHS].

3. Turn the instrument OFF after


the transducers are drained.

Open Upper Front

1. Open the upper front cover.

Cover
Remove Lower Front
Cover

Remove Top Cover

1. Remove Lower Front Cover


(RR-A1.02 Lower Front Cover).

1. Remove Top Cover (RR-A1.03


Top Cover).

Remove Bezel

1. Remove the bezel (RR-A1.04


Bezel).

Remove PAM PCB

1. Remove the Pre-Amplifier


Module (PAM) PCB (RR-F1.01
PAM (Pre-Amplifier Module)).

Prepare to Remove

1. Route cable connector J3

von Behrens WBC

(connected to PAM) through the

Transducer

Flow Panel.

2. Label and disconnect all


tubing [1] from the von Behrens
WBC Transducer [3].

Reference

Remove von Behrens

1. Using a Phillips-head

WBC Transducer

screwdriver, remove the three


(3) mounting screws [2] from the
transducer holding plate.
2. Cut all cable ties securing the
transducer cable to other
cables/chassis.

3. Route transducer cable away


from front panel and remove
transducer.

Prepare to Remove

1. Route cable connector J4

von Behrens

(connecting to PAM) through the

RBC/PLT Transducer

Flow Panel.

2. Label and disconnect all


tubing [3] from the von Behrens
RBC/PLT Transducer [1].

Remove von Behrens

1. Using a Phillips-head

RBC/PLT Transducer

screwdriver, remove the three


(3) mounting screws [2] from the
transducer holding plate.

2. Lift the transducer assembly [1]


out of the instrument.

Replacement
Action

Steps

Install von Behrens Transducer

Reference

1. Install in reverse order of disassembly.

Verification
Action

Verify Gain Settings

Steps

1. Perform VP-09 Signal Processor Module (SPM) Verification/Adjustment.

Reference

Verify Background
Counts

Verify Quality
Controls

1. Run a normal background count cycle and verify results are within acceptable
limits. Refer to Specifications & Checklist.

1. Run three (3) levels of CELL-DYN controls and verify results are within
acceptable assay limits.

WBC Metering PCB Removal

Removal
Action

Prerequisite

Steps

1. Instrument must be in Initialized or


Ready mode.
2. From the MAIN menu, press [SPECIAL
PROTOCOLS] then [MORE] followed
by [DRAIN BATHS]:

3. When the drain baths procedure is


complete, turn OFF the instrument.

Open Upper

1. Open the upper front cover.

Front Cover
Remove Lower
Front Cover

1. Remove Lower Front Cover (RR-A1.02


Lower Front Cover).

Remove Bezel

1. Remove the bezel (RR-A1.04 Bezel).

Remove

1. Using a Phillips-head screwdriver,

Plastic Shield

remove the two (2) screws on the


metering PCB transparent plastic
shield.

Disconnect
Metering Tube
Tubing

1. Disconnect the tubing [4] at the top and


bottom of the metering tube [3].

Reference

Caution
When removing tubing, take extreme
care not to break the glass ends. The
metering tube ends are fragile.

2. Use absorbent tissue to remove any


fluid in the metering tube.
Caution
Take extreme care not to allow the
liquid to touch the printed circuit
boards; it can short them out when
power is turned on. If the board does
get wet, make sure it is dry before
turning power back ON.
Remove WBC

1. Using a 1/4 inch nut driver, remove the

Metering PCB

two (2) standoffs [7] on the board.


2. Remove the ground wire [5] on the
bottom standoff.
3. Disconnect cable connector J1 [6] on
the metering PCB.
4. Using a Phillips-head screwdriver,
remove the two (2) mounting screws [2]
securing the metering PCB to the Flow
Panel.

5. Lift the metering PCB [1] off of the Flow


Panel.

RBC/PLT Metering PCB Removal

Removal
Action

Prerequisite

Steps

1. Instrument must be in Initialized or


Ready mode.
2. From the MAIN menu, press [SPECIAL
PROTOCOLS] then [MORE] followed
by [DRAIN BATHS]:

3. When the drain baths procedure is

Reference

complete, place the instrument in


Standby and turn OFF the power.

Open Upper

1. Open the upper front cover.

Front Cover
Remove Lower
Front Cover

1. Remove Lower Front Cover (RR-A1.02


Lower Front Cover).

Remove Bezel

1. Remove the bezel (RR-A1.04 Bezel).

Remove

1. Using a Phillips-head screwdriver,

Plastic Shield

remove the two (2) screws on the


metering PCB transparent plastic
shield.

Disconnect
Metering Tube

1. Disconnect the tubing [4] at the top and


bottom of the metering tube [3].

Tubing
Caution
When removing tubing, take extreme
care not to break the glass ends. The
metering tube ends are fragile.

2. Use absorbent tissue to remove any


fluid in the metering tube.
Caution
Take extreme care not to allow the
liquid to touch the printed circuit
boards; it can short them out when
power is turned on. If the board does
get wet, make sure it is dry before
turning power back ON.
Remove
RBC/PLT
Metering PCB

1. Using a 1/4 inch nut driver, remove the


two (2) standoffs [7] on the board.
2. Remove the ground wire [5] on the
bottom standoff.

3. Disconnect the cable connector J1 [2]


on the side of the metering PCB.
Note
The RBC Metering PCB [1] is installed

upside down. Push the metering tube


all the way down to access the top
screw securing the metering board to
the flow panel.
4. Using a Phillips-head screwdriver,
remove the two (2) mounting screws [6]
securing the metering PCB to the Flow
Panel.

5. Lift the metering PCB off of the flow


panel.

Replacement
Action

Install Metering PCB

Steps

Reference

1. Install in reverse order of disassembly.


Note
Verify that tubing is not pinched when installing the plastic shield.

Verification
Action

Verify Count
Times

Steps

Reference

1. Verify appropriate count times (see VP-04 Metering System Timing Adjustments RBC and WBC:
o

Verify RBC/PLT count times after installing RBC/PLT metering PCB.

Verify WBC count times after installing WBC metering PCB.

Removal
Action

Prerequisite

Steps

1. From the MAIN MENU, press


[SPECIAL PROTOCOLS] then

Reference

[MORE] followed by [DRAIN BATHS]:

2. Turn instrument power OFF.


Open Upper

1. Open the upper front cover.

Front Cover
Remove Lower
Front Cover

1. Remove Lower Front Cover


(RR-A1.02 Lower Front Cover).

Remove Bezel

1. Remove the bezel (RR-A1.04 Bezel).

Remove Top

1. Remove Top Cover (RR-A1.03 Top

Cover

Open Top Inner


Cover

Cover).

1. Using a Phillips-head screwdriver,


loosen the two (2) screws [1] securing
the top inner cover [2].

2. Lift the top inner cover upward and to


the right.
Note
This cover is hinged and stands in a
folded back position without support.

Remove Top

1. Using a Phillips-head screwdriver,

Shield from

loosen the two (2) screws [4] securing

PAM PCB

the top shield [1] on the PAM PCB [2].

2. Remove the shield.


Route HGB
Cable

1. Disconnect the cable connector from


J2 [3] and route the HGB [7] cable
through the hole [5] at the right side of
the WBC metering PCB [6] and pull it
through the hole.

Remove Tubing Caution


The tubing is stiff. Use care when
removing the tubing to avoid breaking
the HGB Flow Cell.

1. Remove the black tubing from the


T-fitting [2] above and below the HGB
Flow Cell [1].
Note
Use absorbent tissue to capture any
excess liquid in the HGB Flow Cell.
Remove

1. Using a Phillips-head screwdriver,

Hemoglobin

remove the two (2) screws [3]

Flow Cell

securing the HGB flow cell to the flow


panel.

2. Route the HGB cable from behind the


tubing installed at solenoid 2-6 and

remove the HGB Flow Cell module.

Replacement
Action

Steps

Install Hemoglobin Flow Cell

Reference

1. Install in reverse order of disassembly.

Verification
Action

Steps

Verify HGB Offset, Gain,

1. Perform HGB Self Test and Adjust HGB Gain (see Pre-Amp Alignments).

Precision

2. Verify HGB precision.

Verify Background

1. Run a normal background count cycle and verify results are within

Counts

Reference

acceptable limits. Refer to Specifications & Checklist.

Verify Quality Controls

1. Run three (3) levels of CELL-DYN controls and verify results are within
acceptable assay limits.

Fluid Power Supply (CD18-C1)

Removal
Action

Prerequisite

Steps

1. Place the instrument in Standby and


turn OFF the power.

Remove Top
Cover

1. Remove Top Cover (RR-A1.03 Top

Reference

Cover).

Open Top

1. Open Top Inner Cover (RR-B1.04

Inner Cover

Remove
Tubing

Hemoglobin Flow Cell).

Note
Cut any cable ties on cables
surrounding the fluid power supply
assembly as needed.
1. Label and disconnect the tubing from
the T-connector at the top of
solenoid 5-6 [4].
2. Label and disconnect the tubing from
solenoid 5-5 [3].
3. Label and disconnect the silicone
tubing [1] from the top of the 0.5 psi
accumulator located at the rear of the
fluid power supply.
4. Label and disconnect the tubing from
solenoid 5-1 [2].
5. Disconnect the tubing [5] from the
chassis floor fitting [6] next to the left
pump assembly.

6. Disconnect the tubing [7] connected to


the reagent panel waste line (at the rear
of the Reagent Inlet Panel).

Remove

1. Locate solenoid 1-6 and its

Tubing

corresponding silicone tubing [1].

(continued)

Follow the tubing to the back of the


Flow Panel, then label and disconnect
the tubing at the in-line connector.

2. Remove the clamp [2] and plug [3] from


the Vacuum Accumulator drain line [4],
and pull the tubing through the rear of
the Reagent Inlet Panel [5].

Disconnect
Connectors

1. Disconnect cable connector J6 [3] from


the Pump Relay PCB [1].
2. Disconnect cable connector J1 [2] from
the Pump Relay PCB [1].
3. Disconnect cable connector J1
(three-pin cable) from the internal waste
sensor PCB located directly under the
Pump Relay PCB.
4. Disconnect cable connector J9 [3] from
the Solenoid Driver board.
5. Disconnect cable connector J10 [4]
from the Solenoid Driver board.

6. Disconnect cable connector J8 [2]


(bottom connector) from the Solenoid
Driver Module (SDM).

Remove Fluid
Power Supply

1. Using a Phillips-head screwdriver,


remove the three (3) corner mounting
screws and the one (1) central
mounting screw securing the Fluid
Power Supply to the base of the
chassis.

2. Lift the Fluid Power Supply directly up


and out of the instrument.
Note
Verify that all cable connectors and
tubing are disconnected.

Replacement
Action

Install Fluid Power Supply

Steps

Reference

1. Install in reverse order of disassembly.

Verification
Action

Steps

Verify Vacuum, Pressure,

1. Perform VP-03 Vacuum and Pressure Adjustments.

Count Times

2. Verify all count times (see VP-04 Metering System Timing

Reference

Adjustments - RBC and WBC).

Syringe Assembly (CD18-E1)

Removal
Action

Prerequisite

Steps

1. Place the instrument in Standby and


turn OFF the power.

Open Upper

1. Open the upper front cover.

Front Cover
Remove Lower
Front Cover

Remove Top
Cover

Open Top
Inner Cover

Remove
Connectors

1. Remove Lower Front Cover (RR-A1.02


Lower Front Cover).

1. Remove Top Cover (RR-A1.03 Top


Cover).

1. Open Top Inner Cover (RR-B1.04


Hemoglobin Flow Cell).

1. Mark and remove the cable connectors


at the top of the cable distribution
board [1]. There are four cable
connectors that must be unplugged
from this board: J20, J21, J22, and
J23.

2. Cut all cable ties securing cables to


other cables (there are approximately
6-8 cable ties).

Reference

Remove Wash
Block Tubing

1. Using a 3/32-inch hex wrench, remove


the clamp securing the aspiration
tubing [3].
2. Using an adjustable wrench, loosen the
screw securing the two (2) clamps [2]
securing the wash block tubing.
3. Label and disconnect the two (2) tubes
from the Wash Block (top is diluent and
bottom is waste).

4. Route the clamps with attached tubing


through the cutout [4] on the left side of
the Sample Probe Driver assembly
chassis [2].

Remove
Sample Probe

1. Disconnect the tubing from the top of


the Sample Probe.

Tubing
Remove

1. Disconnect the cable connectors

Sample Probe

J1 [2] [5] (ribbon) and J2 [3] [6] (power)

Motor Cable

from both the top [1] and bottom [4]


chopper PCBs.

Remove

1. Using a Phillips-head screwdriver,

Sample Probe

remove the two (2) top and two (2)

Assembly

bottom mounting screws [1] securing


the Sample Probe Driver Assembly to
the chassis.

2. Lift the Sample Probe Driver Assembly


out of the instrument.
Note
Before lifting the Sample Probe Driver
Assembly completely out of the
instrument, check to make sure that all
cables are disconnected. Disconnect
any cables that you find still attached,
making sure to mark them before
unplugging.

Replacement
Action

Install Sample Probe Assembly

Steps

Reference

1. Install in reverse order of disassembly.

Verification
Action

Verify Normal Operation

Steps

1. Run three (3) cycles and verify normal operation, e.g. binding, liquid

Reference

leaks, etc.

1. Perform VP-12 Sample Probe Alignment Check.

Verify Sample Probe


Alignment

Hazard

Removal
Action

Prerequisite

Steps

1. Place the instrument in Standby and


turn OFF the power.

Remove Top

1. Remove Top Cover (RR-A1.03 Top

Cover

Open Top Inner

Cover).

1. Open Top Inner Cover (RR-B1.04

Cover

Remove Left Side

Hemoglobin Flow Cell).

1. Remove the left side cover (see

Cover

Remove Fan

RR-A1.06 Left Cover).

Note
The fan above the Diluent Syringe
Driver Assembly must be removed
before the driver assembly can be
removed.

1. Using a Phillips-head screwdriver,


remove the two (2) top fan
screws [1].
Note
Screws are secured by a nut. Verify
that ALL nuts are removed prior to
removing fan.

2. Using a Phillips-head screwdriver,

Reference

loosen the two (2) bottom fan


bracket screws [2].
Note
If necessary, rotate the split pin
installed on the directional valve
drive linkage of the Diluent Syringe
to allow the fan to be removed.

3. Move the fan to the rear.

Disconnect

1. Disconnect the tubing [3] on both

Diluent Syringe

sides of the directional valve [2] at

Driver Assembly

the top of the Diluent Syringe [1].


Note
Use absorbent tissue to capture any
excess liquid.
2. Disconnect ribbon cable 9520444
from the back of the bottom chopper
PCB.

3. Disconnect ribbon cable 9520445


from the top chopper PCB.

Remove Diluent

Note

Syringe Driver

Lyse Syringe Driver Assembly [1],

Assembly

Diluent Syringe Driver Assembly [2],


and Sample Syringe Driver
Assembly [3].
1. Using a 7/64-inch hex wrench,
remove the two (2) hex screws [5] on
the front of the base plate [4] located
at the bottom of the diluent driver
assembly [2].
2. Using a 7/64-inch hex wrench,
loosen the hex screw [6] from the

rear base plate [4] of the Diluent


Syringe Driver Assembly [2].

3. Slide the Diluent Syringe Driver


Assembly [2] forward, tilt it back, and
remove it through the right side of
the chassis.

Replacement
Action

Steps

Install Diluent Syringe Driver Assembly

Reference

1. Install in reverse order of disassembly.

Verification
Action

Steps

Verify Operation

1. Verify VP-10 Diluent and Sample Verification/Adjustment.

Verify Quality

1. Run three (3) levels of CELL-DYN controls and verify results are within

Reference

Controls

acceptable assay limits.

RR-E1.04 Sample Syringe Driver Assembly

Removal
Action

Prerequisite

Steps

1. Place the instrument in Standby


and turn OFF the power.

Remove Top
Cover

Open Top Inner


Cover

Remove Left Side


Cover

Remove Fan

1. Remove Top Cover (RR-A1.03 Top


Cover).

1. Open Top Inner Cover (RR-B1.04


Hemoglobin Flow Cell).

1. Remove the left side cover (see


RR-A1.06 Left Cover).

1. Remove the fan located above the


Diluent Syringe Driver Assembly
(see Remove Fan, RR-E1.02
Diluent Syringe Driver Assembly).

Disconnect
Tubing

1. Disconnect the tubing [2] on top of


the Sample Syringe Driver
Assembly [1].

Reference

Disconnect

1. Cut the cable ties securing the cable

Connectors

harness to the back of the syringe


assembly.

2. Disconnect ribbon cable 9520441


from the chopper driver PCB.

Remove Sample

Note

Syringe Driver

Lyse Syringe Driver Assembly [1],

Assembly

Diluent Syringe Driver Assembly [2],


and Sample Syringe Driver
Assembly [3].
1. Using a 7/64-inch hex wrench,
remove the two (2) hex screws [5]
on the front base plate [4].
2. Using a 7/64-inch hex wrench,
loosen the hex screw [6] from the
rear base plate [4] of the Sample
Syringe Driver Assembly [3].

3. Slide the Sample Syringe Driver


Assembly [3] forward, tilt it back,
and remove it through the right side
of the chassis.

Replacement
Action

Steps

Reference

1. Install in reverse order of disassembly.

Install Sample Syringe Driver Assembly

Verification
Action

Verify Sample

Steps

Reference

1. Verify sample volume (VP-10 Diluent and Sample Verification/Adjustment).

Volume
Verify Quality
Controls

1. Run three (3) levels of CELL-DYN controls and verify results are within acceptable
assay limits.

RR-E1.06 Lyse Syringe Driver Assembly

Removal
Action

Prerequisite

Steps

1. Place the instrument in Standby

Reference

and turn OFF the power.

Remove Top Cover

1. Remove Top Cover (RR-A1.03


Top Cover).

Open Top Inner

1. Open Top Inner Cover (RR-B1.04

Cover

Remove Left Side

Hemoglobin Flow Cell).

1. Remove the left side cover (see

Cover

Disconnect

RR-A1.06 Left Cover).

1. Disconnect the tubing [2] from the

Tubing/Cables

top of the Lyse Syringe [1].

2. Disconnect ribbon cable 9520446


from the chopper driver PCB.

Remove Lyse

Note

Syringe Driver

Lyse Syringe Driver Assembly [1],

Assembly

Diluent Syringe Driver


Assembly [2], and Sample Syringe
Driver Assembly [3].
1. Using a 7/64-inch hex wrench,
remove the two (2) hex screws [5]
on the front base plate [4].
2. Using a 7/64-inch hex wrench,
loosen the hex screw [6] from the
rear base plate [4] of the Lyse
Syringe Driver Assembly [1].

3. Slide the Lyse Syringe Driver

Assembly forward, tilt it back, and


remove it through the right side of
the chassis.

Replacement
Action

Steps

Reference

1. Install in reverse order of disassembly.

Install Lyse Syringe Driver Assembly

Verification
Action

Verify Quality
Controls

Steps

Reference

1. Run three (3) levels of CELL-DYN controls and verify results are within
acceptable assay limits.

Electronics / Card Cage (CD18-F1)


RR-F1.01 PAM (Pre-Amplifier Module)

Removal
Action

Prerequisite

Steps

1. Place the instrument in Standby and


turn OFF the power.

Reference

Open Upper

1. Open the upper front cover.

Front Cover
Remove Lower

1. Remove Lower Front Cover

Front Cover

(RR-A1.02 Lower Front Cover).

Remove Top

1. Remove Top Cover (RR-A1.03 Top

Cover

Cover).

Remove Bezel

1. Remove the bezel (RR-A1.04 Bezel).

Flip Bezel Latch

1. Using a Phillips-head screwdriver,

Bracket UP

remove the lower screw [1] on the


bezel latch bracket [2] attached to the
chassis.
2. Loosen, but do not remove the top
screw [3] on the latch.

3. Flip the latch up and to the right.


Remove Top

1. Using a Phillips-head screwdriver,

Shield from

loosen the two (2) screws [4] securing

PAM PCB

the top shield [5] on the PAM PCB.

2. Remove the shield.


Remove PAM
Cover

1. Using a Phillips-head screwdriver,


remove the two (2) left retaining
screws [4] [6] on the left side of the
PAM cover plate and loosen the two
(2) right retaining screws [7].

2. Slide the PAM cover [8] to the left and


off the PAM PCB.

Disconnect
Cables

1. Mark and disconnect the five (5) cable


connectors J1 [1], J2 [2], J3 [3],
J4 [4], and J5 [5] from the PAM PCB.
Note
The J3 connector is for WBC and the
J4 connector is for RBC/PLT.

Remove PAM
Board

1. Using a 1/4-inch nut driver, remove


the four (4) metal standoffs [6], on the

corners of the PAM PCB.

2. Lift the PAM PCB off the Flow Panel.

Replacement
Action

Install

Steps

Reference

1. Install in reverse order of disassembly.

PAM
Board

Note
Be sure that the metal standoffs securing the PCB in place and the screws securing the
metal cover over the PCB are securely fastened. Failure to properly secure the standoffs
and cover screws may result in electrical noise being induced due to improper grounding.

Verification
Action

Verify PAM Printed Circuit Board

Steps

1. Perform VP-08 Pre-Amplifier Module (PAM) Adjustment.

Adjustments
Verify SPM Printed Circuit Board
Adjustments

1. Perform VP-09 Signal Processor Module (SPM)


Verification/Adjustment.

RR-F1.02 MPM (Motor Processor Module) Board

Reference

Removal
Action

Prerequisite

Steps

1. Place the instrument in Standby and


turn OFF the power.

Remove Top
Cover

Open Top
Inner Cover

Remove
Cables

Remove MPM
Board

1. Remove Top Cover (RR-A1.03 Top


Cover).

1. Open Top Inner Cover (RR-B1.04


Hemoglobin Flow Cell).

1. Label and disconnect cable connectors


(leading to the MPM PCB):
o

J1 [1]

J2 [2]

J3 [3]

J4 [4]

J5 [5]

J6 [6]

J7 [7]

J10 [8]

1. At each corner of the MPM PCB,


compress the plastic snap-in pins and
pull the corner of the PCB free from the
pin.
Note
Repeat this procedure for the snap-in
pin at the center of the PCB.

2. Lift the MPM PCB off the top inner


cover.

Reference

Replacement
Action

Steps

Reference

1. Install in reverse order of disassembly.

Install MPM Board

Verification
Action

Steps

Verify Motor Operation

Reference

1. Perform VP-11 Stepper Motor Power Test and Verification.

RR-F1.03 CDM (Cable Distribution Module) Board

Removal
Action

Prerequisite

Steps

1. Place the instrument in Standby and


turn OFF the power.

Remove Top
Cover

Open Top
Inner Cover

Disconnect
Cables

1. Remove Top Cover (RR-A1.03 Top


Cover).

1. Open Top Inner Cover (RR-B1.04


Hemoglobin Flow Cell).

1. Label and disconnect the following


cable connectors (leading to the CDM
PCB):
o

J1 [15]

J2 [14]

J3 [13]

J4 [12]

J5 [11]

Reference

Remove CDM
Board

J6 [10]

J7 [9]

J8 [8]

J9 [7]

J11 [16]

J14 [17]

J15 [18]

J16 [19]

J17 [20]

J18 [21]

J19 [22]

J20 [1]

J21 [2]

J22 [3]

J23 [4]

J31 [5]

J32 [23]

1. At each corner of the CDM PCB,


compress the plastic snap-in pin and
pull the corner of the PCB free from the
pin.
Note
Repeat this procedure for the snap-in
pin at the center of the PCB.

2. Lift the CDM PCB off the top inner


cover.

Replacement
Action

Install CDM Board

Steps

Reference

1. Install in reverse order of disassembly.

Verification
Action

Steps

Reference

1. Perform VP-07 Cable Distribution Module Test Points.

Verify CDM PCB


Voltage
Verify Normal Operation

1. Run three (3) cycles and verify normal operation, e.g. binding, liquid leaks,
etc.

RR-F1.04 Hard Disk Drive

Removal
Action

Prerequisite

Steps

1. Place the instrument in Standby


and turn OFF the power.

Open Upper Front

1. Open the upper front cover.

Cover
Remove Lower
Front Cover

Remove Bezel

1. Remove Lower Front Cover


(RR-A1.02 Lower Front Cover).

1. Remove the bezel (RR-A1.04


Bezel).

Remove Right
Side Cover

Remove Front
Card Cage Shield

1. Remove the right side cover


(RR-A1.05 Right Cover).

1. Using a Phillips-head screwdriver,


remove the two (2) screws [1]
securing the Front Card Cage
Shield [2] to the chassis.

2. Remove the shield from the


instrument.

Reference

Prepare to

1. Disconnect the Backplane PCB

Remove Card

Power cable from the DC Regulator

Cage from

PCB.

Chassis

2. Disconnect the Disk Drive Power


Cable from the DC Regulator PCB.
3. Remove the two (2) screws securing
the top section of the backplane
PCB shield and remove the shield.

4. Disconnect cable connectors J9 and


J10 on the Backplane PCB.

Remove Card
Cage

1. Using a Phillips-head screwdriver,


remove the four (4) screws [1]
securing the card cage [2] to the
chassis.
Caution
Once the screws are removed, the
card cage drops down. Excessive
movement on the card cage can
damage connectors on the
Backplane PCB.

Remove Hard
Disk Drive

1. Slide the bottom of the card cage


forward and tilt it backward.
Note

Verify that all four (4) screws


securing the hard disk drive to the
card cage are accessible.
2. Using a Phillips-head screwdriver,
remove the four (4) screws [1]
securing the hard disk drive [2] to
the card cage.

3. Note the alignment of the red


stripe [5] on the ribbon cable in
relationship to the hard disk drive
connector.
Note
The same alignment must be
maintained during replacement.
4. Disconnect the power [3] and data
cable [4] connectors (rear of hard
disk drive).

5. Remove the hard drive from the


instrument.

Replacement
Action

Steps

Reference

Install Hard Disk Drive

1. Install in reverse order of disassembly.

Verification
Action

Steps

Verify Hard Disk Drive Setup

Reference

1. Perform VP-14 Hard Disk Drive Setup and Verification.

RR-F1.05 Floppy Disk Drive

Removal
Action

Prerequisite

Steps

1. Place the instrument in Standby and


turn OFF the power.

Open Upper

1. Open the upper front cover.

Front Cover
Remove Lower
Front Cover

1. Remove Lower Front Cover


(RR-A1.02 Lower Front Cover).

Remove Bezel

1. Remove the bezel (RR-A1.04 Bezel).

Remove Floppy

1. Using a Phillips-head screwdriver,

Disk Drive

loosen the four (4) screws [1] securing


the floppy drive mounting bracket [2]
to the bezel.
2. Using a Phillips-head screwdriver,
remove the four (4) screws [3]
securing the floppy drive to the
mounting bracket [4].

3. Remove the floppy disk drive from the


instrument.

Reference

Replacement
Action

Install Hard Disk Drive

Steps

Reference

1. Install in reverse order of disassembly.

Verification
Action

Steps

Verify Floppy Disk Drive

1. Power ON instrument, and allow it to initialize.

Operation

2. During initialization, verify that the LED on the floppy disk drive turns on and
that the LCD display screen is operational.

Reference

RR-F1.06 Signal Processor Module (SPM)

Removal
Action

Prerequisite

Steps

1. Place the instrument in Standby and


turn OFF the power.

Open Upper

1. Open the upper front cover.

Front Cover
Remove Lower

1. Remove Lower Front Cover

Front Cover

(RR-A1.02 Lower Front Cover).

Remove Bezel

1. Remove the bezel (RR-A1.04 Bezel).

Remove Front

1. Using a Phillips-head screwdriver,

Card Cage

remove the two (2) screws [1]

Shield

securing the Front Card Cage


Shield [2] to the chassis.

2. Remove the shield from the


instrument.

Remove SPM
PCB

Note
Card cage PCBs from left to right:
SPM, CCM, DLA and SBC.
1. Disconnect cable connector J1 [2]
from the SPM PCB [1].

2. Open the top and bottom plastic


mounting tabs.
Note

Reference

Pulls the board slightly out of the card


cage.

3. Pull the board out of the instrument.

Replacement
Action

Install SPM

Steps

Reference

1. Install in reverse order of disassembly.

PCB
Note
When reinserting the SPM PCB into its slot at the rear of the card cage, press it firmly
into place to ensure proper seating.

Verification
Action

Verify SPM PCB

Steps

1. Perform VP-09 Signal Processor Module (SPM) Verification/Adjustment.

Adjustments
Note
Make SPM adjustments in the following order:

Verify Background
Counts

RBC

RER

WBC

PLT

1. Run a normal background count cycle and verify results are within acceptable
limits. Refer to Specifications & Checklist.

Reference

1. Run three (3) levels of CELL-DYN controls and verify results are within

Verify Quality Controls

acceptable assay limits.

RR-F1.07 Cell Count Module (CCM)

Removal
Action

Prerequisite

Steps

1. Place the instrument in Standby and


turn OFF the power.

Open Upper

1. Open the upper front cover.

Front Cover
Remove Lower

1. Remove Lower Front Cover

Front Cover

(RR-A1.02 Lower Front Cover).

Remove Bezel

1. Remove the bezel (RR-A1.04 Bezel).

Remove Front

1. Using a Phillips-head screwdriver,

Card Cage

remove the two (2) screws [1]

Shield

securing the Front Card Cage


Shield [2] to the chassis.

2. Remove the shield from the


instrument.

Remove CCM
PCB

Note
Card cage PCBs from left to right:
SPM, CCM, DLA and SBC.
1. Disconnect cable connectors J1
through J3, and J5 from the CCM

Reference

PCB.

2. Open the top and bottom plastic


mounting tabs [1].
Note
Pulls the board slightly out of the card
cage.

3. Pull the board out of the instrument.

Replacement
Action

Install CCM

Steps

Reference

1. Install in reverse order of disassembly.

PCB
Note
When reinserting the CCM PCB into its slot at the rear of the card cage, press it firmly
into place to ensure proper seating.

Verification
Action

Verify Background
Counts

Verify Quality
Controls

Steps

1. Run a normal background count cycle and verify results are within acceptable
limits. Refer to Specifications & Checklist.

1. Run three (3) levels of CELL-DYN controls and verify results are within
acceptable assay limits.

RR-F1.08 Data Link Adapter (DLA)

Removal

Reference

Action

Prerequisite

Steps

1. Place the instrument in Standby and


turn OFF the power.

Open Upper

1. Open the upper front cover.

Front Cover
Remove Lower

1. Remove Lower Front Cover

Front Cover

(RR-A1.02 Lower Front Cover).

Remove Bezel

1. Remove the bezel (RR-A1.04 Bezel).

Remove Front

1. Using a Phillips-head screwdriver,

Card Cage

remove the two (2) screws [1]

Shield

securing the Front Card Cage


Shield [2] to the chassis.

2. Remove the shield from the


instrument.

Remove DLA
PCB

Note
Card cage PCBs from left to right:
SPM, CCM, DLA and SBC.
1. Disconnect cable connectors J1 [2]
and J2 [3] from the DLA PCB [1].
2. Using a Phillips-head screwdriver,
remove the screw securing the DLA
PCB bracket to the top of the card
cage.

3. Grasp the top and bottom of the DLA


PCB and pull the card from the card
cage and away from the instrument.

Reference

Replacement
Action

Install DCM

Steps

Reference

1. Install in reverse order of disassembly.

PCB
Note
When reinserting the DLA PCB into its slot at the rear of the card cage, press it firmly
into place to ensure proper seating.

Verification
Action

Verify Background
Counts

Steps

Reference

1. Run a normal background count cycle and verify results are within acceptable
limits. Refer to Specifications & Checklist.

RR-F1.09 Single Board Computer (SBC)

Removal
Action

Prerequisite

Steps

1. Place the instrument in Standby and


turn OFF the power.

Open Upper

1. Open the upper front cover.

Front Cover
Remove Lower
Front Cover

1. Remove Lower Front Cover


(RR-A1.02 Lower Front Cover).

Remove Bezel

1. Remove the bezel (RR-A1.04 Bezel).

Remove Front

1. Using a Phillips-head screwdriver,

Card Cage

remove the two (2) screws [1]

Shield

securing the Front Card Cage


Shield [2] to the chassis.

2. Remove the shield from the

Reference

instrument.

Remove SBC

Note
Card cage PCBs from left to right:

PCB

SPM, CCM, DLA and SBC.


1. Label and disconnect all cable
connectors from the SBC PCB [1].
2. Using a Phillips-head screwdriver,
remove the screw securing the SBC
PCB bracket to the top of the card
cage.

3. Grasp the top and bottom of the SBC


PCB and pull the card from the card
cage and away from the instrument.

Replacement
Action

Install SBC

Steps

Reference

1. Install in reverse order of disassembly.

PCB
Note
When reinserting the SBC PCB into its slot at the rear of the card cage, press it firmly
into place to ensure proper seating.

Verification
Action

Steps

Reference

Verify Background
Counts

1. Run a normal background count cycle and verify results are within acceptable
limits. Refer to Specifications & Checklist.

RR-F1.10 Card Cage Backplane PCB

Removal
Action

Prerequisite

Steps

1. Place the instrument in Standby and


turn OFF the power.

Open Upper

1. Open the upper front cover.

Front Cover
Remove Lower
Front Cover

1. Remove Lower Front Cover


(RR-A1.02 Lower Front Cover).

Remove Bezel

1. Remove the bezel (RR-A1.04 Bezel).

Remove Front

1. Using a Phillips-head screwdriver,

Card Cage

remove the two (2) screws [1]

Shield

securing the Front Card Cage


Shield [2] to the chassis.

2. Remove the shield from the


instrument.

Remove SPM

1. Remove SPM PCB.

PCB
Remove CCM
PCB

1. Remove CCM PCB.

Reference

Remove DLA

1. Remove DLA PCB.

PCB
Remove SBC

1. Remove SBC PCB.

PCB
Remove

1. Using a Phillips-head screwdriver

Backplane PCB

(stubby), remove the top three (3)

Shield

screws [2] securing the Backplane


PCB Shield [1] to the card cage [3].
2. Using a Phillips-head screwdriver
(stubby), remove the bottom three (3)
screws [4] securing the Backplane
PCB Shield [2] to the card cage [3].
3. Disconnect cable connectors J1, J9,
and J10 from the Backplane PCB.

4. Remove the shield from the


instrument.

Remove
Backplane PCB

1. Verify that all screws and cable


connections are removed from the
Backplane PCB.

2. Remove the Backplane PCB from the


instrument.

Replacement
Action

Steps

Reference

Install Backplane

1. Install in reverse order of disassembly.

PCB
Note
When reinserting the backplane PCB into its slot at the rear of the card cage, press
it firmly into place to ensure proper seating.

Verification
Action

Steps

Verify Normal Operation

Reference

1. Place instrument in Ready mode.

2. Verify that instrument properly initializes and primes.


Verify Card Cage Backplane

1. Perform VP-06 Card Cage Backplane Test Points.

PCB Voltages
Verify Background Counts

1. Run a normal background count cycle and verify results are within
acceptable limits. Refer to Specifications & Checklist.

Verify Quality Controls

1. Run three (3) levels of CELL-DYN Controls and verify results are within
acceptable assay limits.

LCD/Keyboard (CD18-G1)

Removal
Action

Prerequisite

Steps

1. Place the instrument in Standby and


turn OFF the power.

2. Unplug power cord from rear of


instrument.

Open Upper

1. Open the upper front cover.

Front Cover
Remove Lower
Front Cover

1. Remove Lower Front Cover

Reference

(RR-A1.02 Lower Front Cover).

Remove Bezel

1. Remove the bezel (RR-A1.04


Bezel).

Remove LCD
Display Screen
Assembly

1. Place bezel cover face down on a


flat surface.
2. Disconnect cable connector CN2 [3]
from the backlight inverter PCB [4].
3. Release the backlight wires from the
cable clip.
4. Using a Phillips-head screwdriver,
remove the four (4) screws [5]
securing the LCD Display Screen
mounting plate to the bezel cover.
5. Using a Phillips-head screwdriver,
remove the four (4) screws [1]
securing the LCD Display Screen [6]
to the mounting plate.

6. Disconnect the LVDS cable


connector [7] at the rear of the LCD
Display Screen.
Note
When disconnecting the LVDS cable
connector, pull the connector, NOT
the wires.

Replacement
Action

Install the LCD Display

Steps

1. Install in reverse order of disassembly.

Screen Assembly
Note
During replacement, verify that the LCD Display Screen assembly is
centered in the bezel cover, before securing the assembly to the cover.

Reference

Verification
Action

Verify LCD
Display Screen
Installation

Steps

Reference

1. Turn the instrument Power ON and


allow the instrument to initialize.
2. Verify that the LCD Display Screen
is turned on and centered in the
bezel.

3. Verify that the LVDS cable (between


the LCD display [3] and the LCD
Interconnect PCB [1]) is routed
correctly [1-3]
Note
Failure to properly route and secure
the LVDS cable may result in
damage to the wires and connector.

Power Supply (CD18-H1)

Removal
Action

Prerequisite

Steps

1. Place the instrument in Standby


and turn OFF the power.
Note
Never touch a PCB when the power
is ON.

2. Unplug power cord from rear of


instrument.

Open Upper Front

1. Open the upper front cover.

Cover
Remove Lower

1. Remove Lower Front Cover

Reference

Front Cover

(RR-A1.02 Lower Front Cover).

Remove Top

1. Remove Top Cover (RR-A1.03 Top

Cover

Remove Bezel

Cover).

1. Remove the bezel (RR-A1.04


Bezel).

Remove Right

1. Remove the right side cover

Side Cover

Remove Cable

(RR-A1.05 Right Cover).

1. Label and disconnect the four (4)

Connectors from

cable connectors at the rear of the

Power Switch

power switch [1].


Note
The power switch contains two (2)
black and two (2) white cable
connectors.

Remove Cable

1. Label and disconnect cable

Connectors from

connectors J3 through J9, and J11

DC Regulator PCB

from the DC Regulator PCB [2].

Remove Cable

Note

Connectors from

Cut cable ties from cable bundles

AC Regulator PCB

containing cable connector J7.


1. Label and disconnect the cable
connectors J5, J7 and J9 from the
AC Regulator PCB [1].
2. Label and disconnect the chain
cable connector J6 from the AC
Regulator PCB and J2 from the DC
Regulator PCB.

3. Release the chain cable connector

from the cable clamp attached to


the switching power supply.

Remove Power
Supply Assembly

1. Using a Phillips-head screwdriver,


remove the four (4) screws [1] from
the rear of the instrument securing
the power supply assembly to the
instrument chassis.
2. Carefully lift the power supply up,
then partially out of the instrument.

3. Verify that no cable connectors are


connected to the power supply
assembly.
Caution
The power switch is vulnerable to
damage during this process.

4. Remove the power supply


assembly.

Replacement
Action

Steps

Install Power Supply Assembly

Reference

1. Install in reverse order of disassembly.

Verification
Action

Verify System Voltages

Steps

1. Perform VP-06 Card Cage Backplane Test Points.

Reference

Verification Procedures
VP-01 Preparation for Alignment and Verification
VP-01 Preparation for Alignment and Verification

Purpose

Preparation for Alignments/Verifications.

Module CELL-DYN 1800

Materials Required See Test Equipment and Supplies Required. Time

Test Equipment and Supplies Required

ITEM

DESCRIPTION

QTY

Digital Volt Meter

5-inch Jumper Leads

Stopwatch

Vacuum Gauge 0-30 Inches

Pressure Gauge 0-5 lb.

Pressure Gauge 0-10 lbs.

Polystyrene Microspheres, 5.0 or 5.01 m

Polystyrene Microspheres, 3.31 m

10

Hemostats

11

500 mL Flask or Beaker

12

Silicone Tubing

AR

01:00 hr

13

Standard Tool Kit

14

Fresh Blood Samples With Reference Values

AR

15

Assayed Controls for CELL-DYN 1800

AR

16

20k Ohm 1% Resistor

17

15k Ohm 1% Resistor

18

10 mL Graduated Cylinder

19

40 L Micropipettes

AR

20

100 L Micropipettes

AR

21

50 mL Counting Cups

AR

22

25 mL Graduated Cylinder

23

12-inch Ruler With 1/16 Increments

24

Oscilloscope (Optional)

AR = As Required

Preparation for Alignment/Verification


Perform the following procedure to prepare the CELL-DYN 1800 System for alignment/verification:

Action

Steps

Check Reagents

1. Verify all reagents are correct and are 1/3 full.

Remove Upper

1. Remove Upper Front Cover (RR-A1.01 Upper Front Cover).

Front Cover
Remove Lower

1. Remove Lower Front Cover (RR-A1.02 Lower Front Cover).

Front Cover
Remove Left

1. Remove Left Cover (RR-A1.06 Left Cover).

Cover
Remove Right

1. Remove Right Cover (RR-A1.05 Right Cover).

Cover
Remove Top

1. Remove Top Cover (RR-A1.03 Top Cover).

Reference

Cover
Clean Aperture
Plates

1. Remove and clean both RBC/PLT and WBC aperture plates following the
procedure in Section 9: Service and Maintenance, Subsection:
Cleaning/Replacing Aperture Plates of the CELL-DYN 1800 Operator's Manual.

Clean HGB Flow


Cell

Re-initialize

1. Clean the HGB Flow Cell following the procedure in Section 9, Subsection:
Cleaning the HGB Flow Cell of the CELL-DYN 1800 Operator's Manual.

1. Re-initialize the instrument by turning the system OFF then ON again.

Instrument
Check Tubing,

1. When Initialization is complete, press [PRIME/RUN] to prime the instrument.

Look For Leaks

Observe the flow system for leaks, tubing placement, pinched tubing, etc.

Install Covers

1. Reinstall the covers on the instrument.

Run Background

1. Run a Background count. Verify all background values are within the following

Count

specifications:
WBC

0.5 K/L

RBC

0.05 M/L

HGB

0.1 g/dL

PLT

10.0 K/L

Record

1. In the MAIN MENU, press [CALIBRATION].

Calibration

2. Record all Calibration Factors for Open and Pre-Dilute modes.

Factors
Record Dilution
Factors

Enter Service
Personnel ID

1. Type 94043 to display the DILUTION FACTORS screen. Record all Dilution
Factors.

1. In the MAIN MENU, type 999 for Operator ID and press Enter on the PC keyboard
or membrane keypad. This is to identify all runs performed by service personnel.

Order of Alignment/Verification
The following procedures are presented to ensure proper alignment of the CELL-DYN 1800 System.

Sequence

Procedure

VP-03 Vacuum and Pressure Adjustments

VP-04 Metering System Timing Adjustments - RBC and WBC

VP-06 Card Cage Backplane Test Points

VP-07 Cable Distribution Module Test Points

VP-08 Pre-Amplifier Module (PAM) Adjustment

VP-09 Signal Processor Module (SPM) Verification/Adjustment

VP-10 Diluent and Sample Verification/Adjustment

VP-11 Stepper Motor Power Test and Verification

VP-12 Sample Probe Alignment Check

When performing adjustments:

At the completion of each Vacuum and Pressure Adjustment procedure, verify the WBC and RBC count times and
normal system operation

Prior to starting any electronic alignment, verify the power supply voltages in VP-06 Card Cage Backplane Test
Points and VP-07 Cable Distribution Module Test Points.

Whenever an alignment adjustment is performed on the Pre-Amplifier Module (PAM), verify all parameters on the
Signal Processor Module (SPM).

VP-02 Decontamination

Purpose

To decontaminate all surfaces before servicing. Module CELL-DYN 1800

Materials Required Sodium hypochorite solution

Action

Decontamination
Ratio

Time

00:15 min

Steps

1. The OSHA Bloodborne Pathogen Rule (29 CFR Part 1910.1030) requires the
decontamination of laboratory equipment prior to servicing or shipment:
o

Decontaminate the instrument by performing the Auto-Clean cycle. This


cycle flushes all of the fluid pathways with reagents to purge any waste from
the fluid pathways. The Open Mode Sample Probe is automatically rinsed

Reference

after every cycle. The surfaces of the instrument should be wiped with a
nonabrasive detergent solution to remove any soiling, then wiped with a
tuberculocidal disinfectant, such as a 0.5% sodium hypochlorite solution.

2. To calculate the percent (%) sodium hypochlorite concentration desired, use the
following formula:
a. A = Percent (%) of sodium hypochlorite solution desired
b. B = Percent (%) of sodium hypochlorite stock solution (as purchased)
c. X = Parts of water to be mixed with one part of the sodium hypochlorite stock
solution:

d. Example: If you need a 0.5% solution of sodium hypochlorite for a cleaning


procedure, and the label on the bottle of bleach states that it is 5.25%
sodium hypochlorite, then:

e. Add 9.5 parts deionized water to 1 part bleach to obtain a 0.5% sodium
hypochlorite solution, or 9.5 mL of deionized water to 0.5 mL of bleach
(5.25% sodium hypochlorite) to obtain 10 mL of a 0.5% solution of sodium
hypochlorite.

3. If the instrument is to be shipped, it must be decontaminated prior to shipment. This


is accomplished by pressing the [CLEAN FOR SHIPPING] key in the SPECIAL
PROTOCOLS menu. Instructions for this procedure are given in the
CELL-DYN 1800 Operator's Manual, (Section 9 Preparing the Instrument for
Extended Periods of Non-Use or Shipping).

VP-03 Vacuum and Pressure Adjustments

Purpose

Align and adjust pressure and vacuum. Module CELL-DYN 1800

Materials Required Digital Volt Meter (DVM)


0-5 psi Pressure Gauge
0-30 psi Pressure Gauge
0-30 HG Vacuum Gauge
Phillips Screwdriver
Thin Flat Blade Screwdriver

Time

00:30 min

This procedure consist of four parts (perform in sequence):

Regulator Alignment
Pressure Adjustment (0.5 psi)
Pressure Verification (High)
Vacuum Adjustment (8 inch)

The CELL-DYN 1800 System utilizes one vacuum and two pressure levels to accomplish the following tasks: moving sample,
reagents, and waste, bubble mixing of sample, and back flushing RBC and WBC apertures. The vacuum and bubble mix
pressures are adjustable by a solid-state regulator. The backflush pressure is not critical and the pump is under direct
computer control.
The solid state regulator has two input ports: P1 for pressure and P2 for vacuum. It also has jumper terminals which
accommodate all desired vacuum and pressure ranges. The jumper positions are:

Jumpers Pressure (psi) Vacuum (inches Hg)

A-B

0.0 - 3.0

0.0 - 6.0

C-D

2.5 - 5.1

5.0 - 10.2

E-F

4.3 - 6.6

8.6 - 13.2

G-H

6.0 - 8.0

12.0 - 16.0

Regulator Alignment

Action

Access Pump
Relay Board

Steps

Note
This offset adjustment must be performed with no pressure or vacuum applied to
the regulator.

1. Disconnect the appropriate pumps on the Pump Relay Board and bleed off

Reference

pressure from the Accumulators.


o

J4 for low pressure

J2 for vacuum on new-style pumps

2. Remove the pressure or vacuum line from the top of the regulator.
Measure Voltage

1. On the Pump Relay Board the voltage should be 5.0 0.15 volts at J7 pin 7 for
vacuum, and J8 pin 5 for pressure.
Note
You must remove the board mounting screws and free the rest of the board to
make the remaining adjustments. Disconnect J6 or J7 while relocating the
appropriate printed circuit board to avoid shorting out components.
2. Note the current position of jumper E1 and set E1 to the C-D position.

3. Connect the DVM (Digitial Volt Meter) negative lead to TP3 (GND) on regulator
board. Connect the positive lead to TP2 (REF). The voltage should be
1.00V 0.14 volts.
o

If voltage is not correct, re-check Step 1.

If voltage is still not correct, replace the regulator.

4. Connect the DVM negative lead to TP1 and the positive lead to TP2, and read the
voltage. Adjust R18 for a voltage of 0.000 0.005.
Note
If voltage is negative, turn R18 (offset) clockwise; if the voltage is positive, turn R18
counterclockwise.
Reposition E1

1. Move jumper E1 back to the proper operating position.

Reconnect

1. Reconnect the pressure or vacuum line to the top of the regulator and reconnect

Pressure / Vacuum

the cable(s) on the pump relay module.

Line

Pressure Adjustment (0.5 psi)


Follow the steps below for pressure adjustment:

Action

Remove Top
Cover

Steps

1. Remove Top Cover (RR-A1.03 Top Cover).

Reference

Open Top Inner

1. Open Top Inner Cover (RR-B1.04 Hemoglobin Flow Cell).

Cover
Connect
Pressure Gauge

1. Locate the small silicone tubing connected to the in-line fitting at top of the 0.5 psi
Pressure Accumulator (located closest to the rear of the instrument).

2. Connect a 0-5 psi gauge in-line with the silicone tubing and fitting (the Pressure
Accumulator is located on the part of the Fluid Power Supply which is closest to the
rear of the instrument).

Adjust

1. Adjust R16 on the Regulator board for 0.55 psi 0.02.

Regulator
Note
Clockwise adjustment increases pressure and counterclockwise adjustment
decreases pressure.

2. Remove the gauge and reconnect the silicone tubing to in-line connector.

Pressure Verification (High)


Follow the steps below for pressure verification:

Action

Locate In-line
Connector

Steps

1. Locate solenoid valve 1-6 and trace the tubing back through the flow panel to the
in-line connector.

Connect Pressure

1. Connect a 0-30 psi gauge in-line.

Gauge

2. In the RUN screen, press [CLEAR ORIFICE] while observing the gauge.

Verify Correct

1. When the pump activates, verify a pressure of no less than 4 psi.

Pressure

If the pressure is less than 4 psi, check for leaks. Replace the Pressure
Pump if necessary. (Refer to RR-C1.01 Fluid Power Supply.)

Vacuum Adjustment (8 inch)


Follow the steps below for solid state regulator adjustment:

Reference

Action

Locate Vacuum

Steps

Reference

Note
This is a coarse adjustment only. The fine adjustment is predicated upon metering

Regulator

system count times. Make this coarse adjustment only if there is reason to believe that
the vacuum is grossly misadjusted.

1. Locate the solid state vacuum regulator on the rear side of the Fluid Power Supply
(closest to the flow panel).

Adjust R16

1. Remove the TYGON tubing from the top of the Vacuum Accumulator (located on the
back of the Fluid Power Supply closest to the flow panel) and connect a 0-30" Hg
gauge in-line between the tubing and the Accumulator.

2. Adjust R16 (accessible only from the front of the Fluid Power Supply) for
8.25" Hg 0.25".
Note
Clockwise adjustment increases vacuum and counterclockwise adjustment decreases
vacuum.
Reconnect

1. Once R16 is adjusted, remove the vacuum gauge and reconnect the tubing line to the

Tubing Line

regulator.

VP-04 Metering System Timing Adjustments - RBC and WBC

Purpose

Adjust Metering System to regulate count times. Module CELL-DYN 1800

Materials Required Phillips Screwdriver

This procedure consist of two parts:

Links

RBC Metering System Timing Adjustment


WBC Metering System Timing Adjustment

Time

00:15 min

The instrument uses the Volumetric Metering process to regulate the count cycle and to ensure that a precise volume of
sample is analyzed for the measurement. This table lists the results of a fault report displayed on the screen if a flow error or
clog occurs during a run cycle.
Metering Timing Fault Report

Detector Masked Flow Error Time Valid Meniscus Time


(in seconds)

(in seconds)

Clog Time

(in seconds)

(in seconds)

WBC Upper

0 - 0.5

0.5 - 1.0

1.0 - 3.0

3.0+

WBC Lower

0 - 3.5

3.5 - 4.0

4.0 - 6.5

6.5+ or greater than the moving average

RBC/PLT Upper 0 - 3.0

3.0 - 4.0

4.0 - 6.0

6.0+

RBC/PLT Lower 0 - 5.5

5.5 - 6.0

6.0 - 7.5

7.5+ or greater than the moving average

This figure illustrates the timing relationships for WBC and RBC measurements.
CELL-DYN 1800 Metering Timing Chart (in Seconds)

For each transducer there are two distinct counting periods, T1 and T2. RBC Metering Tube and WBC Metering Tube
illustrate the counting periods for the RBC and WBC metering tubes, respectively.
RBC Metering Tube

WBC Metering Tube

All count times are based on the vacuum level of the 8-inch vacuum reservoir and tubing length. These count times are
critical in that all clog and flow system alarms are generated via these count times. The vacuum adjustment (Vacuum
Adjustment (8 inch)) sets the coarse vacuum requirement, but further adjustments are necessary to ensure proper timing for
the flow system alerts.
RBC Metering System Timing Adjustment
Follow the steps below for adjusting the timing of the RBC metering system:

Action

Verify

Steps

1. Ensure that the RBC aperture plate has been removed, cleaned, and reinstalled following

Count

the procedure in Section 9: Service and Maintenance, Subsection: Cleaning/Replacing

Time

Aperture Plates of the CELL-DYN 1800 Operator's Manual.

2. Run a background count and verify that the RBC displayed count time (T2) is 6.7 seconds
0.2 seconds.
o

If the specification is met, go to Step 4.

If the specification is not met, go to Step 3.

3. Readjust the vacuum to correct the count time if the time is out of specification (refer to
Adjust R16, Step 2 of the Vacuum Adjustment (8-inch) procedure previously discussed).
4. From the MAIN Menu, press [DIAGNOSTICS] followed by [RAW DATA].

5. Verify the time displayed for RBC UPTIME is 4.8 to 5.2 seconds. (This time, displayed in
milliseconds, represents T1, which is the time when the RBC valve 1-2 opens until the
meniscus reaches the upper detector.)

Reference

If the specification is met, the procedure is complete.

If the specification is not met, go to Step 6.

6. Adjust the metering tube.


o

Raise the metering tube to increase the upper time.

Lower the metering tube to decrease the upper time.

7. Repeat Step 2 through Step 5 until the RBC Up Time/Count Time is within specification.

WBC Metering System Timing Adjustment


Follow the steps below for adjusting the timing of the WBC metering system:

Action

Prerequisite

Steps

Note
The RBC count times (T1 & T2) must be within specification before performing this
procedure.

Verify Count
Time

1. Remove and clean the WBC aperture plate as described in Section 9: Service and
Maintenance, Subsection: Cleaning/Replacing Aperture Plates of the
CELL-DYN 1800 Operator's Manual.

2. Run a background count and verify the count time displayed to the right of the WBC
histogram is 5.0 0.5 seconds.
Note
There is no count time adjustment procedure.
3. From the MAIN Menu, press [DIAGNOSTICS] followed by [RAW DATA].

4. Verify that the time displayed for WBC UPTIME is 1.8 to 2.2 seconds. (This time
represents T1 which is the time when the WBC valve 4-3 opens until the meniscus
reaches the upper detector. See WBC Metering Tube.)
o

If the WBC UPTIME specification is met, the procedure is complete,

If the WBC UPTIME specification is not met, go to Step 5.

5. Adjust the metering tube.


o

Raise the metering tube to increase the upper time.

Lower the metering tube to decrease the upper time.

6. Repeat Step 1 through Step 4 until WBC UPTIME/COUNT TIME is within specification.

Reference

VP-05 CMOS Setup Verification/Adjustment

Purpose

Provide instructions to verify and/or modify CMOS settings. Module CELL-DYN 1800

Materials Required None

Time

Action

00:10 min

Steps

Preparation

1. Power OFF the system.

Access CMOS Setup

1. Power ON the analyzer and press the Delete key, when prompted, to display

Utility Screen

the CMOS Setup Utility Screen.

1. Use the up/down arrow keys to select the STANDARD CMOS SETUP.
2. Press the Enter key.

3. Refer to the figure (H2_6006) and make any necessary adjustments to the
Standard CMOS Setup screen.

Verify/Modify
Standard CMOS
Setup

Note
Press the up/down arrow keys to select a setting and then press PgUp/PgDn
keys to modify.

4. Press ESC to exit the screen.

Reference

1. Use the up/down arrow keys to select the ADVANCED CMOS SETUP.
2. Press the Enter key.

3. Refer to the figure and make any necessary adjustments to the Advanced
CMOS Setup screen.
Note
Press the up/down arrow keys to select a setting and then press PgUp/PgDn
keys to modify.

4. Press ESC to exit the screen.

Verify/Modify
Advanced CMOS
Setup

1. Use the up/down arrow keys to select the ADVANCED CHIPSET SETUP.
2. Press the Enter key.

3. Refer to the figure and make any necessary adjustments to the Advanced
Chipset Setup screen.
Note
Press the up/down arrow keys to select a setting and then press PgUp/PgDn
keys to modify.

4. Press ESC to exit the screen.

Verify/Modify
Advanced Chipset
Setup

Note
The SAMSUNG LCD assembly is Type 8, while the TOSHIBA LCD assembly is Type 7.
1. Use the up/down arrow keys to select the POWER MANAGEMENT SETUP.
2. Press the Enter key.

3. Refer to the figure and make any necessary adjustments to the Power
Management Setup screen.
Note
Verify/Modify Power

Press the up/down arrow keys to select a setting and then press PgUp/PgDn

Management Setup

keys to modify.

4. Press ESC to exit the screen.

1. Use the up/down arrow keys to select the PERIPHERAL SETUP.


2. Press the Enter key.

3. Refer to the figure and make any necessary adjustments to the Peripheral
Setup screen.
Note
Press the up/down arrow keys to select a setting and then press PgUp/PgDn
keys to modify.

4. Press ESC to exit the screen.

Verify/Modify
Peripheral Setup

1. Press F10.

Save CMOS Settings


and Exit CMOS Setup

The message Save current settings and exit (Y/N)? displays.

2. Type Y and press Enter.


Note
Saves the CMOS settings, exits the CMOS Setup Utility and restarts the
computer.
Verification

1. Verify that the system boots up properly to the application software.

VP-06 Card Cage Backplane Test Points

Purpose

Identify test points on Backplane PCB. Module CELL-DYN 1800

Materials Required Digital Volt Meter (DVM)

Time

00:10 min

The following table lists the test points located on the backplane board and the figure illustrates the test points on the
backplane board.

Test

Function

TP1 +5V

Range

+5.00V 0.20V

TP2 +12V Analog

+12V 0.50V

TP3 -12V Analog

-12V 0.50V

TP4 +12V Digital

+12V 0.50V

TP5 -12V Digital

-12V 0.50V

Card Cage Backplane (Rear Side)

Ripple

Card Cage Backplane (Card Side)

LD4 +12VDC (DIG)

LD2 -12VDC (ANALOG)

LD5 -12VDC (DIG)

LD1 +5VDC

LD3 +12VDC (ANALOG)

Note
LEDs indicate voltages supplied to the board.

VP-07 Cable Distribution Module Test Points

Purpose

Identify test points on CDM. Module CELL-DYN 1800

Materials Required Digital Volt Meter (DVM)

Time

00:10 min

The following table lists the test points located on the Cable Distribution Module (CDM).

Location
Pin

Function Voltage Ripple


(J5 and J8)

Brown

Ground

< 0.05V

N/A

Blank

N/A

N/A

N/A

Orange

Ground

< 0.05V

N/A

Yellow

+14V

0.5V

N/A

Green

+28V

1.00V

N/A

Blank

N/A

N/A

N/A

Note
On the CDM, +28V is used to initially energize solenoids and +14V is used to keep them closed.
VP-08 Pre-Amplifier Module (PAM) Adjustment

Purpose

Adjust HGB self test and gain.

Module CELL-DYN 1800

Adjust RBC/PLT and WBC aperture currents.


Materials Required Digital Volt Meter (DVM)

Time

00:40 min

Flat Blade Screwdriver


Phillips Screwdriver
5-inch Jumper Leads
20K Ohm 1% Resistor
15K Ohm 1% Resistor

The Pre-Amplifier Module (PAM) is located on the upper right corner of the Flow Panel. Hemoglobin circuitry, and the
RBC/PLT and WBC aperture currents require verification and/or adjustment on this module. The HGB flow cell should be

cleaned before performing hemoglobin alignments (refer to Section 9, Subsection: Cleaning the HGB Flow Cell in the
CELL-DYN 1800 Operator's Manual).
Pre-Amplifier Module (PAM) Test Points
The following table lists the test points located on the PAM.

Location

Function

Voltage

TP5

Analog Ground

N/A

TP4

+100V

TP7

HGB Self Test/Gain Adjust +1.84V 0.02V (Self Test)

+100V 0.5V

+5.2V 0.2V (Gain Adjust)

Test points for the PAM are shown in the figure below.
Pre-Amp Module (PAM) Test Points

Follow the steps below to align the PAM:

Action

Steps

Reference

Open Upper Front Cover

1. Open the upper front cover.

Remove Lower Front Cover

1. Remove Lower Front Cover (RR-A1.02 Lower Front Cover).

Remove Top Cover

1. Remove Top Cover (RR-A1.03 Top Cover).

Remove Bezel

1. Remove the bezel (RR-A1.04 Bezel).

Remove Pre-amp Cover

1. Remove the pre-amp cover (RR-F1.01 PAM (Pre-Amplifier Module)).

HGB Self Test

1. Remove jumper JMP3 (lower right corner).


2. Press the touch plate to cycle the instrument and fill the HGB flow cell
with fresh reagent.
3. Connect the positive lead of the DVM to TP7.
4. Connect the ground of the DVM to TP5.
5. Adjust R14 to 1.84 volts 0.02 volts.
6. Verify the voltage at TP7.

7. Connect jumper JMP3 and verify voltage at TP7 returns to 5 volts.


Note
If voltage remains unchanged, the jumper may be in the wrong position.
Adjust HGB Gain

1. Press the touch plate to cycle the instrument and fill the HGB flow cell
with fresh reagent.
2. Verify the voltage at TP7.
3. Adjust R4 (HGB Gain Adjust) for 5.2 volts 0.2 volts.
4. Press the touch plate to cycle the instrument and fill the HGB flow cell
with fresh reagent.

5. Verify the voltage at TP7.


Note
If voltage does not meet specification, repeat Step 2 and Step 3.
Verify +100 Volts

1. Connect the positive lead of the DVM to TP4, and the negative lead to
TP5 (Gnd).

2. Verify 100 volts 0.5 volts.


Adjust RBC/PLT Aperture

1. Disconnect the RBC signal cable from J4.

Current

2. Connect a 20K Ohm 1/4 watt resistor across pins 4 and 5 of J4.
3. Connect the DVM across the resistor.
4. Run a background count.
5. Adjust R72 (RBC/PLT aperture current adjust) for 11.0 volts 0.05 volts.

6. Remove the resistor and reconnect cable to J4.


Adjust WBC Aperture

1. Disconnect the WBC signal cable from J3.

Current

2. Connect a 15K Ohm 1/4 watt resistor across pins 4 and 5 of J3.
3. Connect the DVM across resistor.
4. Run a background count.
5. Adjust R66 (WBC aperture current adjust) for 12.0 volts 0.05 volts.

6. Remove the resistor and reconnect cable to J3.


1. Reinstall the top cover, upper and lower front covers, bezel and pre-amp

Install Covers

cover.

The following table contains the specifications for the Pre-Amp Module alignment.
Pre-Amp Alignments

HGB SELF TEST

TP7

R14 +1.84V 0.02 V

HGB GAIN

TP7

R4

5.2 V. 0.2 V

RBC/PLT APERTURE CURRENT 20K Ohm Resistor R72 11.0 V. 0.05 V

WBC APERTURE CURRENT

15K Ohm Resistor R66 12.0 V. 0.05 V

VP-09 Signal Processor Module (SPM) Verification/Adjustment

Purpose

Adjust WBC, RBC, PLT Gain. and RBC Edit Ratio (RER) percentage (%). Module CELL-DYN 1800

Materials Required Digital Volt Meter (DVM)


Thin Flat Blade Screwdriver
Polystyrene Microspheres, 5.0 or 5.01 m
Polystyrene Microspheres, 3.31 m
Clean 50 mL Container

Note

Time

00:50 min

The Signal Processor Module (SPM) is located in the main electronics card cage. Alignment of the WBC, RBC, and
PLT gains are critical adjustments that must be verified and/or adjusted before instrument accuracy can be
established. It is important to maintain the adjustment order for this verification procedure. The adjustment order is:

Sequence

Activity

RBC Gain

RER Adjustment

WBC Gain

PLT Gain

Latex particles are used to perform these adjustments. The particles must be mixed vigorously before diluting to obtain
accurate results.
The Gain and RBC Cell Editing adjustments are performed in the Gain Adjust Mode, which allows multiple counts to be run
on the same sample. When Gain Adjust Mode is entered, whatever is in the Pre-Mix Cup is transferred to the WBC Cup and
5 mL of diluent is left undisturbed in the RBC Cup.
When performing RBC or PLT adjustments, 10 mL of diluent must be placed in Pre-Mix Cup before entering Gain Adjust
Mode to prevent air from being pulled into the von Behrens WBC Transducer during a count cycle.
Test points for the SPM are shown in the figure below:
SPM Test Points

RBC Gain

Action

Prepare Latex
Dilution

Steps

1. Pour 10 mL of diluent into the Pre-Mix Cup and add 1 drop of well-mixed 5.0 latex
particle solution to the 5 mL of diluent already in the RBC Mixing Chamber.
2. From the RUN screen, press [SPECIMEN TYPE].

3. Press Shift and # (simultaneously) to activate the Gain Adjust Mode.


Note
The message <PREPARING FOR GAIN ADJUST.> displays and WBC dilution is
transferred to the WBC Mixing Chamber. When the preparation cycle is complete,
the RUN screen displays.
Set RBC Gain

1. Press the Touch Plate to run a cycle.


Note
Always return to the RUN screen to run a cycle.
Note
When the procedure is completed, or if there is a need to exit the Gain Adjust Mode
while performing this procedure, such as constant clogs or improper dilution ratio,
press [SPECIMEN TYPE] followed by [PATIENT SPECIMEN].
Note
The RBC count should be between 3.5 and 4.5. If the count is outside this range,
modify the dilution ratio of the latex particles, exit Gain Adjust Mode and try again.
2. From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE].

3. Press [SMOOTHING OFF].


Note
The key changes to [SMOOTHING ON] and is highlighted, indicating the smoothing
function is ON.
4. Press [RBC HISTOGRAM].

5. Observe the lower part of the histogram data to determine if the channel number for
peak of 100 is Channel 98 1 channel.
o

If the specification is met, go to RER Adjustment.

If the specification is not met, adjust R27.


Note

Turning R27 clockwise decreases the channel number; turning R27


counterclockwise increases the channel number.

Reference

6. Return to the RUN screen and run another cycle.

7. Observe the channel number for peak count of 100.


o

If the gain is still outside of specification, repeat this process until the peak
count meets the specification (100 is in Channel 98 1 channel). Refer to
RBC Gain Adjustments (Example)
Note

Turning R27 clockwise decreases the channel number; turning R27


counterclockwise increases the channel number.
Continue with

1. Go to RER Adjustment.

RER Adjustment

RBC Gain Adjustments (Example)

RER Adjustment

Action

Determine

Steps

1. Verify that the dilution level on both the WBC

Adequate

and RBC transducers (left side) is above the

Dilution Amount

aperture (orifice) plates.


o

If the dilution level in transducers is


met, go to Determine Edit Ratio
Percentage.

If the specification is not met, go to


Step 2.

2. From the RUN screen, press [SPECIMEN


TYPE], then [PATIENT SPECIMEN].
3. Press the touch plate to run a cycle (exits Gain
Adjust Mode).
4. Pour 10 mL of diluent into the Pre-Mix Cup and
add 1 drop of well-mixed 5.0 m latex particle
solution to the 5 mL of diluent already in the
RBC Mixing Chamber.
5. In the RUN screen, press [SPECIMEN TYPE].

6. Press Shift and # (simultaneously) to activate


the Gain Adjust Mode.
Note
The message <PREPARING FOR GAIN ADJ.>
displays and diluent in the Pre-Mix Cup is
transferred to the WBC Mixing Chamber. When
the preparation cycle is complete, the RUN
screen displays.
Determine Edit
Ratio

1. Press the Touch Plate to run a cycle. Make a


note of the RBC results.

Percentage
Note
The RBC count should be between 3.5 and 4.5.
If the count is outside this range, adjust the
dilution ratio of the latex particles by adding
more diluent or latex to the RBC Mixing
Chamber.
2. Repeat Step 1 two more times for a total of
three (3) runs. Calculate the average of the
three RBC counts.

3. Refer to Cell Edit Table, to find the target value


for the following:

Reference

a. Use the average RBC calculated in


Step 2 to determine the Edit Ratio
Percentage that coincides with the
calculated average.
Note
Example: A count of 4 million relates to an edit
ratio of 28.0% 1.3.
Adjust RER

Cell Edit Table

Note
Use the edit ratio calculated in Determine Edit
Ratio Percentage above to perform the
following procedure:
1. From the MAIN Menu, press [DIAGNOSTICS]
followed by [RAW DATA].

2. Observe the RBC RER displayed on the screen


from the last cycle.
o

If the specification is met (see Cell Edit


Table), go to WBC Gain.

If the specification is not met, go to


Step 3.

3. Adjust R51.
Note
Turning R51 clockwise decreases the RER
percentage; turning R51 counterclockwise
increases the RER percentage.
4. Return to the MAIN Menu and run another
cycle.
5. Observe the RBC RER in the RAW DATA
screen.
6. If the ratio is still outside of specification, repeat
this process until the RBC Editing Ratio is within
the specified value.
7. Return to the MAIN Menu and press [RUN] to
access the RUN screen.
8. Observe the RBC Histogram.

9. The trailing edge of the histogram should be


straight with almost no humps. Refer to Editing
Ratio Displays.

Continue with

1. Go to WBC Gain.

WBC Gain

Editing Ratio Displays

WBC Gain

Action

Steps

Reference

Prepare Latex

1. From the [RUN] screen, press [SPECIMEN TYPE], then [PATIENT SPECIMEN].

Dilution

2. Press the touch plate to run a cycle (exits Gain Adjust Mode).
3. Obtain a clean container.
4. From the MAIN Menu, press [SPECIAL PROTOCOLS] followed by [MORE] twice to
display the [10 mL DISPENSE] key.

5. Press [10 mL DISPENSE] four times to dispense 20 mL of diluent into the container.
Note
Each 10 mL dispense requires key to be pressed twice.
6. Add 1 drop of well mixed 5.0 m latex particle solution into the 20 mL of diluent and mix
well.
7. Before entering the Gain Adjust Mode, use the prepared WBC latex dilution to fill the
Pre-Mix cup up to the level of the diluent inlet port.
8. From the MAIN Menu, press [RUN] followed by [SPECIMEN TYPE].

9. Press Shift and # (simultaneously) to activate the Gain Adjust Mode.


Note
The message <PREPARING FOR GAIN ADJ.> displays and WBC dilution is
transferred to the WBC Mixing Chamber. When the preparation cycle is complete, the
RUN screen displays.
Set WBC

1. Press the touch plate to run a cycle.

Gain
Note
Always return to the RUN screen to run a cycle.
Note
When the procedure is completed, or if there is a need to exit the Gain Adjust Mode
while performing this procedure, such as constant clogs or improper dilution ratio, press
[SPECIMEN TYPE] followed by [PATIENT SPECIMEN].
Note
The WBC count should be between 10.0 and 30.0. If the count is outside this range,
modify the dilution ratio of the latex particles, exit Gain Adjust Mode and try again.
2. From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE].

3. Press [SMOOTHING OFF].


Note
The key changes to [SMOOTHING ON] and is highlighted, indicating the smoothing
function is ON.
4. Press [WBC HISTOGRAM].

5. Observe the lower part of the histogram data to determine if the channel number for
peak of 100 is Channel 56 1 channel.
o

If the specification is met, go to PLT Gain.

If the specification is not met, adjust R22, and repeat Step 1 through Step 5.
Note

Turning R22 clockwise decreases the channel number; turning R22 counterclockwise
increases the channel number.
Continue with

1. Go to PLT Gain.

PLT Gain

WBC Gain Adjustments

PLT Gain

Action

Prepare
Latex

Steps

1. From the [RUN] screen, press [SPECIMEN TYPE],


then [PATIENT SPECIMEN].

Reference

Note
The PLT channel cannot be correctly

2. Press the touch plate to run a cycle (exits Gain Adjust


Mode).

3. From the MAIN Menu, press [SPECIAL


PROTOCOLS] followed by [MORE] twice. Place a
clean container under the Sample Probe and press
[10 mL DISPENSE] four times to dispense 20 mL of
diluent.
Note
Each 10 mL dispense requires key to be pressed
twice.
4. Add one (1) drop of well-mixed 3.31 m latex particle
solution and mix well.
5. Hold the diluted latex solution under the Sample Probe
and press [1/50 DILUTION] to aspirate for 1:50
dilution, while in the SPECIAL PROTOCOLS Menu.
6. Place a clean container under the Sample Probe and
press [1/50 DISPENSE] to dispense the 1:50 dilution.
7. Pull open Solenoid 2-5 (located directly under the left
side of the RBC/PLT Transducer) to drain the existing
solution in the RBC Mixing Chamber.

8. Pour the 1:50 dilution of 3.31 latex particles into RBC


Mixing Chamber.

Enter Gain
Adjust
Mode

1. Pour 10 mL of diluent into the Pre-Mix Cup (do not add


any latex particle solution).
2. From the RUN screen, press [SPECIMEN TYPE].

3. Press Shift and # (simultaneously) to activate the


Gain Adjust Mode.
Note
The message <PREPARING FOR GAIN ADJ.>
displays and WBC dilution is transferred to the WBC
Mixing Chamber. When the preparation cycle is
complete, the RUN screen displays.
Note
When running PLT counts, the lower front cover must
be on the instrument to prevent noise from interfering
with PLT results.
Adjust PLT

1. Press the touch plate to run a cycle.

Gain
Note

adjusted unless the RBC channel is at


the correct gain. The RBC channel
must be verified prior to adjusting the
PLT channel.

The PLT count should be between 150 and 350. If the


count is outside this range, adjust the dilution ratio of
the latex particles by adding more dilution or latex.
2. From the MAIN Menu, press [DIAGNOSTICS]
followed by [MORE].

3. Press [SMOOTHING OFF].


Note
The key changes to [SMOOTHING ON] and is
highlighted, indicating the smoothing function is ON.
4. Press [PLT HISTOGRAM].

5. Observe the lower part of the histogram data to


determine if the channel number for peak of 100 is
Channel 136 1 channel (refer to PLT Gain
Adjustments (Example).).
o

If the specification is met, the procedure is


complete.

If the specification is not met, adjust R41.


Note

Turning R41 clockwise decreases the channel


number; turning R41 counterclockwise increases the
channel number.
6. Return to the RUN screen and run another cycle.
7. Repeat Step 2 through Step 5.
8. To exit Gain Adjust mode, go to the RUN Menu and
press [SPECIMEN TYPE] followed by [PATIENT
SPECIMEN]
9. Run two to three background cycles.

10.Verify acceptable background results (refer to


Specifications & Checklist).

PLT Gain Adjustments (Example)

VP-10 Diluent and Sample Verification/Adjustment

Purpose

Verify diluent dispense to maintain proper dilution ratios. Module CELL-DYN 1800

Materials Required 10 mL Graduated Cylinder

Time

00:15 min

40 L Micropipettes
100 L Micropipettes
Clean 50 mL Container

WARNING
Potential Biohazard

This procedure consist of both Diluent and Sample adjustment that can be accessed with the following links:

Diluent Volume Verification


Sample Volume Verification

To minimize problems like coincidence passage, the CELL-DYN 1800 uses two different dilution ratios of whole blood to
diluent. The ratio for WBC/HGB is 1:285; the ratio for RBC/MCV/PLT is 1:12,801. The following procedure is used to verify
the diluent dispense to maintain proper dilution ratios and thereby optimize instrument performance.
Diluent Volume Verification

Action

Steps

Verify

1. From the MAIN Menu, press [SPECIAL PROTOCOLS] followed by [MORE] twice.

Diluent

2. Place an empty 10 mL graduated cylinder under the Sample Probe and press [10 mL

Volume

Reference

DISPENSE] twice to dispense 10 mL of diluent.


3. Verify a volume of 10 mL 0.2 mL.
4. Place an empty 10 mL graduated cylinder under Sample Probe and press [1/50
DILUTION]. Once the probe has returned to the aspirate position, press
[1/50 DISPENSE] to dispense.

5. Verify a volume of 5 mL 0.1 mL.


Note
The volume dispensed is under direct computer control. If the volume is out-of-range, the
Dispenser and Stepper Motor drive circuitry must be repaired. (Refer to RR-E1.02
Diluent Syringe Driver Assembly.)

Sample Volume Verification

Action

Steps

Sample Volume

1. Remove the 1/32" silicone tubing attached to the top of the sample probe.

Verification

2. Attach a 100 microliter pipette to the silicone tubing.

3. Place the tip of the pipette on the bottom of a small container and press [10 mL
DISPENSE] twice.
Note
Keep the tip of the pipette submerged when dispensing.

Reference

4. Take the pipette out of the container and wipe any drop from the end of the pipette,
being careful not to wick any liquid from the end.
5. Press [1/50 DILUTION].
6. Verify that the column of liquid is no more than 1/16 inch above or below 100
microliter mark on the pipette.
7. Replace the 100 microliter pipette with a 40 microliter pipette.
8. Place the tip of the 40 microliter pipette on the bottom of a small container and press
[1/50 DISPENSE].
9. Take the pipette out of the container and wipe any drop from the end of the pipette,
being careful not to wick any liquid from the end.
10. Press [1/250 DILUTION].

11.Verify that the column of liquid is no more than 1/16" above or below 40 microliter
mark on the pipette.

Remove Pipette

1. Place a waste container under the tip of the pipette and press [1/250 DISPENSE].

2. Remove the pipette and re-attach the silicone tubing to the sample probe.
Note
The volume aspirated is under direct computer control. If the volume is out-of-range,
the Sample Syringe and Stepper Motor Drive must be repaired. (Refer to RR-E1.04
Sample Syringe Driver Assembly.)

VP-11 Stepper Motor Power Test and Verification

Purpose

Verify stepper motor power. Module CELL-DYN 1800

Materials Required None

Action

Test Stepper
Motor Power

Time

00:30 min

Steps

1. From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE] three times.
Then press [SERVICE DEC CODE].
2. Type in 128 from the keyboard and press Enter.
3. The test runs for approximately 30 seconds.
4. When the test is complete, the results are displayed on the screen.

5. Compare the results displayed on the screen with the nominal values listed in Motor

Reference

Power Specifications. Ensure that all values fall within the specified ranges.

Motor Power Specifications

VP-12 Sample Probe Alignment Check

Purpose

Check Probe Alignment.

Module CELL-DYN 1800

Materials Required Standard Set of Hex Wrenches Time

Action

Check

Steps

1. From the MAIN Menu, press

Probe

[DIAGNOSTICS], followed by [MORE]

Alignment

twice, then [PROBE HOME].

2. Observe the wash block in relationship


to the probe tip. Verify that the tip of the
probe is between the waste removal
channel and the top of the countersink,
located at the bottom of the wash block.
Note
Proper probe tip location is essential for
proper cleaning of the probe, and
prevents carryover and CV problems.
3. If alignment is required, loosen the set
screw on the probe collar and raise or
lower probe until proper height is

00:10 min

Reference

achieved.
4. Retighten set screw on probe collar.

5. Press PROBE DOWN to exit.

VP-13 Serial Transmit to LIS Verification

Purpose

Verify operation of serial port.

Materials Required Laptop computer w/Windows 2000

Module CELL-DYN 1800


Time

00:40 min

Copy of CELL-DYN 1800 Interface Specification (Latest Revision)


Nine (9) Pin Null Modem Serial Cable

This verification procedure is used to verify the operation of the serial port (COM 1) that is used for communication with a
Laboratory Information System (LIS). This procedure only verifies the transmit function.
A 9 pin, "null modem", serial cable is needed for this procedure.
Note
The screens shown in this procedure are given for example only. Each model instrument has slightly different field
structure and content to the count data message that is downloaded. Consult the applicable interface specification
document for details.

Action

Create New
Connection

Steps

1. Place the laptop near the instrument and


connect the null modem serial cable to
COM 1 on the right side of the instrument.

Reference

Connect the other end to COM 1 on the


laptop.
2. From the Windows 2000 desktop, use the
mouse to click on Start.

3. Highlight Programs, Accessories,


Communications, and click Hyper
Terminal.
The Connection Description window
displays.
4. In the Name field, type in the filename
sertest1.

5. Select the first icon and click the OK


button.

Select

1. The Connect To window displays:

Connection

2. At the bottom, in the box Connect


using:, use the mouse to select COM 1.
Note
All the other boxes are blocked (gray).

3. Click the OK button.

Configure

1. The COM1 Properties window displays:

COM1

2. Configure the Bits per second: field to


match the Baud Rate in the CELL-DYN
SETUP menu.
3. Verify that the Data bits, Parity and Stop
bits are configured according to the
CELL-DYN SETUP menu.

4. In the CELL-DYN SETUP menu, verify

the CTS enabled setting:


o

If CTS enabled is off set Flow


control: to Xon/Xoff.

If CTS enabled is on set Flow


control: to Hardware.

5. Click the OK button.

sertest1
Active

1. The test session window named sertest1


is now active.
Note
If the message "Unable to open COM1.
Please check your port settings"
displays, there may be another device
assigned to the COM1 port, such as the
modem. Contact the local area IS
administrator for assistance with resolving
device interrupt conflicts.

2. Compare the results to the interface


specification. The screen is shown for
example only. Transmit specimen data as
many times as desired.

Transmit
Data

1. From the MAIN Menu, press


[DATALOG], followed by [TRANSMIT
DATA].
2. Enter starting and ending sequence
numbers of data to be transmitted.

3. Compare the results to the

CELL-DYN 1800 Interface Specification.


Note
The screen below is shown for example
only.

4. If desired, click on File then Print to


obtain a printout. When testing is
complete, click on File then Save. After
saving, click on Call, then Disconnect,
then exit the window by clicking the X.
Close out the Hyper Terminal window by
clicking the X.

VP-14 Hard Disk Drive Setup and Verification

Purpose

Provide instructions to verify/modify CMOS Settings, partition and format

Module CELL-DYN 1800

replacement hard disk drives


Materials

DK, Drive, Hard, IDE (8200570701)

Required

DKS (C) SRV ABBOTT CD3200 (8938070001)

Time

00:30 min

CELL-DYN 1800 Installation Disk


Phillips Screwdriver

Action

Preparation

Steps

Note
If replacing an existing customer hard disk drive, print copies of all customer
settings, quality control data files, and the data log if the drive is accessible. It
may not be possible to access this data if the hard disk drive is corrupt or
defective.
1. Power OFF the system.

2. Verify jumper setting is set to Master according to Jumper Configuration Table


on hard disk drive.

Install Hard Drive

1. Install the new hard drive using the existing hardware and cables (refer to
RR-F1.04 Hard Disk Drive).

Reference

Access CMOS Setup


Utility Screen

Verify CMOS Settings:


Standard CMOS Setup

1. Power ON the analyzer and press the Delete key, when prompted, to display
the CMOS Setup Utility Screen.

1. Use the up/down arrow keys to select the STANDARD CMOS SETUP.
2. Press the Enter key.
3. Set Pri Master to Auto.

4. Set 32 Bit Mode to ON.


Note
Pri Slave, Sec Master and Sec Slave show Not Installed.

5. Press ESC to exit the screen.


Verify CMOS Settings:

1. Use the up/down arrow keys to select the ADVANCED CMOS SETUP.

Advanced CMOS Setup

2. Press the Enter key.


3. Set Quick Boot to Enabled.
4. Set 1st Boot Device to Floppy.
5. Set 2nd Boot Device to IDE-0.
6. Set 3rd Boot Device to Disabled.
7. Set 4th Boot Device to Disabled.

8. Press ESC to exit the screen.


Verify CMOS Settings:

1. Use the up/down arrow keys to select the ADVANCED CHIPSET SETUP.

Advanced Chipset

2. Press the Enter key.

Setup

3. Set LCD CRT Selection to Simultaneous.


4. Set LCD Type to Type 8.

5. Press ESC to exit the screen.


Verify CMOS Settings:

1. Use the up/down arrow keys to select the POWER MANAGEMENT SETUP.

Power Management

2. Press the Enter key.

Setup

3. Set Power Management/APM to Disabled.


4. Set CPU Critical Temperature to 60C/140F.

5. Press ESC to exit the screen.


Verify CMOS Settings:
Peripheral Setup

1. Use the up/down arrow keys to select the PERIPHERAL SETUP.


2. Press the Enter key.
3. Set OnBoard FDC to Enabled.

4. Press ESC to exit the screen.


Save CMOS Settings
and Exit CMOS Setup

1. Place the DKS (C) SRV ABBOTT CD3200 (8938070001) (service disk) into
the floppy drive.

2. Press F10.
The message Save current settings and exit (Y/N)? displays.

3. Type Y and press Enter.


Note
Saves the CMOS settings, exits the CMOS Setup Utility and restarts the
computer.
Create DOS Partition

1. At the A:\> prompt, type FDISK and press Enter.


2. At the FDISK Options menu, enter choice 1 and press Enter.

3. At the Create DOS Partition or Logical DOS Drive menu enter choice 1 and
press Enter.
Note
If you get the message Primary DOS Partition already exists, press Esc to
exit. Then go to Format Hard Drive, Step 1.
The message Do you wish to use the maximum available size for a
Primary DOS Partition and make the partition active (Y/N)? displays.

4. Type Y, and press Enter.


Note
Uses the maximum size for the hard drive DOS partition and makes it active.

5. When the screen displays System will now restart. Insert DOS system
diskette in drive A:. Press any key when ready, press Enter.
The system reboots and displays the A:\ prompt.
Format Hard Drive

1. At the A:\> prompt, type FORMAT C: /S and press Enter.


The message WARNING: ALL DATA ON NON-REMOVABLE DISK DRIVE
C: WILL BE LOST! Proceed with Format (Y/N)? displays.

2. Type Y, and press Enter.


The message Volume label (11 characters, ENTER for none)? displays.

3. Press Enter for None.


Install DOS and System

1. At the A:\> prompt, type INSTALL and press Enter.

Software
Note

Installs DOS and system files.


2. When the system installation is complete, remove the service disk and insert
the CELL-DYN 1800 Installation disk into the floppy drive.

3. Type Install and press Enter.


Note
When the installation is complete, the message "Power off the instrument
and remove diskette. Power on the instrument." displays.

4. Remove the CELL-DYN 1800 Installation disk and power OFF the instrument.
Verification

1. Power ON the instrument

2. Verify that the system boots up properly to the application software.


Note
During initial power up after a hard disk drive installation, DO NOT turn off the
instrument while the Data Log is being created. This corrupts the Data Log.

3. If printouts are available, re-enter the data from the printouts. If not, perform
instrument calibration procedure and verify control recovery.

VP-15 Software Installation/Upgrades

Purpose

Install or Upgrade Software

Module CELL-DYN 1800

Materials Required Disk, installation (8938144001) Time

Action

00:10 min

Steps

Software

1. With the CELL-DYN instrument ON, go to the DIAGNOSTICS Menu.

Installation/Upgrade

2. Press ESC on the PC keyboard. A warning message displays on the screen.


3. Press Alt and x (simultaneously). The following DOS prompt displays:
C:\CD1800>.
4. Insert the CELL-DYN 1800 Installation disk into Drive A.
5. Type a: and press Enter.

Reference

6. Type install and press Enter.


Note
When the installation is complete, the message "Power off the instrument
and remove diskette. Power on the instrument." displays.
7. Remove the diskette and power OFF the instrument.

8. Power ON the instrument and allow it to initialize.


Note
During initial power up after a hard disk drive installation, DO NOT turn off the
instrument while the Data Log is being created. This corrupts the Data Log.