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FLOW CYTOMETRY

DEFINITION
Flow cytometry is a technology that

simultaneously measures, and then analyses


multiple physical characteristics of single
particles, usually cells, as they flow in a fluid
stream through a beam of light, usually a
laser.

BASICS OF FLOW CYTOMETRY


What can a flow cytometer tell us about a cell
Its relative size (Forward ScatterFSC)
Its relative granularity or internal complexity

(Side ScatterSSC)
Its relative fluorescence intensity
(FL1, FL2, FL3, and FL4)

Components of FLOW
CYTOMETRY:

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