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ProteinwithtwositesforBinding.

Twotypesofligands canbind.

#1 =monovalent
#1
monovalent

#2 =bivalent
#2
bivalent

1)Drawallofthepossiblestates.
2)WritedownalleightdistincttermsintheMolecularPartitionFunction
2)
Write down all eight distinct terms in the Molecular Partition Function
usingbindingconstantsK1 ,K2, andalsoK3ifneeded.
=1+2K1 c1 +
3)Calculate
3)
Calculate thefractionalsaturationof#1bindingtotheProtein:
the fractional saturation of #1 binding to the Protein:
1 =(c1 /)d /dc1
4)Sketch1 forc2 =0.
5)Sketch1 inthesameplotforc2 =c1 andK1 =0.5K2 =0.5K3, whichmodelsa
relativelystrongbindingbivalentcontaminant.