Laus and company On July 15 of last year, a 38 year old female found she was 2 weeks pregnant with

her 2nd baby. On her first pre-natal check-up, she failed to reveal to her new obstetrician that on the last trimester of her pregnancy for her first baby, she was diagnosed with gestational diabetes. Although her pregnancy was uncomplicated, her blood glucose level peaked a week after her delivery. Her blood glucose levels remained elevated until her baby was about 5 months of age (with the levels becoming lower and lower each month) afterwhich, she stopped coming in for monthly check-ups. Up until January of the present year, with the exception of extremely painful and enlarged varicose veins in her genitals, her routine pre-natal visits and routine laboratory work-ups (CBC, U/A and F/A) and are all normal. She has been gaining a noticeably greater weight relative to what is expected in her condition and the doctor is worried that her varicose veins might rupture if she continues to gain more weight until she reaches full-term. Also, if her veins continue to enlarge, she might bleed to death during delivery. The doctor requested for an amniotic fluid analysis. RESULTS: Absorbance at 350 nm ± 0.25 Absorbance at 425 nm ± 0.40 Absorbance at 475 nm ± 0.32 Absorbance at 550 nm ± 0.15 PG ± absent Lecithin ± 4.7 mg/dL Sphingomyelin ± 2.3 mg/dL Questions: Absorbance at 400 nm ± 0.30 Absorbance at 450 nm ± 0.50 Absorbance at 500 nm ± 0.20 Absorbance at 600 nm ± 0.10

1. In what week of gestation is amniotic fluid collection and analyze, when testing for chromosome A. 14rd week B. 15th week C. 17th week D. 16th week E. None of the above 2. What is the principle involved in determining the quantitative measurement of L/S ratio? A. Thin Layer Chromatography B. Fluorometry C. Reflectance Photometry D. Flame Emission Photometery E. None of the above 3. What are the two process involved in amniocentesis? A. Transabdominal & Urethral B. Urethral & Spinal C. Vaginal & Intravenous

D. TRansabdominal & Vaginal E. Either A & C 4. It is used to determine maturity of fetal lungs in relation to gestational period A. Fetal Lung Maturity Assessment B. Fern Test C. Biochemical Assay D. Nitrazine paper test E. None of the above 5. Positive Result for Foam Stability Index A. Stable foam ring B. Unstable foam ring C. No ring formation D. Either A & B E. None of the above 6. What body fluid may be used to diagnose the patients¶ condition having a gestational diabetes? A. Synovial fluid B. Amniotic fluid C. Pericardial fluid D. Either of the 3 E. None of the above 7. Which parameters may be affected if the specimen is contaminated with blood? A. Phosphotidyl glycerol B. Lecithin C. Sphingomyelin D. Urobilinogen E. All of the above 8. This test differentiates urine from Amniotic fluid A. Fern test B. Foam stability Index C. Kleihauer ± Bethke Test D. Firm Test E. None of the above 9. What are some of the cause of oligohdramnios? A. Fetal demise B. intrauterine growth retardation (IGR), C. Premature rupture of membranes D. Potter sequence E. All of the above

10. What result may be obtained from a fetal ± blood contamination of bilirubin? A. High B. Falsely increased C. Falsely decreased D. Have no effect E. None of the above

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