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III

OBSERVATION METHOD
A. Time and place
Day / Date
Time

: Thursday / April 23th 2014
: at 08.00 a.m – 10.30 a.m

: Biology Laboratory, 3rd floor east side Mathematics and
Science Faculty, State University of Makassar.
B. Equipments and Materials
Place

1. Equipments

a. Pastle and mortar

d. tweezers

b. Centrifuge

e. Stopwatch

c. Spatula

f. Comboplate/test tube

2. Materials

a.

Banana

b. Cucumber

c.

Apple

d. Tomato

e. pH7 buffer

f. 1% H2O2 solution

C. Work Procedure
a. Grid the plant in a mortar with a

b. Scrape the paste into a centrifuge

small amount of pH7 buffer

tube, and spin high speed until a pellet

c.transfer the supernatant to a clean

is formed
d. Dip a dry disc of filter paper in the

labelled centrifuge tube.

extract and blog briefly on a sheet of

e. Drop the disc into a solution

filter paper
f. note the time taken for the disc to
rise back to the surface and then
remove the disc