..

..

SensibleTAB

positive-sense RNA

7
8
9
10

.. 2557

12-25

..

26-33

.. 2557

34-37

Sensible TAB

38-43

positive-sense RNA

.. 2556

44
45
46
47
48
49-52
52-53
54-59
60

.. 2526



.. 2534
35

.. 2543



(middle income country)

.. 2557

.. 2557

1 4
30




(.)






.. 2557

(. )

(Middle Income Trap)

(Niche Market)


..

(. )

2557



.



Surrogate Broodstock
..2557
(Slurry Metal Casting Technology) ..


..2557
38 .
.

..2557



..2557

( . )

10

11

Dr. Varin Tanasomwang

Fisheries Management Expert
Department of Fisheries

1. 9.
2.
10.
3.
11.
4.
12.
5.
13.
6. 14.
7.
15.
8.
16.

12

Abstract
Thailand is a leader country in fishery production contributing the important
sources of protein food either for domestic consumption or exportation to earn foreign
income. Although the fishery production is mainly from sea capture, its production has
been declined continuously from 2,631,700 metric tons in 2001 to 1,610,400 metric tons
in 2011 as the result of over fishing. Aquaculture plays a significant role in increasing
production to meet the demand for fish and other fishery products. It is our efforts to
develop technology for coastal aquaculture of economically important marine species
including the blue swimming crab (Portunus pelagicus), 3 species of high value of groupers
namely giant grouper (Epinephelus lanceolatus), leopard coral trout or blue spotted
grouper (Plectropomus leopardus) and tiger grouper (Epinephelus fuscoguttatus), Asian
seabass (Lates calcalifer) and Pacific whiteleg shrimp (Litopenaeus vannamei). The
technology development offers an opportunity for improving food security and food
safety.
Technological development of seed production and culture of blue swimming
crab has been covered the suitable conditions for hatching of crab eggs from abdomen of
berried females, rearing of crab from newly hatched larvae in zoea I to young crab stages
(6-7 days after metamorphosed into crab stage) and the production of zooplankton as
feed for the larvae and young crab. Preliminary investigation in the feasibility of using
artificial feed for blue swimming crab culture in the earthen ponds has been established.
Development of the prototype for commercialized seed production of 3 high
valued species of groupers, giant grouper, leopard coral trout or blue spotted grouper
and tiger grouper, has been in progress. The technologies are included the management
of broodstock and the technical and environmental management for rearing of fish
larvae. In addition, diagnosis for the causative agent of the viral nervous necrosis (VNN) in
groupers, a devastating disease of cultured marine fish worldwide, by using polymerase
chain reaction (PCR) technique has been implemented. This technological development
for groupers seed production is under 5 year project from 18 August 2010 to 17 August
2015. Up to June 2014, 3-5 inches juveniles of the 3 species of groupers were produced
and sold to the farmers for culture especially in cages. The grouper seeds revenue
accounting for 13,697,800 baht is out of 21,840,000 baht that project has committed with
grant source, Agricultural Research Development Agency (Public Organization), ARDA.
In Asian seabass and Pacific whiteleg shrimp, genetic improvement for better
culture performance and tolerance of these species ensures the increasing productivity
and sustainability. Oxygen depletion tolerance trait has been one of the target for Asian
seabass while low-salinity tolerance and white spot syndrome virus (WSSV) resistance
are targeted traits for the Pacific whiteleg shrimp. Moreover, surrogate broodstock
technology has been developed by transplanting spermatogonia or oogonia isolated
from giant grouper into tiger grouper recipients. Development of the monitoring methods
for leucomalachite green (LMG) residue using the ELISA test kit in farmed fish and shrimp
has also been undertaken. These activities are advanced ongoing.
13

19 2500

2520
Miyazaki 2529
Hiroshima 2532
2520 3

2523 4


2533 5



2546

2552

2556

- 2536

-
2545
Portunus pelagicus Linnaeus, 1758

2545
-
4 2548

- SMART TEAM 2549

- SMART TEAM 2551

2544
2,631,700 1,610,400
2554

2544-2554
534,500 817,000

3
/

(.) (
) (.) Japan International Cooperation
Agency (JICA)

14

3-4


2-4

3-5

79.56%
70.57%

young crab


zoea I 30-32 ppt 25 ppt megalopa
megalopa
megalopa
pH (alkalinity)
young crab 0.3-0.7 . 3 0.21-0.22 /
(mass production)
15

(Brachionus plicatilis) (Moina macrocopa)
Chlorella

Chlorella

- () 15
- (pH) 9

Chlorella -
() 349.44 /


(Artemia spp.)
Chlorella Chlorella

206.49 /


2
(9.47+0.37% 8.34+1.57%
)

16

4-6

3 (Epinephelus
lanceolatus) (Plectropomus leopardus)
(Epinephelus fuscoguttatus)

Viral Nervous Necrosis (VNN)

nitrate
20%

1
17 -methyltestosterone
2557
4.6

17 -methyltestosterone


3.0-3.5 2.5 /
1
3
17

VNN
Polymerase Chain Reaction (PCR)

VNN

VNN




VNN



n-3 HUFA

2.50-6.50 . ( 1-12 ) 14 /

7 /

14 /
6.5 .-1 ( 13-40 )
1-3 ( 40-80 )
1





1-12
13-30 200 600-800 ./

14-30

2 (
329.75+68.52 - 423.04+49.40 Lux)
1 (
226.59+80.60 - 235.20+57.38 Lux)
( 188.98+13.88209.43+1.70 Lux)

n-3 HUFA

3 (A, B C) (Schizochytrium
limacinum)
3
A


A B
C

A DHA EPA
ARA

18

2516

91
DNA microchip (mass spawning)
DNA


3

................................................................................

Surrogate broodstock

17 (donor) (germ cells)
(recipient) 5.5 .
(microinjection)
30
germ cells
( ) 1.2-20%
1.2-15% 45

(colonization)

3

19

White
Spot Syndrome Virus (WSSV) Infectious Hypodermal and
Hematopoietic Necrosis Virus ( IHHNV) Taura Syndrome
Virus (TSV) 5




WSSV

.........................................................................................................................

..........................................................

.....................................................................

Malachite green (MG)



MG
leucomalachite green
(LMG)

LMG ELISA kit

HPLC LC-MS

MG

.......................................................................

20

10-34% (
)

1 . (mass production)

()

3,500,000
3,274,300

3,000,000

2,500,000
2,149,149

2,000,000

1,500,000

1,269,810

1,000,000
604,470

401,340
500,000 422,000
200,000

2549 2550 2551 2552 2553 2554 2555 /

1-3 .

2549-2555

21

. 28,051,275 5 18
2553 17 2558
3


21,840,000
3 3-5
2557
13,697,880
.
3
10

:
23 2557

.
5-8 2557

28-29 2557
1

22

23

3
5
398,000 5
21,840,000
3
161,500,000


3 3
/

(surrogate broodstock)

5%

24

. 2545. (Portunus pelagicus

Linnaeus) .
. 55(4): 319-323.
, ,
. 2547. (Portunus
pelagicus Linnaeus, 1758)
. 35/2547.
, ,
, . 21 .
, .
2547. (Portunus pelagicus
Linnaeus, 1758) .
36/2547. ,
, ,
. 17 .
. 2548.
(Portunus
pelagicus Linnaeus, 1758) .
1/2548.
, ,
, . 14 .
. 2548.
(Portunus
pelagicus Linnaeus, 1758) .
10/2548.
, , ,
. 23 .

25

, .
2548.
(Portunus pelagicus Linnaeus, 1758)
. 19/2548.
,
, , . 31
.
. 2548.
(Portunus pelagicus
Linnaeus, 1758) .
30/2548. ,
, ,
. 20 .
. 2548.
(Portunus pelagicus Linnaeus, 1758)
.
31/2548.
, ,
, . 19 .
,
. 2549.
(Portunus pelagicus Linnaeus, 1758)
. 24/2549.
,
, , . 38 .
.

..

Assoc. Prof.
Dr. Jessada Wannasin
..
2548
.. 2550
.. 2553

2552
2550
2553

.. 2550

20 2520 2550
Thainox Metallurgy
5 Award 2549
..
.. 2538
-- 27 16
29
(Solidification Processing) Acta
12 Materialia 3 Scripta Materialia 3
Williston Northampton School Massa- 2
chusetts 1 1
Case Western Reserve University Ohio 2
Materials Science 3 3
..
and Engineering .. 2543 Summa Cum Laude 2552
Massachusetts Institute of
Technology Massachusetts
.. 2547 Materials Science and
Engineering
Postdoctoral Research Associate
Case Western Reserve University Ohio

26

Abstract

Several components in the automotive, electronic, agricultural, or toy industries are produced in a high
volume by high-pressure die casting. However, this process still has several limitations and disadvantages such
as long cycle time, high energy cost, high water treatment cost, and high reject rates from porosity. These
limitations result in inefficiency of the process and increased production costs. For the last 40 years, a solution
to this problem has been focused on applying a semi-solid metal forming process. In this process, a metal is
converted into semi-solid metal and formed in this ice-cream state. Significantly improved quality can be
obtained with semi-solid metal forming, but the process requires a lot of know-how and investment. Thus, it
has not been widely applied in the casting industry yet. It is, therefore, the objective of this new technology
to solve the limitations and disadvantages of conventional die casting and semi-solid metal forming to offer
cost savings and improved quality in the metal casting industries. This new casting technology, called Gas
Induced Superheated Slurry (GISS) Technology, is a metal forming process which casts a metal slurry having
a temperature above the liquidus temperature and then forms it into shapes. The slurry contains a large
number of micron-size solid particles in a uniform suspension in the liquid phase. The slurry can then flow
in a less turbulent manner, resulting in less porosity and improved quality. Key benefits of the slurry casting
technology include reduction of gas and shrinkage porosity, die life extension, cycle time reduction, energy
consumption reduction, and reject reduction. The GISS Technology is being commercialized in the industry
through GISSCO Company Limited, which was founded by Dr. Jessada Wannasin, Prof. Merton Flemings, and
co-workers in 2009. The GISS units, a product of the GISS Technology, are being used for automotive and
electronic applications in Thailand and Korea, and will soon be used in the US and Japan.

(Liquid
Casting)
(Cycle Time)

(Semi-solid
Casting)
30-50%
(Turbulent flow) (Shrinkage)






(Slurry Metal Casting)
27

1
()
()

10% ( 1)

(Turbulent Flow)
(shrinkage)




2
3

3 GISS Unit

2 Step 1 Step 2
Step 3 Step 4
(1 , 2 , 3 , 4 , 5 )

28

..........................................................................
1)



4

2)




5

3)

5-10
2-3

..........................................................................

4)




20-30%

5)

20-30%

6)

..........................................................................

4 () ()

5 () ()

29

Summit Steering
Wheel

Reject
(Cycle Time)


Summit Steering Wheel
6-7

Summit Steering Wheel

10 1
2
30%
5%

6
()
() 150,000

7 X-Ray
()
()

.

Exclusive License 6
2555

GISS Unit

1) P.C.S. Die Casting
NIJ Level 3
2) Summit Steering Wheel

3)

Metal Matrix Composite

7075
1) SeA Mechanics
8
2) Thixomat

30

Cyclic 2,000,000
9

1) Method to Prepare Metal Structure Suitable for Semi-Solid Metal Processing. International Publication Number: PCT/US2007/002503; Inventors:
Wannasin, Jessada; Martinez, Raul A.; Flemings, Merton C.
2) Process for Preparing Molten Metals for Casting at a Low to Zero
Superheat Temperature. International Publication Number: PCT/
TH2014/000025; Inventors: Wannasin, Jessada; Flemings, Merton C.


1)
1001002009 24 .. 2553
2)
1001001556 7 .. 2553
3)
1101001152 14 .. 2554
4)

1203000208 1 2555

28

50

31

30
1) Canyook R, Wannasin J, Wisuthmethangoon S, Flemings MC. Characterization of the Microstructure Evolution of a Semi-solid Metal Slurry
during the Early Stages. Acta Materialia, 60 (2012), pages 3501-3510
[ISI Journal Impact Factor =3.941]
2) Janudom S, Wannasin J, Basem J, Wisutmethangoon S. Characterization of flow behavior of semi-solid slurries containing low solid
fractions in high-pressure die casting. Acta Materialia, 61 (2013), pages
6267-6275 Acta Materialia, 61 (2013), pages 3897-3903 [ISI Journal
Impact Factor =3.941].
3) Chucheep T, Wannasin J, Canyook R, Rattanochaikul T, Janudom
S, Wisutmethangoon S, Flemings MC. Characterization of Flow Behavior of Semi-Solid Slurries with Low Solid Fractions. Metallurgical
and Materials Transactions A, 44 (2013), pages 4754-4763 [ISI Journal
Impact Factor =1.627].
4) Wannasin J. Applications of Semi-Solid Slurry Casting Using the
Gas Induced Semi-Solid Technique. Solid State Phenomena, 192-193
(2013), pages 28-35.
5) Canyook R, Wannasin J, Wisuthmethangoon S, Flemings MC. Characterization of the Microstructure Evolution of a Semi-solid Metal Slurry
during the Early Stages. Acta Materialia, 60 (2012), pages 3501-3510
[ISI Journal Impact Factor =3.755]
6) Wisutmethangoon S, Thongjan S, Mahathaninwong N, Plookphol T,
Wannasin J. Precipitation hardening of A356 Al alloy produced by gas
induced semi-solid process. Materials Science and Engineering A, 532
(2012), pages 610-615. [Journal Impact Factor = 2.101]
7) Mahathaninwong N, Zhou Y, Babcock SE, Plookphol T, Wannasin
J, Wisutmethangoon S. Creep rupture behavior of semi-solid cast
7075-T6 al alloy. Materials Science and Engineering A, 556 (2012),
pages 107-113.
8) Mahathaninwong N, Plookphol T, Wannasin J, Wisutmethangoon S.
T6 heat treatment of rheocasting 7075 Al alloy. Materials Science and
Engineering A, 532 (2012), pages 91-99. [Journal Impact Factor = 2.101]
9) Kovac P, Sidjanin L, Rajnovic D, Savkovic B, Wannasin J. The microstructure influence on the chip formation process of Al-Cu alloy cast
conventionally and in semi-solid state. Metalurgija, 51 (2012), pages
34-38. [Journal Impact Factor = 0.35]
10) Kongiang S, Plookphol T, Wannasin J, Wisutmethangoon S. Effect
of the two-step solution heat treatment on the microstructure of
semisolid cast 7075 aluminum alloy. Advanced Materials Research,
488-489 (2012) pages 243-247.
11) McCuiston R, Ngernbamrung S, Dateraksa K, Sujirote K, Wannasin
J, Sungkapun T. Fabrication of high volume fraction SiCp / A1 metal
matrix composites. Ceramic Engineering and Science Proceedings
(2011), page 71.
12) Sujirote K, Dateraksa K, Ngernbamrung S, McCuiston R, Sungkapan
T, Wannasin J. Preceramic-polymer-bonded SiC preforms for high
volume fraction SiCp/Al composites. Ceramic Engineering and Science
Proceedings (2011), page 189.
13) Pannaray S, Wisutmethangoon S, Plookphol T, Wannasin J. Microstructure evolution during solution heat treatment of semisolid
cast 2024 aluminum alloy. Advanced Materials Research, 339 (2011),
pages 714-717.
14) Mahathaninwong N, Wisutmethangoon S, Plookphol T, Wannasin J.
Influence of solution heat treatment on microstructures of semisolid
cast 7075 aluminium alloy. Advanced Materials Research 339 (2011),
pages 371-374.
15) Chucheep T, Canyook R, Rattanochaikul T, Janudom S, Wisutmethangoon S, Wannasin J. A fluidity study of semi-solid rheo-slurry

of AC4C aluminum alloy in gravity sand casting. Advanced Materials

Research, 337 (2011), pages 439-442.
16) Wannasin J, Janudom S, Rattanochaikul T, Canyook R, Burapa R,
Chucheep T, Thanabumrungkul S. Research and development of gas
induced semi-solid process for industrial applications. Transactions
of Nonferrous Metals Society of China, 20 (2010), pages s1010-s1015.
[Journal Impact Factor = 0.68]
17) Canyook R, Petsut S, Wisutmethangoon S, Flemings MC, Wannasin
J. Evolution of Microstructure in Semi-solid Slurries of Rheocast Aluminum Alloy. Transactions of Nonferrous Metals Society of China, 20
(2010), pages 1649-1655 [ISI Journal Impact Factor =0.751]
18) Rattanochaikul T, Janudom S, Memongkol N, and Wannasin J.
Development of an Aluminum Semi-Solid Extrusion Process. Journal
of Metals, Materials and Minerals, 20 (2010), pages 17-21.
19) Janudom S, Rattanochaikul T, Burapa R, Wisutmethangoon S,
Wannasin J. Feasibility of semi-solid die casting of ADC12 aluminum
alloy. Transactions of Nonferrous Metals Society of China, 20 (2010),
pages 1756-1762 [Journal Impact Factor = 0.44].
20) Rattanochaikul T, Janudom S, Memongkol N, Wannasin J. Development of aluminum rheo-extrusion process using semi-solid slurry at low
solid fraction. Transactions of Nonferrous Metals Society of China, 20
(2010), pages 1763-1768 [Journal Impact Factor = 0.44].
21) Burapa R, Janudom S, Chucheep T, Canyook R, Wannasin J. Effects
of primary phase morphology on mechanical properties of Al-Si-Mg-Fe
alloy in semi-solid slurry casting process. Transactions of Nonferrous
Metals Society of China, 20 (2010), pages s857-s861 [Journal Impact
Factor = 0.44].
22) Chucheep T, Burapa R, Janudom S, Wisuthmethangoon S, Wannasin J. Semi-solid gravity sand casting using gas induced semi-solid
process. Transactions of Nonferrous Metals Society of China, 20 (2010),
pages s981-s987 [Journal Impact Factor = 0.44].
23) Thanabumrungkul S, Janudom S, Burapa R, Dulyapraphant P, Wannasin J. Industrial development of gas induced semi-solid process.
Transactions of Nonferrous Metals Society of China, 20 (2010), pages
s1016-s1021 [Journal Impact Factor = 0.44].
24) Wannasin J, Thanabumrungkul S. Development of a semi-solid
metal processing technique for aluminium casting applications. Songklanakarin Journal of Science and Technology, 30 (2008), pages 215-220.
25) Wannasin J, Janudom S, Rattanochaikul T, Flemings MC. Development of the Gas Induced Semi-Solid Metal Process for Aluminum
Die Casting Applications. Solid State Phenomena, 141-143 (2008),
pages 97-102.
26) Wannasin J, Canyook R, Burapa R, Sikong L, Flemings MC. Evaluation
of Solid Fraction in a Rheocast Aluminum Die Casting Alloy by a Rapid
Quenching Method. Scripta Materialia, 59 (2008), pages 1091-1094 [ISI
Journal Impact Factor =2.699].
27) Kuntongkum S, Wisutmethangoon S, Plookphol T, Wannasin J.
Influence of heat treatment processing parameters on the hardness
and the microstructure of semi-solid aluminum alloy a356. Journal
of Metals, Materials, and Minerals, 18 (2008), pages 93-97.
28) Wannasin J, Martinez RA, Flemings MC. Grain refinement of an aluminum alloy by introducing gas bubbles during solidification. Scripta
Materialia, 55 (2006), pages 115-118 [Journal Impact Factor = 2.22].
29) Wannasin J, Martinez RA, Flemings MC. A Novel Technique to
Produce Metal Slurries for Semi-Solid Metal Processing. Solid State
Phenomena, 116-117 (2006), pages 366-369 [Journal Impact Factor
= 0.49].
30) Wannasin J, Schwam D, Yurko JA, Rohloff C, Woycik GG. Hot
Tearing Susceptibility and Fluidity of Semi-Solid Gravity Cast Al-Cu
Alloy. Solid State Phenomena, 116-117 (2006), pages 76-79 [Journal
Impact Factor = 0.49].

32





1)
2552
2)

2550
3)
2553
4)
. 2551
5)
2550
6)

2551

7)


6 2551
(PEC-6)

8)
Thainox Metallurgy Award 2006
Development of a Novel Semi-Solid Metal Processing
Technique for Aluminium Casting Applications.
()
13 .. 2549
9)
Thainox Metallurgy Award 2006
Semi-Solid Gravity Casting of an AluminumCopper Alloy
() 13 .. 2549
10)
Thainox Metallurgy Award 2007
A Study of Semi-Solid Die Casting by Gas
Induced Semi-Solid Process
() 15 .. 2550

1. 1 6 Plenary Speakers 12th International Conference on Semi-solid Processing of Alloys and Composites Cape Town South Africa
8 11 October 2012
2. Evaluation of Solid Fraction in a Rheocast Aluminum Die Casting Alloy by a Rapid Quenching
Method. Science Direct Top 25 Hottest Article in Scripta Materialia OctoberDecember 2008
3. Grain Refinement of an Aluminum Alloy by Introducing Gas Bubbles during Solidification
Science Direct Top 25 Hottest Article in Scripta Materialia April-June 2006

33

SensibleTAB

Prakarnkiat Youngkong, PhD.

Institute of Field Robotics (FIBO)
King Mongkuts University of Technology Thonburi

SensibleTAB

X-Y 2



Passive,
Assistive Resistive

3




Real-time

Abstract

SensibleTAB, a novel robotic arm trainer,

was designed, developed and constructed for arm
rehabilitation in patients who suffer from the effects of
brain injury or stroke. Basic knowledge of robotics and
automation, including mechanical design that applies
2DOF X-Y table which could be tilted as desired by
operators, electrical circuit that is clinically designed
and advanced control system was intensively applied in
order that high repetition, task specific and impairment
appropriate passive, assistive and resistive training could
be effectively provided for almost every severity of
complication. A super sensitive 3DOF sensor system
was designed and attached to measure how much force
these patients apply during the therapy. Furthermore,
this novel robot could create virtual environments, for
instance zero gravity and frictionless, and virtual objects,
for instance spring, damper and force field, which are
real-time monitored and controlled via a graphic user
interface of a central processing unit.
34

Cognitive Sensory Motor Training Therapy

35

X-Y
2
X-Y

Passive, Assistive Resistive

(Technology content)

SensibleTAB
100% 1)


2)
Short
wave 3)
10



4)
Real-time

....................................................................

(Technology
readiness level)

Concept Formulation,
Lab Demonstration, Engineering Prototype
Full Operation

.
SensibleTAB
Full Operation


1,000

36

.............................................................................................

.........

(Impact)

(High repetition)

(Task specific)
(Sensory training)

Cochrane Collaboration






SensibleTab

..2555
37

1.
SensibleTAB
1201000340
2.
Design Innovation Contest
2012
3. 2014 Engineering Impact Awards ASEAN
Regional Contest Best Innovation in
Biomedical and Pharmaceutical Applications
National Instruments
4. J.Chuanasa, P.Wongphaet and P.Youngkong
SensibleTAB Robotic Device for Hemiplegic
Shoulder and Arm Function Rehabilitation:
Preliminary Clinical Results. 8th International
Convention on Rehabilitation Engineering and
Assistive Technology 2014, Singapore, August
2014.
5.
a. (ThaiPBS)
b. (Nation)
c. 9Sci ( 9 )
d.
( 3)
6.
a .
SensibleTAB (InnoMag)
b. Eure!ka ()

positive-sense RNA

Bunpote Siridechadilok, Ph.D.

National Center for Genetic Engineering
and Biotechnology (BIOTEC)
National Science and Technology
Development Agency (NSTDA)

.




(Brown University, Rhode Island, U.S.A.)

(University of California, Berkeley,
U.S.A.)

molecular virology

38

Abstract

Recombinant DNA technology is an indispensable tool in

virology and the development of viral vaccine and antiviral drugs.
Conventionally, a virus is genetically modified by cloning techniques
that necessitate the use of the bacteria Escherichia coli to propagate
the DNA form of viral genetic material. However, many of the viral
DNA clones are not stable in E. coli, and often require months of
construction effort. We discovered a powerful virus construction
that greatly cut down the effort and enables the application of
diverse genetic approaches that could not be effectively performed
with conventional cloning methods. We found that multiple DNA
fragments of dengue viruses could be efficiently assembled by
Gibson-assembly technique and then directly introduced into
host cells to generate dengue viruses, negating the need to use
E. coli. With this method, a recombinant dengue virus could be
produced and verified by DNA sequencing within two weeks. We
have applied the technique to quickly exchange the genes between
two dengue viruses to map the mutations that confer a specific virus
phenotype (forward genetics). This technique allows us to quickly
construct a collection of mutant dengue viruses (virus library) with
all possible genetic variations at an amino-acid position critical for
viral growth. The virus library could be screened and analysed by
a high-throughput method. This approach of virus construction is
now being used to develop new dengue vaccine strains, to identify
potential drug-resistant mutants, and to study the interaction of
dengue virus and host immune components.

39

(recombinant DNA technology)

mutations mutations

DNA infectious-clone plasmid (infectious-clone

plasmid DNA
) infectious-clone plasmid
infectious-clone plasmid
Escherichia coli cloning host
E. coli



(BIOTEC)
DNA
genome (
seamless DNA assembly) Gibson Assembly
DNA
E. coli infectious-clone plasmid
Gibson Assembly
DNA DNA DNA viral RNA (
)
DNA 11 ( DNA
) E. coli
infectious-clone plasmid
(high-throughput analysis)

40

64 3-4 4

DNA

forward genetics
reverse genetics
reverse genetics
reverse genetics



forward genetics

DNA (DNA synthesis) DNA

41

( Grand Challenges Canada

)

(BIOTEC)
(.)
BIOTEC

BIOTEC Imperial College
. (BIOTEC) . (
) .
BIOTEC BIOTEC Young Fellow Research Grant
Award Platform technology
Journal of
Virology 2013 (Siridechadilok B, Gomutsukhavadee M, Sawaengpol
T, Sangiambut S, Puttikhunt C, Chin-inmanu K, Suriyaphol P, Malasit P,
Screaton G, and Mongkolsapaya J. (2013) A simplified positive-sense-RNA
virus construction approach that enhances analysis throughput. Journal
of Virology 87:12667-12674)
seamless DNA assembly
Journal of Virology Prof. Alexander
Khromykh University of Queensland, Australia CPEC
West-nile virus Daniel G. Gibson (
Gibson assembly) J.Craig Venter Institute, U.S.A. Novartis
Science Translational Medicine Gibson Assembly
DNA

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