You are on page 1of 2

Topic 7: Nucleic Acids Questions

Chapter 7.1: DNA Structure & Replication
11. DBQ: The Hershey-Chase Experiment (pg. 344-345)
a) Supernatant: The soluble liquid fraction of a sample after centrifugation or precipitation
of insoluble solids.
http://www.biology-online.org/dictionary/Supernatant
b) It should be found in the pellet since it is much heavier in weight than the virus. Thus, the
virus will be found in the supernatant since it is much lighter, and is able to float on top
of the solution.
c) 80%
d) Since most of the genetic material is left in the pellet, it is able to be injected inside the
bacteria cells. Thus, transforming the bacteria into infected cells.
12. Activity: Determining packing ratio (pg. 348)
a) 10:5
b) 1:10 000
13. DBQ: Apoptosis and the length of DNA between nucleosomes (pg. 349)
1. i. 250 bp
ii. 500 bp
iii 750 bp
2. 250bp
3. The fragments might become more defined and sharp like the right side, where the
fragments are clearly seen
14. Activity: Analysis of DNA profile (pg. 352-353)
Fragment size (bp)
Column 2

Distance moved
(mm)
Column 2
60
70
130

Fragment size (bp)
Column 3

15. DBQ: Analysis of DNA profiles using D1S80 (pg. 353)
a) 123, 246, 369, 492, 615, 738, 861
b) G
Distance moved (mm)
0.35
0.80
1.30
1.80

Fragment size

Distance moved
(mm)
Column 3
70
160
200

8 700 500 1.1 1.2 3.5 500 200 3 1.8 600 500 4 5 6 d) .25 4.1 600 250 1.20 c) Individual 1 Distance moved (mm) 2.50 3.7 Estimated fragment size 400 350 2 1.4 2.8 600 300 1.7 3.6 1.2.4 2.