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How to Contain the Zombies and

Combat an Outbreak

By: The Zombie Busters


(Morgan Gray, Megan Carpenter, Maegan Sours, Quinn Noonan)

The Problem
An outbreak has occurred at Monarch University in
Louisville, Colorado.
Several students seem to have "zombie-like"
symptoms and four have died as a result.
Several exposed students are on the prowl.
The University President, Mrs. Doodles Donley, has
called the Center for Disease Control and
Prevention and Homeland Security to send in
teams to investigate the outbreak and contain it.

The Problem
So far, the students don't realize they are sick. They think they are normal and possibly
have been exposed to something in the college bowling alley ice cream that has
made them into "super soldiers," so it is hard to get them to come in to be
quarantined. They do say that everyone else is "acting strange" and moving and
talking really, really fast.

How to Contain the Zombies and


Combat an Outbreak

Background Information

Bacteria 101
Shape:
Bacteria can be found in five different shapes
that signify what type of bacteria it is. RodShaped (Baccilus), Spherical (coccus), Curved
(Bibrio, spirochaete), Square-shaped (Arcula)
and Star-shaped (Stella).

Arrangement:
Bacteria is also arranged in either a pair
(escherichia), chain (streptococcus) or a
cluster (staphylococcus).

Bacteria 101
Basic Makeup of a Bacterium:
Bacteria contains a cell wall to protect cells against osmotic shock, a membrane to
regulate transportation in and out of the cells. A chromosome is the genome while
the Plasmid contains genetic information like resistances to antibiotics and
tolerance of toxins. Ribosomes take part in protein synthesis. Flagella helps the
cells move. Body is the storage of cells. Pili is how the bacteria mate. Endospore
is heat resistant and helps the bacteria survive. (
http://www.fastbleep.com/biology-notes/35/112/654)

Growing bacteria is as easy as it sounds. You simply need a product and if you
keep it in a incubator, soon enough bacteria will grow.

Bacteria 101

https://www.youtube.com/watch?v=8V2VpeJdJDc

How to Contain the Zombies and


Combat an Outbreak

Purpose/ Methods

Abstract
We are testing the effectiveness of different natural antibiotics to disperse the
E.Coli bacteria/virus that is making the people zombified. Our antibiotics are
coconut oil, tea tree oil, clove, cinnamon, oregano, and honey. We tested all of
these with the hope that a couple would be able to serve as an antibiotic for the
virus. This is important because we need to know what natural resources we can
use in order to control the Zombie Apocalypse. The Zombie Apocalypse is getting
out of hand and if we dont test these natural antibiotics, then it may be too late to
control the zombies.

Methods- Materials
Sterile loop
E.Coli bacteria
2 Agar dishes
Parafilm
Paper cutouts for soaking the natural antibiotic in
teaspoon of tea tree oil, clove, cinnamon, oregano, honey, coconut oil
Sanitation supplies (wipes and cleaning spray)

Methods- Procedure
1. Obtain an agar disk that has either E.coli or yogurt
bacteria on it
2. Create solutions with 3 pipettes of distilled water and 2
parts natural antibiotic
3. Soak 5 different paper disks in various solutions for at
least 10 minutes
4. Remove the paper disk from its solution and place on
agar dish
a. Do not let the paper disk drip onto the agar plate
5. Place the plate in the incubator and let the bacteria grow
for at least 24 hours
6. Remove the plate from the incubator and observe and
record the field on inhibition

Methods- Procedure (How to plate bacteria)

https://www.youtube.com/watch?v=Ukdb5VjntSM

How to Contain the Zombies and


Combat an Outbreak

Results

Conclusion- Results
What happened?
In our experiment to try and find a cure, we unfortunately concluded no natural
antibiotics that work, but our scientists are hard at work to find a cure.

Conclusion- Results (Data Table)

Conclusion- Results
Plate 1: Coconut oilthis area on the plate shows a very
large expansion of the bacteria around the whole paper
because the coconut did not work at all. If anything it grew
more of the bacteria, almost as much as the control group.
Plate 2: Clovethe clove was the only natural antibiotic we
tested that had any significant change to the growing
bacteria. There was about a 1mm inhibition size, which was
remotely small comparatively to another stronger antibiotic
that we couldve used.
Plate 3: Tea Treethis area on the plate didnt grow as
many bacteria around the paper sample, but it didnt make
the bacteria disperse either. There werent too many
bacteria only a thin layer, about half of the control group.

Conclusion- Results
Plate 4: Oreganothere was a thick area of bacteria
around the paper sample for oregano. It was also tinted
orange, which may say that it was contaminated.
Nonetheless bacteria grew and were not dispersed.
Plate 5: Cinnamonmany bacteria grew in this area,
closely related to the control group of no natural antibiotic.
The paper for this antibiotic had large coverage of the
bacteria.
Plate 6: Honeylarge bacterium can be seen around the
honey which concludes it did not work very well. This
specific area is worse than the control on the same plate
with a thick white layer of bacteria around the paper
sample.

Conclusion- Results (Observed)

Conclusion- Results (Observed)


Unfortunately the only natural antibiotic
that worked was clove. It still have a very
small zone of inhibition (about 1mm),
which could have been due to improper
plating
If we were to repeat this experiment we
would have soaked the paper disks in
more concentrated solutions.

Methods- Results (Predicted)


This picture shows clear zones of
inhibition, unlike our results. These
results show that the antibiotics
used in this test killed the
surrounding bacteria. In our test
we hoped that the natural
antibiotics would have killed the E.
Coli bacteria.

Methods- Results

E.Coli is gram positive according to our results. The gram positive bacteria means that it has a
thick cell wall.

How to Contain the Zombies and


Combat an Outbreak

Conclusion

Conclusion- Results
Margin of error
Our margin for error could have been prevented
through using more precautions when we plated our
bacteria and when we would open and close the
plates.
When finding a cure for the antibiotics, we could have
used a more concentrated solution of the disks on
the plate so that they were more efficient and
effective at killing and stopping the disease from
spreading.

Conclusion- Results
Whats next?
In our future research, we are going to
keep trying to find as cure as that is the
most important step for containing the
outbreak at Monarch University.
We are going to develop a vaccination
that can combat against the disease
and prevent it from happening in the
future. With further research we want to
develop antibodies that will be injected
into the bloodstream so that the disease
can subside more quickly.

How to Contain the Zombies and


Combat an Outbreak

Zombie Apocalypse
Containment

Zombie Apocalypse Containment


How is this disease Spread?
Students fell sick after eating the ice cream at the bowling alley, so it might be due to
food contamination.
Food-borne illness information
It is not known if the disease has the capability to evolve other modes of infection, so it is
important to take all precautionary measure to ensure public safety.

Zombie Apocalypse Containment


What are the symptoms?
Hard to tell if you are sick, but if you
feel like you have gained
superpowers or if other around you
start to act strange and talk fast,
you must go find a doctor as soon
as possible
Trust family members and close
friends, if they believe that you are
sick take a precautionary measure
and go to the doctor to be
evaluated.

Zombie Apocalypse Containment


How to quarantine:
The CDC defines two steps in order to reduce the spread of disease
Isolation separates sick people with a contagious disease from people who are
not sick.
Quarantine separates and restricts the movement of people who were exposed
to a contagious disease to see if they become sick.
The general public should avoid the bowling alley ice cream, until researchers have a
chance to thoroughly investigate the source.
Avoid contact with those who may have been infected and if you experience
symptoms, admit yourself to a hospital and avoid contact with others

Zombie Apocalypse Containment


CDC Controlling outbreaks

How to Contain the Zombies and


Combat an Outbreak

Bibliography

Bibliography
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http://www.jove.com/video/3064/aseptic-laboratory-techniques-plating-methods
MicrobeWorld. (n.d.). Retrieved October 24, 2015, from http://www.microbeworld.org/types-of-microbes/bacteria
Global Public Health Threats. (n.d.). Retrieved October 24, 2015, from
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Bacteria. (n.d.). Retrieved October 24, 2015, from
http://www.microbiologyonline.org.uk/about-microbiology/introducing-microbes/bacteria

Photo Credits
http://www.aliexpress.com/store/product/ZOMBIE-OUTBREAK-RESPONSE-TEAM-BIOHAZARD-SYMBOL-VINYL-DECAL-CAR-WINDOW-STICKE
R/126209_1957583335.html
http://www.amazon.com/LAMINATED-Zombie-Research-Facility-Poster/dp/B00A809X6Y
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http://blog.heartland.org/2014/01/antibiotic-surprise/
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