J: Biotechnolegy

Q.l- Q .. " carry one mark each.

Q.I A. thermostable DNA polymerase that can carry out both. reverse transcription reaction and polymerization has been isolated from

{A) Tliermococcus litoroiis. {C) Theml()logp trllltilimr1

(B) Thermus aquai(CiiS (D) Thermw; themuJlpllillls

Q,2 When presena ill tissue culture medium, gibberellin

{A} helps to break dormancy of buds and bulbs

(B) pl'OI1l!otes dormancy development 111 buds and bulbs (C) is regarded as plant growth inhibitor

(D) preventsnormal recognition of auxin molecule

Q.:l To promote: attachment and spreading of ancherage-depcndent animal cells, the surface of the culture vessel needs. to be coated with

(A} trypsin

(B) collagen

(C) pronase

(D) polyglycol

Q.4 For ampli ficauon of GC rich sequences by polymerase chain reaction, identify the reagentrhai binds and stabilizes AT sequences and destabi lizes GC regions.

(A) Terrametbyl ammonium chloride (Bl Betaine

(C) 7 -deaza-z' -deoxyguanosiae triphosph.il.le (D) Sodium dodecyl sulphate

Q.5 Which oftne following statements IS INCORRECfabol.l1 immobilized plant cell cultures?

(A) his pnssible 10 lise high eel! densities (8) Cells remain active for long periods

(C) Cell products or j nhibitors can be removed easi Iy (0) f'I provides lowshear resistance 10 cells

Q.6 A III tbecells 111m participate ill i InmLIOC responses originate from a population or

(A) neutropbils

(B) stem cells

(C) macrophages

(0) lymphocytes

Q. 7 - Q. 24 ca:rry two marks each.

Q.7 ldentif'y the natural plant growth regul ators from the fo II owing .1 iSL (P) Zeatin

(Q Benzylaminopurine (BAP) (R) Indole-S-acetic acid (lAA)

(S) 2.4-DicI11orophenoxyacetic acid

(A) p, Q

(B) Q, S

(C) P,. R.

(D) R.S

Q.8 A hybrid derived from the fusion of a myeloma cell (HPRT -) with an antibody secreting Bslymphocyre (HPRT +) can be selected LO produce monoclonal antibody by growing ill a medium containing

(A) thiamine, hypoxanthine, aminopterin (B) thymidme, hi tidine, aminopterin

(C) uridine, hypoxanahine. aminopterin (D) thymidine. hypoxanthine .. aminopterin

Q.9 Match items in group' L with correct options from those given in group 2 ..

GrolLlP 1

P. VNTR sequence

Q. Leader sequence

R. SD sequence

S. cis-acting sequence

(A) P-3. Q-I .. R-4 .. S-2 (C) P-3. Q-4. R-2. S-I

Group 2

1" Gene regulation on the' same chromesome . Ribosome binding site

3. D A finger printing

4. Functions in attenuation

(II) P.·2o Q-3. R-l. S-4 (D) P-3. Q; 1. ~{2, S-4

Q.lO During cultivation of microorganisms in a fermenter, various, parameters are controlled byappropriate sensor {probe}. arch each probe in group 1 with ihe appropriate response mechanism in group 2.

Group 1 {Probe) P. hermisror

Q. Oxygen electrode R Metal rod

S. pH electrode

(A) P-2, Q-3 R-l. S-4- (C) P-3. Q·2, R-4. S·.I

Group' 2 (Response)

L Activation of acid I alkali pump

2. Activation of vegetable oil pump

3. Acii vation of hot I cold water pump

4. Increase I decrease in stirrer motor speed

B) Pol Q-? R;4 S- ':l

.• "'. _'~ '11' oJ

(D) P-3. Q-4, R-2; s-i

QJ 1 Which of these mice fail to develop a thymus?

(A) Mountain mice (B) Beige mice

(C) Knock out mice (0) Nude mice

Q.J2 'What are the experimental reps needed for screening an expression library for a clone encoding a protein X thai has been isolated and purified?

(P) m-RNA isolation

(Q) Antibody preparation

(R) Cloning into an appropriate vector

(S) Western blotting on transferred plaques

{A) P, S

(B) Q, S

(C) Q,R

CD) R, S

Q.13 When electroporation is used for introducing D A into mammalian cells (P) a earlier for DNA is not required

(Q) tile lipid bilayer (membrane) interacts with an electric pulse to generate permeation shes

(R) the viability of the cells becomes approximately zero percent (5) the first step involve' absorption of DNA On the cell membrane

(A) p.Q

CB) Q, R

(C) P, S

(D) Q, S

Q.14 Immobilization of enzymes using entrapment method requires (P) photosensitive polyethylene glycol dimethacrylate

(Q) CNBr activation of sepharose

(R) polyfunctional reagent like hexamethylene diisocyanare (S} radiation of polyvinyl. alcohol

(A)P.Q

(B) R, S

(C) p, S

(0) Q. S

Q.15 Which one of the following monolayer culture systems have the highest surface area : medium ratio?

I A ' Roux bottle (C) Hollow fibres

(B) Spiracellroller bottle (D) Plastic bag/film

Q.16 Majority of the cereals are highly recalcitrant to AgtobaCierium-mediated transformation. and so direct transformation methods have been developed to transform such plams, Which of the following direct transformation methods is applicable La inract plant tissues?

(A) Calcium chloride and PEG-mediared transformation (B Liposorne-rnediated transformation

(C ' Electroporation

10, Transformation using rnicroprojeetiles

Q.! 7 Match items in group 1 with correct options from those given in group 2

G:rou p ] GJlOliP 2

P, Tissue plasminogen activator 1. Irnruunomodulator

Q. Gamma interferon 2. Biodegradable plastic

R. Podophylloroxin 3. CLot disselutinn

S. Polyhydroxyalkanoate 4. Anti-tumor agent

(A) P-4, Q-3. R-l, S-2 (C) P-3, Q-l, R-2, 8-4

(B) P-l. Q-3. R·4, S-2 (D) P-3,Q-I, R-4, S-2

Q.18 Match Hems in group 1 with correct option from those given in group 2

Group 1

P. Amperernetric biosensor

Q. iEvanesoentwave biosensor lR. Calorimetric biosensor

S. Potentiometric biosensor

GrOUIJ 2

1. Light beam

2. PI UX. of redox electrons

3. Field effect rrunsisrors

4. Exothermic reaction

(A) P-3;. Q-4, R-2, s-i (q P-3, Q-2. R-4, 8-J

(B) P-2" Q-J., R-4, $-3 (D) P-2; Q-4; R-3, S-I

Q,19 Forprediction of three dimensional structure of protein (P) homology modeling tries many possible alignments (Q) threading first identlfles homolegues

,(R) threading evaluates nlmiy rough models

(S) homology modeling optimizes one model

(A) Q, S

fB) P, Q

(C) R, S

CD Q.R

Q.20 Immobilization of enzymes

(P) increases the specificity of the enzyme for its reactants (Q) facilitates reuse of the enzyme in batch reactions

(R) makes it unsuitable for its use ill a continuous reactor system (S) decreases the operational cost of the industrial process

(A) Q, S

(B) Q, R

(C) R. S

(0) P. Q

Q.21 Which of rhe following would result in somaclonal variation in mieropropagated plants?

(P) Propagation by axillary branching iII the absence of plant growth regulators (Q) Cell suspension maintained for five years before induction of somatic:

embryogenesis

(R) Callus induction using 20~!M 2A-Dichlorophenoxyacetic acid followed by ShOOl organogenesis

(S) Shoot organogenesis from an explant in 'the absence of an Intermediate callus phase

(A) P, Q

(B) Q, R

(C) P,S

(D) Q, S

Q.22 The enzymes that can be used in S'ClIG labeling of DNA are (P) alkaline phosphatase

(Q) DNA ligase

CR) terminal. transferase (3) polynucleotide kinase

(A) P, S

(B) R. Q

(C) P. R

(D) R. S

Common Data Questions Common nata. for Ques.tions23, 24:

Lignocellulosic biomass was. subjected to microbial composting .. The microbial .CQIlSQTtil1m produced an extracellular enzyme xylanase, which was a glycoprotein having a molecular weight of 68 kDa and a positive charge. An aqueous extract of the enzyme could be easily prepared from the compost -

'0.23 What techniques would you recommend for confirming the molecular weight of the purified enzyme?

(1:') Isoelecrric focusing (Q) SDS-PAGE

(R) Native PAGE

(S) Gel filtration

(A) P, Q

(B) Q, S

(C) R, S

(D) P,S

Q.24 If Con A sepharosecolumn was used for the purification of enzyme, the separation would be based on

(A} molecular exclusion (C) ion exchange

(18) affinity binding

(D) hydrophobic interaction

LiDked Answer Question: Q. 25 to Q. 26 carry rwo marks each,

Sta.t~m.ent for Lli~.ed Anslll'el' Question. 25 & 2,6:

DNA content of CaenorJwbditis elegans was analysed and found to contain 1.0:x. ~ 08 bp.

Q.25 HO:\i' many standard A- phage vector carrying 20kb DNA fragments or YACs carrying 250kb DNA fragments are theoretically required to eenstitute a. eomplete c: elegan genomic library?

(A} SOO A- phage vectors 'Of 40 yeast clone's

(B) 400it~ phage vectors 01 5000 yeast clones (C) 50001- phage vectors or 400 yeast clones (0), 5 lit 1 O'll~ phage vectors or 4000 yeast clones

Q.26 HO\iV many A- phage vectors f yeast clones should be prepared in order to ensure that every sequence is included ill the library?

(A} 25 x [03 "A- phage vectors /2000 yeast clones

(B) 20 x lo3l- p,hage vectors /1600 yeast clones

(C) 5 x 104. J..,. p]lage vectors f 4000 yeast: clones

(0), 10 x [04 J",- phage vectors. f 10000 yeast clones

<t3l,elileHt roo' UrI,lred AI~S\H!:r Q'.nelilijctl1l:~~'7 ,S: 16;

A blol't~~\o;r 01' i,.\'0rking volu me 5Q In - rll{)il:ltJ ,'c:'s a I']i):.':~ ~1iJ0ihe (X in batch C11ILuff under ,gi""ii:Ji 9rel<llil~1 L:I)I1(liliQIl~ lrom ill ~J~S']Jitll L5). 1I1e fi(:jal"o.,;Q}I~iur~tlo.l1 e-'i met~lb<i]lil~ X)'H, tilt. end of ,rgar,:'1! rUfli \\ ~s U l:,& n'-', Tile ~i(!rearmr' w~s operated it'! C(I'!11plele 10 wI'!:> in ~tJ:cllj'ear,

Q,17 Wh,l!t w~ I be I;hC:'l<Eilfllm:ll1 (I~HVIti~ Olf the system ~ [:i1'OO1Itl ion of J'I:i:e&IIl;.oli~t!' eX)) i,.. kg ~r

~rt '

(A) 55

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