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Gate 2006 Microbiology

Gate 2006 Microbiology

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L: Microbiology

Q. 1 - Q. 6 carry one mark each.

Q.l Which of the following scientists developed the modem concept of chemotherapy and chemotherapeutic agents?

(A) Robert Koch (B} Paul Ehrlich (C, Joseph Lister (D) Louis Pasteur

Q.2 The refractive index of the immersion oil used in microscopy to achieve higher resolution is

(A) Same as glass (B) Less than air (C) Less than glass (I)) Same as air

Q.3 Which of rhe following synthetic antibacterial compound inhibit supereoiling of bacterial DN A?

{A) Sulfonamide

(8) p-Fluerophenyl alanine' (C) Puromycin

(0) Rifampin

QA Which one of the following is nOI a lymphocyte?

(A) B-cell (8) T-cell (C) NK-celi (D) Mast-cell

Q.5 Which of the following rganism has a si ngle stranded positive sense R A genome?

(A) Influenzavirus (B. Poliovirus

(C) Hepatitis B virus (D) Pox vi rus

0.6 Cyanobacteria comprises a larze and morphologically heterogenous group or

(A) Chemoautotrophs (B) Photcheterotrophs

( ) Phoroaurorrophs (D) Chemoheterotrophs

Q. 7 - Q. 24 carry two marks each.

Q.7 111 the peptidoglycan layer of bacterial eel] wall. which of [he following pail' of amlnoaclds are usually round ill D-conflguration?

(." Alanine and glutamic acid (8) Alanine and Jy ine

(C) Alanine and arginine

(I)) Glutamic acid a.nd lysine

Q.8 Which or the lollowing inclusion bodies contains the enzymes responsible for carbon dioxide fixation in bacteria?

(A) Lysosomes (B) Peroxisomes

~C) Metachromatic granules (D) Carboxy om s

Q.9 When bacterial cells are placed in <1 2 M ':iCI solution, the plasmaraembrane will

(A) Burst

(B) Undergo plasmolysis (C) Remain unchanged CD) Swell

Q.I.O Which of the Following couple will have the maximum tendency to donate electrons? (Redox potentials. are given in the parenthesis)

2"H I H2 (-0.4_

(B) NAD+ I NADH {-(U2Vj (C) N03-1 NOz- (+O.42V) (DI Y.!02IH2.0 (+O.82V)

Q.II Match [he following group of microorganism with their oxygen requirements.

GI"'OUll of microorganisms Oxygen. reqlliirement
P. Obligate aerobe L Grows equally well in presence or absence of
I OXYP'en
Q. Microaerophile 12. Growsonly ill presence of OXYI;'<m
R. Obligate anaerobe 3. Cannot tolerate oxygen
S, Aerotolerarn anaerobe 4. Can grow only at reduced oxygen levels (A) P-J: Q-1: R·2; S-4 (B) PA; Q-3: R-2: S-J (CJ P':~: Q-4; R-3; S-I (D) P- L: '0-4: R-3; S-2

Q.I.2 In which of rhe following cases of microbial growth. lag phase usually does not occur?

(A) If inoculum is taken from old (stationary phase) culture and inoculated into same medium

(B) Irtoculum consists of damaged cell s (but not ki lied). inoculated into the same medium

(C) Inoculum is transferred from a dell culture medium to a poorer one

(D) If an exponentially growing culture is inoculated into the same medium under the same conditions of growth

Q.l3 Which one of The following statements is INCORRECf about bacterial endospore?

( » Core pHis about. 5.5 to 6.0 (B) Resistant to lysozyme

(C) Dipicolinic acid is present

(D) Small acid soluble protein is absent

Q.14 The following reaction of glyoxylare cycle requires two enzymes P and Q

Isociuare

p

succinate 4- glyoxylaie

Glyoxylate + acetyl CoA

---"Q._' --Jio .. malate + CoA

Which of the following combinations is the true representative of P and Q?

(A) Isocitrate lyase and malate synthase

(8) Isocitrate lyase and malate dehydrogenase

(C) Isocitrate dehydroxygenase and. malate dehydrogenase (D) Isocitrate dehydrogenase and malate synthase

Q.]) Which of the following statements is NOT included in Koch's postulate?

(A) A speci I·ic organism can always be found in ussociatlon with a given disease (B The organism can be isolated and! grown ill pure cultures in the laboratory (C) The pure culture will produce the disease when inoculated into susceptible

animal

CD) It is possible to clone the genome of the organism from the experirnentally infected animal

Q.16 The commercially used technique for pasteurization of milk involves low temperature holding (LTH) and high temperature short time (HTST) methods. Which of the following methods is INCORRECT?

(A) expose milk (0 14S"F for 30 min (B) expose rni lk to 16 (F for 15 sec (C) expose milk to 143" F for 30 min (0) expose ~nilk to 71.7" C for L5 sec

Q.I.7 The phenomenon in which a prophage 'is able to make .hange in the properties of a hosi bacterium in lysogeny IS termed as

(AI Immunity repression

I B I Lysogenic induction Ie) ysogenic conversion (D I' Lytic infection

Q.18 Phage typing is frequently used in medical diagnosis for the identification or certain strains of pathogens, such as

I A) Srapbylococci IB Enteroviruses

(C) Plasmodium [aiciparuut (D) Leishmania donovan!

Q. L9 Which of the following virus needs <I helper virus Ior their genome replication?

(A) Hepatius A (B) Hepatitis D (C) Hepatiris C (D) Hepatitis E

Q.20 Which of the following viruses usually causes '!fll nt infection' in the human neuronal cells:

(A j Poliovirus

I B ~ Llpanese En ephalitis virus ,C J Herpes sim plex V] rus type I ~ DI Rabie,., \'Ul"LJS

Q.21 Match the correct combination ofioxin and the mode of action

Toxin Mode of action
P, Pertussis toxin I. Prevents release of gl ycine by nerve end
Q. Diptheria toxin 2. Blocks G-pfotein signal transduction
R. Botulinum toxin 3. Induces fluid loss from intestinal cells
S. Tetanus toxin 4. Inhibits protein synthesis ill eukaryotes
5, Causes haernolysis
6. Blocks release of acetyleholin by nerve end (AJ (1-3. Q-::!. R-:i. -4 ~B) 1:)-1,Q~4, R-6, 5-J (e) p-s. Q-6. R-3, S-2 101 P-l.Q-3. R-2.S-5

Q.22 The following amibioucs affect the bacterial protein synthesis with their site 01 action. Which of the following combinations is correct"?

Antibletic Site of action
P. Streptomycin 1. Aminoacyl tRNA association with ribosome
Q. Tetracvclin 2. Transpeptidation
R. Erythromycin 3. Translocation
S. Chloramphenicol 4 .. Initiation of protein synthesis (A) P-?: Q-.; R-4; Sol (8) P·4; Q-I; R-3; S-2 (C) P-I ;Q-4: R·3;S< (0) P-4; Q-I :R-2; S-]

Common Data Questions

Common Data fOI' Questions 23. 24:

Besides the repression I derepression coutrol of tryptophan operon the second level or regulation is known as attenuation. The presence DC \WOo tryprcphun codons within the trpl:

ontrols this phenomenon. The presence of tr:plopllU!'l-ch<Irged IRN. irp cau es [he premature termination or transcription, yields (I 140 nucleotide long leader sequence transcript .. By sitedirected mutagenesis the lWO UGG Trp codon of the trpl. sequence were modified to COG • arginine codon f<IP').

Q.23 Which of the following ami 110 acid() would be able to restore the attenuation control of trp operon?

CA) Tryptophan alone

(C) Tryptophan 'or arginine

(B I Arginine alone

(DI Neither arginine nor tryptophan

Q.24 Deletion of part cf tile trpl: region Will reSIJ11 HI

fA) Increase in (he nne of ex pression of 'trp' s true rural genes

(C) No change In file rate of expression of 'lip' structural genes

I B J Decrease in the rate or expres ion of 'trp' structural genes

ID) Inhibition of the expression of all. the genes in the operon

Linked A~1L'Hv~r Questions: Q. 25 to Q. 28 carry two merks each.

Statement for Linked II. wer QLLestion~ 25 & 26:

The nucleic acid from u microorganism was isolated and [he base composition was determined to be as follows:

A",,35%, '1';;;;;15%. G=35%. C-15%

Q.25 What could he the physical nature 01" the nucleic acid?

(A) Double stranded circular DN, .8) Double stranded linear DNA ) Single stranded linear DNA (D) Single stranded RN A

Q.26 The optical density of the above nucleic acid was measured al260 nm wavelength at 37 Qe and 9:;°C. What possible changes could you expect in the optical den ity with the increase in temperature?

lA) Signiflcant increase (B) Only two fold increase (C) Significant decrease (D) No significant change

Statement for Linked Answer Question 27 & 28:

In a bacterial culture initial cell population is 1 x 1.03 cells. Thegeneration time or the bacterial cell is 20 minutes and the Jag phase is 1 hour.

0.27 IF the culture is allowed to grow for 'Ii hours, how many generations would take place?

(A) 8 (B) 12 (C) 10 (0) 9

Q.2:S What will. be the cell population after 3 how's?

6 (A) 6.4 x ]0

(B) 3.2 x:: 104

(Cl3_2 X .10'"

(0) 6.4 X W"

END OF THE SECTION

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