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Exercise 8: Chemical and Physical Processes of Digestion: Activity 2: Exploring Amylase Substrate Specificity Lab Report

Pre-lab Quiz Results
You scored 100% by answering 5 out of 5 questions correctly.
1. Which of the following is true of enzymes and substrates?
You correctly answered: c. Enzymes are specific about the substrates they can act upon.
2. Which of the following is/are reducing sugars?
You correctly answered: e. both glucose and maltose
3. Cellulose and starch are both
You correctly answered: e. polymers of glucose and polysaccharides.
4. Proteins and peptides are formed by joining amino acids with a special type of covalent bond called a peptide bond.
Which of the following enzymes do you think would be specific for a peptide bond?
You correctly answered: c. peptidase
5. The Benedict's assay tests for the presence of
You correctly answered: c. reducing sugar.

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Experiment Results
Predict Question:
Predict Question: Do you think test tube 3 will show a positive Benedict's test?
Your answer : b. no
Stop & Think Questions:
You included tube 2 to
You correctly answered: b. see what a positive Benedict's test should look like.
Which tube is included to detect contaminating amylase in the buffer or in cellulose?
You correctly answered: d. tube 4
What is the usual substrate for the pancreatic enzyme peptidase?
You correctly answered: d. peptides
Experiment Data:
Tube No.
1
2
3
4
5
6

Reagent 1
Amylase
Amylase
Amylase
Cellulose
Peptidase
Bacteria

Reagent 2
Starch
Glucose
Cellulose
Water
Starch
Cellulose

Reagent 3
pH 7.0
pH 7.0
pH 7.0
pH 7.0
pH 7.0
pH 7.0

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Time
60
60
60
60
60
60

Temp.
37
37
37
37
37
37

IKI
+
+
+
-

Benedict's
++
++
++

Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
1. The substrate(s) for amylase is/are
You correctly answered: a. starch.
2. The results of tube 5 demonstrated that
You correctly answered: b. peptidase does not digest starch.
3. Why was the cellulose in tube 6 hydrolyzed to glucose?
You correctly answered: d. The bacterial suspension contained the enzyme cellulase, which digested the cellulose.
4. Can you detect the presence of contaminating amylase from your experiments?
You correctly answered: c. It is not possible to determine whether contaminating amylase is present because amylase
doesn't digest cellulose.

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Review Sheet Results
1. Describe why the results in tube 1 and tube 2 are the same.
Your answer:
In tube #1 the amylase is hydrolyzing the starch to glucose and in tube #2 the glucose is already present in the hydrolyzed
form.
2. Describe the result in tube 3. How well did the results compare with your prediction?
Your answer:
The correct prediction is “no”. Tube #3 should not be positive for the Benedict’s test because amylase should not digest
cellulose.
3. Describe the usual substrate for peptidase.
Your answer:
. The usual substrate for peptidase is peptides and proteins.
4. Explain how bacteria can aid in digestion.
Your answer:
Bacteria can aid in digestion by breaking down cellulose which we do not produce cellulase.

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