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Exercise 8: Chemical and Physical Processes of Digestion: Activity 4: Assessing Lipase Digestion of Fat Lab Report

Pre-lab Quiz Results


You scored 75% by answering 3 out of 4 questions correctly.
1. Which of the following is/are true of bile?
Your answer : d. It works by a chemical and physical process.
Correct answer: b. It works by a physical process.
2. The substrate used in this simulation is
You correctly answered: c. vegetable oil.
3. When fatty acids are liberated by lipase, the pH
You correctly answered: b. decreases.
4. One of the products of the chemical digestion of lipids is
You correctly answered: d. fatty acids.

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Experiment Results
Predict Question:
Predict Question: Which tube do you think will have the highest lipase activity?
Your answer : d. tube 5
Stop & Think Questions:
What does the pH measure?
You correctly answered: d. both lipase activity and fatty acid release
Why is it difficult to detect whether lipase is active in tube 5?
You correctly answered: d. The pH is already very low, so a decrease in pH might be difficult to detect.
Experiment Data:
Tube No.
1
2
3
4
5
6

Reagent 1
Lipase
Lipase
Lipase
Water
Lipase
Lipase

Reagent 2
Vegetable oil
Vegetable oil
Water
Vegetable oil
Vegetable oil
Vegetable oil

Reagent 3
Bile salts
Water
Bile salts
Bile salts
Bile salts
Bile salts

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Reagent 4
pH 7.0
pH 7.0
pH 9.0
pH 7.0
pH 2.0
pH 9.0

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Time
60
60
60
60
60
60

Temp.
37
37
37
37
37
37

pH
6.21
6.72
9.00
7.00
2.00
8.97

Post-lab Quiz Results


You have not completed the Post-lab Quiz.

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Review Sheet Results


1. Explain why you can't fully test the lipase activity in tube 5.
Your answer:
Lipase activity in tube 5 is difficult to test because the reagent put into the tube is already very acidic (pH 2.0). That is why
after treatment and incubation, the resulting pH is still very acidic. It is difficult to determine if this is due to lipase activity or if
it is simply because of the acidic reagent.
2. Which tube had the highest lipase activity? How well did the results compare with your prediction? Discuss possible
reasons why it may or may not have matched.
Your answer:
My prediction and the results did not match. Highest lipase activity actually occured in the first tube wherein pH lowered the
most.
3. Explain why pancreatic lipase would be active in both the mouth and the pancreas.
Your answer:
Digestion is already happening in the mouth, which explains why lipase is active in the mouth. The same is true for the
pancreas.
4. Describe the process of bile emulsification of lipids and how it improves lipase activity.
Your answer:
Emulsification separates clumps of bile. This aids lipase activity.

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