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Vitamin Mixtures

Preparation of Solutions from Packaged Powder


Media Preparation using Vitamin Solutions
Vitamin Mixtures Composition Table
Preparation of Solutions from Packaged Powder Powdered vitamin mixtures are hygroscopic and must be protected from atmospheric m
entire contents of each package should be used immediately after opening. The basic steps for preparing 1000X concentrated solutions with
vitamin mixtures are listed below:

1.

Measure out 70% of the final required volume of deionized-distilled water (e.g. 70 ml for a final volume of 100 ml).

2.

While stirring the water add the powdered vitamin mixture. Stir until completely dissolved. Increasing the pH and/or w
solution (35-37 C) may be required.

3.

Rinse the original container with a small volume of water to remove traces of the powder. Add to the solution in Step

4.

Add additional water to bring the medium to the final volume.

5.

The resulting 1000X concentrated solution should be used at a concentration of 1 ml/L of medium.

6.

Follow the same steps to prepare a 100X concentrated solution and use at 10 ml/L of medium.

Media Preparation Using Vitamin Solutions

SIGMA Prepared Vitamin Solutions:


1.

The vitamin solutions are sterile filtered through a double 0.2 m filtration unit and are ready for use.

2.

Vitamin solutions should be added at a concentration of 1 ml/L of medium to prepare the final recommended concent

vitamins in the medium. (Except K 3129, Kao and Michayluk vitamin solution, which should be added at a concentrat
ml/L of medium.)

MATERIALS NOT PROVIDED

BASAL MEDIA AND ADDITIVES AS REQUIRED

Deionized tissue culture grade water (Product No. W 3500)

1 N Hydrochloric Acid (Product No. H 9892)

1 N Sodium Hydroxide (Product No. S 2770)

VITAMIN POWDERS AND SOLUTIONS ARE FOR LABORATORY USE ONLY. NOT FOR DRUG, HOUSEHOLD OR OTHER
STORAGE

Store powdered vitamin mixtures and solutions in a refrigerator at 0-5 C (except K 3129, Kao and Michayluk vitamin solution
should be stored at -0 C). Precipitation in vitamin solutions may occur during storage. This can be redissolved by warming th
in a water bath (35-37 C) for a short period of time.
Vitamin Mixtures Composition Table
G
1019
K
3129

Gamborg's vitamin solution (1000X)


Kao and Michayluk vitamin solution (100X)
Murashige and Skoog vitamin powder
Murashige and Skoog vitamin solution (1000X)

M
Schenk and Hildebrandt vitamin powder
7150
M
3900
S
3766
Component (mg/L)*

G 1019

K 3129

M 7150

p-Aminobenzoic acid

0.02

L-Ascorbic acid
Biotin

2.0
0.01

D-Calcium pantothenate
Choline chloride

1.0
1.0

Choline-H2 citrate
Folic acid

0.4

Folic acid, Ca
Glycine
myo-Inositol
Nicotinamide (Niacinamide)
Nicotinic acid
Orotic acid
Putrescine 2HCl
Pyridoxine HCl

100.0
1.0

1.0

Pyridoxamine 2HCl
Riboflavin
Thiamine HCl
Thymine

100.0
1.0

1.0

M 3900

S3

2.0

2.0

100.0

100.0

0.5

0.5

0.5

0.5

0.1

0.1

0.2
10.0

1.0

Vitamin A (Retinol)
Vitamin B12

0.01
0.02

Vitamin D (Cholecalciferol)

0.01

*Concentrations listed are final concentrations when used at recommended dilution