Professional Documents
Culture Documents
1 History,Taxonomy,
Morphology of Bacteria 13.6.2016
ASM Discussion on FB Round-2 (13.6.2016) 13.6.2016
#Chapter1.1 History , Taxonomy, Morphology of Bacteria
#ApurbaSastry
#Question2- Match the following: Bacterial Classifications with the Principles are used
:
#ApurbaSastry
#Question15- E.g. of Bacteria showing the following
1. Monotrichous flagella
2. Lophotrichous flagella
3. Peritrichous flagella
4. Amphitrichous flagella
#Chapter1.1
#PhysiologyBacterialPathogenecity
14.6.2016
ASM Discussion on FB Round-2 14.6.2016
#Chapter1.1 #PhysiologyBacterialPathogenecity
#ApurbaSastry
#Question1
A.Lag Phase
B.Log Phase
C.Stationary Phase
D.Decline Phase
Which phase of bacterial growth curve the following event occurs
1. Accumulation of enzymes & metabolites
2. Attains max. size
3. Uniformly stained
4. Metabolically active
5. Produce Granules
6. Produce Exotoxin
7. Produce Antibiotics
8. Produce involution forms
#Question2
A.Lag Phase
B.Log Phase
C.Stationary Phase
D.Decline Phase
Which phase of bacterial growth curve the following event occurs
1. Bacteria divide but not die
2. Bacteria do not divide but die
3. Bacteria divide and die
4. Bacteria do not divide and do not die
#Question3
A. Obligate aerobes
B. Facultative anaerobes
C. Facultative aerobes
D. Microaerophilic bacteria
E. Aerotolerant anaerobe
F. Obligate anaerobes
#Question4
A. Bacillus stearothermophilus
B. Pseudomonas
C. E.coli
#Question5
#Question6
#Question7
#Question8
3. Poorly antigenic
4. Heat labile
5. Mostly enzyme like action
6. No effective vaccine is available
7. Specific action
8. Neutralisation by antibodies is ineffective
#Question9
#Question1
1. Sterilization
2. Disinfection
3. Decontamination (or Sanitization)
#Question2
4. Prions
5. Cryptosporidium oocysts
6. Bacterial spores
7. Coccidian cyst
8. Other parasite cysts (Giardia)
9. Fungi
10. Gram-negative bacteria
11. Large non-enveloped viruses
12. Small non-enveloped viruses
13. Gram-positive bacteria
#Question3
Mechanism of action
1. Dry heat
2. Moist heat
#Question4
#Question5
#Question6
Inspissation
Also called as .
Heating an article by
Used for sterilization of .
#Question7
#Question8
2. Membrane filters- They are porous filters that retain particles throughout the filter,
rather than just on the surface.
#Question9
#Question10
Filtration:
1. HEPA filter removes from the air ..% of particles of size ..m .
2. ULPA filters - Removes from the air ..% of particles of size ..m .
3. Sterilization control of membrane filters includes.
4. Membrane filters are made up of
5. Pore size- Membrane filters.
#Question11
6. Cold sterilization
#Question12
#Chapter1.2#Disinfection 16.6.2016
ASM Discussion on FB Round-2 16.6.2016
#Chapter1.2#Disinfection
#ApurbaSastry
#Question1
A. Bacterial spores
B. Tubercle bacilli
C. Non enveloped viruses
D. Fungi
E. Enveloped viruses
F. Vegetative bacteria
#Question2
#Question3
#Question4
#Question5
#Question6
#Question7
1. Endoscopes,
2. Surgical Instruments
3. Stethoscope
4. Computers
5. Implants
6. Eye & dental instruments
7. Respiratory therapy equipments,
8. Anesthesia equipments,
9. Thermometer
10. Examination table
11. Rectal/vaginal /esophageal probes
12. BP cuff
13. Cardiac and urinary catheters
14. ECG electrodes
15. Bedpans
16. Crutches
17. Surfaces of medical equipments
18. Laryngoscope
#Question8
#Question9
Which is the most resistant structure to sterilization and which methods are
recommended for sterilization of this material?
#Question10
TESTING OF DISINFECTANTS:
True or Falser reading Phenol coefficient (Rideal Walker) test
1. E.coli is taken as test organism
2. Phenol is taken as standard disinfectant against which the test disinfectant is
checked
3. Phenol coefficient >10 is taken as is satisfactory
4. Can be used to test any disinfectant
5. It can assess the ability of the disinfectant to act in presence of organic matter.
#Question11
#Chapter1.3
#CultureMediaIdentification 17.6.2016
ASM Discussion on FB Round-2 17.6.2016
#Chapter1.3 #CultureMediaIdentification
#ApurbaSastry
#Question1
#Question2
#Question3
What are the compositions of the following Simple/ basal media (select from the options
given below)?
a) Peptone water
b) Nutrient broth
c) Nutrient agar
1. Peptone (1%)
2. NaCl (0.5%)
3. Water
4. Meat extract (1%)
5. 2% agar
#Question4
#Question5
#Question6
#Question7
D. Anoxomat System
#Question8
#Question9
PRAS media
1. Full form and
2. Where it is used?
#Question10
1. Serotyping
2. Bacteriophage typing
3. Bacteriocin typing4. Biotyping
5. Auxotyping
#Question11
Match the following. Which type of PCR is used in the following situations?
1. For amplifying 6S rRNA genes
2. Double round of amplification
3. Syndromic approach4. Uses >1 primer targeting DNA sequences of several organisms
5. Uses 2 primers: 2nd primer targets the same organism but a different DNA sequence.
6. Quantitative analysis
7. Amplification can be visualized simultaneously during the process of amplification
A. Nested PCR
B. Reverse transcriptase PCR (RT-PCR)C. Multiplex PCR
D. Real-time PCR (rt-PCR)
#Chapter1.3 #CultureMediaIdentification
#ApurbaSastry
#Question12
#Question13
What is the Sequence of steps occurs in a PCR cycle? And what temp all these steps
take place?
#Question14
A. For M.tuberculosis
B. Based on fluorescent based detection technique
C. Based on colorimetry
D. Based on pressure change, detected by manometry
#Question15
Answer the following about the following aspects of the culture media1. Name of the media
2. Type of Media - Basal
/Enriched/Enrichment/Selective/DIfferential/Transport/Anaerobic/for AST
3. Where used
4. Expected growth.
#Chapter1.4 #BacterialGenetics
18.6.2016
ASM Discussion on FB Round-2
#Chapter1.4 #BacterialGenetics
#ApurbaSastry
18.6.2016 Q 1
Property of Plasmid: true or False
1. Extra chromosomal
2. ss linear DNA
3. Cannot replicate independently
4. Can be integrated with chromosome
5. Essential for life of bacteria
6. They may be present always single in number.
7. Curing is The process of eliminating the plasmids from bacteria
#Chapter1.4 #BacterialDrugResistance
19.6.2016
ASM Discussion on FB Round-2
#Chapter1.4 #BacterialDrugResistance
#ApurbaSastry
19.6.2016 -Q 1
The following Resistance is an e.g. of
A. Intrinsic antimicrobial resistance or
B. Acquired antimicrobial resistance
1. S.aureus to Vancomycin
2. Members of Enterobacteriaceae to Chloramphenicol
3. Resistance to Aminoglycosides
4. MRSA to betalactams
5. Streptomycin resistance in Mycobacterium tuberculosis
6. Enterococcus to Vancomycin
7. Rifampicin resistance in Mycobacterium tuberculosis
8. Quinolone resistance seen in S.aureus and S. pneumoniae
9. Staphylococci against macrolides and streptogramins
10. Staphylococcus aureus and Streptococcus pneumoniae against fluoroquinolones