1.

Phosphate buffered saline (PBS)
pH 7.3 1 Liter: 1X: 136.9 mM NaCl 2.7 mM KCl 8 mM Na2HPO4-2H2O 1.47 mM KH2PO4 8g 0.2 g 1.42 g 0.2 g 5X: 40 g 1.0 g 7.1 g 1.0 g 10X: 80 g 2.0 g 14.2 g 2.0 g

3 Liter: 410.7 mM NaCl 8.1 mM KCl 24 mM Na2HPO4-2H2O 4.41 mM KH2PO4 Bring water to 3 L

1X 24 g 0.6 g 4.26 g 0.6 g (Potassium Phosphate Monobasic

Phosphate Buffered Saline with 0.1% Tween 20 (PBST)
For 500 mL: 68.4 mM NaCl 1.35 mM KCl 4 mM Na2HPO4-2H2O 0.735 mM KH2PO4 500 QL of Tween 20 For 1 Liter: 136.9 mM NaCl 2.7 mM KCl 8 mM Na2HPO4-2H2O 1.47 mM KH2PO4 1 mL of Tween 20 4g 0.1 g 0.71 g 0.1 g (Potassium Phosphate Monobasic)

8g 0.2 g 1.42 g 0.2 g (Potassium Phosphate Monobasic)

Transfer Buffer (1L)
25 mM Tris Base 192 mM Glycine 20% methanol ddH2O Transfer Buffer (500 mL) 12.5 mM Tris Base 96 mM Glycine ddH2O 10X (no methanol)* 15.15 g 72 g to 500 mL 1X 3.03 g 14.4 g 200 mL to 1 L 10X (no methanol)* 30.3 g 144 g to 1 L

*Add methanol when diluting 10x solution

Laemmli Running Buffer (1L)
25 mM Tris base 190 mM glycine 0.1% SDS pH 8.3 1X 3.03 g 14.4 g 1g 10X 30.3 g 144 g 10 g

2X Laemmli Sample Buffer (20 mL)
1.6 mL 4 mL 2 mL 0.8 g 0.4 mL 1.5 M Tris-HCl, pH 6.8 glycerol F-mercaptoethanol (FME) SDS 1% bromophenol blue

aliquot and store at -20rC

9. 5X Laemmli Sample Buffer (20 mL)
4 mL 10 mL 5 mL 2g 1 mL 1.5 M Tris-HCl, pH 6.8 glycerol F-mercaptoethanol (FME) SDS 1% bromophenol blue

aliquot and store at -20rC

10. LB (1 Liter)
25 g LB Powder* Add dIH2O to 1 L *Wear mask when measuring

11. LB/Agar
1 Liter: 25 g LB Powder* 20 g Agarose Add ddH2O to 1 L 200 mL: 5 g LB Powder* 4 g Agarose Add dIH2O to 200 mL

12. Coomasie Blue Stain (50 mL)
0.1 % Coomasie Blue 0.05 g 50% methanol 25 mL 10% glacial acetic acid 5 mL ddH2O to 50 mL Dissolve Coomasie Blue in Methanol first, then add acetic acid and water.

13. Coomasie Blue Destain (500mL)
20% Methanol 7.5% Glacial acetic acid dIH2O 125 mL 37.5 mL 337.5 mL

15. Ampicillin Stock -100 mg/mL (10 mL)
1 g sodium ampicillin (MW=371.4) Add ddH20 to 10 mL

16. Kanamycin Stock

17. 10X TBE Buffer
Tris Base Boric Acid EDTA dIH20 1L 108 g 55 g 9.3 g to 1 L 2L 216 g 110 g 18.6 g to 2 L 3L 324 g 165 g 27.9 g to 3 L

18. 1X TBE Buffer
Tris Base Boric Acid EDTA dIH20 1L 10.8 g 5.5 g .93 g to 1 L 2L 21.6 g 11.0 g 1.86 g to 2 L 3L 32.4 g 16.5 g 2.79 g to 3 L

19. TBS-T
Tris Base NaCl Tween-20 Tris-HCl 1x 0.6 g 8g 0.5 mL 2.37 g 10x 6g 80 g 5 mL 23.7 g