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Hydrolysis of Carbs

Hydrolysis of Carbs

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Published by: Santana Janai Francisco on Sep 20, 2010
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10/21/2013

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Santana Francisco Dr.

Buzingo 9/12/2010

Hydrolysis of Carbohydrates Lab Introduction: Carbohydrates are macromolecules that mostly contain carbon,oxygen, and hydrogen. The simplest form of a carbohydrate molecule is called a monosaccharide also known as a simple sugar. When two of these monosaccharides come together, they make disaccharides, and if long chains of monosaccharides become linked, then they form polysaccharides. Disaccharides and polysaccharides can be broken down back into monosaccharides in a process called hydrolysis in which water is added so that such solutions can be broken down. In this lab, we put hydrolysis to the test by trying to break down two different carbohydrates: starch and sucrose. If we use hydrolysis to break down starch and sucrose, then in sucrose we shall see it break down into glucose or fructose and in starch we shall see it break down into unites of glucose. Materials and Methods: In this experiment, you will need eight test tubes, a test tube rack, two extra large test tubes and a beaker to hold these test tubes if they don t fit in the rack, a hot plate, a sucrose solution, starch solution, water and hydrochloric acid (HCL). Once you gather these materials, you will get the eight test tubes and label them 1 through 8 as you line them in order on the rack. Grab the two extra large test tubes and label them starch and sucrose. You will now put 10 mL of starch solution and 5 mL of HCL into the test tube you labeled starch. Then move on to the sucrose test tube and put 10 mL of sucrose solution and 2 mL of HCL into that test tube. After this has been accomplished, simultaneously swirl each tube to mix the contents. Then use a pipet to draw 1 pipetful of solution from the sucrose tube and put it in Tube 1. Grab a different pipet and draw 1 pipetful of solution from the starch tube and put it in Tube 3. Do the same and put it in Tube 4. Then place both the sucrose and starch extra large test tubes in your boiling water bath on the hot plate. After 2 or 3 minutes draw 1 pipetful of solution from the sucrose tube and put it in Tube 2. You are now done with the sucrose solution and may remove it from the water bath. After 5 minutes, draw 1 pipetful of solution from the starch tube and put it in Tube 5. Do the same and put it in Tube 6. Wait 10 more minutes and the repeat the previous steps for Tubes 7 and 8. Now you will move on the adding reagents to the test tubes. Add two droppersful of Benedict s reagent to Tubes 1,2,3,5, and 7 and place these tubes in the boiling water for 5 minutes. Then add 3 or 4 drops of iodine reagent to Tubes 4, 6 and 8. Record the results from these tubes in your table. After 5 minutes has passed, remove Tubes 1,2,3,5, and 7 from the water bath, wait 5 minutes for them to cool and then record the results in your table.

in Tube 2 after being in the hot water bath for 2-3 minutes. The starch was probably not left in the hot water bath long enough to actually hydrolyze and break down into smaller sugars which is why I get the negative result. the benedict reagent reacted with the broken down sucrose to produce an orange solution that indicates it s positive for monosaccharides. Sucrose is only a disaccharide which means there is only the breaking down of two monosaccharides instead of a chain like in polysaccharides. This is the normal result because Benedict s doesn t react with all small sugars. my results all came out negative (benedict s reagent remains light blue) showing that the benedict s found no monosaccharides or disaccharides in my hydrolyzed starch solution. When using the Benedict s solution on the sucrose.results: Starch and Sucrose Test Tubes Test Results when Benedict s and Iodine are Added Conclusion/Discussion: Hydrolysis was successful in this lab as we were successful in breaking down sucrose and into monosaccharides but not in starch. Then when using the benedict s reagent to test the starch for the broken down sugars. Hydrolysis . This hydrolysis of polysaccharides is called saccharification (Wikipedia). These results are backwards in actuality showing that I may not have the extra large tube of starch in the boiling water bath long enough which is why I m still getting a positive result of starch for Tubes 6 and 8. Hydrolysis is important because the breaking down of a polysaccharide can lead to the production of many monosaccharides and disaccahrides like maltose which can be used as yeast to make beer (Wikipedia). Tube 4 probably tested negative because I probably didn t place starch in this Tube or I confused it with Tubes 6 and 8 and it gave me the hydrolyzed substance I needed. Hydrolysis proved effective in this case as we went from a negative reading to a positive reading after using hydrolysis to break down sucrose. however. This is because starch is a polysaccharide which is a long chain of monosaccharides and it would logically take longer for it to break down into simple sugars that can actually be detected in the different reagents. When testing the starch with the iodine reagent my results showed Tube 4 testing negative for starch but Tubes 6 and 8 testing positive for starch. We see positive results in the testing of sucrose but not in starch because it takes longer for starch to hydrolyze. Test tube 1 showed no change with sucrose still being read as negative for monosaccharides or disaccharides. including sucrose. The remaining sugar is either fructose or glucose.

References: .com).is an organic way in which our bodies can break down and digest food which is why it is so important (Yahoo.

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