PHARMACEUTICAL ANALYSIS UNIT I Components of pharmacopoeia monographs, for raw materials and finished products UNIT II Principle and

procedures involved in following, including assays of official drugs in I.P by non aqueous titration, Complexometric titration, gravimetric methods, diazotization titration, potentiometry, UV Visible method, HPLC & TLC UNIT III A detailed study on related substances and impurities present in drugs and their effect on drug stability and therapeutic action. ICH guidelines for impurities and related substances determination in drugs. UNIT IV Identification and quantitative determination of preservatives, antioxidants, coloring materials, emulsifiers, and stabilizers in pharmaceutical formulation.Quality control and in process quality control of tablets, capsules, liquid dosage forms, parentral and sterile preparations and ointments, creams, suppositories and contolled release products. UNIT V Quality control of containers and closures as per I.P, B.P, USP – Plastic containers, glass containers, rubber closures etc.Water analysis: Physical, chemical, and biological analysis of effluent COD & BOD.

PRACTICAL The practical syllabus comprises of the exercises based on the topics mentioned in the Theory syllabus. REFERENCE: 1. A.H. Beckett and J.B. Stenlake, Practical Pharmaceutical chemistry, psrt 1&2, the athlone press, London. 2. Pharmacopoeia of India, Govt. of India, Ministry of Health. 3. British Pharmacopoeia, ministry of health and social welfare, UK. 4. Textbook of Pharmaceutical chemistry by Bentley & Driver
5.

Fundamentals of analytical chemistry 7th edition: skoog, west; holler.

6 Handbook of Microbiological Quality control, Stephen Denyer, W. B. Hugo, A. D. Russel, Norman A. Hodges, Sean P. Gorman,

Blackwell publishing, 2003.

COSMETIC ANALYSIS UNIT 1 Classification of raw materials and raw materials used in the cosmetic industry for the manufacture of finished products. Method of sampling, Indian Standard specification laid down for sampling and testing of various cosmetics in finished form by the bureau of Indian standards. Unit II

hair creams.Brief introduction of the following cosmetic preparation and a detailed study on their quality control: Shampoo. Denny (Rev. A. Toxicity tests for cosmetics Unit IV Radiochemical Analysis . 4th Edition by Leon Shargel / Andrew B. Unit III General methods of analysis to determine quality of raw materials used in cosmetic industry. toiletries etc. and their analysis. lipstick and hair dyes.Instruments used . J. nail polish. 2. 4th Edition. skin powder. and Positron Emission Topography (PET). Yu – 1999. Jeffery. neutron activation. Souney. C. H. Applied Biopharmaceutics and Pharmacokinetics. lipstick. Paul F. Larry N. B. Basset. by) Vogels Text Book of Quantitative Chemical Analysis. Part I and Part II. Beckett and J. hair products nail polishes. G. Unit V Standardization of cosmetics. J. depilatories.C. after shave lotion.analytical and screening instruments. lotions. REFERENCE: 1. 3. powders. ELBS. Alan H. Mutnick. skin creams. bath and toiletries. 5th Edition 1989. Comprehensive Pharmacy Review 5th Edition by Leon Shargel. Information on ingredients used in various cosmetics such as creams.Stability testing of cosmetic formulation and shelf life prediction. depilatories. R. ICH guidelines for stability studies of formulation. H. Mendham. Tooth paste. 4. Stenlake Practical Pharmaceutical Chemistry. perfumes. . Sawnson – 2004. Isotopic dilution..

ICH guideline for impurity determination and stability studies. Quantitative Analysis of Drugs in Pharmaceutical Formulations. Sethi. I and Vol. UNIT – II Flame photometry and atomic absorption spectrophotometer. Joseph I. solvent effect. Non conjugated interactions. P. Snyder. 7. Govt.D. II . 6. wood ward Fieser. Beer and lambert’s law limitations of the law. New Delhi. Vol. The Controller of Publications. Longman Scientific and Technical Publishers. Theory. 3rd Edition . John Wiley and Sons 2nd Edition – 1997 INSTRUMENTAL ANALYSIS UNIT – I Uv visible spectrophotometry – Brief review of electromagnetic spectrum and absorption of radiations. Moore : Herry’s Cosmeticology. Joseph J. molecular ion and metastable peak. Singapore. Indian Pharmacopoeia. stereo chemical effect. Glajch. spectral correlation with structure. UNIT – III . energy levels and selection rules. of India. J. Feieser kuhn and nelson rules. application of spectrophotometry inqualitytative and quantitative analysis. ring size and strain on spectral characteristics.1997. electron and chemical ionization modes. influence of substituent. Mass spectrophotometry. J. application. 8. B.5. Practical HPLC method development by Lloyd R. 9.1996. Wilkinson and R. Mc lafferty rearrangement. Kirkland. fragmentation patterns nitrogen and ring rules.

4th Edition • Textbook of Pharmaceutical chemistry by Bentley & Driver . effect. Peri. Vaccum Liquid Chromatography. quadrupole broadening and decoupling. London • current chromatography. double resonance. Bay effects.Nuclear Magnetic Resonance Spectrometry: Magnetic nuclei. bonded phase. normal and reversed phase. Pascal’s triangle. spin decoupling. Coupling constant. Centrifugal affinity chromatography. virtual coupling. UNIT – IV Chromatography. shift reagents – mechanism of action. PRACTICAL The practical syllabus comprises of the exercises based on the topics mentioned in the Theory syllabus. Spin – spin splitting. Over Pressure counter Layer – Chromatography. UNIT – V TLC. B. Longman. separation mechanisms General – principleas. High Pressure Liquid Chromatographpy. techniques. A. Stenlake Part I and Part II. chromatography. size exclusion an dion pair chromatography Practical Pharmaceutical Chemistry. of conformations and stereochemistry on the spectrum. Beckett and J. chemical shift and shielding and magnetic anisotropy. High pressure Thin Layaer chromatography. classification and of Flash chromatographic. diasteromeric protons. H. mechanism of coupling. long range coupling – Epi. column chromatography chromatography. REFERNCES • Vogel Text Book of Quantitative Chemical Analysis.

west. Saunders college publishing . diastereomers and meso compounds.H.g.. New york. J. Skoog and leary J.A. clinical trials. • Instrumental methods of Analysis.A.A. chemistry and lead identification.L. use stereochemical projections (e. understand the terms enantiomers. UNIT – II Historical development of pharmaceutical chemistry Sources of new drugs Drug nomenclature Structural factors affecting biological activity Physicochemical properties in relation to biological activity UNIT – III Molecular stereochemistry: Geometrical isomerism .importance of optical isomerism to drug action. Meritt.different conformations of alkanes and . Settle Wadsworath .• • Fundamentals of analytical chemistry 7th edition: skoog. development of lead structures. S configurations to label optical isomers. Dean and F. use R.importance of geometrical isomerism to drug action. Willard.J. holler. L. Fischer and Newman projections) to represent organic molecules. use the E. Principles of instrumental analysis. Optical isomerism . D. Conformational isomerism . New york ADVANCED PHARMACEUTICAL CHEMISTRY UNIT – I General strategies for drug development: Biology of disease. understand the concepts of optical activity and optical purity. H. Z nomenclature to label geometrical isomers.

partial least square analysis (PLS) and other multivariate statistical methods. Physicochemical parameters. Pakistan. London. Royal Society of Pharmaceutical Press.. Arayne M. 2.. Saeed. relationship between them.Hansch analysis. University of Karachi. gastro intestinal absorption. transdermal absorption. parenteraladministration. rationale of prodrug design and practical considerations. distribution. REFERENCE: 1. chemical bond.. 3D QSAR approaches. . BCCT. Karachi. understand the energy barriers to rotation and interconversion among conformational isomers UNIT-IV PRODRUG DESIGN. PRACTICAL The practical syllabus comprises of the exercises based on the topics mentioned in the Theory syllabus. Manual of Qualitative Organic Analysis.cycloalkanes. UNIT-V Quantitative analysis of structure activity:Relationship History and development of QSAR.Introduction. British Pharmacopoeia. Stationary Press. Drug receptor interactions. Fee Wilson analysis. Statistical methods – regression analysis. pharmacokinetic andbiopharmaceutical aspects.

Mack Publishing Company. FTIR – theory. Unit – II SPECTROFLUORIMETRY: . United State Pharmacopoeial Convention. IR – Spectrometry: Theory. absorption law and limitations. Eston. instrumentation. modern instrumentation – design and working principle. Fiesev D.D. molecular vibration. Health and Company. Lovis F. Theory of electronic spectroscopy. instrumentation and its application. Twinbrook Parkway. applications of IR Spectrometry in pharmacy. Modern Pharmaceutical Analytical Techniques – Theory UNIT – 1 UV-VISIBLE SPECTROSCOPY : Brief review of electromagnetic spectrum and absorption of radiations. 20852 USA. 12601. USA. Rockyville M. 5. absorption by organic molecules. London.. Applications of UV-Visible spectroscopy (qualitative and quantitative analysis). Vogel Text Book of Quantitative Chemical Analysis. Inc. 6. choice of solvent and solvent effects. Woodward – Fischer rules for calculating absorption maximum. United State Pharmacopoeia. 4. Remington Practical of the Science and Pharmacy. Longman.3. The chromophore concept. Experiments in Inorganic Chemistry. Pennsylvania. Boston. sample preparation.C.

chemical shift. field desorption. Mass spectrometry: Theory. proton exchange reactions.Theory. Instrumentation. Natural abundance. factors affecting fluorescence intensity. Fragmentation pattern. interpretation of spectra. instrumentation. NOESY. advantages. Interpretation of mass spectra. solvent effect. FT-NMR. NMDR. COSY and applications in Pharmacy. C13 NMR-Introduction. and factors influencing the value of coupling constant. comparison of fluorescence and UV-Visible absorption methods and applications in Pharmacy. ionization techniques. C13 NMR Spectra and its structural applications. effect of acids and bases on fluorescence Spectra. spin – spin coupling. 2D -NMR. NOE. relationship of chemical structure to fluorescence spectra. coupling constant. solvents. concentration effects. UNIT-111 NMR . and factors affecting chemical shift. chemical shift. Interpretation of NMR spectra & applications of NMR spectrometry in pharmacy. determination of molecular weight and molecular formula. fast atom bombardment and application of mass spectrometry. electron bombaratment. chemical ionization. spin-spin decoupling. Magnetic equivalence – spin – spin decoupling – shift reagents.Theory. instrumentation. spin-spin coupling.SPECTROMETRY: Fundamental Principles and Theory. UNIT – 4 CHROMATOGRAPHIC TECHNIQUES .

c) High Performance Liquid Chromatography: Principle. RP-HPLC. b) Gas Chromatography: Theory and principle. column chromatography and affinity chromatography – techniques and applications. Pharmacy. derivatisation methods and applications in . thin layer chromatography. instrumentation. instrumentation. elution techniques and pharmaceutical applications. PRACTICAL The practical syllabus comprises of the exercises based on the topics mentioned in the Theory syllabus. d) HPTLC and Super Critical Fluid Chromatography (SFC): Theory and Principle. column operation. UNIT – 5 Theory. ion exchange chromatography. interpretation of diffraction patterns and applications. xray powder diffraction. LC-MS and applications in Pharmacy. x-ray diffraction. generation of x – rays. solvents used elution techniques. instrumentation and application of atomic absorption and atomic emission spectroscopy. instrumentation. Bragg’s law. X – Ray diffraction methods: Introduction.a) Classification of chromatographic methods based on mechanism of separation: paper chromatography.

Stenlake. Sharma.K. microscopical. Y. London. Skoog and leary J. company Ltd. Jain chemicak analysis.B.REFERENCE 1. Introduction to molecular spectroscopy. 3. Srivastava. 7. Saunders college publishing. New Delhi. D. Principles of instrumental analysis. London. H. 6. s. Haris. P. cytomorphological and chemical examinations of raw materials and finished products. 5. London. Elementary organic absorption spectroscopy. 4. Practical Pharmaceutical chemistry. Pitman. UNIT-II S. R. Instrumental methods of Analysis.H.C. Dean and F. Nuclear Magneatic Resonance spectroscopy.A. the athlone press. McGraw – Hill. New york. Settle. psrt 1&2.A. STANDARDIZATION AND STABILIZATION METHODS THEORY UNIT-I WHO guide lines of the standardization of Herbal raw materials and finished products. Wadsworath . 2. New Delhi.K. Chand & .H. Chand.. J.J. L. Willard.L. A. A. Meritt. New york.. Beckett and J.A.H. Morphological.

sex. UNIT-V Factors affecting stability of a formulation. bitterness values. lotions. excipients and their estimation by different techniques. and physical constants applicable to the lipid containing drugs. ICH guidelines. Physicochemical characterization in whole form. Concepts of nutritional requirements at different age. volatile oil content. depilatories. UNIT-IV Standardization of cosmetics. moisture content. toiletries etc. Information on ingredients used in various cosmetics such as creams. PRACTICAL The practical syllabus comprises of the exercises based on the topics mentioned in the Theory syllabus. jaundice etc. Concept of development of stability indicating analytical methods. ash values. Methods of stabilizations and Methods of stability testing. Standardization of Herbal products. bioburden and Pharmacopoeial microbial assays. foreign matters.Determination of Physical and chemical constants such as extractive values. hypertension and atherosclerosis. . UNIT-III Standardization of food products. pregnancy and diseases like diabetes. and in different conditions like normal. Different types of additives used and analysis of these ingredients in ethical and non ethical foods. hair products nail polishes.Microbial counts. and their analysis. lipstick. powders. separation and identification of active principles. alcohol content.

R. Indian herbal pharmacopoea. The practical evaluation of phytopharmaceutical by brain & turner 17. Marsel deckker.s cosmeticology. 11. 9. 15. Funke. Applied Microbiology. Borc 5. Vinitakale Kishor Bhusari.An introduction. Herbal cosmetics. Food additive. 4. “ Introduction to chemical analysis of foods. Manufacturing and Quality control.Alfred larry branen. 12.Vol-I & II . Cosmetic analysis. The chemical analysis of foods and food products.P.selective methods and techniques by P. Cosmaceuticals Drug vs Cosmetics 7. Henry. Rieger. 5th edition. Tortora. 16. S.” Microbiology”.Sharma. Taylor 2.Cosmetics Formulation. Michael J. Pelezar/ Chan/ Kricg. 2nd edition. “ Microbiology. Suzanne Neilson.. 8th edition. Jacobs. 6. 14.Dr. Method of protein analysis by istran kerese. Case. Beuty through Herbs. Drug stability.BOOKS RECOMMENDED 1. Jemns T Cartenson. WHO Guide line for the quality control of herbal plant material. 13.Martin M. J. P. P Michael division publishing corporation 3.’ 10. 8. Morris B. Antimicrobial in food. Urjita jain.Principles and Practices.

Phenylpropanolamine Hcl B. quality control. Unit II: Raw materials: Establishment of specifications. Assay validation during product development phase.Semester Advanced Pharmaceutical Analysis Unit I Applications of optical and electrical instruments used in pharmaceutical analysis.P. test procedures.Dissolution. identification and quantitation of a natural drug substance by chromatographic methods such as TLC. production.P. B. stability. glycosides. identification and purity determination of the following important pharmaceutical compounds as mentioned in I. sampling procedures. Bioavailability evaluation of drugs and formulations. Ibuprofen B. Acetysalicylic Acid B. antibiotics. Separation. GLC. Applications of polarography in pharmaceutical analysis. Diclofenac Potassium B.P. analgesics. sulphonamides.P. Unit III Assay of pharmaceutical compounds (minimum10 compounds) based on spectrophotometric methods. A comparative study of the advanced physicochemical and chemical methods in analysis of drugs and pharmaceutical preparations as exemplified by vitamins. P. clinical and medicinal applications of radioisotopes used in pharmacy and medicine preparations of diagnostic and therapeutic agents .P.P Salbutamol Sulphate I. HPLC UNIT IV Preparation/synthesis.P. Regulator requirements. alkaloids. Chlorzoxazone USP.P Aminophylline Tablets I. antihistamines. S. Methacarbamol USP. Dicyclomine I.II. P and U.. Niacinamide IP UNIT V Radiopharmaceuticals Basic properties.

b. recovered materials and reprocessing. I. Unit – II Philosophy of peer evaluation and accreditation. Organic Spectroscopy. and Brochmann Hanssen E .Backet and Stenlake.S.C. Quality control laboratory: GLP. Fundamental of Molecular Spectroscopy.Creaser C. A Textbook of Pharmaceutical Analysis. NABL and OSHA (ISO 18000) Unit – III . Sandell. US FDA guidelines for GLP in non clinical testing laboratories ( only salient features will be covered). Practical Pharmaceutical Chemistry. Watson D..J. organization and accreditation bodies – ISO 9000. ISO – 14000. and Davies A. Instrumental Methods of Chemical Analysis.. Conners K.. sampling plan.. distribution and distribution records – handling of returned goods. Student and Pharmaceutical Chemistry.Higuchi T. Meehan. E.B.G Churchill Livingston (1999).Longman A Textbook of Pharmacy. Analytical Application of Spectroscopy. Eving QUALITY CONTROL AND QUALITY ASSURANCE Unit – 1 a.N. E.Books for references: • • • • • • • • • • Vogel Text Book of Quantitative Chemical Analysis. Quantitative Chemical Analysis-Kolthof. -Banwell C.M.M.A 2nd edition.William Kemp.Golden W. Pharmaceutical Analysis..

validation of equipment and facilities in sterile and non sterile areas. compression. hygiene. validation of air handling. Bubble packs. A guide to Total Quality Management – Kaushik Maitra and Sedhan K. coating. Good Laboratory Practice Regulations. breakable seals and sealed tubes. Types ofplastics used. 69. protocol for process validation. Organisation and personnel. membrane filtration etc. Standard operating procedures for various operations like cleaning. tape seals.Ghosh.Decker Series. cartonsand other packaging material. disinfection. Different types of closures and closure liners. leaching. Unit – V Packaging and labeling controls. training. Quality control of packaging material and filling equipment. reconciliation of labels. types and tests assuring quality of glass. 2nd Edition.Concepts of validation – types of validation. cleaning validation. drying. Blister packs. Quality Assurance of Pharmaceuticals – A compendium of guidelines and related materials – Vol. chemical reactions.modification of plastics by drugs. b. film wrapper. Unit – IV a. filling. sterilization. I – WHO Publications. Vo. line clearance. P. foil / plastic pouches. . Sandy Weinberg. responsibilities. shrink handling. Sharma. REFERENCE: • • • • • • Quality Assurance Guide by Organisation of Pharmaceutical products of India. biological tests. bottle seals. sorption. permeation. How to practice GMPs – P.

Alkaloids: Structure and synthesis of some important alkaloids. Ghosh. cephalosporins. identification and application of GLC. carbapenems and penems. CHEMISTRY OF NATURAL PRODUCTS UNIT I Isolation. extraction. spectral properties configuration and reactions of steroids. MS. isolation. dioscorea. ginseng. biosynthesis of vitamins and discussion about halibut oil. e. pharmacology and medicinal uses of glycyrrhiza. vitamin B-Complex i.e. aloe. Steroids: Synthesis. ruta etc. UNIT III Terpenes: Chemistry of important terpenes from various group Humulene . Application of IR H1 NMR. Amino glycosides. cod liver oil. cascara. linomycins and polypeptide antibiotics. nocardicins and monobactums. rhubarb. UNIT II β – LACTUM AND NON –β LACTUM ANTIBIOTICS. thiamine. biosynthesis of sterols. and Flavonoids. Germacrone etc. chemistry. dried yeast.• ISO 9000 and Total Quality Management – Sadhank. senna. HPLC and counter current distribution to separation and analysis of plant constituents. ORD and CD to structural studies of natural products. β-lactamase inhibitors and other β-lactum agents.g . UNIT V Vitamins Introduction. classification. UNIT IV Glycosides: Introduction. hormones. growth regulators. penicillins. Mechanism of action. classification. G. macrolides.

cyanocobalamine. Chapmannstall. Burger’s Medicinal Chemistry. folic acid. Agarwal. and K. Finar Elements of Biotechnology by P. Phytochemical methods of Harborne. I to IV – Scikel Runeckles. Chemistry of natural products Vol I onwards IWPAC.riboflavin. Phytochemistry Voi. Biotechnology by Purohit and Mathoor. Organic Chemistry of Natural Products Vol I and II by Gurdeep and Chatwall.L. Jan Nostrant Rein Hld. E.P. Natural Product Chemistry Nakanishi Gggolo. pentothenic acid.K. Natural Product Chemistry “A laboratory guide” – Rapheal Khan. Books for reference: • • • • • • • • • Modern methods of plant analysis – Peech and M.Vyas and V. • • • • • • • Organic Chemistry of Natural Products Vol I and II by O.Tracey.K. A. D. Introduction to molecular Phytochemistry – CHJ Wells.Dixit. Gupta. The Alkaloid Chemistry and Physiology by THF Manske. vitamin C.P. Recent advances in Phytochemistry Vol. niacin and niacinamide. Pharmaceutical Biotechnology by S.V. INTELLECTUAL PROPERTY RIGHTS AND REGULATORY AFFAIRS Unit – I . Organic Chemistry Vol I and II by I. I and II by Miller. biotin.

Unit – II Trade related aspects of intellectual property rights. TePP. factors affecting choice of intellectual property protection. Unit – V a. trade mark. Gazette of India. economic importance. b. NRDC. Trade related investment measures (TRIM). related registration and marketing issues. Managing technology transfer – guidelines for research students. business models. filling of patent application. drug related technology development. international patenting – requirements procedures and costs.Need for the introduction of regulatory affairs in the pharmacy curriculum. Unit – IV Technology development / transfer/ commercialization related aspects. property protection and intellectual property rights. copyrights. Drug master file submission. concepts behind world trade organization (WTO). TDB schemes. . financial assistance for patenting. role of intellectual property in pharmaceutical industry. precautions before patenting disclosures / non disclosures. HGT. Funding sources for commercialization of technology: Preparation of project report. echo responsibility – economic. toxicological studies. social responsibility. SOPs. GOI schemes and incentives. Positive and negative aspects of IPP. mechanism for protection of intellectual property – patent. status in Europe and US. publications of patents. social and environmental benefits of modern biotechnology. Unit – III Nuts and bolts of patenting. General Agreements on tariff and trade. avoiding unethical practices. World intellectual property organization.

fennel. H. steroids. digitalis. terpenes and flavonoids. Evaluation and Adulteration of Drugs The organoleptic. spoilage. copyright & trademark: An Intellectual property desk reference. Wagner's reagent test. Patent. saponins. copyright and trademark. Nolo publishers. cinnamon. hyocyamus. TRIPS and developing countries. official and non-official drugs. coriander. Stephen Elias. Richard Stim. 2006. tannins. 2004 Patent strategy: for researchers and research managers. Nolo. glycosides. Termeric. Leaf: senna. john wiley. Sweet and Maxwell. copy right. alkaloids. Mayer's reagent test. Rheum. UNIT III Preliminary Screening of Natural Products:Preliminary chemical tests for the detection of carbohydrates. chemical and therapeutic classification. sophistication and substitution. admixture. UNIT II Powdered drug examination: Cinnamon bark. 20001 Intellectual property: patents. nux vomica seeds. Alkaloids. Rhizome: Ginger. senna leaf. oxford university press EVALUATION OF CRUDE DRUGS UNIT 1 Crude drugs. atropa. The WTO and India’s Pharmaceutical industry – paten protection. . 2003. Seed: Castor.Books for reference: • • • • • Patent. sudip chaudhuri. Belladonna Glycyrrhiza and Rauwolfia. datura and catharenthus. Bark: Cascara. Fruit: piper. Rodolph Cornish. commerce and preparation of drugs. 5th edition.Histological examination by section cutting: Transverse sections of fresh and crude drugs. trade marks and allied rights. Types of adulteration. . microscopic and physical evaluation of the drugs. Jackson Knight. inferiority. eucalyptus. clove flower bud. David Liwelyn. Root: Aconite. coriander. Flower: clove flower bud. Raichard stim. pippermint. Stem: Ephedra.

lead acetate test UNIT IV Phytochemical Examination of Natural Products Leaves:digitalis..V. Meyer. castor.Assay of pepermint oil for total esters. Barks: Cascara.Determination of volatile oil in Cinnamon. datura. Encyclopedia of Plant physiology Secondary Plant Products (1980).Tannins. cinnamomum. froth test. haemolysis test.J. and Bladt S. 5.R. fennel. Salkoawaski test. Laboratory hand book for the fractionation of natural products (1998). Berlin (1996). cyanidin test. colour test. coriander. Plant Drug Analysis 2nd edn: Springer. eucalyptus. Leibermann-Burchard test. Hughton P. F. and Bott T.B. atropa.Saponification value of cotton seed oil. Saponins. Flavonoids.Assay of benzoin.Isolation of shikimic acid from Illicium anisatum. • • 4. rauwolfia.Dragendorff's reagent test. Sterols. Practical High Performance Liquid Chromatography (1999). Glycosides. Roots: belladonna.Isolation of menthol from Mentha piperita. • 6. . Kedde reagent test. • • Harbone J. ferric chlorides test. Fruits and Seeds: Clove. Harlwood B.Colour reactions of tannins. King M. Reference Books • Wagener H. V. B. Sanguineria.A.. senna. Bell. UNIT V Identification of fixed oil by colour reactions. Keller killiani test.Iodine value of olive oil. Stem: Senna. Extraction of Natural Products Using Near Critical Solvents (1993).To determine the acid value of rosin. Rhizome: Podophyllum. . Phytochemical Methods (1998).

ANOVA (one way). E. • Goodwin T. The Biochemistry of Plants Secondary Plant Products (1981). Alkaloids Vol. normal and binomial distributions and their significance b. Wilcoxon signed rank test. and Basha A. Probability.• Manske R.F.U. contingency tables.V-XVI Academic Press. Unit – 3 The population – the sample.H. Rahman A.W. significance of correlation and regression. Hypothesis testing – t tests. Stumpt P. Measurement of spread of data. Runs test for randomness. Unit – 5 .W.. Measures describing the center of data distributions. Chi squared test. New York (19551977). sampling techniques. Biosynthesis of Plant Sterols and other Triterperoids (1981). statistical procedures in assay development.E.sign test. F tests. Unit – 4 Bio assay. Linear regression and correlation. 1-3 (1985). dose – effect’s relationships. precision and accuracy. Unit – 2 a. process control. Probability. • • • Pellwtier S. Statistical inference – statistical estimation. calculation of LD50 & ED50. schwhart control charts.K. statistical quality control. and Conn. BIOSTATISTICS & COMPUTER APPLICATIONS Unit – 1 An introduction to statistics. Biosynthesis of Indole Alkaloids (1983). The Alkaloids Vol. definition and probability distributions.

DBMS. Richard. concepts. foxpro. Amsterdam. steps involved in software development. Application of computers in pharmacy. J. basic units and functions. Drug and pharmaceutical series volume – 80. hardware. P.S.2000 Pharmaceuticals statistics by Sunford Bolton.. Dochain. C languages & programming. 2002 . Internet. 3rd edition. Computer application in Pharmaceutical Research and Development Computer application in Bio technology. software opearating system. Sundar Rao. D. Programming in Ansi C. CDROM. 2003. b. 2nd edition by E.a.S. Elsevier. Fundamentals of computers. Prentice – hall of India. Marcel Dekker Inc. REFERENCE • • • • • An introduction to Biostatistics : A manual for students in health sciences. Balaguruswamy.

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