Figure 1: Classifications

Body Contact intact skin mucous membranes sterile body cavity Disinfection Requirements low level high level sterilization FDA Device Class non-critical semi-critical critical

Figure 2: Microorganism's Resistance to Disinfection and Sterilization Associated with Disinfection Level Classification High-Level Disinfection or Sterilization Bacterial Spores (Bacillis subtilis, Clostridium difficile) Intermediate-Level Disinfection Tubercle Bacilli (Mycobacterium tuberculosis) Nonlipid and Small Viruses (Coxsackievirus, Hepatitis A Virus, Poliovirus, Rhinovirus) Low-Level Disinfection Fungi (Aspergillus niger, Candida albicans, Trichophyton mentagrophytes) Gram Positive & Gram Negative Vegetative Bacteria (Pseudomonas aeruginosa, Salmonella choleraesuis, Staphylococcus aureus) Lipid or Medium-Sized Viruses (Cytomegalovirus, Hantavirus, Herpes Simplex Virus, Hepatitis B Virus, Human Immunodeficiency Virus-HIV 1, Respiratory Syncytial Virus)
Chart modified from Favero and Bond, 1991

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