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Enzyme Technology (Lecture 6)

Enzyme Technology (Lecture 6)

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11/15/2012

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INDUSTRIAL & ENVIRONMENTAL BIOTECHNOLOGY Course # KIBGE-707 KIBGE

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AFSHEEN AMAN, Ph.D. Assistant Professor Industrial Biotechnology Section Dr. A.Q. Khan Institute of Biotechnology & Genetic Engineering (KIBGE) University of Karachi

ENZYME ISOLATION & ENZYME TECHNOLOGY
Course # KIBGE-707 KIBGE-

ENZYME TECHNOLOGY 
The use of purified enzymes for generating a useful product or service constitutes enzyme technology. HISTORICAL BACKGROUND ‡ 1833: first observation of enzyme activity in a test tube was reported by Payen and Persoz ‡ 1878: The term 'enzyme' was introduced by Kiihne ‡ 1890: Fisher suggested the 'lock and key' model of enzyme action ‡ 1913: mathematical model of enzyme action was proposed by Michaelis and Menten ‡ 1926: Sumner crystallized, for the first time, an enzyme (urease)

Continued.....
‡ 1948: The transition state theory of enzyme action was put forth by Pauling ‡ 1951: Pauling and Corey discovered the X-helix and sheet structures of enzymes ‡ 1953: Sanger determined the amino acid sequence of a protein (insulin) ‡ 1986: Cech discovered catalytic RNA, while Lerner and Schutlz developed catalytic antibodies

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