Lab Report

Purpose: The purpose of this experiment is to determine and observe the difference between an animal cell (cheek cell) and a plant cell (onion cell). Also the purpose was to try to determine if one can see the cell better with water or methylene blue under a microscope. Hypothesis: The hypothesis is believed to be that one may be able to see the cells better with the methylene blue rather than water. It is crucial for the methylene blue to colour the cell because the onion cell is sort of transparent. So if one were to spot the onion cell with water, it would be more difficult to spot this cell because it’s also transparent. Also the cheek cells are surrounded with saliva and salvia is transparent just like water. Hence it will be difficult to spot without the methylene blue, as it will colour the cell, making it more visible. Finally the animal cell may not have an actual shape because it has no cell wall, which usually gives the cell some shape. The plant cell will look sort of like a rectangle but not a perfect rectangle because it has a cell wall and on the diagrams I’ve seen, they show what shape the plant cell is going to have. Materials: The materials that are needed to perform this experiment are a microscope, slide, cover slip, water, eyedropper, toothpick, tweezers, methylene blue and an onion. Procedure: For the cheek cells with water: A) Gently scrape with a toothpick at the inside of your cheek and then roll it on to the slide. B) Let the slide dry for 5 minutes and then using the eyedropper, put about 3 drops of water at the spot where you rolled the toothpick. C) Drop the cover slip on to the water at a 45° angle so that there is very small amount of air bubbles. D) Move the slide onto the stage and adjust the fine and course adjustment knob until the cells are visible. For the cheek cells with methylene blue, do the same steps as the cheek cells with water but instead of adding three drops of water, add three drops of methylene blue. A) For the onion cell, use the tweezers to get a little piece of an onion layer and put it onto the slide. B) Add three drops of water to the layer and apply the cover slip at a 45° angle. C) Move the slide onto the stage and adjust the fine and course adjustment knob until the cells are visible. For the onion cell with methylene blue just do the same thing, instead of adding three drops of water just add three drops of methylene blue.

Observations: For the low power lens for the cheek cell. I only saw that one cell at a field of view of 0. I saw about 4 cells that were also black and had a circular shape and were solid and not sting like. and a few string like cells that were as thin as a pin.005mm because I was comparing it to the field of view of the lens. which is 4. The field of view for these cells was about 0. Also the previous estimate for the field of view has changed to a fraction of it. because after looking at it through the medium lens I changed my estimate to 0. . It was the size of a tiny marble but it had a weird shape. I saw miniscule cells that were the size of pinheads. But the cell may be even smaller than our estimate for its size.00mm. So I basically saw some of the same cells in the low power lens but they were more magnified.004 mm or 4 micrometers. They looked like small black beads. I believe that it was a nucleus because it’s one of the largest organelles in an animal cell.6 micrometers. These cells were black and were not in motion. Medium power for Water Cheek Cell: On the medium power lens I saw a few of the same cells that I saw on low power. High power for Water Cheek Cell: For the high power lens I saw a cell that was transparent but had a small black dot that was the size of a pen’s ball tip.0016mm or 1.

The colour for this cell was transparent and sort of green. . The larger cell is the size of a bean and the smaller cell is basically half the size of the larger cell.Low power for Methylene Blue for Cheek Cells: For the low power with methylene blue I saw about 3-5 cells that were moving slightly. The field of view for the largest cell is about 25 micrometers. Other than that I saw external bubbles. Their width was the size of a pin and it was black. The field of view for the cell was about 50 micrometers. Medium for Methylene Blue: For Medium I saw two cells that were the size of a tiny bubble. The field of view is still 50 micrometers. They were string like and bunched up. Also they were in motion but moved very slowly. I also saw a couple of smaller cells that were the size of pinheads. The cells were black and a bit transparent. High for Methylene Blue: For High power I found two cells that I believe are the same cells for the medium. but they are magnified and the quantity is still the same. These cells were also string like as well. I also saw two air sockets. The size of the bunched up cells is still about 50 micrometers and it was the size of a tiny rock. For this magnification the cell is a lot more transparent and I did not see any organelles inside of the cell. Onion Cell Low Power for Onion Cell: I saw a large cell that is the size of a dandelion pedal.

I also saw a few bubbles. The field of view is about 30 micrometers. Also when you adjust the fine adjustment knob it appears to look like its 3D. The cell was green and a bit transparent and due to that I was able to spot about three organelles.05mm. The cell had something that looked like a vacuole or a nucleus inside of it because the nucleus and vacuole are one of the largest organelles in the plant cell. The colour was also transparent and also sort of green just like the colour for the low power lens. High Power for Onion Cell: For the high power lens I saw a small cell that looked like a small hook. The field of view is around 15 micrometers. The field of view is about 0. The field of view is still about 15 micrometers.Medium Power for Methylene Onion Cell: For the medium power lens I saw a cell that was the size and the shape of a splinter. But I still think it is a nucleus due to its shape because a vacuoles shape is not usually circular while a nucleus’s is. Medium Power for Methylene Blue Onion Cell: For the plant cell on medium power I saw about six cells on which you could identify the cell wall. Low Power for Methylene Blue Onion Cell: For the onion cell on the low power lens I saw a cell that also looked like a small transparent hook. The cell also had a little bit of turquoise on it due to the methylene blue. .

It was also to determine what you can see better with under a microscope. Finally my observations state that for the onion cell with methylene blue. I believe that my hypothesis was correct because I was able to see the plant and animal cell more enhanced and with more clarity with methylene blue. as my hypothesis was that it would be easier to spot the cells with methylene blue rather than with water. my observations state that for the onion cell on high power I was able to see the cells and organelles better with the methylene blue than the water. . So my conclusion does confirm my hypothesis.04mm. The cells that were clearly visible had a green glow and you could spot a few organelles inside of them. It was also saying the animal cell will not have an actual shape and the plant cell will have a rectangular shape. Conclusion: The purpose of this experiment is to determine the difference between a plant and animal cell while looking at them under a microscope. The field of view was about 0. My observations were also able to state that the animal cell did have a circular shape and the plant cell did have a rectangular shape. rather than the water.High Power for Methylene Blue Onion Cell: I saw about seven cells but four of them were cut off due to the field of view being too small. methylene blue or water. My hypotheses was stating that I think I will be able to see better with methylene blue because it dyes the cells and water does not. Furthermore. Also the cells did have a rectangular shape. I was able to see the plant cell and its rectangular shape and the animal cell with its circular shape. Also the clarity for this cell was exceptional.

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