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Antimicrobial Properties of Plant

Antimicrobial Properties of Plant

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Published by: matrix93 on Dec 07, 2010
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12/07/2010

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PLANTS

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PI<ACTICAL - THE A.NTIMICROBL\L PROPER1'lFdL()F

AIl\lI: To investigate the antimicrobial properties of plants.

Bi\CKGROUND
Plants are susceptible to infection by ,bacteria and fungi. Several plants, including garlic, 1'!!il1t, nion, oregano, cinnamon and cumin are known to destroy or inhibit o the growth of certain bacteria. A plant with this property is described as antimicrobial. Chemicals in the cel1s of these plants are toxic to bacteria or interfere with their metabolism in some way.

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In this activity you will investigate whether several plants contain antimicrobial chemicals and how effective they are in looking at the growth of bacteria on agar plates.

HYPOTHESIS (suggest with reasons what you might expect to observe on the plates. Use scientific knowledge to suppOliyour prediction).

EQUIP1VIENT
(1,

,

Agar plate with bacteria

plant material
sterile pipette tape

pestle and mortar
paper discs marker pen

* 10cm3 industrial a1cohol
Ij;

Sterile petri dish

sterile forceps

1$

Incubator set at 25(\C

test-tube

filter paper

hole punch

Obtain a plant extract by cnlshing 3g of garlic withJ.tjb1ne sell. RISK ASSESSMENT (a) Keep alcohol (inflammable) away from naked flames.and shake periodically for 10 minutes. . . Repeat the above steps with the other extracts. Decide on a suitable 'control'..Ocm3 of industrial a1coholusing a pestle and mortar. Cc)Use aseptic techniques. .ke'7 . Use sterile forceps to place the test discs onto the bacterial plate with the control (there should be 3 garlic discs and the control per petri dish). 4. 5. 8.-3S~ l0 ~wr1) of ( r)lurfy fUJ? . RELIABILITY AND ACCURACY OF RESULTS (how will you ensure that the results are accurate and reliable? How will you ensure that measurements are precise. 3.. 9.g(:J~[l1t2o1rfJ1) <>jlc. Pour the extract into a test-tube. Pipette 0. Do not tape all round as this will cause anaerobic bacteria to grow as well as stopping the grm¥1:hof aerobic bacteria. (d) Only dispose of used petri dishes after they have been autoclaved. 2. 6. -.!(JC~ "- 1-4. Incubate the plates for 24 hours at 25°C. . (b) Do not open petri dishes containing growing microorganisms. Let the paper discs dry for 10 minutes on open sterile petri dishes. 7.Jrf?€'rJ/11-M1JJ $ 4t'k t=rs .1(0 -Yb1>1ecim10(~+O/c"fIJ-ff.1% of the extract onto a sterile filter paper disc (cut out using a hole punch).JVIETHOD 1. Closethe petri dish and tape down. accurate and repeatable?).

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