This action might not be possible to undo. Are you sure you want to continue?
Connection Between Genes and Proteins 1. Explain why dwarf peas have shorter stems than tall varieties. Dwarf peas are shorter than tall varieties because they lack gibberellins, the growth hormones which normally stimulate the stem elongation. Their failure to make gibberellins is due their lack of the enzyme required for its synthesis. This protein is missing due to a malfunction of the gene coding for it.
2. Explain the reasoning that led Archibald Garrod to first suggest that genes dictate
phenotypes through enzymes. Archibald Garrod was the first to suggest that genes dictate phenotypes through the proteins that catalyze specific chemical reactions in the cell... enzymes. His ideas were based upon inherited diseases which caused an inability to produce certain enzymes, particularly alkaptonuria. This condition causes urine to turn black to due the presence of a chemical which turns dark when exposed to air. Garrod believed that most people have enzymes that break down alkapton. Many decades later, research was conducted that supported his hypothesis. It was confirmed that genes dictate specific enzyme production.
3. Describe Beadle and Tatum’s experiments with Neurospora and explain the contribution they
made to our understanding of how genes control metabolism. Beadle & Edward Tatum’s experiments involved assailing the bread mold, Neurospora with X-rays. Then, they searched among the survivors to find mutants whose nutritional needs differed from the norm. These mutants could not live on the minimal medium given to normal Neurospora. They couldn’t not synthesis important molecules from the nutrients given. Once given complete growth medium, however, the mutants could survive. Beadle & Tatum tried to characterize the specific metabolic defects by growing them on various complete mediums in different vials. The supplement added that allowed for growth would indicate the defect. Then, they went on to determine the mutants’ three classes based on genetic crosses. Each mutant was found to be defective in only 1 gene. Beadle & Tatum were thus able to demonstrate the relationship between enzymes and genes. In addition, their experiment was strong support for their hypothesis: one gene, one enzyme, which stated that a gene’s function is to dictate a specific enzyme’s production.
4. Distinguish between the “one gene–one enzyme” hypothesis and the “one gene–one
polypeptide” hypothesis and explain why the original hypothesis was changed. The “one gene—one enzyme” hypothesis was altered because genes code for proteins, and not all proteins are enzymes. The hypothesis of “one gene—one protein” was then altered again when it was discovered through later research that proteins are composed of several polypeptides, and that each polypeptide has it’s own gene. Thus, the hypothesis is now “one gene—one gene polypeptide.”
5. Explain how RNA differs from DNA.
While DNA and RNA are chemically similar, the former has deoxyribose and the latter ribose. In addition, for nitrogenous bases, thymine in DNA is replaced by uracil in RNA. RNA is the nucleic acid which links DNA and protein synthesis, as genes cannot build proteins directly. RNA molecules are always single stranded.
6. Briefly explain how information flows from gene to protein.
Information in DNA is in the form of specific sequences of nucleotides, or genes. Transcription occurs, synthesis a copy of the DNA, called messenger RNA (mRNA). DNA and mRNA contains the same information. Protein synthesis is then directed during translation, when polypeptides are produced using the direction from mRNA.
Nirenberg knew that the one amino acid produced would have been coded by the codon containing the base repeated three times. Explain the evolutionary significance of a nearly universal genetic code. Describe the promoter. It is a reminder of the kinship of all life on Earth. Compare where transcription and translation occur in prokaryotes and in eukaryotes. Explain the early techniques used to identify what amino acids are specified by the triplets UUU. Methionine indicates the start of translation. In prokaryotes. the signal is given to end transcription. By creating an artificial mRNA molecule made only of the specific repeated base. 14. a special sequence. 12. Translation is the synthesis of a polypeptide under the direction of the mRNA from transcription. 8. and CCC were discovered by Marshall Nirenberg. RNA polymerase recognizes where transcription begins by detecting the promoter’s location. The genetic code is redundant. 10. no codons can specify two amino acids. This means that in lab research. Explain how RNA polymerase recognizes where transcription should begin. Explain what it means to say that the genetic code is redundant and unambiguous. all codons must be read in groups of three nucleotides. mRNA produced by transcription is immediately translated. GGG. Codons specify the placement of amino acids along a polypeptide. and termination. and CCC. the terminator. but unambiguous. genes can be transcribed and translated from one species to another. elongation. The series of codons. The mRNA transcribed from DNA forms triplets called codons. and the transcription unit. Explain how RNA is modified after transcription in eukaryotic cells. must be read in the correct frame for the correct linear sequence of amino acids. including the three major steps of initiation. The codons that do code for the same amino acid tend to differ only in their third base. from the start. 9. Elongation is when the codons of a gene are copied. Bacteria can thus synthesize specific human proteins after the appropriate genes are inserted. The end product is messenger RNA. Explain the significance of the reading frame during translation. Explain the general process of transcription. 11. GGG. In addition. meaning it is shared by organisms ranging from bacteria to plants to animals. The early technique for identifying the amino acids synthesized by the base triplets UUU. Define codon and explain the relationship between the linear sequence of codons on mRNA and the linear sequence of amino acids in a polypeptide. polymerase eventually falls off DNA. Polypeptides begin with methionine when they are synthesized because that amino acid is a promoter. 16. Transcription is RNA synthesis under DNA’s direction. 13. the genetic code shows that all living things arose from a common ancestor. meaning that while several codons can be for the same amino acid. 17. . prokaryotes have sequences that signal the end of transcription. With eukaryotes. In terms of evolutionary significance. which is generally referred to as mRNA. RNA polymerase recognizes a promoter which begins the process. Transcribing produces the transcription unit. where transcription actually begins.7. initiating transcription. AAA. The transcription process is then ended through termination. or codon that initiates the process. At the terminator. On the other hand. The genetic code is almost universal. RNA polymerase then attaches to the promoter. The general process of transcription includes initiation. The Synthesis and Processing of RNA 15. The reading frame is significant during translation because the genetic code’s message will not be correct if the starting point is not right. AAA. The nuclear envelope separates the two steps. Distinguish between transcription and translation. eukaryotes much process the mRNA before translation can occur. Explain why polypeptides begin with methionine when they are synthesized. Then. however.
leaving an end with a head and another with a tail. and two ribosomal sub-units. This may involve adding sugars. Introns are non-coded segments of nucleotides. It is used repeatedly in the cytoplasm as it picks up its designated amino acid in the cytosol. and energy sources are needed for each stage. 22. Each tRNA is connected to the correct amino acid by aminoacyl-tRNA synthase. Both ends are altered.When RNA is processed after transcription. Describe the process of translation (including initiation. Termination occurs when one of the three stop codons reaches the A site. Describe the functional and evolutionary significance of introns. and are evolutionary significance as they protect the mRNA from hydrolyzing agents in the cytoplasm. 21. Polypeptides must be modified with posttranslational modifications before becoming fully functional proteins. Explain how tRNA is joined to the appropriate amino acid. Define and explain the role of ribozymes. Ribosomes require less than minutes to convert an averaged sized mRNA into a polypeptide. This in turn determines the secondary & tertiary structure. Describe two properties of RNA that allow it to perform so many different functions. tRNA with the first amino acid. it is able to bind with A or G. elongation. but synthesize different kinds of proteins. A major difference is that prokaryotes can transcribe and translate the same gene simultaneously. Describe the structure and functions of ribosomes. The discovery of ribozymes rendered the statement that “All biological catalysts are proteins” obsolete. It deposits the amino acid at the ribosome and returns to the cytosol to pick up another amino acid. The wobble is the third position at the base of an anti-codon. is transcribed from DNA templates. Certain animo acids may be removed. Polyribosomes are multiple ribosomes that may trail along mRNA. Prokaryotes and Eukaryotes have different ribosomes and how transcription is terminated. AminoacyltRNA synthase has an active site for a specific tRNA and amino acid combination. 23. Explain the significance of wobble. Some interior parts are also removed and the rest are spliced together. like other RNA molecules. and termination. 18. Free and bound ribosomes have the same structure. elongation. 25. Elongation consists of a series of three step cycles as each amino acid is added. and termination) and explain which enzymes. Initiation takes an mRNA. protein factors. or etc. Compare protein synthesis in prokaryotes and in eukaryotes. lipids. 19. 26. The sub-units are made up of proteins and rRNA. translation can also be separated into the same three stages: initiation. Ribosomes help with the specific pairing of anti-codons in tRNA with mRNA codons during protein synthesis. tRNA. The primary structure of a protein is determined by its coiling and folding into 3-D shape during/after synthesis. A ribosome is composed of a large and small sub-unit. Describe the significance of polyribosomes. Then. Ribozymes are RNA that works like enzymes. If U on the anti-codon is in this position. resulting with the new protein to be able to diffuse to its operating site . The ability of RNA to form hydrogen bonds contributes to its many functions. It can bond with the nucleic acids of both DNA and RNA 29. TRNA is consisted of about 80 nucleotides that folds back on itself to create a three dimensional figure. two or more polypeptides may unite to form one protein. Like transcription. The Synthesis of Protein 20. Explain what determines the primary structure of a protein and describe how a polypeptide must be modified before it becomes fully functional. which is the most abundant RNA in the cell. 28. Describe what determines whether a ribosome will be free in the cytosol or attached to the rough endoplasmic reticulum. 27. Describe the structure and functions of tRNA. 24.
This however has further evolved into the current definition of a gene: A gene is a region of DNA whose final product is either a polypeptide or an RNA molecule. outside. Describe the historical evolution of the concept of a gene. Physical mutagens may be high-energy radiation. This has a disastrous effect on the protein. which then forms a frameshift mutation. 32. Base-pair substitution is when the nucleotide pair is replaced with a pair of complimentary nucleotides. 31. Chemical mutagens may be base analogues that substitute DNA.quickly.5 opposite (anticodon: a specialized base triplet on one end of a tRNA molecule that recognizes a particular complementary codon on an mRNA molecule) exo. Insertions are additions the nucleotide pairs. interfere with DNA replication by going in DNA and distorting the double helix shape.5 change. but pair incorrectly. This evolved into the one gene – one polypeptide definition. Describe several examples of mutagens and explain how they cause mutations. Define point mutations. -gen 5 producing (mutagen: a physical or chemical agent that causes mutations) poly. Mutagens are chemical or physical agents that interact with DNA to cause a mutation.5 many (polyA tail: the modified end of the 39 end of an mRNA molecule consisting of the addition of some 50 to 250 adenine nucleotides) trans. without (exon: a coding region of a eukaryotic gene that is expressed) intro. The Mendelian view of a gene sees it as a unit of inheritance that affects phenotype.5 across. Concept Map Words: 59 cap A site alternative RNA splicing aminoacyl-tRNA synthetase anticodon base-pair substitution codon deletion domain E site exon frameshift mutation insertion intron messenger RNA (mRNA) missense mutation mutagen mutation nonsense mutation one gene–one polypeptide hypothesis P site point mutation poly-A tail polyribosome (polysome) primary transcript promoter reading frame ribosomal RNA (rRNA) ribosome ribozyme RNA polymerase RNA processing RNA splicing signal peptide signal-recognition particle (SRP) spliceosome TATA box template strand terminator transcription transcription factor transcription initiation complex transcription unit transfer RNA (tRNA) translation triplet code wobble Memorize the Word Roots anti. Distinguish between base-pair substitutions and base-pair insertions. which is useful in certain context.5 out. intervening sequence within a eukaryotic gene) muta. Give examples of each and note the significance of such changes. 30. This may have little or no impact on protein function. unless it occurs in a multiple of three. some change the bases to change their pairing properties. such as X-rays and ultraviolet light. Point mutation is a chemical change in a single base pair of a gene. -script 5 write (transcription: the synthesis of RNA on a DNA template) .5 within (intron: a noncoding.
This action might not be possible to undo. Are you sure you want to continue?
We've moved you to where you read on your other device.
Get the full title to continue listening from where you left off, or restart the preview.