St. Croix Estuary Project Inc.

March, 2003

Monitoring Kit
The St. Croix Estuary
Objective:

The purpose of this kit is to provide a set of fixed sampling sites and sampling protocols that can be used over the long term to quickly and
efficiently assess the health of the St. Croix River Estuary. Detailed field studies should be conducted at 3 to 5 year intervals or when annual
assessments show there are problems or changes that need more detailed study.

Protocols:

There are three components to the field survey:

1. Chemistry and Bacteriology - Water and sediment samples will be taken for ammonia, nitrite, nitrate, phosphate, salinity, conductivity,
temperature, pH, and redox, dissolved oxygen, total coliforms, and E. coli.

2. Intertidal Biota - The presence or absence of indicator species from the intertidal area will be surveyed.

3. Subtidal Biota and Sediments - The presence or absence of indicator species and bottom condition will be determined by remote video or
diver generated video.

Field teams of not less than 2 trained staff and/or volunteers will be responsible for each component.

Timing:

The preferred time for sampling is August and September, but no later than October 31.

Equipment:

a) Subtidal Sampling - camcorder, underwater camera, AC source, computer with movie application. Diver, boat, and Underwater camera or
remote underwate camera used from a kayak or canoe.
b) Intertidal Biota - Boots, Clipboard and data sheets.
c) Sediments & Water -ORP/pH/temperature meter; test Strips, bottles and kits for: sulphides, salnity, dissolved oxygen, phosphates, ammo-
nia, nitrite, nitrate, bacteria; gloves and data sheets.

3. Study Zones

4. Study Sites
Field Data Sheet
 ZONE J
ZONE J - Buchanan Hill to Calais Golf
Club - Station Locations
Site Data Cove ZONE J - Station 1
2002 2003 2004
Location: This station is located at “The Cove” above the Inter-
Total Coliform >2000 >2000
national Bridge in St. Stephen.
E.coli 800 885
Nitrate Timing: This station should be visited between 2 hours to low
Nitrite water and 2 hours after low water.
Phosphates
Ammonia Access: Park in the Parking area just south of the strip park.
Temperature This is probably public parking, but permission should be
sought from the residents of the adjacent house. Alternate Park-
pH
ing exists in the Park at the top of Bucanan Hill. Access to the
Redox shore is at the Kiosk overlooking the Cove.

2005 2006 2007 Sampling Requirements

Total Coliform >2000 a) Biota
E.coli 885 b) Sediments
Nitrate 0 c) Water
Nitrite 0
Phosphates 15
Ammonia
Temperature 16.1
pH 5.95
Redox 128

2008 2009 2010

Total Coliform
E.coli
Nitrate
Nitrite
Phosphates
Ammonia
Temperature
pH
Redox
ZONE J - Station 2A Site Data Clarke
2002 2003 2004
Location: This station is located behind the Clarke Building on
Total Coliform > 20 000 > 20 000
Milltown Blvd.
Timing: This location should be visited between 2 hours to low water E.coli > 20 000 16 520
and 2 hours after low water. Nitrate
Nitrite
Access: Park in the Parking Lot behind the Clarke Building. Phosphates
Ammonia
Sampling Requirements
Temperature
a) Biota
pH
b) Sediments
c) Water Redox

2005 2006 2007

Total Coliform >20 000
E.coli >20 000
Nitrate 0
Nitrite 0
Phosphates 30
Ammonia
Temperature 23.2
pH 5.1
Redox 95

2008 2009 2010

Total Coliform
E.coli
Nitrate
Nitrite
Phosphates
Ammonia
Temperature
pH
Redox
ZONE I
ZONE I - Station 5 Site Data
2002 2003 2004
Location: This station is located at The Narrows. Take the Donald
Total Coliform 260
Road off the Ledge Road.
Timing: This location should be visited between 2 hours to low water E.coli 10
and 2 hours after low water. Nitrate 0
Nitrite 0.1
Access: Park in the area. Phosphates 5
Sampling Requirements Ammonia 0.25
a) Biota Temperature
b) Sediments
pH
c) Water
Redox

2005 2006 2007

Total Coliform >2000
E.coli 87
Nitrate 0
Nitrite 0
Phosphates 30
Ammonia
Temperature 17.9
pH 6.4
Redox 133

2008 2009 2010

Total Coliform
E.coli
Nitrate
Nitrite
Phosphates
Ammonia
Temperature
pH
Redox
ZONE H
ZONE H - Station 6 Site Data
2002 2003 2004
Location: This station is located at the Ledge Loop.
Total Coliform
Timing: This location should be visited between 2 hours to low water
and 2 hours after low water. E.coli
Nitrate 2
Access: Park in the area. Nitrite 0.15
Sampling Requirements Phosphates 5
a) Biota Ammonia 0.5
b) Sediments Temperature 9.6
c) Water
pH 7.53
Redox 154.1

2005 2006 2007

Total Coliform >2000
E.coli 782
Nitrate 0
Nitrite 0
Phosphates 30
Ammonia
Temperature 17.8
pH 8.2
Redox 165

2008 2009 2010

Total Coliform
E.coli
Nitrate
Nitrite
Phosphates
Ammonia
Temperature
pH
Redox
ZONE G
ZONE G - Station 8 Site Data
2002 2003 2004
Location: This station is located at the beach by the Oak Bay Camp-
Total Coliform 100
ground.
Timing: This location should be visited between 2 hours to low water E.coli >10
and 2 hours after low water. Nitrate 0
Nitrite 0.7
Access: Park in the area. Phosphates 2
Sampling Requirements Ammonia 0.15
a) Biota Temperature 9.5
b) Sediments
pH 7.83
c) Water
Redox 216.8

2005 2006 2007

Total Coliform 99
E.coli 1
Nitrate 0
Nitrite 0
Phosphates 30
Ammonia
Temperature 22
pH 7.8
Redox 145

2008 2009 2010

Total Coliform
E.coli
Nitrate
Nitrite
Phosphates
Ammonia
Temperature
pH
Redox
ZONE E
ZONE E - Station 10 Site Data
2002 2003 2004
Location: This station is located at the Ganong Nature Park at Todd’s
Total Coliform 60
Point.
Timing: This location should be visited between 2 hours to low water E.coli <10
and 2 hours after low water. Nitrate 0.5
Nitrite 0
Access: Park in the area. Phosphates 5
Sampling Requirements Ammonia 0
a) Biota Temperature 11.6
b) Sediments
pH 7.99
c) Water
Redox 225.2

2005 2006 2007

Total Coliform 192
E.coli 53
Nitrate 0
Nitrite 0
Phosphates 15
Ammonia
Temperature 18.1
pH 8.3
Redox 185

2008 2009 2010

Total Coliform
E.coli
Nitrate
Nitrite
Phosphates
Ammonia
Temperature
pH
Redox
ZONE D
ZONE D - Station 12 Site Data
2002 2003 2004
Location: This station is located at the Island View Campground at
Total Coliform
Sandy Point.
Timing: This location should be visited between 2 hours to low water E.coli
and 2 hours after low water. Nitrate 0.5
Nitrite 0.12
Access: Park in the area. Phosphates 2
Sampling Requirements Ammonia 0.12
a) Biota Temperature 10.8
b) Sediments
pH 7.06
c) Water
Redox 244.1

2005 2006 2007

Total Coliform 271
E.coli 22
Nitrate 0
Nitrite 0
Phosphates 15
Ammonia
Temperature 15
pH 8.1
Redox 148

2008 2009 2010

Total Coliform
E.coli
Nitrate
Nitrite
Phosphates
Ammonia
Temperature
pH
Redox
ZONE C
ZONE C - Station 13 Site Data
2002 2003 2004
Location: This station is located at the Garcelon’s in Johnson Cove.
Total Coliform 50
Timing: This location should be visited between 2 hours to low water
and 2 hours after low water. E.coli >10
Nitrate
Access: Park in the area. Nitrite
Sampling Requirements Phosphates
a) Biota Ammonia
b) Sediments Temperature
c) Water
pH
Redox

2005 2006 2007

Total Coliform 504
E.coli 0
Nitrate 0
Nitrite 0
Phosphates 30
Ammonia
Temperature 16.6
pH 7.7
Redox 225

2008 2009 2010

Total Coliform
E.coli
Nitrate
Nitrite
Phosphates
Ammonia
Temperature
pH
Redox
ZONE B
ZONE B - Station 14 Site Data
2002 2003 2004
Location: This station is located at the furthest end of the main build-
Total Coliform 120 504
ing on your right at the St. Andrews Biological Station on Brandy
Cove Rd. E.coli >10 11
Timing: This location should be visited between 2 hours to low water Nitrate 0.5
and 2 hours after low water. Nitrite 0.07
Phosphates 0
Access: Park in the area designated for parking Ammonia 0.2
Sampling Requirements Temperature 12.6
a) Biota
pH 7.78
b) Sediments
c) Water Redox -9.1

2005 2006 2007

Total Coliform 1090
E.coli 1
Nitrate 0
Nitrite 0
Phosphates 30
Ammonia
Temperature 15.9
pH 7.9
Redox 203

2008 2009 2010

Total Coliform
E.coli
Nitrate
Nitrite
Phosphates
Ammonia
Temperature
pH
Redox
ZONE A
ZONE A - Station 15 Site Data
2002 2003 2004
Location: This station is located at the furthest point east in St An-
Total Coliform 504
drews. Enter on the main road of St Andrews and drive until you are
adjacent to the Kiwanis Campground. E.coli 11
Nitrate
Timing: This location should be visited between 2 hours to low water Nitrite
and 2 hours after low water. Phosphates
Ammonia
Access: There is available parking on the side of the road. Temperature
pH
Sampling Requirements
a) Biota Redox
b) Sediments
c) Water 2005 2006 2007
Total Coliform >2000
E.coli 53
Nitrate 0
Nitrite 0
Phosphates 30
Ammonia
Temperature 26
pH 7.5
Redox 145

2008 2009 2010

Total Coliform
E.coli
Nitrate
Nitrite
Phosphates
Ammonia
Temperature
pH
Redox