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Nanotechnology 101

Nanotechnology 101

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Published by Mohammad Nizam

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Published by: Mohammad Nizam on Feb 18, 2011
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01/31/2013

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The transmission electron Microscope (TEM) was the first type of
electronmicroscopetobedeveloped.ItwasdevelopedbyMaxKnolland
Ernst Ruska in Germany in 1931. The transmission electron microscope
(TEM) operates on the same basic principles as the light microscope
but uses electrons instead of light. As mentioned earlier, what you can
see with a light microscope is limited by the wavelength of light. TEMs
useelectronsasa“lightsource”andtheirmuchlowerwavelengthmakes
it possible to get a resolution a thousand times better than with a light
microscope. The enlarged version of the imageof the specimen appears
on a fluorescent screen or in a layer of photographic film.
You can see objects to the order of .2 nanometers. For example, you
canobserveandstudysmalldetailsinthecellorotherdifferentmaterials
down to near atomic levels. The microscope’s high magnification range
and resolution has made the TEM a valuable tool in medical, biological,
and materials research.
Transmission electron microscopy has had an important impact on
the knowledge and understanding of viruses and bacteria. The improve-
ment in resolution provided by electron microscopy has allowed vi-
sualization of viruses as the causes of transmissible infectious disease.
Researchers are continuing the use of electron microscopy in the inves-
tigation of such pathogens as SARS and the human monkeypox virus.
SARS is a severe acute respiratory disease in humans which is caused by
the SARS virus. Monkeypox is a rare smallpox-like disease that is most
common in the rain forests of central and West Africa.

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