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rr422304-biophysics-of-macromolecules

rr422304-biophysics-of-macromolecules

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Published by: SRINIVASA RAO GANTA on Aug 26, 2008
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05/09/2014

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Code No: RR422304

Set No. 1

IV B.Tech II Semester Supplimentary Examinations, May 2008 BIOPHYSICS OF MACROMOLECULES (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. (a) Write about primary structure of different types of macromolecules. (b) Write about the geometry of a simple helix. [8+8]

2. (a) What are the advantages of smaller systems as a model biophysical studies. (b) Explain about structure of the functional site of a macromolecule in biophysical studies. [10+6] 3. Write about the IUPAC-IUB sheme for rotation angles that are encountered in the three-dimensional conformation of macromolecules. [16] 4. Write short notes on (a) Hydrogen bonding (b) Hydrophobic interactions (c) Ionic interactions (d) Disulphide bonds [4+4+4+4]

5. (a) Write about the backbone rotational isomers for a polynucleotide chain. (b) Write about different puckered conformers of deoxy ribose ring in a polynucleotide chain. [8+8] 6. Write a note on the following: (a) Steady State Kinetics (b) Multiple Substrate Reactions (c) Ligand Interactions. 7. Write a note on the following: (a) Sedimentation Velocity (b) Gradient centrifugation (c) Viscometry. [16] [16]

8. What is Micrososcopy? Explain how small particles visualized with the help of a Microscope? [16] ⋆⋆⋆⋆⋆ 1 of 1

Code No: RR422304

Set No. 2

IV B.Tech II Semester Supplimentary Examinations, May 2008 BIOPHYSICS OF MACROMOLECULES (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. (a) Write about the size and complexity of biopolymers with specific example for each class. (b) Explain the different secondary structural forces involved in protein conformation. [8+8] 2. Write about the structural and functional components involved in the study of biopolymers. [16] 3. (a) What is the contribution of dipolar interactions to potential energy producing rotations? (b) Write about various bond lengths and bond angles that characterize the polypeptide chain backbone. [10+6] 4. Write short notes on (a) Hydrogen bonding (b) Hydrophobic interactions (c) Ionic interactions (d) Disulphide bonds [4+4+4+4]

5. Write the general characteristics of polynucleotide chain , types of residues and the bonds involved. [16] 6. List at least two reasons why almost all enzyme-catalyzed reactions show pH optimum and illustrate the relaxation technique for a first order reaction? [16] 7. (a) Explain the measurement of molecular mass of hemoglobin in an aqueous solution may show that the solution is monodisperse or polydisperse, depending on experimental conditions? (b) A poly disperse solution has the following distributions? No. of Molecules Molar Mass (g mol−1 ) 10 25000 7 17000 24 31000 16 49000 Calculate both Mn and Mw and the polydispersity of solution. 8. Write a note on the following: 1 of 2

[16]

Code No: RR422304 (a) Phase contrast Microscope (b) Flourescence Microscope (c) Polarised Light Microscope. ⋆⋆⋆⋆⋆

Set No. 2
[16]

2 of 2

Code No: RR422304

Set No. 3

IV B.Tech II Semester Supplimentary Examinations, May 2008 BIOPHYSICS OF MACROMOLECULES (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. What are the different parameters considered in the study of function of macromolecules in biophysical studies? [16] 2. Write the three dimensional arrangement of residues in biopolymers and the different forces involved in the conformation stabilization. [16] 3. Write about basic principle of steric contour diagrams and explain it with a suitable example. [16] 4. Write short notes on (a) Hydrogen bonding (b) Hydrophobic interactions (c) Ionic interactions (d) Disulphide bonds [4+4+4+4]

5. (a) Explain different rotational angles and steric hindrances for a polyribonucleotide chain. (b) Explain about ribose puckering in a polyribonucleotide chain. [10+6]

6. Explain? Why the presences of small amounts competitive inhibitors often act as activators to allosteric enzymes? [16] 7. Write a note on the following: (a) Gel Filtration (b) Electrophoresis (c) Optical Rotation. 8. Describe the various types of visual tools of Microscopy? ⋆⋆⋆⋆⋆ [16] [16]

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Code No: RR422304

Set No. 4

IV B.Tech II Semester Supplimentary Examinations, May 2008 BIOPHYSICS OF MACROMOLECULES (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Write about the arrangement of residues in the order of complexity for a biopolymer with subunits along with the physical and chemical forces involved. [16] 2. (a) Write a general scheme for multicomponent analysis in biophysical studies. (b) What are the basic strategies used to compare two systems that are almost identical. [8+8] 3. What are the different forces that determine the nucleic acid conformation? [16] 4. (a) Explain about a contour diagram and write its significance in a polypeptide study. (b) Write the significance of potential energy in protein conformation. 5. Write about structural differences between RNA and DNA. [10+6] [16]

6. Define Non - competitive inhibition and calculate the concentration of a non competitive inhibitor needed to yield a 90% inhibition of an enzyme catalyzed reaction? (where Ki = 2.9 × 10−4 M ) [16] 7. Write a note on the following: (a) Gel Filtration (b) Electrophoresis (c) Optical Rotation. [16]

8. What is Micrososcopy? Explain how small particles visualized with the help of a Microscope? [16] ⋆⋆⋆⋆⋆

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