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TroubleshooTing guide

Columns (capillary to preparative) SEC Columns: Aqueous (GFC) and non-Aqueous (GPC) n Amino Acid Analysis n HPLC Specialty Columns for Analysis of: n Basic, acidic and amphoteric drugs n High/Low pH separations (pH 1-12) n Proteins/Peptides by reversed phase n Biopolymers - Proteins and Nucleic Acids by GFC/SEC n Synthetic polymers n Foods and Beverages n Environmental Samples n Drugs in biological fluids n HPLC Bulk Media n HPLC Accessories such as: n Sample and Solvent Filters n SecurityGuard Column Protection n Syringe Filters n Syringes and Vials n Column Heater / Column Chiller-Heater n HPLC Injection Valves n Tubings and Fittings n Solvent Degassers n Column Selectors n Fluid Processors n GC Columns n SPE Tubes and 96-Well plates
n n Application n Outstanding n HPLC

Development and Validation Support Technical Service

....... 2 Leaks ................................................................. While every attempt has been made to ensure the accuracy of the information contained in this guide.......... 4 Problems with the Chromatogram ................................. 15 Key Problem Areas and Preventive Maintenance............ 1 Abnormal Pressure .......................................... Introduction.................................... 17 SecurityGuard Universal HPLC Guard Cartridge System............................. 20 © 2008 Phenomenex. IV..................... VII.................................. Sight....... II................................ Inc..................................... III.............................. VI.. 19 Phenex Syringe Filters .............. ........TABLE OF CONTENTS I..... All rights reserved............... V....... No part of this booklet may be copied without prior written permission from Phenomenex. We welcome any additions or corrections for incorporation into future editions................. 14 Problems Detected by Smell... 6 Problems with the Injector ...................... Inc... or Sound ............ USA... Phenomenex assumes no responsibility for its use...............

replacing pump seals at regular intervals should eliminate pump-seal failure and its associated problems. Wiley. Snyder. J. Phenomenex Order No. Phenomenex Order No. Dolan and L. Troubleshooting LC Systems. • Phenomenex has experienced technical consultants who can assist you with almost any problem. NY (1979). Maintaining and Troubleshooting HPLC Systems . NJ (1989).A User’s Guide. Most LC manufacturers offer free technical support to their customers. as well as a complete line of reference books on HPLC.. WHERE TO GET AddITIONAL HELP • The operator’s and service manuals for the instrument should be consulted. • The manufacturer of your instrument can help you. they can be a helpful resource. Kirkland. 1 . and preventive maintenance practices that will reduce their frequency. “Troubleshooting”. faxes or emails.: AA0-1700 D.R. These contain exploded diagrams. Humana Press. troubleshooting procedures for specific models. Runser. and then made a regular part of your laboratory routine. LC/GC Magazine. and sound When you have corrected the problem. Snyder and J.J. Wiley. Introduction to Modern Liquid Chromatography. For example. PREVENTION Many LC problems can be prevented with routine preventive maintenance. sight.I.R. This is a monthly column. This guide is organized by five major categories of symptoms to help you quickly identify the source of the problem(s) you are encountering: • pressure abnormalities • leaks • problems with the chromatogram • injector problems • other problems detected by the senses of smell. Section VII lists the most common problem areas for each LC module.W.W. • Phenomenex offers seminars.: AA0-1717 L. 2nd ed.J. We welcome your phone calls. and part numbers to help you order replacement parts. NY (1981). INTROduCTION LOCATING ANd CORRECTING THE PROBLEM A systematic approach to identifying the problem is the best approach to troubleshooting your HPLC system. • There are a number of reference sources that can give you guidance in problem solving: J. • Other people in the lab may have had experience solving a problem which is giving you trouble. These suggestions should be modified to fit your particular model of LC. record the incident in the system recordbook to help with future problems. Dolan.

no flow POSSIBLE CAuSE SOLuTION 1. Replace column 3. Tighten or replace fittings B. prime pump 6. 2 . Choose the category below that best fits the symptoms that you observe. Column temperature too low 7. Replace piston 5. Flow rate set too high 2. Replace transducer C. Turn on power 2. Removal of end-fittings may void column warranty. Degas solvents. Replace frit* c. Air trapped in pump head 6. a. Steady. Injector blockage 6. Controller setting or failure 4. Repair or replace controller 8. Blocked guard column 9. Raise temperature 7. Faulty meter 2. and follow the suggestions to correct the problem. Verify proper settings b. a. Adjust setting 2. Power off 2. Repair or replace controller 4. Use proper column 5. Broken piston 5. Replace inlet frit if blocked 7. Replenish reservoir b. Controller malfunction 8. No pressure reading. Major leak 1. a. Wash column 4. Replace fuse 3. Clear blockage or replace injector 6. Replace check valve(s) 8. bleed air from pump.II. a. Insufficient mobile phase 7. precipitated buffer 4. ABNORMAL PRESSuRE A change in the operating pressure is a sign that there may be a problem. Backflush column (if permitted) b. Remove/replace guard column 9. A. Improper column 5. Blocked column frit 1. Remove/replace in-line filter 3. Replace meter 2. Improper mobile phase. No pressure reading. Faulty pressure transducer 1. Fuse blown 3. Faulty check valve(s) 8. Blocked in-line filter * Check manufacturer’s column warranty first. Use correct mobile phase b. high pressure POSSIBLE CAuSE SOLuTION 1. flow is normal POSSIBLE CAuSE SOLuTION 1.

Lower temperature 5. a. Controller malfunction 1. Pressure dropping to zero POSSIBLE CAuSE SOLuTION 1. Change degassing methods (use Degassex on-line degasser) 5. Flow set too low 2. Leak in system 6. Insufficient degassing 1. See section D 1. ABNORMAL PRESSuRE (continued) D. See section D H. See sections A and B 1. Degas solvent b. Improper column 4. Steady. Bleed air from pump 2. Pressure dropping. Adjust flow rate 2. See section C 1. Using gradient elution 5. Replace check valve(s) 3. Repair or replace controller E. Pressure cycling is normal due to viscosity changes 3 . Faulty check valve(s) 3. Air in pump 2.II. but not to zero POSSIBLE CAuSE SOLuTION 1. Replace pump seal 4 a. Column temperature too high 5. Pressure climbing POSSIBLE CAuSE SOLuTION 1. See sections A and B G. Locate and correct 6. Pump seal failure 4. See section C F. Degas solvent b. Locate and correct 3. Pressure cycling POSSIBLE CAuSE SOLuTION 1. low pressure POSSIBLE CAuSE SOLuTION 1. Leak in system 3. Use proper column 4.

Repair or replace 5. Tighten fittings (do not overtighten) 3. a. replace 8. A. Leaks at pump POSSIBLE CAuSE SOLuTION 1. Replace purge valve 2. Replace check valve 2. Replace 3. distorted ferrule. Loose fitting 2. take the fitting apart and inspect for damage (e.g. Repair or replace 6. a. Check diaphragms. Loosen and retighten b. a. Check for fitting damage. Loose check valves 1. LEAkS Leaks are usually stopped by tightening or replacing a fitting. Replace mixer 4. Be aware. Tighten valve b. Tighten check valve (do not overtighten) b. a. Tighten 2. or particles on the sealing surface). damaged fittings should be discarded. Pressure transducer failure 6.III. Purge valve * Use fingertight end-fittings to avoid sealing problems and the need for wrenches 4 . Disassemble and clean b. Loose fittings 3. a. Leaky fittings POSSIBLE CAuSE SOLuTION 1. Replace 4. Replace mixer seal b. Use all parts from same brand B. Proportioning valve failure 8. replace if leaky b. Replace 5. a. Mismatched parts 1. Overtightened* fitting 4. Stripped fitting 3. If a fitting leak does not stop when the fitting is tightened a little. Mixer seal failure 4. Replace pulse damper 7. that overtightened metal compression fittings can leak and plastic fingertights can wear out. Pulse damper failure 7. however. Pump seal failure 5. Dirty fitting 5.

Leaky fittings 4. Blocked waste line 5. Replace waste line D. Disassemble. Cell gasket failure 2. Improper frit thickness 1.20 µm 0. rinse ferrule. Loose injection-port seal 4. Blocked flow cell 1. Cracked cell window(s) 3. Replace waste line 5. Replace loop 3.5 µm 2 µm 5 . Column packing in ferrule 3. Replace gasket 2. Rebuild or replace injector 2. Tighten or replace 4. Use proper frit (see chart below) E. Loose endfitting 2. Use correct syringe 5. Waste-line siphoning 6. a. Adjust 4.III. Prevent excessive backpressure b. Rotor seal failure 2. Rebuild or replace FRIT PORE SIzE SELECTION GuIdE When Particle Size of material is: Frit Pore Size should be: 3 . Injector leaks POSSIBLE CAuSE SOLuTION 1. Column leaks POSSIBLE CAuSE SOLuTION 1. Tighten endfitting 2. Improper syringe-needle diameter 5. LEAkS (continued) C.4 µm 5 . reassemble 3. Keep waste line above surface waste 6. Waste-line blockage 1. Blocked loop 3. Replace window(s) 3. Detector leaks POSSIBLE CAuSE SOLuTION 1.

Column void 3. lower pH usually provides more symmetric peaks 5. PROBLEMS WITH THE CHROMATOGRAM Many problems in the LC system show up as changes in the chromatogram. a.2. Decrease sample concentration 4. Replace column 2. Sample reacting with active sites. Remove guard column and attempt analysis. Bad column 1. Split peaks POSSIBLE CAuSE SOLuTION 1.IV. Wrong mobile phase pH. 1. Replace guard continued on next page * Check manufacturer’s column warranty first. Change mobile phase and/or column/selectivity 4. Replace inlet frit* c. Add ion pair reagent or volatile basic modifier b. 6 . Wrong sample solvent 3. a. 5. Contamination on guard or analytical column inlet. C. a. Peak fronting POSSIBLE CAuSE SOLuTION 1. Some of these can be solved by changes in the equipment. Adjust pH. others require modification of the assay procedure. Sample overload 4. Use longer column b. Fill void 3. however. Selecting the proper column type and mobile phase are keys to “good chromatography. Change column 2. See A. Peak tailing POSSIBLE CAuSE SOLuTION 1. Normal Problem PEAk TAILING B. Interfering peak 4. Removal of end-fittings may void column warranty. Low temperature 2. For basic compounds. Blocked frit 1. and A. Use mobile phase for injection solvent 3. Reverse fIush column (if allowed) b.” A. Increase column temperature 2.1.

Distortion of early peaks POSSIBLE CAuSE SOLuTION 1. If problem persists. reversed-phase mode 1. Secondary retention effects. column may be fouled with strongly retained contaminants. Wrong injection solvent 1. Add acetic acid (acidic compounds) 7 . normal-phase mode 2. Distortion of larger peaks POSSIBLE CAuSE SOLuTION 1.IV. See page 19 2. b. Add triethylamine (basic samples) Add acetate (acidic samples). Add salt or buffer (ionic samples) Try a different column. Reduce sample size E. Tailing. If analytical column is obstructed. Split peaks (continued) POSSIBLE CAuSE SOLuTION Normal Problem SPLIT PEAkS if necessary. a. narrower tubing) b. Use appropriate restoration procedure. If problem persists. Extra-column effects 1. inlet is probably plugged. Increased tailing as k’ increases POSSIBLE CAuSE SOLuTION 1. Sample solvent incompatible with mobile phase. Whenever possible. Reduce injection volume b. Change frit or replace column 2. Change solvent. e. reverse and flush. d. Replumb system (shorter. Use smaller volume detector cell G. Add triethylamine (basic compounds) b. early peaks more than later ones POSSIBLE CAuSE SOLuTION 1. Use weaker injection solvent F. a. PROBLEMS WITH THE CHROMATOGRAM (continued) C. D. a. a. Sample overload 1. Secondary retention effects. c. inject samples in mobile phase 2.

IV. Increase run time or gradient slope b. Poor temperature control 2. PROBLEMS WITH THE CHROMATOGRAM (continued) G. Retention time drifts POSSIBLE CAuSE 1. Normal 2. Improper mobile phase 1. Vacancy or ghost peaks 1. Late-eluting peak from previous injection 3. Add triethylamine (basic samples) 2. normal-phase mode H.) 3. Contamination J. Abrupt retention time changes POSSIBLE CAuSE 1. Reduce injection volume 4. Use buffer with pKa equal to pH of mobile phase c. a. Thermostat column 2. Increase flow rate 3. reaction. Secondary retention effects. Flow rate change 2. ion-pair 3. Filter sample SOLuTION 4. Prevent change (evaporation. Check purity of mobile phase b. Only for normal-phase methods which use water-miscible solvents. a. Increased tailing as k’ increases (continued) POSSIBLE CAuSE SOLuTION 2. Set proper mobile phase mixture on controller 8 . Mobile phase changing 3. Use mobile phase as injection solvent c. Use 50-100 mM buffer concentration b. Reset flowrate 2. c. Bleed air from pump 3. a. Try a different LC method 3. Poor column equilibration 1. Add water (poly-functional compounds). etc. See page 19 SOLuTION I. Extra peaks POSSIBLE CAuSE 1. Secondary retention effects. Acidic or basic peaks tail POSSIBLE CAuSE SOLuTION 1. Air bubble in pump 3. a. Other components in sample 2. Allow more time for column equilibration between runs SOLuTION K. Inadequate buffering 1. Replace with proper mobile phase b. d.

If necessary. high (Drift usually to higher absorbance. Use HPLC grade solvents. 5. 10. Reset baseline. use heat exchanger before detector NORMAL PROBLEM BASELINE dRIFT 2. especially when changing mobile phase 7.) window. sparge temperature fluctuation.IV. Change wavelength to UV absorbance maximum 9 . 3. 2. Mobile phase contaminated. Most often affects refractive index and conductivity detectors. (Even small changes cause cyclic baseline rise and fall. Mobile phase mixing problem or change in flow rate 6.) 4. To avoid. Plugged outlet line after detector. Contaminant or air buildup in detector cell 3. Control column and mobile phase temperature.) 9. PROBLEMS WITH THE CHROMATOGRAM (continued) L. run 10-20 column volumes of new mobile phase before analysis 7. Check make-up of mobile phase. Use highest grade chemicals and HPLC solvents 8. Detector (UV) not set at absorbance maximum but at slope of curve 5. 9. or UV detectors at high sensitivity or in direct photometric mode. Flush with intermediate strength solvent. deteriorated. Baseline drift POSSIBLE CAuSE SOLuTION 1. (Gradient analyses canaggravate problem. Nonhomogenous mobile phase. to detector manual to replace producing noisy baseline. purity salts. Use new mobile phase when dynamic range of detector is exceeded. or prepared from low quality materials 8. Correct composition / flow rate. Slow column equililbration.) with helium during use. routinely monitor composition and flow rate 6. and additives. Mobile phase recycled but detector not adjusted 10. Flush cell with methanol or other strong solvent. If necessary. Column temperature fluctuation. Strongly retained materials in sample (high k’) can elute as very broad peaks and appear to be a rising baseline. Use guard column.) 1. clean cell with 1N HNO3 (never with HCI. flush column with strong solvent between injections or periodically during analysis. Unplug or replace line. Refer (High pressure cracks cell window. Degas rather than cyclic pattern from mobile phase before use. 4.

Incomplete mobile phase mixing less viscous solvent 4. Leak Normal Problem BASELINE NOISE 1. Change pump seals if necessary 3. Other electronic equipment on same line 6. See section III. 2. or pump 2. Check make-up of mobile phase. unusual noises. Temperature effect (column at high 4. Mix mobile phase by hand or use Normal Problem BASELINE NOISE 3. Check for loose fittings. Reduce differential or add heat temperature. Select and use only miscible solvents 4. or prepared from low quality materials 3. unusual noises. Mobile phase contaminated. Baseline noise (regular) POSSIBLE CAuSE SOLuTION 1. Air bubbles in detector continued on next page 10 . Purge detector. detector or recorder to determine if source of problem is external. Flush system to remove air from detector cell or pump 2. Isolate LC. Baseline noise (irregular) POSSIBLE CAuSE SOLuTION 1. Isolate detector and recorder electronically. Correct as neccessary 6. Check system for loose fittings. See section Ill. Check pump for leaks. Mobile phase solvents immiscible 4. Detector/recorder electronics 3. PROBLEMS WITH THE CHROMATOGRAM (continued) M. salt build-up.IV. detector unheated) exchanger 5. Check for detector cell leak 2. Pump pulsations 5. Install backpressure device after detector 5. Change seals if neccessary. salt build-up. Degas mobile phase. Air trapped in system 6. Flush system with strong solvent 6. Check pump for leaks. Refer to instruction manual to correct problem 5. Incorporate pulse dampener into system N. detector cell. deteriorated. Air in mobile phase. Leak 1.

010 in. 10 µL vs. Repair or replace the mixer or mix off-line if isocratic O. PROBLEMS WITH THE CHROMATOGRAM (continued) N. Weak detector lamp 9. Reduce response time or use volume too large smaller cell c. Detector response time or cell b.IV. 100 µL) or 1:10 and 1:100 dilutions of sample b. salt build-up. Replace lamp 9. Column overloaded 5. a.. ID tubing as practical d. Extra-column effects: a. Reduce response time Normal Problem BROAd PEAkS 11 . Detector cell contaminated (even small amounts of contaminants can cause noise) 8. Replace column 10. Mobil phase mixer inadequate or malfunctioning 7. Mobile phase flow rate too low 3. and unusual noises. Check pump for leaks. Check for loose fittings.007detector too long or ID too large 0. Adjust settings 4. Adjust flow rate 3. Mobile phase composition changed 1. Clean cell by flushing with 1N HNO3 (never with HCI) 8. Inject smaller volume (e. Broad peaks POSSIBLE CAuSE SOLuTION 1. Prepare new mobile phase 2. Use as short a piece of 0.g. Baseline noise (irregular) continued POSSIBLE CAuSE SOLuTION 7. Tubing between column and c. See section III. Leaks (especially between column and detector) 2. Detector settings incorrect 5. Change seals if necessary 4. Column leaking silica or packing material 10. Recorder response time too high d.

Column contaminated / worn out. Peak represents two or more poorly 10. Column temperature too low 11. Replace guard column 8. Prepare new mobile phase 2. Increase temperature. Broad peaks (continued) POSSIBLE CAuSE SOLuTION 6. Open inlet end and fill void or replace column 10. Do not exceed 60 °C unless higher temperatures are acceptable to column manufacturer 12. Replace column with new one of same type 9. If analytical column is obstructed. Use appropriate restoration procedure. column may be fouled with strongly retained contaminants.IV. If problem persists. Normal Problem LOSS OF RESOLuTION 12 . Buffer concentration too low 7. Detector time constant too large P. Increase concentration 7. Obstructed guard or analytical column 1. inlet is probably plugged. PROBLEMS WITH THE CHROMATOGRAM (continued) O. Remove guard column and attempt analysis. Guard column contaminated/worn out 8. Use smaller time constant 12. Low plate number 9. Replace guard if necessary. If problem persists. Mobile phase contaminated / deteriorated (causing retention time to change) 2. Change column type to improve resolved compounds separation 11. Change frit or replace column. Loss of resolution POSSIBLE CAuSE SOLuTION 1. Void at column inlet 6. reverse and flush.

Use correct connection 4. Detector time constant too large 3. PROBLEMS WITH THE CHROMATOGRAM (continued) Q. Improper recorder connection 13 . Use correct connection 3. if column size allows 4. Improper recorder connection R. Use larger attenuation 2. Reduce sample concentration b. Use smaller time constant 3. use a smaller sample loop or use partial loop filling 3. All peaks too large POSSIBLE CAuSE SOLuTION 1. Detector attenuation too low 2. Increase injection volume.IV. a. Detector attenuation too high 2. Reduce attenuation 2. Increase sample concentration b. Decrease injection volume. Injection size too small 1. All peaks too small POSSIBLE CAuSE SOLuTION 1. Injection size too large 1. a.

Dirty syringe 4. Clear or replace lines C. Supply proper pressure (power) 2. hard to load POSSIBLE CAuSE SOLuTION 1. Jammed mechanism 3. Damaged rotor seal 2. Adjust alignment. Blocked loop 3. Blocked lines 1. A. hard to turn POSSIBLE CAuSE SOLuTION 1. See service manual 3. 2. Blockage 2. Rotor too tight 1. Autoinjector. No air pressure (or power) 2. Faulty controller 1. Valve misaligned 1. Adjust rotor tension B.V. won’t turn POSSIBLE CAuSE SOLuTION 1. Autoinjector. Manual injector. other problems POSSIBLE CAuSE SOLuTION 1. Adjust 3. Manual injector. Repair or replace controller 14 . Valve misaligned 2. Clean or replace syringe 4. PROBLEMS WITH THE INjECTOR These problems are usually detected while you are using the injection valve. Leaky injection valves are discussed in Section III (Leaks). Rotor too tight 3. Replace loop 3. Adjust alignment D. Rebuild or replace valve 2. Clear or replace blocked portion 2.

Detector lamp failing 1. Pressure limit exceeded 2. Check for proper ventilation. This will help you to quickly locate problems. Warning lamps POSSIBLE CAuSE SOLuTION 1. See service manual 3. Shut module off. Column oven problem 3.VI. See section III 2. see service manual C. a. a. adjust c. Spill 1. For example. Solvent smell POSSIBLE CAuSE SOLuTION 1. adjust b. Leak 2. A. Check limit setting. Replace lamp D. Check settings. Other warning lamps 1. See service manual 15 . Check for blockage b. Locate spill and clean up B. You should get in the habit of taking a few minutes each day to expose all of your senses (except taste!) to the LC so that you can get a “feel” for how the LC performs normally. SIGHT OR SOuNd You need to use all your senses to identify LC problems. adjust 2. most of these are included in the preceeding section. Abnormal meter readings POSSIBLE CAuSE SOLuTION 1. Check for overflowing waste container b. often you can smell a leak before you see it. Pressure abnormality 2. “Hot” smell POSSIBLE CAuSE SOLuTION 1. adjust b. See section II 2. Overheating module 1. Check temperature setting. PROBLEMS dETECTEd By SMELL. The majority of problems are identified by sight. a. a.

Mechanical wear 1. Solvent leak / spill 2. Locate and correct 2. PROBLEMS dETECTEd By SMELL. Bearing failure 2. Squeaks and squeals POSSIBLE CAuSE SOLuTION 1. See service manual 2. Poor lubrication 3.VI. See service manual F. SIGHT OR SOuNd (continued) E. Warning buzzers POSSIBLE CAuSE SOLuTION 1. Other warning buzzers 1. See service manual 16 . Lubricate as necessary 3.

Blocked inlet frit 2. a. Filter mobile phase. Filter samples c. In the right-hand column are listed preventive maintenance practices that can reduce the failure rate. a. Degas mobile phase 2. The operator’s and service manuals for your LC may have additional suggestions for preventive maintenance of your model of LC. The numbers in parentheses are suggested intervals between maintenance. Reservoir PROBLEM PREVENTIVE MAINTENANCE 1. a. Filter mobile phase. Avoid mobile phase pH > 8. (Most silica-based columns) b. Blocked frit 1. kEy PROBLEM AREAS ANd PREVENTIVE MAINTENANCE The chart below lists the most common problems that occur with each LC module. Replace (3-6 mo.5µ filter 2. use inlet-line frit. Filter mobile phase b. Filter samples Column PROBLEM PREVENTIVE MAINTENANCE 1. Gas bubbles 1. Pump seal failure 3. Check valve failure 1. Rotor seal wear 1. Void at head of column 17 . Use precolumn (saturator column) 2. Use in-line filter and/or guard column 2. Air bubbles 2. Don’t overtighten b.VII.) 3. Degas mobile phase Pump PROBLEM PREVENTIVE MAINTENANCE 1.) b. Replace (3 mo. 0. a. Use guard column c. Keep spare Injector PROBLEM PREVENTIVE MAINTENANCE 1.

Bubbles in cell 2. Keep cell clean b. increased detector noise spare lamp 2.) or keep response. Flush buffer from LC and clean when not in use 18 . decreased detector 1. Corrosive/abrasive damage 1. kEy PROBLEM AREAS ANd PREVENTIVE MAINTENANCE (continued) Detector PROBLEM PREVENTIVE MAINTENANCE 1. Replace (6 mo. Use restrictor after cell c.VII. a. Degas mobile phase General PROBLEM PREVENTIVE MAINTENANCE 1. Lamp failure.

19 .com/info/securityguard A universal HPLC guard cartridge system designed to effectively protect your valuable analytical columns and results from the damaging effect of contaminants.no wrenches required From injector contaminants compounds of interest Holder HPLC column uNIVERSAL FIT*: With the patented design. and does not apply to SemiPrep or PREP guard cartridges.can be easily inspected for contaminants HOW IT WORkS*: Universal Fingertight connection to HPLC column . If you are not completely satisfied with the performance of SecurityGuard.WARNING: CONTAMINANTS CAN CAuSE • • • • • • • • High Backpressure Split Peaks Broad Peaks Baseline Noise Baseline drift Loss of Resolution Irreversible Column damage System damage PROTECT yOuR HPLC COLuMN ANd RESuLTS Additional information can be found at www. *Feature applies to analytical-sized guard system only. simply contact Phenomenex within 45 days and keep SecurityGuard for FREE. SecurityGuard can adjust to fit virtually any manufacturer’s female/inverted endfitting. Cutaway view showing Cartridge . Trap contaminants without altering your chromatography.phenomenex. Inc. SecurityGuard is a trademark of Phenomenex.

com/sample If Phenex Syringe Filters do not perform as well or better than your current syringe filter product of similar membrane. return the product with comparative data within 45 days for a FULL REFUND.Phenex Syringe Filters ™ For Sample and Solvent Filtration Prior to Chromatography • Less system down time • Consistent. Please contact your local Phenomenex technical consultant or distributor for availability or assistance. reproducible results • Increased column lifetime Phenex Offers: » Low absorption » Maximum chemical compatibility » Minimum extractables » Excellent flow rate » High total throughput » Low hold-up volume » Certified quality » 100 % integrity tested » Easy pore identification » Bi-directional use Membrane Types RC (Regenerated Cellulose) PTFE (Polytetrafluoroethylene) PES (Polyethersulfone) NY (Nylon) CA (Cellulose Acetate) GF (Glass Fiber) Above syringe filters are non-sterile. . Phenex is a trademark of Phenomenex. Larger quantity purchases at significant savings are available. diameter and pore size. Housing is made of medical-grade polypropylene (PP).phenomenex. Request yours today by phone or visit www. Tip: Try a Sample Pack! The best way to determine if a specific Phenex membrane is suitable for your application. Inc.

Maccles eld..phenomenex. Suite #104 San Juan.com Belgium Maarssenbroeksedijk 13D 3542 DL Utrecht Netherlands +31 (0)30-2418700 +31 (0)30-2383749 beinfo@ phenomenex. For the distributor in your country.com France Parc des Grillons.3 60 route de Sartrouville 78232 Le Pecq Cedex France 01 30 09 21 10 01 30 09 21 11 franceinfo@ phenomenex. Maccles eld. mail: Australia PO Box 4084 Lane Cove.com Netherlands Maarssenbroeksedijk 13D 3542 DL Utrecht Netherlands 030-2418700 030-2383749 nlinfo@ phenomenex.. UK 01625-501367 01625-501796 ukinfo@ phenomenex.: fax: email: mail: 6116_L tel. CA 90501-1430 USA (800) 543-3681 (310) 328-7768 info@ phenomenex. Additional copies are available from: www.com Italy Via M. Est.com Austria Zeppelinstr. 15/D 40013 Castel Maggiore (BO) Italy 051 6327511 051 6327555 italiainfo@ phenomenex.com Canada 411 Madrid Ave. Hurds eld Ind. Cheshire SK10 2BN.com Denmark Gydevang 39-41 3450 Allerød Denmark 4824 8048 4810 6265 dkinfo@ phenomenex.This publication is distributed free of charge.com New Zealand P O Box 31-601 Milford 0741 North Shore City New Zealand 09-4780951 09-4780952 nzinfo@ phenomenex.com Puerto Rico 273 Sierra Morena.com tel. Puerto Rico 00926 (800) 541-HPLC (310) 328-7768 info@ phenomenex. Torrance. CA 90501-1430 USA (310) 212-0555 (310) 328-7768 info@ phenomenex.com.: fax: email: . International Department by telephone. UK 01 247 5405 +44 1625-501796 eireinfo@ phenomenex. Est. NSW 2066 Australia 02-9428-6444 02-9428-6445 auinfo@ phenomenex. Serenari. fax or email: international@phenomenex.com Ireland Queens Avenue.com USA 411 Madrid Ave.com United Kingdom Queens Avenue. Torrance. 5 63741 Aschaffenburg Germany 01-319-1301 01-319-1300 anfrage@ phenomenex.com Germany Zeppelinstr.com Phenomenex products are available worldwide.com Luxembourg Maarssenbroeksedijk 13D 3542 DL Utrecht Netherlands +31 (0)30-2418700 +31 (0)30-2383749 nlinfo@ phenomenex. Cheshire SK10 2BN. Bat.: fax: email: mail: tel. Hurds eld Ind. contact Phenomenex USA. 5 63741 Aschaffenburg Germany 06021-58830-0 06021-58830-11 anfrage@ phenomenex.

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