Design an experiment to determine the effect of carbon dioxide concentration on the rate of photosynthesis.

Answer Aim/objective: To determine the effect of carbon dioxide concentration on the rate of photosynthesis. Problem statement : What is the effect of carbon dioxide concentration on the rate of photosynthesis? Hypothesis When the concentration of carbon dioxide increases, the rate of photosynthesis also increases until the rate becomes constant. Variables Manipulated : Concentration of carbon dioxide Responding : The rate of photosynthesis// The number ofbubble released per minute Constant : Light intensity, temperature/ Size of plant Apparatus and materials : Materials : Elodea/Hydrilla , different concentration of sodium bicarbonate, distilled water Apparatus : Beaker, boiling tube, clip, table lamp, filter funnel Technique : Count and record the number of bubbles produced / released in 20 minutes using a stopwatch . Procedure : 1.Diagram of experimental setup with at least 5 functional labels. 2.Fill a boiling tube with 150 ml of 1% sodium hydrogen carbonate solution. 3.Fix a paper clip / plasticine to a sprig of aquatic / Hydrilla sp. plant. 4.Submerge / put the aquatic plant into the boiling tube containing 1% sodium hydrogen carbonate solution. 5.Put the boiling tube into a water bath at room temperature (20-30oC). 6.Place the boiling tube at distance of 50 cm from a lighted bulb.

7.Put aside the boiling tube for 5 minute (for the aquatic plant to adjust). 8.Start the stopwatch. 9.Count the number of bubbles release in 20 minutes. 10.Record the result is a table. 11.Repeat steps 2 until 10 using 2%, 3% and 4% of sodiumhydrogen carbonate solutions. 12.Calculate the rate of photosynthesis. (Rate of photosynthesis = Number of bubbles/time) 13.Precaution : Count the bubbles only after the rate becomesconstant. Data Include the following titles with units:
-Concentration of sodium hydrogen carbonates solution (%) -Number of bubbles released in 20 minutes -Rate of photosynthesis (number / minute)

Conclusion: When the concentration of carbon dioxide increases, the rate of photosynthesis also increases until the rate becomes constant. Hypothesis is accepted.
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