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Calculate the colony forming units (cfu) per ml. of starter culture. Choose a plate with between 50 and 150 colonies. Calculate the cfu/mL of culture by using the following proportion. # of colonies x dilution of plate X ------------------------------------------------ = -------------volume of culture on plate 1 ml. For example, if you counted 24 colonies on the 105 dilution plate you would use the following proportion and solve for X using the following steps. 1) set up proportion 24 x 105 ------------0.25 X = ------1

2) cross multiply

0.25 x = 1 (24 x 105 )

3) divide to get x alone

0.25 x 1 (24 x 105 ) --------- = ----------0.25 0.25

4) use calculator to solve

(24 x 105 ) ------------0.25

5) put answer in scientific notation X = 9600000 X = 9.6 x 106

000.1 ml x 10^5 = 46. this is true for many strains of Escherichia coli and other organisms commonly used in the lab.1 ml x 10 x 10 x 10 = 800. 40 for the 10^-6 dilution.000 cfu/ml or 0.Science Research 7 Calculate the cfu/mL of culture. A CFU is basically just a single. 10^-6.Chosen by Voters CFU = colony forming unit. • 3 years ago We did an experiment where we did 10^-5 .8 x 10^6 B: 46/0.000 cfu/ml or 14. Show all your work! number of colonies __42__ dilution of plate __106___ number of colonies __57__ dilution of plate __105___ number of colonies __75___ dilution of plate ___104___ number of colonies __62___ dilution of plate ___106___ A: 80 cfu/0. How do I get the Cfu/ml?? • • 10 months ago Report Abuse by bradlepe Member since: March 25. 2008 Total points: 8.700. the .7 x 10^6 Note: cfu means colony forming unit which is equivalent to a bacterium as long as the bacteria are single-celled and are not clumps or groups when plated. and 8 for the 10^-7. 10^-7 dilutions. viable cell that's capable of dividing and form a colony after some incubation period.1 ml x 10^4 = 14. So once your incubation is done.115 (Level 5) • • Add Contact Block Best Answer .000 cfu/ml or 46 x 10^6 C: 147 cfu/0. For the first tube of bacteria I grew 400 colonies on a plate after pipetting 100 ul onto the plate with a 10^-5 dilution.

Hope this helps! • 10 months ago Best Answer . So at dilutions of 10^-5: CFU/mL = (400 * 10^5)/(0.Science Research 7 number of colonies present should be the same as the number of CFUs that were originally plated.1 mL) = 4*10^8 CFU/mL At 10^-6: CFU/mL = (40 * 10^6)/(0.1 mL = 440 cfu/mL But you did a 50 fold dilution so 440 x 50 = 22000 cfu/mL in the original sample • • • 1 year ago Report Abuse 1 person rated this as good Asker's Rating: Asker's Comment: thanks Not the right answer? Try Yahoo! .Chosen by Asker 44 colonies / 0.1 mL) = 4*10^8 CFU/mL At 10^-7: CFU/mL = (8 * 10^7)/(0. CFU / mL = (# colonies)*(dilution factor)/(volume plated in mL) Dilution factor is the same as the dilution you've written above.1 mL) = 8*10^8 CFU/mL. except the exponent should be positive instead of negative.

1..Chosen by Voters let's call the culture "C" and the dilution "D". Calculate the CFU/100 ml in the original culture.. what was the original CFU/ml in the milk? please show your work! Thanks! :D • • 2 years ago Report Abuse by m w Member since: May 09. 100 mL C x (1.127 (Level 7) Badge Image: • • Add Contact Block Best Answer .Science Research 7 Biology CFU calculations A culture was diluted by adding a 0.1 ml of this dilution was plated out.. yielding 73 colonies.1 mL D) = 7.1 C mL = 1.. 200 microliter of milk was mixed with 9.200 mL ..0 mL ?) next. 2. so the answer is 7.you know this...so.1 mL D 0..8 ml of water.1 C + 0..1 mL C) x (73 CFU / 0.0 mL D / 0...1 ml aliquot to 0.0 mL D and you want to know how many CFU's in 100 mL of C...0 mL "D" right? (D is 0.3x10^5 CFU / 100 mL ************ couple of quick conversions. Then. 0. 73 CFU = 0. 2006 Total points: 132. and 50 microliter of the dilution was plated. And 0.9 ml of water..3x10^5 CFU.9 water = 1.1 C went into 1... If there were 141 colonies on the plate. 200 microliters x (1 mL / 1000 microliters) = 0.

.050 mL dilution = 141 CFU and you want to know how many CFU's per 1 mL.0 mL dilution / 0. you know this.. Member since: June 15.200 mL milk) x (141 CFU / 0. .8 mL = 10.200 mL + 9.050 mL 0..41x10^5 CFU / mL ******* do you need help with the equations above? • • 2 years ago Report Abuse 100% 2 Votes • 1 person rated this as good Not the right answer? Try Yahoo! Search Search Yahoo! for þÿ Search There are currently no comments for this question. Other Answers (1) • by hcbioche... * You must be logged into Answers to add comments. next... Sign in or Register.905 (Level 7) Badge Image: o o Add Contact Block . 2006 Total points: 40..200 mL milk = 10..41x10^5 CFU so the answer is 1..0 mL dilution 0.050 mL dilution) = 1.. 1 mL milk x (10.0 mL of diluted solution.so.Science Research 7 50 microliters x (1 mL / 1000 microliters) = 0..

so 730 X 10 = 7300 CFU/mL of the original culture. 73 CFU/ 0.8 mL is a 50-fold dilution (10/. multiply7300 X 100 = 730.000 CFU/mL of the original milk.2 = 50). 141 CFU/0. so 2820 X 50 = 141. Since you want CFU/100 mL.1 mL = 730 CFU/mL by that is a 10-fold dilution.000 CFU/100mL 2.Science Research 7 1.05 mL = 2820 CFU/mL of the 50-fold dilution. o o 2 years ago Report Abuse . 200 uL to 9.

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