Eloisa Atienza Frances Jane Hermo Nick Rainier Santos Assignment #2: Proteins Amino acid analysis of an octapeptide revealed

the following composition: 2 Arg, 1 Gly, 1 Met, 1 Trp, 1 Tyr,1 Phe, 1 Lys. The following facts were observed: a. Edman degradation gave

April 20, 2009 Chemistry 40 Biochemistry Evangeline Amor

b. CNBr treatment yielded a pentapeptide and a tripeptide containing phenylalanine. c. Chymotrypsin treatment yielded a tetrapeptide containing a C-terminal indole amino acid and two dipeptides. d. Trypsin treatment yielded a tetrapeptide, a dipeptide, and free Lys and Phe. e. Clostripain yielded a pentapeptide, a dipeptide, and freePhe. What is the amino acid sequence of this octapeptide? CNBr attacks the peptide bond of Met at the carboxylic group. Gly ± Arg ± Lys ± Trp ± Met + Tyr ± Arg ± Phe Chymostrypsin attacks the peptide bonds of amino acids with aromatic ring at the carboxylic group. Gly ± Arg ± Lys ± Trp + Met ± Tyr + Arg ± Phe Trypsin attacks the peptide bonds of Arginine and Lysine at the carboxylic group. Gly ± Arg + Lys + Trp ± Met ± Tyr ± Arg + Phe Clostripain attacks the peptide bond of Arginine at the carboxylic group. Gly ± Arg + Lys ± Trp ± Met ± Tyr ± Arg + Phe

Structure of the octapeptide: Gly ± Arg ± Lys ± Trp ± Met ± Tyr ± Arg ± Phe

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