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Lecture 21-Vibrio cholerae

Lecture 21-Vibrio cholerae

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Published by: Khalifa Sifaw Ghenghesh on Apr 26, 2011
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05/18/2012

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FAMILY: VIBRIONACEAE

Prof. Khalifa Sifaw Ghenghesh

3 Important Genera: ‡ Vibrio ‡ Aeromonas, ‡ Plesiomonas
‡ Oxidase +ve Habitat:

Vibrio cholerae
I. V. cholera O1:
‡ Agglutinate in Antisera Directed Against O1 Antigen. ‡ Produce Enterotoxin.

2. Non-Toxigenic V. cholera O1:
3. Non O1 V. cholera (NCV, NAV):

II. V. cholerae O139

Vibrio cholerae
Gram-negative, curved rods

Vibrio cholerae
Leifson flagella stain

Vibrio cholerae
curved rods with polar flagellum (EM)

V. cholerae O1
--------------------------------------------------Biotye Haemolysis (SRBC) VP El-tor + + Classic ---

Resistance to PB + ----------------------------------------------------

VIRULENCE FACTORS:
‡ Enterotoxin: CT ‡ Adherence: Ability to Attach to the Microvilli at the Brush Borders of the Epithelial Cells.

EPIDEMIOLOGY:
‡ Causes Widespread Disease. ‡ Human Carriers: ‡ Modern International Travel:

PATHOGENESIS & CLINICAL INFECTION:
‡ ‡ ‡ ‡ ‡ Infectious Dose: Incubation period: Abrupt Onset of Diarrhoea & Vomiting Stool > Watery (Rice Water), Odorless Fatality Rate:

LAB. DIAGNOSIS:
‡ Isolation & Identification of the Organism. ‡ Media: TCBS ‡ Transport Media:

TREATMENT:
‡ Prompt Replacement of Fluid & Elecrolytes ‡ Tetracycline and others

PREVENTION & CONTROL:
‡ Adequate Sewage Treatment and Water Purification Systems. ‡ Vaccination: ??

Typical Vibrio cholera contaminated water supply

Vibrio parahaemolyticus
‡ Halophilic vibrio ‡ Associated with seafood ‡ Gastroenteritis:
± Outbreaks of Food Poisoning

‡ Extraintestinal infections > wounds
‡ Virulence Factors: ± Haemolysin >
± heat-stable cytotoxin ± Heat-labile enterotoxin ± Adherence to human intestinal cells

Aeromonas species
A. hydrophila, A. sobria & A. caviae
‡ Enterotoxins, Cytotoxins, Haemolysins, Etc« ‡ Gastroenteritis, Septicaemia, and Skin & Wound Infections

TREATMENT
‡ TMP-SMX, Tetracycline, Gentamicin ‡ Resistant to F-Lactam Antibiotics.

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