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I.

INTRODUCTION
Diagnostics plays prominent role in the field of Medicine. Without
proper diagnosis, proper conclusions regarding Medical treatment/
surgery can not be obtained. Similarly preparation of reagents of purest
quality is also essential. Thus Medical Lab Technician Course is attaining
importance. Clinical studies in the fields of Medicine, Pharamacutical
Industries, Nutrition etc. also require the technicians.

The Medical Laboratory Technician Couse is designed to train
man power to carry out medical laboratory technical work in various
departmens in medical and pharmacy colleges, peripheral laboratories,
research and diagnostic centres, to setup and run own clinical laboratory.

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II. Objective's of the Course
1. To train the student to work in diagnostic labs.
2. To train the students to work in manufacturing units of diagnostic
reagents.
3. To assist the qualified experts in these fields
4. Train the students to understand the organisation of Hospitals,
Research Laboratories etc.
5. To train the students to attend to analytical work and R&D work in
drug labs and pharmaceutical industries.

III. Skills to be provided
1. Handling of the Apparatus
2. Accurate measuring, weighing etc.
3. Cleaning of the apparatus
4. Accurate analysis
5. Accurate processing and storage of specimens
6. Accurate Reporting and storage of clinical data.
7. Clinical significance
8. Limitation of the Tests

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IV. Job opportunities
A. Wage Employment
1. Laboratory Technicians in various departments in medical and
Pharmacy colleges
2. Laboratory technician in various departments in diagnostic centers
3. Laboratory technician in hospitals of various sectors
4. Laboratory Technician in quality control R&D sectors of Drug
Manufacturing units
5. Laboratory Technician in Clinical studies in various laboratories
like
1) National Institute of Nutrition
2) Central Drug Research Laboratory
3) Molecular Biology Labs

B. Self Employment
1. Setting of Diagnostic Labs
2. Preparation and sale of readymade reagent kits and media
3. Distribution of lab chemicals, glasswares, lab instruments and
their spare parts etc.

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V. SCHEME OF INSTRUCTION AND EXAMINATION
Ist YEAR
Part A Theory Practicals Total
Periods-Marks Periods-MarksPeriods-Marks
1. English 185 75 - - 185 75
2. GFC 185 75 - - 185 75
Part B
3. Vocational Subjects
Paper I-Biochemistry 160 50 160 50 320 100
Paper II-Microbiology &
Pathology 160 50 160 50 320 100
Paper III-Anatomy &
Physiology 160 50 160 50 320 100
4. On the job training - - 210 50 210 50
Total 840 300 690 200 1540 500

SCHEME OF INSTRUCTION AND EXAMINATION
IInd YEAR
Part A Theory Practicals Total
Periods-Marks Periods-MarksPeriods-Marks
1. Communication
skills/english 185 75 - - 185 75
2. GFC 125 50 60 25 185 75
Part B
3. Vocational Subjects
Paper I-Biochemistry 160 50 160 50 320 100
Paper II-Microbiology &
Animal Care 160 50 160 50 320 100
Paper III- Pathology 160 50 160 50 320 100
4. On the job training - - 210 50 210 50
Total 790 275 750 225 1540 500
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Vocational Subjects Paper I-Biochemistry 5 5 10 Paper II-Microbiology 5 5 10 & Animal Care Paper III. 6 2. SCHEME OF INSTRUCTION PER WEEK FOR VOCATION COURSES IST YEAR Part A Theory Practicals Total 1. 6 2. Communication skills/english 6 . Vocational Subjects Paaper I-Biochemistry 5 5 10 Paper II-Microbiology 5 5 10 & Pathology Papaer III-Anatomy & Physiology 5 5 10 4. GFC 4 2 6 Part B 3. GFC 4 2 6 Part B 3. On the job training Total 25 17 42 SCHEME OF INSTRUCTION PER WEEK FOR VOCATION COURSES IInd YEAR Part A Theory Practicals Total 1. Communication skills/english 6 .pathology 5 5 10 Total 25 17 42 5 .

Pathology Part C 4. On the Job training IInd YEAR Part A 1. General Foundation Course Part B 3. Communication Skills in English 2.Anatomy & Physiology Part C 4.Microbiology & Animal Care Paper III . SYLLABUS Ist YEAR Part A 1.Microbiology & Pathology Paper III .Biochemistry Paper II . Communication Skills in English 2.Biochemistry Paper II . General Foundation Course Part B 3. Vocational Subjects Paper I . On the job training 6 . Vocational Subjects Paper I . VI.

Reception. instrumentation. maintenance and storage of glassware II. Introduction to Bio-chemistry including code of ethics for Medical Lab technicians and Medical Lab Organisation. a) Centrifugation : Principle. 7 . Evaporation : IV) Distillation V) Refluxing VI) Drying different salts and dessicotion 6. SYLLABUS Ist YEAR BIO-CHEMISTRY THEORY (160 Hrs) 1. application and uses c) Cleaning. Spectrophotometry : Principle and theory. construction. operation. care and maintenance. III. Basic lab operations like - I. Separation of Solids from liquids. principles and laws involved. Water. applications b) Filtration using funnel II. and applications 5. Colorimetry : Visual and photoelectric methods. Registration and bio-chemical parameters investigated 3. drying. Different types of Centrifges care and maintenance. 2. types. Instrumental methods of Bio-chemical analysis : I. care and maintenance. Purity of Chemicals II. Corrosives. Glassware and plastic ware used in a bio-chemical laboratory I. Plastic ware : Brief outline 4. Glass ware : a) Types of glass and composition b) Types of glassware used. applications II. Weighing : Different types of balances used. Chemicals and related substances I. their identifiication. construction.

10. Biological importance. Sources. Deficiency diseases II. Carbohydrates : Definition. Biological importance. Blood urea.III. Hygroscopic Substances 7. Classification. Collection of 24 hours urine and preservation 9. 14. Carbohydrates and lipids I. Iodine value. Urine : Types of Specimens. Prevention. Serum uric acid. qualitative tests II. 8. Fat Soluble Vitamins. Collection of specimens I. Qualitative tests. Daily requirements. Biological importance. Vitamins and Minerals I. Serum creatinine. Collection. calculations 12. Amino acids and Proteins Definition. Units of measurements 11. Precautions during collection. Minerals : Sources. Lipids : Definition. 13. Safety and first aid in lab accidents. Diagnostic tests Blood Sugar. Vitamins : Water Soluble vitamins. classification. Blood : Types of Specimens. saponification value. II. Daily requirements. Deficiency diseases 8 . Classification. Glucose tolerance test. Solutions Types based on solute and solvent. Processing and preservation. Urine biochemical parameters. Acid value. 15. Types based on method of expressing concentration.

9 . Separation of plasma from blood 7. Collection of Venous blood 4. Reception and registration 2.PRACTICAL (160 Hrs) 1. Collection of arterial blood 5. Lab glass ware a) Identification b) Handling c) Care and Maintenance d) Uses 9. Qualitative identification tests for amino acids 14. BIO-CHEMISTRY st I YEAR . Preparation of a) Percentage solutions b) Normal solutions c) Molar solutions 11. Quantitative determination of Serum creatinine 17. Glucose Tolerance test 16. Quantitative determination of Blood urea 16. Preparation of protein free blood filtrate 8. Qualitative identification tests of sugars 12. Quantitative determination of Urine Sugar. Lab instruments a) Centrifuges b) Balances c) Photo Electric colorimeter d) Spectrophotometer 10. Separation of Serum from clotted blood 6. Qualitative identification tests of proteins 13. Collection of Capillaryblood 3. Quantitative determination of Blood sugar 15.

autoclave 2) Filtration Methods 3) Ionising Radiation . drying & Sterilization of Glassware disposal of contaminated material i. Dark Ground Microscope III. capsule.e. Electron Microscope. alcohols. dyes and acids and alkalies 4) Gaseous Methods of sterilization. Microscopy a) Pinciple working and maintainance of compound Microscope b) Principle of Flourescent Microscope. spore. Cleaning.Marks : 50 I. Hot air oven. Sterlization and disinfection . Flamming. incineration b) By Merit Heat-pasteurization. Anaerobic Methods of cultivation of Bacteria. 10 . tyndalisation. Mode of action and uses of important chemical disinfections .classification and Methods of sterilization 1) Principle and Methods of sterilization by heat a) By Dry Heat. halogens. IV. clinical infective material inoculated culture media. V. Handling and Disposal of Biomedical waste. Red Heat. Ist YEAR PAPER II MICROBIOLOGY & PATHOLOGY (Theory) Total Hours :160 Max. Morphology and classification of Bacteria Sp. of cell.Disinfection.Phenol and Phenolic compounds. Inspissation. Historical introduction to Microbiology contribution of famous Scientist in the field of Microbiology in brief a) Anatony Van Lee wen Hook b) Robert Koch c) Edward Jenner d) Louis Pasteur e) Joseph Lister II. flagella.

Potassium tellurite Media.Peptone water.Blood agar.Selenites broth. Processing of clinical specimen collected for Isolation and identification of organism a) Preparation of direct smear and staining b) Different Techniques of inoculation for isolation of bacteria c) Hanging drop preparation and its use d) Inoculation of various media for Bio-chemical reactions VIII.Maconkeys Media e) Selective Media . Methods of Collection of clinical specimen for Micro-Biological investigation (indetail) like sputum. Nutrient broth. a) Basal Media .Classification of Media composition and preparation and uses. TCBS.pettroff'smethod of concentration. swabs. Culture Media . CSF and aspirations VII. Loefflers serum slope.VI. tetrathionatebroth Alkaline peptone water d) Differential Media . Wilson and Blair Media Deoxycholate citrate agar media 11 . chocolateagar c) Enrichment Media .Lowenstcin Jenson Media. Compostion and preparation of staining reagents and different methods of staining a) simple staining b) Gram Staining c) Spore staining d) Capsular staining e) Zeihl Neelson staining f) Albert staining g) Negative staining h) Flagellar staining i) Flourescent staining IX. blood. glucose broth. b) Enriched Media . stool. urine.

PATHOLOGY 1. M. Preparation of anti coagulants - Double oxalate. Robertson cooked Meat Media. Epitheleal cells. crystals. bile broth Sugar Media for Bio-chemical Reaction. motility. bile Pigments etc. WBC. sodium citrate. Nitrate reduction test. Indole test. procedure and principle of tests 2. action of each preparation. charging chamber.f) Blood culture media . precautions. 3. urease.Physical Examination.e. pericardial. materials required procedures. colour Chemical Examination .counting. Thioglycolate media. Haematology - a. Preparation and staining smear for Microscopic Examination 3.Physical Examination Microscopy .P. pleural fluids and CSF.Differential count of Peritoneal. Heparin.Sugar Albumin. uses disadvantages. V. staining.specific gravity PH. uses of the sample and advantages of each methods. Identifying and counting the cells.R. reaction. Microscopic Sediment for RBC. Sputum Analysis . bile salts. gelatin liquifaction. tests. abnormal and normal forms. test. finger puncture and vacutainer methods. PDA. 4. Morphology.Glucose broth. Media and Reagents for differents Biochemical reaction i. parasites Preparation of Reagents. quantity required. Hartleys broth. catalase test. 12 . Pheny alkaline deaminase test. casts. Sabourauds dextrose Agar. Collection of Blood - Methods of collection veinpuncture. citiate. Semen Analysis. Body Fluids . EDTA. triple sugar Iron agar. glucose phosphate broth. Urine .Analysis . POCT (sample collection at bed side) b.Physical Examination . Oxidase.

procedure. advantages of each . preparation. of Tallquist. method. color index. f. formula for calculation and clinical significance. Haemoglobin Estimation - Materials. RBC. Estimation of PCV - Macro & Micro Method.G. ESR - Methods used. clinical significance. diluting fluids. formula for calculation and clinical significances e. normal Morphology and values. MCHC.normal values and clinical significance Estimation of Erythrocyte indices .calculation and importance MCV. procedure. MCH. RDW. advantages and disadvantaes and clinical significance g. WBC Count : Methods (Microdilution and bulk dilution) Materials required. procedure. advantages of each methods. h. cyanmeth aemoglobin and S. centrifuging and reading. procedure filling the tube. Platelet count : Morphology and functions of platelets diluting fluids. d. precautuions. sahlis.c. Alkali haldanis. diluting fluids. factors affecting and clinical significance 13 . Reticulocyte Count : Methods (dry & wet) staining. stages. procedures.

Incubator.Physical Examination & Chemical Examination MICROBILOGY Lab Instructions for Personal Safety precaution Receipt and recording a specimen in the lab and dispatch of specimen Cleaning and care of glassware. seitzfilter Preparation of various Media Preparation of stains and smears Methods of collection of microbidogy specimen-and its importance and processsing 14 . syringes. WBC. waterbath. Handling and care of Microscope Operation of Autoclave. & platelet count ESR stands & ESR estimation PCV & calculation of RBC indices Hb estimation by different methods Urine . Ist YEAR PRACTICALS PATHOLOGY Blood Collection Precaution and smearing techniques and labelling of the sample Preparation of anticoagulants RBC. apparatus. preparation of pasteur Pipettes.

III Hours : 160 Marks : 50 Detailed Syllabus I. Reproductive system 11. Lymphatic System 7. Excretory System 12. tissues . Excretory System 10. Cardio vascular system 4. Sense organs 15 . ANATOMY PHYSIOLOGY THEORY . skin. Blood 3. nose. Cardio vascular System 6.epithelial.IST YEAR MLT PAPER . Bones and Joints 8. Sense Organs Eye. Endocrine system 9. Nervous system 8. Lymphoid System 5. Respiratory System 5. nervous 3. Respiratory system 7. ear. Basics in Anatomy 1. Introduction to Human Anatomy 2. tongue 11. Endocrine System 10. properties of cell. Cell structure. Nervous System 9. Digestive system 6. Reproductive System Basics 1. Introduction to Human Physiology 2. Digestive System and Hepato Biliary System 4. connective muscular.

Phalanges. tarsal bones. carpal bones. vetebrae pieces. ulna.III Hours : 160 Max. Ribs-classification. radius. Stomach Lungs. meta tarsal bones. meta carpal bones. Phalanges. Fallopian tubes 3. fibula. ANATOMY AND PHYSIOLOGY PRACTICALS PAPER . Kidney Liver. humerus. Marks : 50 1. scapula. clavicle. sternum. Intestines Heart. Uterus Spleen. Innominate bone. Femur. Human Organs Brain. Human Skeleton It includes - 1) Names of the Bones 2) Identification points 3) Surfaces (Skull.) 2. patella tibia. Human slides Epithelial Tissue Connective Tissue Muscular Tissue Nervous Tissue Liver Kidney Spleen Pancreas Lymphnodes 16 .

Total count RBC Total count of WBC DLC differential count of Leucocyts) 17 . pulse. Blood Pressure Estimation 5. (Temperature.P. DLC. T. (TC . respiration) Chart 6. Skin testes Ovary Uterus Tonsil Stomach layers Small Intestine Large Intestine 4.R. TC.

Urea cycle 18 . Clinical importance of determination of electrolytes. Electrophoresis : Definition and basic principle involved. general and clinical applications. different types and their techniques. Basic principles. electrophoretic fractionation of serum proteins and lipo proteins. Description of instruments used in these essays. Separation Techniques 1) Chromatography : Definition.Glycolysis and TCA Cycle. construction of Fluorimeter. general technique and clinical applications of Nephelometry. General and Clinical applications 3. Nephelometry : Basic principle. Safety and precautions. Construction of Flame Photometer.Definition. Basic Principles of immuno chemical reactions and immuno assays 1) Radio immuno assays Introduction to radio activity. Fluorimetry : Fluorescence. 4. 2) Lipid metobolism . Instrumental methods of Bio-chemical analysis 1) Flame photometry : Principle. Basic principles and application of Potentiometry II. MLT IInd YEAR THEORY SYLLABUS BIO-CHEMISTRY (160 Hrs) I. Immuno assays . 2) Enzyme linked immuno essays. General and Clinical applications study of electrolytes using Flame photometer. III.P . Radio Pharmaceuticals. Theory. Metabolism : 1) Carbohydratemetabolism . IV. instrumentation. Hormone assays.Oxidation of Fatty acids 3) Protein metabolism . General and clinical applications 2. different types. 2. instrumentation. Principle and theory.

Liver function tests : 1) Basic concepts including normal and abnormal bilirubin metabolism 2) Classification 3) Serum bilirubin determination 4) Vandenbergh test 5) Total proteins and A/G ratio 6) Enzyme estimations as LFT.V. Titrimetric methods of Quantitative determination. Clinical Enzymology : 1) Introduction and Basic Concepts of Enzymes. Renal function tests : 1) Basic concepts and classification 2) Clearance tests 3) Concentration and dilution tests and 4) Urine examination in assessing kidney function VIII. preparation of various solutions used in titrimetric analysis. Gastric function tests : 1) Basic concepts and introduction 2) Techniques of different tests including tubeless gastric analysis IX. Thyroid function tests : 1) Basic Concepts 2) Estimations of various thyroid hormones. Coenzymes and Isoenzymes 19 . Pancreatic function tests 1) Basic concepts and introduction 2) Various tests done and methods including serum amylase determination XI. VI. VII. their interpretations 3) Recent methods of thyroid function tests X.

Chemical laboratory.Serum cholesterol. Quality assurance in Bio . Diagnostic tests a) Lipid profile . HDL Cholesterol b) Glycosylated haemoglobin c) Serum Calcium d) Inorganic Phosphate e) Analysis of hormone metabolites f) Blood gas analysis 20 . Automation and usage of computers in Biochemical Analysis XiV.2) Importance of Enzymes 3) Transaminases 4) Cardiac Enzymes 5) Acid Phosphatase 6) Alkaline Phosphatase XII. General principle b) Internal and external quality control XV. a) Introduction and importance of quality assurance. Body Fluids : 1) Outlines of formations of different body fluids 2) Composition & analysis of CSF including a) CSF Sugar estimation b) CSF Proteins estimation c) CSF Chlorides estimation including interpretation of results XIII.

PRACTICAL SYLLABUS BIO-CHEMISTRY II YEAR 1) Electrophoretic fractionation of serum proteins and lipo proteins. 17) Demonstration of RIA 18) Demonstration of enzyme linked immuno assays 19) Qualitative identification of urine sugars 20) Qualitative identification of urine proteins 21) Qualitative identification of urine BS & BP 22) Gastric juice analysis. 2) Separation of amino acids and carbohydrates by paper chromatography. 3) Determination of plasma prothrombin time 4) Oral glucose tolerance test 5) Estimation of serum calcium and inorganic phosphate 6) Practice and use of automated pipettes 7) Turbidimetric method of determination of plasma fibrinogen 8) Estimation of HDL cholesterol 9) Determination of Urinary 17 ketosteroids and VMA 10) Determination of CPK.Pandy's test.Apelt 13) Demonstration of working of Auto analysers 14) Training of Computer basics 15) Estimation of serum sodium and potassium by Flame photometry 16) Estimation of serum bicarbonate by titrimetric method. Nonne . demonstration of stimulation tests 21 . LDH. GGT and G6PD activities 11) Determination of urine proteins by turbidimetric method 12) CSF analysis .

23) Renal Calculi analysis 24) Biliary Calculi analysis 25) Demonstration of Ion selective electrodes 26) Practice of Liver functions tests. and interpretation 27) Practice of Renal functions tests. and interpretations 28) Practice of Thyroid function tests and interpretations 29) Practice and interpretation of cardiac profile and lipid profile 30) Practice of quality control measures 22 .

4. What are different types of autoanalysers used in a biochemical laboratory. Write about tubeless gastric analysis 23 . Marks : 50 Section . Name different hormones assayed by RIA. How do you classify enzymes? 13. Write the principle of Flame photometry 5. 3. Section . Write about determination of Glycosylated haemoglobin and its clinical importance 12. b) Urea cycle 6.A Note : (i) Answer all the Questions (ii) Each Question carries 2 marks 2 x 10 = 20 1. Write the mechanism of enzyme action. What are transaminases? Give examples. Explain abnormal bilirubin metabolism 14. MODEL QUESTION PAPER THEORY BIO CHEMISTRY -II YEAR (MLT) Time : 3 Hours Max. Mention various clearance tests done to assess renal functioning 7. What is quality assurance? Explain internal qualitycontrol 9. Give the principle of vandenberghtest 8.B Note : (i) Answer any five Questions (ii) Each Question carries 6 marks 5 x 6 = 30 11. 2. Mention lipid profile tests 10. Define a) Glycolysis. List out various gastric function tests. Write the clinical applications of chromatography and electrophoresis.

24 . How do you determine serum amylase? Give the principle.15. Define Primary standard and secondary standard Classify different titrimetric methods 18. Explain basic principles of immunochemical reactions. requirements and method 16. Write about RIA of T4. Discuss about automation in a biochemical laboratory 17.

concentration techniques of stool for Microscope Examination Parasitology . E-Granulosus. Dracunculus Medinensis. complement fixation.IIND YEAR THEORY PAPAER . Gardia. agglutination. Introduction of Iummunology - a) Brief outline of Immunity b) What are antigens c) What are antibodies d) Different-types of antigen and antibody reaction their application in the diagonostic. Leshmania. Wucharia Ban crofti IV. Normal Flora of Micro-organisms in the Human Body.Preparation of Antibiotic discs V. e) Principle and Method of ELISA Tests.Vermicularis. 25 .Morphology and Lab diagnosis of E-Histolytica. Antiboitic sensitivity test . Neutralisation. Ankylostoma - deodenale. II. Preservation Methods of Stock cultures and their importance and principle procedure. Marks : 50 I. III. Trichomonas.II MICRO BIOLOGY & ANIMAL CARE Total Hours : 160 Max. Collection and processing of faeces samples. Enterobius . precipitation. Taenia. RIA. Ascaris Lumbricoidus. Plasmodium. MLT .

Mycology - Morphology cultural characteristics and lab diagnosis a) Candida b) Cryptococcus c) Dermatophyta d) Aspergillus e) Pencillium IX. Dengue. Streptococus. Herpes. Influenza. Rubella. Mycro-leprae.E. Pneumococus b) Gram Negative .staphylococus. VIII. Rabies. Pertusis h) Spirochaetes . HIV. General priciples of animal care . Layout of Animal House XII. Virology - Classification. Anaesthesia for animals. Euthanesia.Diptheria. pathogenic viruses such as Measles. Klebsiella.Treponema. breeding and handling of animal and disposal of animal waste XIII.Enterbacteriaceae .Pseudomonas g) H.feeding.Gonococi. Enterobacter. anthracis d) Gram Negative Bacilli . polio. and frog 26 . proteous.E. shigella. Bacteriodes.VI. clostridium f) Vibriocholera . Rethins. Bacteriological examination and water. and J.Coli. milk & food. B. Vaccines - classification and uses XI. Hepatitis. Meningococci c) Gram Possitive Bacilli . leptospira. X.influenza . Myco-tuberculosis. e) Anarobic Bacterial. General properties and cultivation and imp . pathogens a) Gram Positive . Brief outline of Morphology cultural characteristics and lab diagnosis of imp. salmonella. Borrelia i) Actinomyes & Nocardia VII. Mumps.B.Coryn.

Common disease of the lab animals preventive. Techniques of drawing a blood from animals XV.XIV. aspects and disposal of animals and related Malerial 27 .

Bacteriology of food Mycology 11.staphylococus. stool. Wet preparation of faecal sample for ova and cyst. Concentration techniques of stool for ova and cyst. Proteus. Salmonilla. 4. sputum. Procedure of techniques of sputum for AFB. CSF. E. 3. urine. swabs.IInd YEAR MICROBIOLOGY PRACTICALS Total Hours : 160 Max. Fungal culture Serology 28 . Psudomonas. Identification of organisms with Biochemical reactions of common organism like . Parasitology . 12.coli . 5. Antibiotic Sensitivity tests 7.Marks:50 1. shigella. 6. Fungal examination by wet preparation 13. Bacteriology of water 9. swabs etc.Klebsiella. Collection of specimen for fungal examination like skin scrapings. Collection of clinical materials like blood.collection. Identification of ova and cyst in stool sample. Preservation of stock culture 8. preservation and transportation of faecal material for examination of parasites. MLT . 2. Procedure of skin clipping of Leprae Bacilli. Bacteriology of Milk 10.

TPHA Virology 22. Brucella Agglutination test 17. Techniques of drawing of blood from animals 28. breeding and handling of Animals 27. Weil felix test 18. Paul Bunnel test 19.and preventive aspects.14. RA test 20. VDRL Tests 15. Western blot test 24. 29 . Incubation of fertile eggs and innoculation by various routes 25. Preparation of widal Antegens & widal tests 16. Anesthesia for animals 29. 26. Common diseases of the lab animals . CRP test 21. ELISA test 23. Model layout of Animal House. Feeding.

dukes. Gemsia. procedure materials. method and selection of stain. cuithont crush artefacts staining and clinical significane. Special stains on peripheral blood smear and bonemarrow smears- Ramanoskys stains. normal values.Methods used. IV. clinical significance V.slide method. Ivy's procedure Normal value. identifying and counting the cells.Principle.THEORY PATHOLOGY Total Hours : 160 Max.counting and identification of cells . procedure. PAS (Periodic Acid Schiff) . clinical significance b) Cloting time . III.Preparing smears. WBC. Microfilaria. Leishman.Marks : 50 I. materials procedure. Buffer Solution. procedure for smears and staining clinical significance and limitation.methods. Bone Marrow Smear . Sickle Cell Preparation .methods. Differential Leucocyte Count . cover glass method and staining. normal values. observation reporting.Malarial Parasite. Absolute Eosinophil count . factors affecting. Platelets and Haemop araasites. Coagulation Tests a) Bleeding time . procedure and Methods. factors affecting coagulation clinical significance 30 .Materials.Normal values. Preparation of Blood smear examination - Making ideal films . VI.III .Preparation. diluting fluid. Lee & White. Identification of Hemoparasites . interpretation. Morphology. II.IIND YEAR PAPER . MLT . Leishman making thick and thin films procedure and identification of parasite. Morphology of RBC. Mycloperoxidase stain. wrights. Osmotic fragility test . Materials.

Autopsy . X. Basics of coulter counter IX. Microtomes and Knifes XI. L. materials reproting. c) Prothrombin time (PT) d) APTT . clinical significance and titration. procedure.Aims & methods of performing Autopsy cleaning. safety in the lab 31 . Buffy coat preparation . Musuem Techniques Labelling & storage of specimens Methods of color maintenance Presentation of specimen Mounting labelling and cataloging the specimen Maintenance and cleaniness of the Museum Disposal of waste. Cleaning autopsy instruments.E. VIII.LE Cell Test.Principle. Processing and preparation of Histopathology. suturing and retaning the body. Histopathology - 1) Biopsy 2) Processing of tissue a) Fixation b) Dehydration c) Clearing d) Impregnation e) Mounting f) Declacification of Bone g) Routine Paraffin staining h) Immuno histochemists 3. tables and rooms. Microfilaria Abnormal cells. cell Test . preservation of organs.in Detail VII.

their inheritance and antihodies 3) ABO Blood group systems 4) RH Blood group system 5) Techniques of grouping and cross matching 6) Blood collection.XII.a ) direct b) indirect 32 . Preservation and maintaining of Records 7) Coombs Test . Immuno Haematology and Blood Banking i) Introduction 2) Human blood group antigens.

blocks & slides Histopathology specimens and process Preparation of form section material III.Glass ware Microslides & corverstips Sample collection bottles Micropathology Cytology. stop watch Glass Makers Simple balance & colorimeter Water bath . Automatic Tissue Processer Microtome & Knives Centrifuge Hot air overn & Incubator Busm beaker.Direct & indirect methods 33 .P. Maintenance & preservation of Cytology slides M. IV. Immuno Haematology & blood banking ABO blood grouping techniques RH Factor Coombs test . IInd YEAR PATHOLOGY PRACTICAL I.for tissue flotation Knowledge Maintenance & cleaning Care about tissue equipment II.

Cytology Fixations used Fluid preparation for cytological exam Slide preparation and staining Pap staining Mounting and preservation VII. BT. Mukerjee 3 volumes 3. Bone Marrow Smears Preparation & Staining IX. Processing of tissue Fixation Dehydration Clearing Impregnation Mounting Decalcification 3. Sickle Cell Preparation VIII. Ramnik sood 2. Talib 4. Mounting of museum specimens VI.V. Cougulation Test. WHO Lab Manual 34 . Praful Godkar 5. CT. Recommended Books 1. Histopathology 1. Fixation of biopsy tissue 2.

Water bath 1 10. Semi auto analyser 1 Glassware 1. Electrophoresis apparatus 1 20. Computer 1 21. 100 2. Physical Balance 2 12. Refrigerator 165 lit. 1 7. List of Equipments Biochemistry 1 1. Centrifuge tubes 35 .VII. P Meter 18. Hot Plate 1 2. Gas Cyllinder with Burner 1 3. Spirit lamps 15 4. Analytical Balance 5 11. Test tubes 18 x 150 mm . 100 15 x 125 mm . Electrical Centrifuges 1 6. Hand Centrifuges 5 5. Colorimeter 1 8. 100 15 x 150 mm . Incubator 1 19. Typewriter 13. Hot air oven 1 9. Spectro Photometer 15. Flourimeter 1 H 17. Flame photometer 1 14.

1. 5 Nos 25 ml . Pipettes a) Volumetric-capacity 2 ml . 0. 5 Nos b) Serological blow out type 1 ml 1/100 . 5 Nos 10 ml . 4 50 ml . 5 nos.2 ml 1/100 . 10 nos.10 ml 1/10ml . 16 x 100 mm . 0. 2 ml 1/10 ml . 5 nos. 5 ml 1/100 . 10 nos. 4 6.5 ml . Burettes 25 ml . 6 Nos 20 ml . 5 nos. 20 nos. Reagent Bottles 60 ml . c) Ostwald pipettes 0. 120 ml . 10 nos. 5 nos. 200 3. Fol in sugar tube 30 4. 4 nos. 0.2 ml . 4 7. 250 ml . Beaker-glass and polypropylene 250 ml 5 5. 6 Nos 5 ml . 10 nos. 36 . 4 Nos.1 ml 1/100 ml . 0. 10 nos.1 ml . 2 ml 1/100 .

2 nos. 10 nos. 500 ml . Filter Paper . Dropper bottles 30 ml 5 nos. 10 nos. 500 ml . 40 ml . 6 nos. 9. 5 8. 250 ml . Stoppered graduated Test Tubes 15 ml . Glass slides 5 boxes 19. 6 nos. 10 nos. Whatman Filter Paper 46x57 cm No 1 . Mortar/Pestle . 100 ml . 15. 1000 ml . Cotton (absorbant) 5 Rolls 18.20 sheet No 2 . 1 Ream 16. 50 ml . 5 nos. 12. Round Bottom flask 500 ml . 1 no. 10 nos. Distillation assembly (glass) Complete set 1 13.Ordinary . 10 nos. 1 no. 1000 ml . 10 nos.10 sheets 17.6 Nos 10. 10 nos. 11. 5 nos. 20. 100 ml . 37 . 50 ml . Plastic Wash bottles 50 ml. Condensor 1 14. Watch glass (Assorted sizes) . Volumetric Flasks 25 ml .

Microbiology 1. Autoclave . 1 7. 10 nos. Centrifuge . 5 21. 2 3. Student Microscope . Reagent bottles . 1 12. 1 10. 10 23. 1 16. 5 38 . 20. 1 ml . 100 x 17 . Spatulas . Electrophoresis apparatus .100 nos 18. 5 ml . 10 nos. Water Bath . 1 13. Refrigerator . 12 22. 1 14. Pipettes 10 ml . 1 6. Anaerobic Jar . 1 15. 5 2. 10 nos. 1 11. Deioniser . VDRL Rotator . Pipette washer . Petri dishes . Wash bottles . 1 9. Analytical Balance . Vaccum Pump . 1 4. 150 x 19 -100 100 x 12 -100 19. Hot air oven . Test Tubers . 1 17. Measuring Cylinders 50 ml . Distil water plant . Inspissator . 1 8. 1 5. Incubator .

Chemical Balance 1 8. 10 10 ml .Pathology 1. Syringe 20 ml . Stopwatch 1 10. Water Bath 1 7. Incubator 1 4. ESR stand 5 13. Spiritlamp 2 17. Centrifuge 1 5. Hot plate 1 9. Urinometer 2 16. 5 39 . 2 18. 5 2 ml . 5 250 ml . Haemocytometer 5 12. Microscope 1 2. 10 5 ml . Hot air oven 1 3. ESR Tubes 5 14. Mortor and Pestle 2 15. Blood cell counter 2 6. Haemometer 5 11. Beaker 100 ml .

All Hospitals 2. Government Head Quarters Hospital 2. University Departments 6.Collaborating Institutions for Curriculum transaction 1. Pharmaceutical Companies and Education Institutes B. All Medical Colleges 3. Regional Research Laboratories 5. Dispensaries 4. On the Job Training Centres 1. A. Medical colleges 5.VIII. PHCs 3. Private labs 40 . All the national laboratories 4. Private Hospitals 6.

SC (BZC) 2) Courses through EAMCET B) Without Bridge Course 1) B. Qualification of Lecturers 1. Biotechnology 5) M. Vertical Mobilities A) With Bridge Course 1) B. MBS Hom/BHMS 3. MLT 2) B.Sc. B. MLT/Biochemistry/Microbiology/Biotechnology (at P.Sc. Microbiology 3) B. MBBS 2.Sc.IX.Sc.Sc. Biochemistry 4) B. level) 41 . Pharmacy 4.Sc.G. M. Microbiology/Biochemistry/MLT X.

Chowrasia .Textbook of Biochemistry 7. Praful-Godkar .Anatomy & Physiology 11. Ramnik Sood . XIII. REFERENCE BOOKS 1.Text Book of Medical Lab Technology 2. Harold Varley .M. K. Chaurasia .Text Book of Medical Lab Technology 3.Histology 12. C. Rama Rao .C. Chatterjee .Human Physiology 8. Lehninger . Indesten Singh .Human Anatomy 9.Gross Anatomy 42 .Manual for Medical Lab Technology 4. Toratora . Samuel .Practical Clinical Biochemistry 5.Text Book of Microbiology 10. Anantha Narayan .Textbook of Biochemistry 6.

VOCATIONALCURRICULUM-2005 (With effect from the AcademicYear 2005-2006) CURRICULUM OF INTERMEDIATEVOCATIONAL COURSE IN MEDICAL LAB TECHNICIAN STATE INSTITUTE OF VOCATIONAL EDUCATION & BOARD OF INTERMEDIATE EDUCATION A.P. Hyderabad 43 . Nampally.

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It emphasized that well planned. FOREWORD The National Policy on Education (1986) while proposing educational reorganization. placed high priority on the programme of vocationalisation of education. the Government of Andhra Pradesh introduced Vocational Education at +2 level with an aim to diversify a sizeable segment of students at the senior secondary stage to the world of work. I am confident that the revised curriculum will attract more and more students into vocational stream and help them train in need-based. Inspired by this vision of the National Policy. The State Government aimed at reducing the pressures on higher education through empowering youth by harnessing their capabilities. The revised curriculum for Vocational Courses at Intermediate Level will come into effect from the Academic Year 2005-06 1st Year and from Academic Year 2006-07 for 2nd Year students. The requirement of skilled manpower industry is being fulfilled by charting a student's career with right options based on aptitude and talent. productive courses leading to gainful employment. systematic and rigorously implemented vocational education will create a distinct stream to prepare students for identified occupations encompassing several areas of activity. The primary aim of vocational courses was to cut across several occupational fields and prepare students with employable skills in organized sectors and self employment. In view of the changing needs of the students and growing demand for a spectrum of skill competencies in the economy. BIE 45 . the Board of Intermediate Education has reviewed the curriculum of Vocational Courses inorder to re-orient them based on their viability and practicability. An right alternative to medical and engineering courses is envisaged in vocationalisation of education in the State. Vocationalisation through re-orientation of educational strategies focused on creating a talent pool of skilled youth who are trained in courses relevant to the market and emerging needs of the various sections of the economy. SHASHANK GOEL Secretary.

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Skills to be provided 2 IV. List of equipment 35 VIII. I. Syllabus 6 [a] Theory [b] Practicals VII. Introduction 1 II. Qualification for Lecturers 41 X. Vertical Mobility 41 [a] With Bridge Course [b] Without Bridge Course XI. CONTENTS Page No. List of Participants 53 47 . Job opportunities 3 [a] Wage Employment [b] Self Employment V. Reference Books 42 XII. [a] Collaboration Institutions for curriculum transaction 40 [b] On the Job Training Centres 40 IX. Objectives of the Course 2 III. Model Question Papers 43 XIII. Scheme of Instruction & Examination 4 VI.